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The map label for this gene is rfbB [C]

Identifier: 194738064

GI number: 194738064

Start: 2962379

End: 2963329

Strand: Reverse

Name: rfbB [C]

Synonym: SeSA_A3064

Alternate gene names: 194738064

Gene position: 2963329-2962379 (Counterclockwise)

Preceding gene: 194734881

Following gene: 194736195

Centisome position: 62.93

GC content: 55.94

Gene sequence:

>951_bases
ATGCAGATTATCATTACCGGCGGGGGCGGCTTTTTAGGCCAGAAACTCGCAAGCGCCTTATTAAACTCATCGCTGGCGTT
TAACGAACTGCTTCTTGTTGATTTAAAAATGCCTGCACGGTTATCAGATTCCCCTCGTTTACGCTGTCTGGAGGCTGACT
TAACCCAGCCGGGCGTGCTGGAGAATGTGATTACCGCTAATACCTCTGTTGTTTATCATCTCGCTGCGATTGTCAGCAGT
CATGCGGAAGACGATTTCGATCTGGGATGGAAAGTTAACCTGGATCTTACCCGCCAGTTACTTGAGGCGTGTCGTCGACA
ACCGCAGAAAATTCGTTTTGTCTTCTCCAGCTCGCTTGCCGTTTATGGCGGTACGCTGCCGGAATGCGTCACCGATACCA
CCGCGCTCACGCCGCGCTCGTCTTATGGCGCGCAGAAGGCCGCCTGTGAACTGTTGGTCAACGACTATACCCGCAAAGGC
TATGTGGATGGGCTGGCGCTGCGTTTGCCGACGATCTGTGTTCGCCCGGGTAAACCAAACCGCGCCGCTTCTTCTTTTGT
CAGCGCGATTATTCGTGAACCGTTGCAGGGCGAGACGACCATCTGCCCGGTGTCGGAAAGTTTGCGGCTGTGGATTTCCA
GCCCGGCGACGGTGATCCATAACCTGTCGCTGGCCGCAACGTTACCCGCGCCTGGCGAGGCGAGCAGCATCAACTTACCC
GGGATCAGCGTAACCGTGGGTGAGATGCTGGAAACGTTGCGTCAGGCGGGCGGTCAGGCGGCGCGCGATCGGGTTACGCA
TCAGCGCGACGAAGGCGTCGAGAAAATTGTCGCCTCCTGGCCGGGACGTATCGATAACCAGCGGGCGCTGGCGTTAGGTT
TTGTCGCCGATAAACGCTTCGATGACATTATCGAACGCTTTCGACAAGATGATATGGAGGGGAGGTCATGA

Upstream 100 bases:

>100_bases
CGAATACCAACCACGGAATACCACGCAGGACGGGCTTGGCTGGTTTAACGCCTGGCGCTAACCCTGAACTACCCTACACA
ACAATAAAGAGGACATCCGG

Downstream 100 bases:

>100_bases
CAAGTTTACCGACCCCAATTATCGGCCTGATCGTTGCCGTTTTCGTTCTGGTATGGCTGGTGTTACGCACCCGTGTTCAT
GCGCTGATCGCGATGCTGGC

Product: NAD-dependent epimerase/dehydratase:Short-chain dehydrogenase/reductase SDR:3-beta hydroxysteroid dehydrogenase/isomerase:dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVLENVITANTSVVYHLAAIVSS
HAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLAVYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKG
YVDGLALRLPTICVRPGKPNRAASSFVSAIIREPLQGETTICPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
GISVTVGEMLETLRQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRFDDIIERFRQDDMEGRS

Sequences:

>Translated_316_residues
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVLENVITANTSVVYHLAAIVSS
HAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLAVYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKG
YVDGLALRLPTICVRPGKPNRAASSFVSAIIREPLQGETTICPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
GISVTVGEMLETLRQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRFDDIIERFRQDDMEGRS
>Mature_316_residues
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVLENVITANTSVVYHLAAIVSS
HAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLAVYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKG
YVDGLALRLPTICVRPGKPNRAASSFVSAIIREPLQGETTICPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
GISVTVGEMLETLRQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRFDDIIERFRQDDMEGRS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=294, Percent_Identity=23.469387755102, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24667531, Length=296, Percent_Identity=25, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34271; Mature: 34271

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVL
CEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCHH
ENVITANTSVVYHLAAIVSSHAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLA
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCHHHEEEHHCCCHH
VYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKGYVDGLALRLPTICVRPGKPN
EECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCC
RAASSFVSAIIREPLQGETTICPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
HHHHHHHHHHHHCCCCCCCEEECCCHHHEEEECCCHHHHHCCEEEEECCCCCCCCEEECC
GISVTVGEMLETLRQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRF
CCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHH
DDIIERFRQDDMEGRS
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVL
CEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCHH
ENVITANTSVVYHLAAIVSSHAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLA
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCHHHEEEHHCCCHH
VYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKGYVDGLALRLPTICVRPGKPN
EECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCC
RAASSFVSAIIREPLQGETTICPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
HHHHHHHHHHHHCCCCCCCEEECCCHHHEEEECCCHHHHHCCEEEEECCCCCCCCEEECC
GISVTVGEMLETLRQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRF
CCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHH
DDIIERFRQDDMEGRS
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]