BacMap

Definition	Salmonella enterica subsp. enterica serovar Schwarzengrund str. CVM19633 chromosome, complete genome.
Accession	NC_011094
Length	4,709,075

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The map label for this gene is dkgB [H]

Identifier: 194737949

GI number: 194737949

Start: 297054

End: 297878

Strand: Direct

Name: dkgB [H]

Synonym: SeSA_A0285

Alternate gene names: 194737949

Gene position: 297054-297878 (Clockwise)

Preceding gene: 194736696

Following gene: 194738242

Centisome position: 6.31

GC content: 50.18

Gene sequence:

>825_bases
GTGATTAAAAAGAGGAATTCTATGACTATCCCTGCATTCGGTTTAGGTACTTTTCGCCTGAAAGACGACGTGGTTATCGC
TTCTGTGAAAACGGCGCTTGAACTGGGCTACCGTGCTGTCGACACTGCGCAAATCTATGATAATGAAGTTGCTGTTGGAC
AGGCGATTGCTGAGAGCGGCGTACCGCGTAATGAGCTGTACATCACCACTAAGATCTGGATTGAAAACCTTAGCAAAGAC
AAGCTGATCCCAAGCCTGAAAGAGAGCCTGAAAAAACTGCGTACCGATTATGTCGATCTGACGCTGATCCACTGGCCATC
TCCGGGTGATGCCGTGTCTGTCGAAGAATTTATGCAGGCATTGCTTGAAGCTAAAAAACAAGGGCTGACTCGCGAAATTG
GCATTTCAAACTTCACCATTCCGTTGATGGAAAAGGCTATTGCCGCGGTGGGCGTAGACAATATCGCCACCAACCAGATT
GAGCTATCTCCGTATCTGCAGAATCGTAAGGTGGTTGACTGGGCGAAAGCGCACGGTATCCATATCACCTCATACATGAC
GCTGGCCTATGGTAAGGCGCTGAAAGATGAAGTGATTGCACGTATCGCTGCTAAACATAATGCGACGCCGGCGCAGGTTA
TTCTGGCATGGGCAATGGGTGAAGGTTACTCCGTGATCCCTTCATCCACCAGGCGTGAAAACCTGGCAAGCAATCTGCTG
GCGCAGGACCTGCATCTGGATGCTGAAGATAAAAATGCGATCGCGGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGA
AGGTCTGGCCCCTGAGTGGGATTAA

Upstream 100 bases:

>100_bases
CTGTCATTTAATTATTGCTCAATTCGCAAAAATCCTCTGCATTTCTCGCTCTTTTTCTACAAAGCGCTGAAGGTCATAAT
CCCCTCAGTTAACGAACATA

Downstream 100 bases:

>100_bases
TTGCCTGACCCTTTGTACCCTTTACCCCTCTTTCACAGCTCCTTCGGGAGCTGTTCTTATATATTCGCTCAAAAAGTCGA
TAAATGCCCGAATGCGTGTG

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIKKRNSMTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEVAVGQAIAESGVPRNELYITTKIWIENLSKD
KLIPSLKESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGVDNIATNQI
ELSPYLQNRKVVDWAKAHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASNLL
AQDLHLDAEDKNAIAALDCNDRLVSPEGLAPEWD

Sequences:

>Translated_274_residues
MIKKRNSMTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEVAVGQAIAESGVPRNELYITTKIWIENLSKD
KLIPSLKESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGVDNIATNQI
ELSPYLQNRKVVDWAKAHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASNLL
AQDLHLDAEDKNAIAALDCNDRLVSPEGLAPEWD
>Mature_274_residues
MIKKRNSMTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEVAVGQAIAESGVPRNELYITTKIWIENLSKD
KLIPSLKESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGVDNIATNQI
ELSPYLQNRKVVDWAKAHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASNLL
AQDLHLDAEDKNAIAALDCNDRLVSPEGLAPEWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=283, Percent_Identity=31.095406360424, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI45446745, Length=300, Percent_Identity=29.6666666666667, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI4503285, Length=300, Percent_Identity=29.6666666666667, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI310109920, Length=300, Percent_Identity=29.6666666666667, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI5453543, Length=301, Percent_Identity=29.2358803986711, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.2096219931272, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI24497583, Length=305, Percent_Identity=29.5081967213115, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=30.7167235494881, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=30.8771929824561, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4502049, Length=292, Percent_Identity=33.2191780821918, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=30.2238805970149, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=30, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=30.2158273381295, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI310109926, Length=122, Percent_Identity=36.0655737704918, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI207028673, Length=122, Percent_Identity=36.0655737704918, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=28.3870967741935, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=92.5093632958801, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI87082198, Length=265, Percent_Identity=38.8679245283019, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=28.6231884057971, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787674, Length=258, Percent_Identity=28.2945736434109, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994888, Length=238, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17550248, Length=279, Percent_Identity=35.84229390681, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17537075, Length=298, Percent_Identity=35.9060402684564, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537077, Length=296, Percent_Identity=35.8108108108108, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=31.8518518518519, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17552492, Length=269, Percent_Identity=30.8550185873606, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17561298, Length=282, Percent_Identity=30.4964539007092, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17561300, Length=284, Percent_Identity=30.6338028169014, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17538386, Length=290, Percent_Identity=30.6896551724138, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17566692, Length=283, Percent_Identity=31.095406360424, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=26.8707482993197, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=26.7123287671233, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17562292, Length=256, Percent_Identity=29.296875, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324694, Length=289, Percent_Identity=35.2941176470588, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6320576, Length=271, Percent_Identity=35.4243542435424, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321896, Length=313, Percent_Identity=28.7539936102236, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322556, Length=257, Percent_Identity=26.8482490272374, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6319625, Length=271, Percent_Identity=30.2583025830258, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320079, Length=273, Percent_Identity=29.3040293040293, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24644950, Length=301, Percent_Identity=32.890365448505, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24662789, Length=297, Percent_Identity=31.3131313131313, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI221378297, Length=322, Percent_Identity=31.3664596273292, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24657054, Length=292, Percent_Identity=30.4794520547945, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=34.2560553633218, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129731, Length=295, Percent_Identity=30.1694915254237, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45553081, Length=279, Percent_Identity=30.4659498207885, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24662781, Length=297, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24663317, Length=296, Percent_Identity=29.0540540540541, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356425, Length=297, Percent_Identity=29.96632996633, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=27.7992277992278, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 30153; Mature: 30153

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKRNSMTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEVAVGQAIAESG
CCCCCCCEECCEECCEEEEEECCEEEHHHHHHHHHCCHHCCHHHHHCCCHHHHHHHHHCC
VPRNELYITTKIWIENLSKDKLIPSLKESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQA
CCCCCEEEEEEHEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHH
LLEAKKQGLTREIGISNFTIPLMEKAIAAVGVDNIATNQIELSPYLQNRKVVDWAKAHGI
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCEEEHHHHCCE
HITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASNLL
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCHHHHHHHHHHH
AQDLHLDAEDKNAIAALDCNDRLVSPEGLAPEWD
HHHHCCCCCCCCCEEEECCCCCEECCCCCCCCCC
>Mature Secondary Structure
MIKKRNSMTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEVAVGQAIAESG
CCCCCCCEECCEECCEEEEEECCEEEHHHHHHHHHCCHHCCHHHHHCCCHHHHHHHHHCC
VPRNELYITTKIWIENLSKDKLIPSLKESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQA
CCCCCEEEEEEHEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHH
LLEAKKQGLTREIGISNFTIPLMEKAIAAVGVDNIATNQIELSPYLQNRKVVDWAKAHGI
HHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCEEEHHHHCCE
HITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASNLL
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCHHHHHHHHHHH
AQDLHLDAEDKNAIAALDCNDRLVSPEGLAPEWD
HHHHCCCCCCCCCEEEECCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]