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The map label for this gene is (dsbE1

Identifier: 194737651

GI number: 194737651

Start: 2354211

End: 2354768

Strand: Reverse

Name: (dsbE1

Synonym: SeSA_A2474

Alternate gene names: 194737651

Gene position: 2354768-2354211 (Counterclockwise)

Preceding gene: 194737150

Following gene: 194736981

Centisome position: 50.0

GC content: 62.9

Gene sequence:

>558_bases
ATGAAACGCAACGTACTGTTATTACCGCTGCTGATTTTTCTGCTGATTGCCGCGGCGCTGCTGTGGCAGCTGGCGCGCAA
CGCGCAGGGGGATGACCCGACGAATCTCGAATCGGCACTGACCGGAAAGCCGGTGCCGGCGTTCCGCCTGGAATCGCTGG
AGACGCCGGGTCAGTACTACCAGGCGGAGGTGCTGACGCAGGGGAAACCGGTGCTGCTTAACGTCTGGGCCACCTGGTGC
CCGACCTGCCGCGCCGAGCATCAGTACCTGAACCGGCTGGCCGCGCAGGGTATCCGGGTGGTCGGGCTGAACTATAAGGA
CGACCGGGCGAAGGCGGTGGCCTGGTTAAAGGAACTGGGTAACCCGTATGCGCTGAGTTTATCGGACAGCGACGGGATGC
TGGGGCTGGACCTGGGCGTGTACGGCGCGCCGGAAACCTTCCTCATCGACGGCAGGGGGATTATCCGCTACCGCCATGCG
GGCGATTTGAATGCCCGGGTATGGGAAAGTGAACTGAAACCGCTGTGGGACAGATACAGCCGGGAGGCGGCGCAATGA

Upstream 100 bases:

>100_bases
TCCGCTGGATATGGGCCGGGGGACTGCTGATGGCGCTGGGCGGGCTGCTGTGCCTGGCGGACCCGCGCTACCGCCGCCGT
AAACCATTGCCGGAGGCCGG

Downstream 100 bases:

>100_bases
GACTGTTACCGGGCATGGTGATGCTGATGCTGGCGCTGGTTATCTCCGGGTCAGCGCGGGCGACCACCGACGTGATGCCG
TTTAAAGATGAAGCGCAGGA

Product: thiol:disulfide interChange protein DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKRNVLLLPLLIFLLIAAALLWQLARNAQGDDPTNLESALTGKPVPAFRLESLETPGQYYQAEVLTQGKPVLLNVWATWC
PTCRAEHQYLNRLAAQGIRVVGLNYKDDRAKAVAWLKELGNPYALSLSDSDGMLGLDLGVYGAPETFLIDGRGIIRYRHA
GDLNARVWESELKPLWDRYSREAAQ

Sequences:

>Translated_185_residues
MKRNVLLLPLLIFLLIAAALLWQLARNAQGDDPTNLESALTGKPVPAFRLESLETPGQYYQAEVLTQGKPVLLNVWATWC
PTCRAEHQYLNRLAAQGIRVVGLNYKDDRAKAVAWLKELGNPYALSLSDSDGMLGLDLGVYGAPETFLIDGRGIIRYRHA
GDLNARVWESELKPLWDRYSREAAQ
>Mature_185_residues
MKRNVLLLPLLIFLLIAAALLWQLARNAQGDDPTNLESALTGKPVPAFRLESLETPGQYYQAEVLTQGKPVLLNVWATWC
PTCRAEHQYLNRLAAQGIRVVGLNYKDDRAKAVAWLKELGNPYALSLSDSDGMLGLDLGVYGAPETFLIDGRGIIRYRHA
GDLNARVWESELKPLWDRYSREAAQ

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1788523, Length=185, Percent_Identity=85.945945945946, Blast_Score=338, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DSBE_SALTI (Q8XFE5)

Other databases:

- EMBL:   AL627274
- EMBL:   AL627280
- EMBL:   AE014613
- EMBL:   AE014613
- RefSeq:   NP_456793.1
- RefSeq:   NP_458126.1
- RefSeq:   NP_804472.1
- RefSeq:   NP_807340.1
- ProteinModelPortal:   Q8XFE5
- SMR:   Q8XFE5
- GeneID:   1068784
- GeneID:   1069725
- GeneID:   1248802
- GeneID:   1250212
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0616
- KEGG:   stt:t3705
- KEGG:   sty:STY2474
- KEGG:   sty:STY3965
- HOGENOM:   HBG493509
- OMA:   KWLAEFH
- ProtClustDB:   PRK15412
- BioCyc:   SENT209261:T0616-MONOMER
- BioCyc:   SENT209261:T3705-MONOMER
- BioCyc:   SENT220341:STY2474-MONOMER
- BioCyc:   SENT220341:STY3965-MONOMER
- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- TIGRFAMs:   TIGR00385

Pfam domain/function: PF08534 Redoxin; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 20691; Mature: 20691

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x202d0c18)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNVLLLPLLIFLLIAAALLWQLARNAQGDDPTNLESALTGKPVPAFRLESLETPGQYY
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCHHH
QAEVLTQGKPVLLNVWATWCPTCRAEHQYLNRLAAQGIRVVGLNYKDDRAKAVAWLKELG
HHHHHCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCC
NPYALSLSDSDGMLGLDLGVYGAPETFLIDGRGIIRYRHAGDLNARVWESELKPLWDRYS
CCEEEEECCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
REAAQ
HHHCH
>Mature Secondary Structure
MKRNVLLLPLLIFLLIAAALLWQLARNAQGDDPTNLESALTGKPVPAFRLESLETPGQYY
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCHHH
QAEVLTQGKPVLLNVWATWCPTCRAEHQYLNRLAAQGIRVVGLNYKDDRAKAVAWLKELG
HHHHHCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCC
NPYALSLSDSDGMLGLDLGVYGAPETFLIDGRGIIRYRHAGDLNARVWESELKPLWDRYS
CCEEEEECCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
REAAQ
HHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504