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The map label for this gene is queA [H]

Identifier: 194737330

GI number: 194737330

Start: 1592322

End: 1593401

Strand: Direct

Name: queA [H]

Synonym: SeSA_A1653

Alternate gene names: 194737330

Gene position: 1592322-1593401 (Clockwise)

Preceding gene: 194734884

Following gene: 194736573

Centisome position: 33.81

GC content: 38.52

Gene sequence:

>1080_bases
ATGCTTACCGAAAAATATAATTTTCGCATTACCGATCAGATGACTATCCCACTCAGACCTCATTGGATAGCCAATGATTC
ATACAGGGAAAAGTGTAAAATGCTTGTCCTCAACCGGAGTAAAGGGGAAATTCATAAGGTAGACTTTAGTAAAGTAACAG
ATTATATCAAAGAAGGTGATGTAATCTGTTTTAACGACAGTACTATAATAAACCATATGTTTATCTGTAAAACAAGACGG
AATAGATTAATTAAAATTGTTTTAGAGGGATTTTTACCAAATAATAGAGTAATTATTTCCGGACTTTTAAAAGAAAGACT
TAACGCGAATGATGTTTTCTATTTGGTTGATAACCCTGGAATTTCCATTAAAATAGAGCAAAAGTTTAGCGAAGAGAGCC
AGTACCGAGCCGTCGTGGAAAATCATGAGGCCTTAATTTGCTATTTAGCCAGTCATGGTGAGCGTCTTGATGAGTATGTG
GACTCCAGCTTGTTTTATAAATATCCAGATGCTTACCGTTCCGTTTTTTCTAAAAAGTATGGCTCTTTGGAAATACCTTC
TGCCGGCATTCATTTTACCTGGGATCTTATCCAAAGAATCAAAGATAAAGGTGGGTTAATATCTTTTATTACTTTACATG
TTGCATCAACAGAAATGTTGTCCAACAGAAAAATACAAACAAAATGTGTAGAGGAAGTGACTATCAACGAAGAATATTAC
GAGGTTTCGCAAGCAACAGCGGATATCATTAATACTGCGAAGCAAAATGGCGGACGTATTTTTGCCGTAGGAACTACCGT
TACACGATGCCTGGAATCCGCGTATTCGAGGGAGCACAATTGCCTCAAAGCAAGTTCTGGCTGGACTGCACTTTATATAC
ATCCAGGCTATCAACTGAAAGTGGTTGACTGCCTGCTAACGAACTTACATCAGCCAAAAACGACGCATATGGTGTTAACC
GGTCAGTTTGCTGGGGTTGATCTACTCATGAAGGCCTATGCCTCAGAAGATATCCAGTCATGTCAGTTCGATATGTTTGG
TGATTGCATGTTAATCATACAGGATGAAGGGCAGGGATGA

Upstream 100 bases:

>100_bases
ATTTATCGAGTTAATTTTCACCCTTTGAAAGGCTAACGTATACTGCAGAATACGGCTATATAACAATCGCCGTATCAATG
GTTTTTCAAAGGGTAAATAT

Downstream 100 bases:

>100_bases
ATAAAATAAAACCGGTACACATAATGCTACCGGTTTTACCGAGAATAAGCATCAGGGGATTCGGGCAATAACCTTTATTT
CGAAATCAAAGCCGGCCAGC

Product: putative isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLTEKYNFRITDQMTIPLRPHWIANDSYREKCKMLVLNRSKGEIHKVDFSKVTDYIKEGDVICFNDSTIINHMFICKTRR
NRLIKIVLEGFLPNNRVIISGLLKERLNANDVFYLVDNPGISIKIEQKFSEESQYRAVVENHEALICYLASHGERLDEYV
DSSLFYKYPDAYRSVFSKKYGSLEIPSAGIHFTWDLIQRIKDKGGLISFITLHVASTEMLSNRKIQTKCVEEVTINEEYY
EVSQATADIINTAKQNGGRIFAVGTTVTRCLESAYSREHNCLKASSGWTALYIHPGYQLKVVDCLLTNLHQPKTTHMVLT
GQFAGVDLLMKAYASEDIQSCQFDMFGDCMLIIQDEGQG

Sequences:

>Translated_359_residues
MLTEKYNFRITDQMTIPLRPHWIANDSYREKCKMLVLNRSKGEIHKVDFSKVTDYIKEGDVICFNDSTIINHMFICKTRR
NRLIKIVLEGFLPNNRVIISGLLKERLNANDVFYLVDNPGISIKIEQKFSEESQYRAVVENHEALICYLASHGERLDEYV
DSSLFYKYPDAYRSVFSKKYGSLEIPSAGIHFTWDLIQRIKDKGGLISFITLHVASTEMLSNRKIQTKCVEEVTINEEYY
EVSQATADIINTAKQNGGRIFAVGTTVTRCLESAYSREHNCLKASSGWTALYIHPGYQLKVVDCLLTNLHQPKTTHMVLT
GQFAGVDLLMKAYASEDIQSCQFDMFGDCMLIIQDEGQG
>Mature_359_residues
MLTEKYNFRITDQMTIPLRPHWIANDSYREKCKMLVLNRSKGEIHKVDFSKVTDYIKEGDVICFNDSTIINHMFICKTRR
NRLIKIVLEGFLPNNRVIISGLLKERLNANDVFYLVDNPGISIKIEQKFSEESQYRAVVENHEALICYLASHGERLDEYV
DSSLFYKYPDAYRSVFSKKYGSLEIPSAGIHFTWDLIQRIKDKGGLISFITLHVASTEMLSNRKIQTKCVEEVTINEEYY
EVSQATADIINTAKQNGGRIFAVGTTVTRCLESAYSREHNCLKASSGWTALYIHPGYQLKVVDCLLTNLHQPKTTHMVLT
GQFAGVDLLMKAYASEDIQSCQFDMFGDCMLIIQDEGQG

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=335, Percent_Identity=29.2537313432836, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41067; Mature: 41067

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTEKYNFRITDQMTIPLRPHWIANDSYREKCKMLVLNRSKGEIHKVDFSKVTDYIKEGD
CCCCCEEEEEECEEEECCCCCEECCCCHHHHEEEEEEECCCCCEEECCHHHHHHHHHCCC
VICFNDSTIINHMFICKTRRNRLIKIVLEGFLPNNRVIISGLLKERLNANDVFYLVDNPG
EEEECCCHHHHEEEEEEHHCHHEEEEEEECCCCCCEEEEEEHHHHHCCCCCEEEEEECCC
ISIKIEQKFSEESQYRAVVENHEALICYLASHGERLDEYVDSSLFYKYPDAYRSVFSKKY
EEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCEEEECCHHHHHHHHHCC
GSLEIPSAGIHFTWDLIQRIKDKGGLISFITLHVASTEMLSNRKIQTKCVEEVTINEEYY
CCEECCCCCCEEEHHHHHHHHHCCCEEEEEEEEHHHHHHHCCCCHHHHHHHHHCCCHHHH
EVSQATADIINTAKQNGGRIFAVGTTVTRCLESAYSREHNCLKASSGWTALYIHPGYQLK
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCEEE
VVDCLLTNLHQPKTTHMVLTGQFAGVDLLMKAYASEDIQSCQFDMFGDCMLIIQDEGQG
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCHHHHHCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MLTEKYNFRITDQMTIPLRPHWIANDSYREKCKMLVLNRSKGEIHKVDFSKVTDYIKEGD
CCCCCEEEEEECEEEECCCCCEECCCCHHHHEEEEEEECCCCCEEECCHHHHHHHHHCCC
VICFNDSTIINHMFICKTRRNRLIKIVLEGFLPNNRVIISGLLKERLNANDVFYLVDNPG
EEEECCCHHHHEEEEEEHHCHHEEEEEEECCCCCCEEEEEEHHHHHCCCCCEEEEEECCC
ISIKIEQKFSEESQYRAVVENHEALICYLASHGERLDEYVDSSLFYKYPDAYRSVFSKKY
EEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCEEEECCHHHHHHHHHCC
GSLEIPSAGIHFTWDLIQRIKDKGGLISFITLHVASTEMLSNRKIQTKCVEEVTINEEYY
CCEECCCCCCEEEHHHHHHHHHCCCEEEEEEEEHHHHHHHCCCCHHHHHHHHHCCCHHHH
EVSQATADIINTAKQNGGRIFAVGTTVTRCLESAYSREHNCLKASSGWTALYIHPGYQLK
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCEEE
VVDCLLTNLHQPKTTHMVLTGQFAGVDLLMKAYASEDIQSCQFDMFGDCMLIIQDEGQG
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCHHHHHCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA