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The map label for this gene is holB [H]

Identifier: 194737080

GI number: 194737080

Start: 1248909

End: 1249913

Strand: Direct

Name: holB [H]

Synonym: SeSA_A1277

Alternate gene names: 194737080

Gene position: 1248909-1249913 (Clockwise)

Preceding gene: 194733912

Following gene: 194734591

Centisome position: 26.52

GC content: 58.51

Gene sequence:

>1005_bases
ATGAAATGGTATCCATGGTTACGGCCCGCCTATGAAAAACTGGTAGAAAGTTACCAGGCGGGACGTGGGCACCATGCGCT
ACTGATTCAGGCCTTACCTGGCATGGGCGACGAGGCGCTGTGCTATGCGCTGAGCCGTTATCTGTTATGCCAGCAGCCTG
AGGGCCACAAAAGCTGTGGGCATTGCCGTGGCTGTCAGCTTATGCAGGCTGGCACGCATCCGGACTATTACACGCTGACG
CCGGATAAAGGCAAAAGCAGCCTCGGCGTAGATGCGGTACGCGAGGTCAGCGAGAAATTGTACGAGCACTCCCGTCTGGG
CGGTGCGAAGGTGGTGTGGATTGCGGATGCGGCGCTATTAACCGACGCCGCTGCCAATGCGTTGCTCAAAACGCTGGAAG
AGCCGCCGGAGCAAACCTGGTTTTTCCTTGCTAGCCCGGAGCCTGCTCGTCTGTTGGCGACATTACGCAGCCGCTGCCGG
CTGCATCATCTGGCGCCGCCGTCCGAATCGTATGCGATGTCATGGCTGTCGCGAGAAGTGACGGCGTCACAAGAAGCGTT
GCTCACGGCGCTACGCCTGAATGCGGGGTCGCCCGGCGCCGCGCTGGCGCTGTTGCAGTCTGAACGTTGGGCGCAGCGTG
AGGCGCTGTGTCAGGCGTTAATGGACAGTTTACATACGGGCGACTGGTATGCGTTATTAACGGCGCTCAATCATGAGCAA
GCCCCGGCGCGCCTGCACTGGCTGGCGACGCTGCTGGTTGATGCTCTTAAACGTCAGCACGGCGCGTCGTACCTGACAAA
CGTTGATGCGGACGCGGTTGTTGCCGCGTTGGCGGGGCCGCTATCCCCGGCGCGTATTCAGGCAATCCTGAACGATGTTT
GCCATTGTCGTGATCAATTACTTCATGTGACCGGATTAAACCGCGAACTGGTATTAACCGATCTCATTCTCCGCATCGAG
CATTACCTGCAACCAGGCACCTTGTTGCCTGTTCCTCATCTTTGA

Upstream 100 bases:

>100_bases
AAGACTCGCGTATCCGCACCATTGACGCGACCCAGCCGCTGGATGCCGTCATGCGCGACATTCGCGCCACGGTGACGAAG
TGGGTACAGGAGCAAGCGGC

Downstream 100 bases:

>100_bases
GAGAGACATTATGTTTTTAGTCGACTCACACTGCCATCTTGATGGTCTGGATTATCAATCTTTGCATAAGGACGTGGACG
ATGTGCTGGCGAAAGCCGCC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKWYPWLRPAYEKLVESYQAGRGHHALLIQALPGMGDEALCYALSRYLLCQQPEGHKSCGHCRGCQLMQAGTHPDYYTLT
PDKGKSSLGVDAVREVSEKLYEHSRLGGAKVVWIADAALLTDAAANALLKTLEEPPEQTWFFLASPEPARLLATLRSRCR
LHHLAPPSESYAMSWLSREVTASQEALLTALRLNAGSPGAALALLQSERWAQREALCQALMDSLHTGDWYALLTALNHEQ
APARLHWLATLLVDALKRQHGASYLTNVDADAVVAALAGPLSPARIQAILNDVCHCRDQLLHVTGLNRELVLTDLILRIE
HYLQPGTLLPVPHL

Sequences:

>Translated_334_residues
MKWYPWLRPAYEKLVESYQAGRGHHALLIQALPGMGDEALCYALSRYLLCQQPEGHKSCGHCRGCQLMQAGTHPDYYTLT
PDKGKSSLGVDAVREVSEKLYEHSRLGGAKVVWIADAALLTDAAANALLKTLEEPPEQTWFFLASPEPARLLATLRSRCR
LHHLAPPSESYAMSWLSREVTASQEALLTALRLNAGSPGAALALLQSERWAQREALCQALMDSLHTGDWYALLTALNHEQ
APARLHWLATLLVDALKRQHGASYLTNVDADAVVAALAGPLSPARIQAILNDVCHCRDQLLHVTGLNRELVLTDLILRIE
HYLQPGTLLPVPHL
>Mature_334_residues
MKWYPWLRPAYEKLVESYQAGRGHHALLIQALPGMGDEALCYALSRYLLCQQPEGHKSCGHCRGCQLMQAGTHPDYYTLT
PDKGKSSLGVDAVREVSEKLYEHSRLGGAKVVWIADAALLTDAAANALLKTLEEPPEQTWFFLASPEPARLLATLRSRCR
LHHLAPPSESYAMSWLSREVTASQEALLTALRLNAGSPGAALALLQSERWAQREALCQALMDSLHTGDWYALLTALNHEQ
APARLHWLATLLVDALKRQHGASYLTNVDADAVVAALAGPLSPARIQAILNDVCHCRDQLLHVTGLNRELVLTDLILRIE
HYLQPGTLLPVPHL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=334, Percent_Identity=79.3413173652695, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1786676, Length=144, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36858; Mature: 36858

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWYPWLRPAYEKLVESYQAGRGHHALLIQALPGMGDEALCYALSRYLLCQQPEGHKSCG
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCC
HCRGCQLMQAGTHPDYYTLTPDKGKSSLGVDAVREVSEKLYEHSRLGGAKVVWIADAALL
CCCCCHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
TDAAANALLKTLEEPPEQTWFFLASPEPARLLATLRSRCRLHHLAPPSESYAMSWLSREV
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
TASQEALLTALRLNAGSPGAALALLQSERWAQREALCQALMDSLHTGDWYALLTALNHEQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
APARLHWLATLLVDALKRQHGASYLTNVDADAVVAALAGPLSPARIQAILNDVCHCRDQL
CHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LHVTGLNRELVLTDLILRIEHYLQPGTLLPVPHL
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKWYPWLRPAYEKLVESYQAGRGHHALLIQALPGMGDEALCYALSRYLLCQQPEGHKSCG
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCC
HCRGCQLMQAGTHPDYYTLTPDKGKSSLGVDAVREVSEKLYEHSRLGGAKVVWIADAALL
CCCCCHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
TDAAANALLKTLEEPPEQTWFFLASPEPARLLATLRSRCRLHHLAPPSESYAMSWLSREV
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
TASQEALLTALRLNAGSPGAALALLQSERWAQREALCQALMDSLHTGDWYALLTALNHEQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
APARLHWLATLLVDALKRQHGASYLTNVDADAVVAALAGPLSPARIQAILNDVCHCRDQL
CHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LHVTGLNRELVLTDLILRIEHYLQPGTLLPVPHL
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 8509334; 8905232; 9278503; 8505304; 1575709; 9363942 [H]