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The map label for this gene is puuB [H]

Identifier: 194736502

GI number: 194736502

Start: 3187504

End: 3188805

Strand: Reverse

Name: puuB [H]

Synonym: SeSA_A3304

Alternate gene names: 194736502

Gene position: 3188805-3187504 (Counterclockwise)

Preceding gene: 194734634

Following gene: 194738392

Centisome position: 67.72

GC content: 49.0

Gene sequence:

>1302_bases
ATGAATACAAAAATCGATGCGTTGAATTACTACGGCGCGACGAAAAAATACCCGTTGCATTTTCCTGCTTTGCAAGAGGA
TATTGATGCCGATGTGGTGATCATTGGCGGTGGCTTTTCAGGCATCAATACCGCGCTGGAACTGGCGGAGCAGGGGATCA
CTAATGTTGTCGTTCTGGAAGCCCGTCATCTTGGGTATGGCGGCACCGGACGAAACGGCGGTCAGGTAATGGCCGGTATA
GGTCACGACATCGAAGCCGTTAAAAAATATGTAGGCGCAGCGGGAATGGAGACGCTGTTTAAAATCTCGAACCTCGGCGC
GGGTATTATTCGGGAGCGAATTCGTAAATACCATATTGATGCTGATTTTGTCCCCGGATATGGTTATCTGGCCTACAACC
AGCGGCAGTTAAAAACGCTGCAACAATGGGAAAAAGAATTCAAAGCCGCTACGCCGGAAGACGATATTGAACTCTATACC
GGTAAAGACGTGCAGCAGGTTATCGGTTCCGACGTCTACTGCGGTGTTCTCAAGCATATGGGGGGAGGGCATGTCCACTC
GCTGAATTTATTGCTGGGCTCCGCTCAGGCTGCGAATTCGCTGGGGGTGAAGATATTCGAATATTCACCTGTGGTTGAGG
TCAGTTACGGTAAAACTGTGCGGGTACGTACTGCGATGGGCAGCGTAAAAGCAGCCAAATTGCTGTGGGCCTGTGATAGT
TTTCTCAATAATCTGGAACCAGAAATTTATAAGAAAACGCTGGTTACTTATTCTTATCAGGTCTCTACAGAACCTCTCTC
CAGCGAGCTTGTTGAGCGGATCAGTCCCTTGCGCGGGGCTTTTAGCGATATCCGCCCGGTGATAAATTACTACCGTCTCA
CAAAAGAAAACCGTCTGCTGTTTGGCAGCGCCACAAGGTTTCTTGAATATACCCCGCACGATTTTGCTGCCTGGAACCGC
ACTCTGTTGACGGAGGTCTTCCCTTATTTGAAAGACGTTAAAATCGATTTTGCCTGGGGCGGACCGATGGCCTGTAGCGC
GAATCTGTTTCCGCAGATTGGCACGCTTCGTGAACACAATAATGTTTTTTATGTTCAGGGTTATTCCGGATTTGGCGTCA
CGCCTTCCCATATTGTATGCAAAATCCTGGCCGAAGGAATAAATGGCGGTTCCGAGCGGTATCGTCTGATGAGTAGCATT
CCCCACGCGACAATTTATGGTCGCGACAGTTTACGTCTATTGCTGGTCAGTGCGGGAAAACTGATACATCAAACAGCCGG
TTTCTGGAAAGGCCGGAATTAA

Upstream 100 bases:

>100_bases
AATATCGTTCCAAAGAACGTCATTCTTCCGGACGATATTTTGTAAAACGTTACCGTGCTGTGATAGCGCTCTTAGTCAAG
CGTGATGACAAGGTAACGCG

Downstream 100 bases:

>100_bases
CCAGGAGAACATTAATGCACGCTTTTAAACTCAATCAACCTGTTCCTGAATTACAGCCAGTCGGGAGTGTCAGCCTGTTA
GGGGCGTTGCCAACAGAAGG

Product: tyramine oxidase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNTKIDALNYYGATKKYPLHFPALQEDIDADVVIIGGGFSGINTALELAEQGITNVVVLEARHLGYGGTGRNGGQVMAGI
GHDIEAVKKYVGAAGMETLFKISNLGAGIIRERIRKYHIDADFVPGYGYLAYNQRQLKTLQQWEKEFKAATPEDDIELYT
GKDVQQVIGSDVYCGVLKHMGGGHVHSLNLLLGSAQAANSLGVKIFEYSPVVEVSYGKTVRVRTAMGSVKAAKLLWACDS
FLNNLEPEIYKKTLVTYSYQVSTEPLSSELVERISPLRGAFSDIRPVINYYRLTKENRLLFGSATRFLEYTPHDFAAWNR
TLLTEVFPYLKDVKIDFAWGGPMACSANLFPQIGTLREHNNVFYVQGYSGFGVTPSHIVCKILAEGINGGSERYRLMSSI
PHATIYGRDSLRLLLVSAGKLIHQTAGFWKGRN

Sequences:

>Translated_433_residues
MNTKIDALNYYGATKKYPLHFPALQEDIDADVVIIGGGFSGINTALELAEQGITNVVVLEARHLGYGGTGRNGGQVMAGI
GHDIEAVKKYVGAAGMETLFKISNLGAGIIRERIRKYHIDADFVPGYGYLAYNQRQLKTLQQWEKEFKAATPEDDIELYT
GKDVQQVIGSDVYCGVLKHMGGGHVHSLNLLLGSAQAANSLGVKIFEYSPVVEVSYGKTVRVRTAMGSVKAAKLLWACDS
FLNNLEPEIYKKTLVTYSYQVSTEPLSSELVERISPLRGAFSDIRPVINYYRLTKENRLLFGSATRFLEYTPHDFAAWNR
TLLTEVFPYLKDVKIDFAWGGPMACSANLFPQIGTLREHNNVFYVQGYSGFGVTPSHIVCKILAEGINGGSERYRLMSSI
PHATIYGRDSLRLLLVSAGKLIHQTAGFWKGRN
>Mature_433_residues
MNTKIDALNYYGATKKYPLHFPALQEDIDADVVIIGGGFSGINTALELAEQGITNVVVLEARHLGYGGTGRNGGQVMAGI
GHDIEAVKKYVGAAGMETLFKISNLGAGIIRERIRKYHIDADFVPGYGYLAYNQRQLKTLQQWEKEFKAATPEDDIELYT
GKDVQQVIGSDVYCGVLKHMGGGHVHSLNLLLGSAQAANSLGVKIFEYSPVVEVSYGKTVRVRTAMGSVKAAKLLWACDS
FLNNLEPEIYKKTLVTYSYQVSTEPLSSELVERISPLRGAFSDIRPVINYYRLTKENRLLFGSATRFLEYTPHDFAAWNR
TLLTEVFPYLKDVKIDFAWGGPMACSANLFPQIGTLREHNNVFYVQGYSGFGVTPSHIVCKILAEGINGGSERYRLMSSI
PHATIYGRDSLRLLLVSAGKLIHQTAGFWKGRN

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=412, Percent_Identity=36.1650485436893, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47867; Mature: 47867

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKIDALNYYGATKKYPLHFPALQEDIDADVVIIGGGFSGINTALELAEQGITNVVVLE
CCCCCCCEEECCCCCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEE
ARHLGYGGTGRNGGQVMAGIGHDIEAVKKYVGAAGMETLFKISNLGAGIIRERIRKYHID
EECCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
ADFVPGYGYLAYNQRQLKTLQQWEKEFKAATPEDDIELYTGKDVQQVIGSDVYCGVLKHM
CCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHC
GGGHVHSLNLLLGSAQAANSLGVKIFEYSPVVEVSYGKTVRVRTAMGSVKAAKLLWACDS
CCCCEEEEEEEEECCCCCCCCCEEEEEECCEEEEECCCEEEEEEHHCCHHHHHHHHHHHH
FLNNLEPEIYKKTLVTYSYQVSTEPLSSELVERISPLRGAFSDIRPVINYYRLTKENRLL
HHHCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FGSATRFLEYTPHDFAAWNRTLLTEVFPYLKDVKIDFAWGGPMACSANLFPQIGTLREHN
EECHHHHEECCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEECCCCCCCCCCCEECC
NVFYVQGYSGFGVTPSHIVCKILAEGINGGSERYRLMSSIPHATIYGRDSLRLLLVSAGK
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEEEEEEHHHH
LIHQTAGFWKGRN
HHHHHHCCCCCCC
>Mature Secondary Structure
MNTKIDALNYYGATKKYPLHFPALQEDIDADVVIIGGGFSGINTALELAEQGITNVVVLE
CCCCCCCEEECCCCCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEE
ARHLGYGGTGRNGGQVMAGIGHDIEAVKKYVGAAGMETLFKISNLGAGIIRERIRKYHID
EECCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
ADFVPGYGYLAYNQRQLKTLQQWEKEFKAATPEDDIELYTGKDVQQVIGSDVYCGVLKHM
CCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHC
GGGHVHSLNLLLGSAQAANSLGVKIFEYSPVVEVSYGKTVRVRTAMGSVKAAKLLWACDS
CCCCEEEEEEEEECCCCCCCCCEEEEEECCEEEEECCCEEEEEEHHCCHHHHHHHHHHHH
FLNNLEPEIYKKTLVTYSYQVSTEPLSSELVERISPLRGAFSDIRPVINYYRLTKENRLL
HHHCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FGSATRFLEYTPHDFAAWNRTLLTEVFPYLKDVKIDFAWGGPMACSANLFPQIGTLREHN
EECHHHHEECCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEECCCCCCCCCCCEECC
NVFYVQGYSGFGVTPSHIVCKILAEGINGGSERYRLMSSIPHATIYGRDSLRLLLVSAGK
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEEEEEEHHHH
LIHQTAGFWKGRN
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]