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The map label for this gene is spoT [H]

Identifier: 194736380

GI number: 194736380

Start: 3819232

End: 3821343

Strand: Direct

Name: spoT [H]

Synonym: SeSA_A3950

Alternate gene names: 194736380

Gene position: 3819232-3821343 (Clockwise)

Preceding gene: 194737850

Following gene: 194735347

Centisome position: 81.1

GC content: 53.98

Gene sequence:

>2112_bases
TTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAGATTAAGCGTCTTCGGCAGGCGTA
TCTCGTTGCACGTGACGCTCACGAGGGCCAGACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTGGCCT
GTATTCTGGCCGAGATGAAACTCGACTACGAAACGCTGATGGCCGCTCTGCTGCATGACGTGATTGAAGATACCCCCGCC
ACCTATCAGGACATGGAACAGCTTTTCGGTAAAAGCGTTGCCGAGCTGGTAGAGGGGGTATCGAAACTTGATAAGCTCAA
GTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTCCGCAAAATGATTATGGCGATGGTGCAGGATATCCGCGTCATCC
TCATTAAGCTTGCTGACCGTACCCATAACATGCGCACGCTGGGCTCGTTACGCCCGGACAAACGTCGCCGCATCGCCCGT
GAAACCCTCGAAATTTACAGCCCGCTGGCACACCGTTTAGGTATCCATCACATCAAGACCGAGCTTGAAGAGCTGGGTTT
TGAAGCGCTGTATCCTAACCGCTACCGCGTCATCAAAGAAGTGGTAAAAGCGGCGCGCGGCAACCGTAAAGAGATGATCC
AAAAAATCCTCTCTGAAATCGAAGGACGTTTGCAAGAGGCGGGCATTCCGTGTCGCGTTAGCGGTCGCGAAAAACATCTT
TACTCGATCTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATTTACGCTTTCCGCGTCATCGT
TCATGACTCCGATACCTGCTATCGCGTACTCGGCCAGATGCACAGTCTCTATAAGCCTCGTCCGGGACGGGTGAAAGACT
ATATTGCCATTCCCAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGCGTTCCTGTTGAAGTC
CAGATCCGTACCGAAGATATGGATCAGATGGCGGAAATGGGGGTCGCGGCGCACTGGGCGTATAAAGAACACGGTGAGAC
CAGCACCACGGCGCAGATCCGCGCCCAGCGCTGGATGCAGAGCCTGCTGGAGCTACAACAGAGCGCCGGTAGTTCGTTTG
AATTTATCGAAAGCGTAAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACCCCGGAAGGACGCATTGTCGAACTG
CCCGCTGGCGCTACGCCGGTGGACTTCGCCTACGCCGTGCATACCGATATCGGCCACGCCTGCGTCGGCGCGCGTGTCGA
CCGGCAGCCTTATCCGCTGTCGCAGCCGCTTAGCAGCGGTCAGACCGTCGAAATTATTACCGCGCCGGGCGCGCGTCCCA
ACGCCGCCTGGCTGAACTTTGTCGTCAGCTCTAAAGCGCGCGCTAAAATTCGTCAGTTGCTGAAAAACCTCAAACGTGAT
GACTCCGTAAGCCTGGGCCGTCGTCTGCTTAACCATGCTTTAGGCGGTAGTCGTAAGCTGGCGGAAATTCCGCAGGAAAA
TATTCAGCGCGAATTGGATCGTATGAAGCTGGCAACGCTTGACGATCTGCTGGCGGAAATCGGTCTCGGCAACGCGATGA
GCGTAGTGGTCGCGAAAAATCTGCAGCAAGGCGAAGCCGTGGTGCCGACCGTTGCGCAATCGAATCACGGCCACCTGCCG
ATTAAAGGCGCGGATGGCGTGCTTATCACCTTTGCGAAGTGTTGTCGTCCGATCCCAGGCGACCCGATCATCGCTCACGT
CAGCCCAGGTAAAGGACTGGTGATCCACCATGAATCCTGCCGTAATATCCGTGGATACCAGAAAGAGCCAGAGAAATTTA
TGGCGGTCGAATGGGACAAAGAGACGGAGCAGGAATTCATTACCGAAATCAAGGTGGAAATGTTTAACCATCAGGGCGCG
CTGGCTAACCTGACGGCGGCGATTAATACCACCACCTCCAATATTCAAAGCCTGAATACTGAAGAGAAAGATGGTCGCGT
CTATAGTACCTTTATTCGCCTTACCGCACGCGATCGCGTACATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAG
ACGTTATTAAAGTCACCCGTAACCGAAACTAG

Upstream 100 bases:

>100_bases
CGTCCGCGAGCGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTT
AATCACAAAGCGGGTCGCCC

Downstream 100 bases:

>100_bases
CGCTATGAATCCAAAACGTTATGCGCGTATATGCGAAATGCTCGCCAGGCGTCAGCCTGATCTCACGGTCTGCATGGAGC
AAGTCCACAAACCTCATAAC

Product: bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
DSVSLGRRLLNHALGGSRKLAEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQQGEAVVPTVAQSNHGHLP
IKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETEQEFITEIKVEMFNHQGA
LANLTAAINTTTSNIQSLNTEEKDGRVYSTFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN

Sequences:

>Translated_703_residues
MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
DSVSLGRRLLNHALGGSRKLAEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQQGEAVVPTVAQSNHGHLP
IKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETEQEFITEIKVEMFNHQGA
LANLTAAINTTTSNIQSLNTEEKDGRVYSTFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
>Mature_703_residues
MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMKLDYETLMAALLHDVIEDTPA
TYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEV
QIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVEL
PAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
DSVSLGRRLLNHALGGSRKLAEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQQGEAVVPTVAQSNHGHLP
IKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETEQEFITEIKVEMFNHQGA
LANLTAAINTTTSNIQSLNTEEKDGRVYSTFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=703, Percent_Identity=98.1507823613087, Blast_Score=1432, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=32.2108345534407, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25143118, Length=139, Percent_Identity=33.0935251798561, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650996, Length=118, Percent_Identity=40.6779661016949, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79517; Mature: 79517

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHH
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
HHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCHHHEEECCCCCHH
CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DSVSLGRRLLNHALGGSRKLAEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKN
CHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
LQQGEAVVPTVAQSNHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESC
CCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEECHHH
RNIRGYQKEPEKFMAVEWDKETEQEFITEIKVEMFNHQGALANLTAAINTTTSNIQSLNT
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCC
EEKDGRVYSTFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
CCCCCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEPYITHPVAVACILAEMK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
LDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSKLDKLKFRDKKEAQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIARETLEIYSPLAHRLGIHHIKT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILSEIEGRLQEAGIPCRVSGREKHL
HHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
YSIYCKMVLKEQRFHSIMDIYAFRVIVHDSDTCYRVLGQMHSLYKPRPGRVKDYIAIPKA
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMGVAAHWAYKEHGETSTTAQIRAQRWMQ
CCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHH
SLLELQQSAGSSFEFIESVKSDLFPDEIYVFTPEGRIVELPAGATPVDFAYAVHTDIGHA
HHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCHHHEEECCCCCHH
CVGARVDRQPYPLSQPLSSGQTVEIITAPGARPNAAWLNFVVSSKARAKIRQLLKNLKRD
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DSVSLGRRLLNHALGGSRKLAEIPQENIQRELDRMKLATLDDLLAEIGLGNAMSVVVAKN
CHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
LQQGEAVVPTVAQSNHGHLPIKGADGVLITFAKCCRPIPGDPIIAHVSPGKGLVIHHESC
CCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEECHHH
RNIRGYQKEPEKFMAVEWDKETEQEFITEIKVEMFNHQGALANLTAAINTTTSNIQSLNT
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHCCC
EEKDGRVYSTFIRLTARDRVHLANIMRKIRVMPDVIKVTRNRN
CCCCCHHEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]