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The map label for this gene is apbE [H]

Identifier: 194735324

GI number: 194735324

Start: 2370054

End: 2371106

Strand: Reverse

Name: apbE [H]

Synonym: SeSA_A2492

Alternate gene names: 194735324

Gene position: 2371106-2370054 (Counterclockwise)

Preceding gene: 194736918

Following gene: 194735664

Centisome position: 50.35

GC content: 56.98

Gene sequence:

>1053_bases
ATGAAAATGACTTTTTGCCGGGCCGTGTGTCTGGCGGCGGCTTTTTTACTTATGGGCTGCGATGAGGCTCCCGAAACGAC
AACAGCGTCACCTGCCGCTCAGGTGCTGGAAGGTAAGACGATGGGGACTATCTGGCGGGTGAGCGTGGTTGGCATCGATG
CGAAACGCGCCGCAGAGCTACAGACTAAAATCCAGACTCAGCTTGATGCTGATGATTGGTTGCTTTCTACCTATAAAAAT
GACTCCGCGCTGATGCGTTTTAACCATTCACGCAGTCTTGCGCCCTGGCCGGTCAGCGAAGCTATGGCGGATATCGTGAC
CTCGGCGCTGCGTATTGGCGCGAAGACGGATGGCGCGATGGATATCACCGTGGGCCCGCTGGTCAATCTGTGGGGGTTTG
GACCGGATCGGCAGCCGCTGCATATCCCAACACCAGCACAAATCGATGCGGCAAAAGCGAAAACAGGCCTGCAACACTTG
CAGGTTATCGACAGGGCTGGGCATCAGTTTTTGCAAAAAGATCTGCCGGATCTTTATGTTGATCTCTCCACGGTCGGGGA
AGGCTATGCGGCGGATCATCTGGCGCGTCTGATGGAGCAGGAGGGCATTGCGCGTTATCTGGTCTCGGTGGGCGGCGCAT
TAAGCAGTCGCGGGATGAATGCGCAAGGGCTGCCGTGGCGCGTCGCGATTCAGAAGCCGACCGACCGGGAAAACGCGGTG
CAGGCGATTGTGGATATCAACGGGCATGGCATCAGCACCTCCGGCAGCTACCGTAACTATTATGAGCTGGATGGCAAGCG
TGTCTCGCACGTTATCGATCCGCAAACGGGGCGCCCCATTGAACACAACCTGGTATCGGTTACGGTCATCGCGCCAACGG
CGCTGGAAGCGGACGGCTGGGACACCGGCCTGATGGTGCTCGGTACGCAAAAGGCGCAAGAGGTCGTGCGGCGGGAAGGG
CTGGCGGTCTTTATGATCATGAAAGAAGGTGAGGGCTTTAAAACCTGGATGTCGCCGCAGTTCAAAACGTTCCTGGTGAG
CGATAAGAATTAA

Upstream 100 bases:

>100_bases
TGCTAAAGGGCCTGCGGGCCCTTTTTTCATGCCTTATTCCGGCGTACAAATACGACGTTTTGATATACGCTTGCGTCCAT
TCGAATGAGGATAAACAGCG

Downstream 100 bases:

>100_bases
AACGCAAGATTACGGGTTTTTGTGGGCGGATTTACGGACACACTGCATGTAAGCAAGCACTATGCTTAATGAAGGAGTCT
ATGATGAAAAAAGCGTTACT

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTIWRVSVVGIDAKRAAELQTKIQTQLDADDWLLSTYKN
DSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAMDITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHL
QVIDRAGHQFLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGLPWRVAIQKPTDRENAV
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGWDTGLMVLGTQKAQEVVRREG
LAVFMIMKEGEGFKTWMSPQFKTFLVSDKN

Sequences:

>Translated_350_residues
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTIWRVSVVGIDAKRAAELQTKIQTQLDADDWLLSTYKN
DSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAMDITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHL
QVIDRAGHQFLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGLPWRVAIQKPTDRENAV
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGWDTGLMVLGTQKAQEVVRREG
LAVFMIMKEGEGFKTWMSPQFKTFLVSDKN
>Mature_350_residues
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTIWRVSVVGIDAKRAAELQTKIQTQLDADDWLLSTYKN
DSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAMDITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHL
QVIDRAGHQFLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGLPWRVAIQKPTDRENAV
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGWDTGLMVLGTQKAQEVVRREG
LAVFMIMKEGEGFKTWMSPQFKTFLVSDKN

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=351, Percent_Identity=80.3418803418803, Blast_Score=595, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38134; Mature: 38134

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTIWRVSVVGIDAKRAAEL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEECCHHHHHHH
QTKIQTQLDADDWLLSTYKNDSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAM
HHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
DITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHLQVIDRAGHQFLQKDLPDLYV
EEEHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
DLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGLPWRVAIQKPTDRENAV
EHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCCCHHHHE
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGW
EEEEEECCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCCCCCEEEEEEEECCEECCCCC
DTGLMVLGTQKAQEVVRREGLAVFMIMKEGEGFKTWMSPQFKTFLVSDKN
CCCEEEEECHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCEEEEEEECCC
>Mature Secondary Structure
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTIWRVSVVGIDAKRAAEL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEECCHHHHHHH
QTKIQTQLDADDWLLSTYKNDSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAM
HHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
DITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHLQVIDRAGHQFLQKDLPDLYV
EEEHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
DLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGLPWRVAIQKPTDRENAV
EHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCCCHHHHE
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGW
EEEEEECCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCCCCCEEEEEEEECCEECCCCC
DTGLMVLGTQKAQEVVRREGLAVFMIMKEGEGFKTWMSPQFKTFLVSDKN
CCCEEEEECHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9473043; 11677609; 1904855 [H]