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The map label for this gene is atsB [H]

Identifier: 194735302

GI number: 194735302

Start: 3182388

End: 3183572

Strand: Reverse

Name: atsB [H]

Synonym: SeSA_A3298

Alternate gene names: 194735302

Gene position: 3183572-3182388 (Counterclockwise)

Preceding gene: 194734005

Following gene: 194735305

Centisome position: 67.61

GC content: 49.79

Gene sequence:

>1185_bases
ATGTTGAATCTCAACACATTACGGCAACAACAAATCCCCGTAATGACTGAATATCGCGCGCAGATACCCTTTCATATTCT
GGCAAAACCCATAGGCCCTGCGTGTAATCTGGCCTGCCGCTATTGTTATTACCCACAGGGCGAAACGCCCGTAGAAAAAA
TGAATGAATCAACGCTGGAGGTTTTTATTTGTCGCTATATTGCGGCGCAACCTGCCAGTGCGCGTGAAATTAATTTTGTC
TGGCAAGGCGGTGAACCGCTTTTAGCCGGAATCGGTTTTTATAAAAAGGTAATAGCGCTTCAACAACGATATGCGCCTGA
CGACGTGACGATCAGTAATAGTCTGCAAACGAATGCGACGTTGTTAAACGATGCCTGGTGTCGTCTGTTTCGCGACAATA
ATTTTACTATTGGCATCAGTCTTGAGGGCAGTGAAGACTTGCAAAATTACCATCGTCCGGGCAAACGCGGCGAGTCCAGT
TATCCGGCGGTGTTGCGGGGAATCACATTGTTACAACACTATCGAGTCGATTTTAACGTACTGATTGTCGTGCATGATGA
CATGGCTCGCCATGCGGCAGCCATCTACGATCATGTTGTTAGCCTTGGCGCTCGTTATCTGCAATTTCAGCCACTGATGA
ACGAAGGCAACGCCCTACAGCAACGTTACCAATTGAGTGCGGATAACTGGGGACGTTTCATGATTGATATCTGGCGTCAA
TGGCGCAAACGCGGCGATATGGGGCGGGTCTTTGTGATCAACATTGAACAGGCATGGGCACAATATTTTACGCATATCAG
CGCCACCTGTGTCCATTCCGCCCGCTGCGGCACGAATCTGGTCATGGAGCCGGACGGCAAACTCTATGCCTGCGATCATC
TGATTAATAGCCAGCATTACCTGGGACAGCTTTCTAATAATATGTTAGCGCCAGCCGTAGATACCGCAACCCGGCTTCCC
TTTGGTATTAAGAAAAGCCAGCGCCGGGAGTGTCAACGGTGTTCTGTGAAAATAGTCTGCCAGGGAGGCTGCCCCGCACA
TATCAACAGTGCCGGCTACAACCGACTTTGTAGCGGCTATTACTCTTTTTTCACTGAGATTCTGGCTCCGCTACGCGCCT
GGCCCCGGAATCTGAATGGACTGAAAGCCTGGCGTGCTGACGTTATGGGCAGATTTTCGGGCTGA

Upstream 100 bases:

>100_bases
ACAGCCCGCTTTCGGCATTCTGCCCGCAGCACCAGACGGACGCTATTGCGAAATGTGAGTCATGTCTCCGCATCAGAAAT
GATAATTACAGGACAGAATA

Downstream 100 bases:

>100_bases
TGGTCCATCTTGCCGCATTTGCCCGCCGCTCTTGTACAACATCCGTAGTCTGAAATGGAGAATAAGCCGTGAAAAAACAA
GTAACACTTGCCACACTCAG

Product: arylsulfatase-activating protein AtsB

Products: NA

Alternate protein names: AnSME; Arylsulfatase-activating protein; Ser-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLNLNTLRQQQIPVMTEYRAQIPFHILAKPIGPACNLACRYCYYPQGETPVEKMNESTLEVFICRYIAAQPASAREINFV
WQGGEPLLAGIGFYKKVIALQQRYAPDDVTISNSLQTNATLLNDAWCRLFRDNNFTIGISLEGSEDLQNYHRPGKRGESS
YPAVLRGITLLQHYRVDFNVLIVVHDDMARHAAAIYDHVVSLGARYLQFQPLMNEGNALQQRYQLSADNWGRFMIDIWRQ
WRKRGDMGRVFVINIEQAWAQYFTHISATCVHSARCGTNLVMEPDGKLYACDHLINSQHYLGQLSNNMLAPAVDTATRLP
FGIKKSQRRECQRCSVKIVCQGGCPAHINSAGYNRLCSGYYSFFTEILAPLRAWPRNLNGLKAWRADVMGRFSG

Sequences:

>Translated_394_residues
MLNLNTLRQQQIPVMTEYRAQIPFHILAKPIGPACNLACRYCYYPQGETPVEKMNESTLEVFICRYIAAQPASAREINFV
WQGGEPLLAGIGFYKKVIALQQRYAPDDVTISNSLQTNATLLNDAWCRLFRDNNFTIGISLEGSEDLQNYHRPGKRGESS
YPAVLRGITLLQHYRVDFNVLIVVHDDMARHAAAIYDHVVSLGARYLQFQPLMNEGNALQQRYQLSADNWGRFMIDIWRQ
WRKRGDMGRVFVINIEQAWAQYFTHISATCVHSARCGTNLVMEPDGKLYACDHLINSQHYLGQLSNNMLAPAVDTATRLP
FGIKKSQRRECQRCSVKIVCQGGCPAHINSAGYNRLCSGYYSFFTEILAPLRAWPRNLNGLKAWRADVMGRFSG
>Mature_394_residues
MLNLNTLRQQQIPVMTEYRAQIPFHILAKPIGPACNLACRYCYYPQGETPVEKMNESTLEVFICRYIAAQPASAREINFV
WQGGEPLLAGIGFYKKVIALQQRYAPDDVTISNSLQTNATLLNDAWCRLFRDNNFTIGISLEGSEDLQNYHRPGKRGESS
YPAVLRGITLLQHYRVDFNVLIVVHDDMARHAAAIYDHVVSLGARYLQFQPLMNEGNALQQRYQLSADNWGRFMIDIWRQ
WRKRGDMGRVFVINIEQAWAQYFTHISATCVHSARCGTNLVMEPDGKLYACDHLINSQHYLGQLSNNMLAPAVDTATRLP
FGIKKSQRRECQRCSVKIVCQGGCPAHINSAGYNRLCSGYYSFFTEILAPLRAWPRNLNGLKAWRADVMGRFSG

Specific function: Involved in 'Ser-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of serine ('Ser-72' in the arylsulfatase AtsA) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemica

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994973, Length=387, Percent_Identity=35.4005167958656, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI87081923, Length=368, Percent_Identity=33.695652173913, Blast_Score=229, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44812; Mature: 44812

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLNTLRQQQIPVMTEYRAQIPFHILAKPIGPACNLACRYCYYPQGETPVEKMNESTLE
CCCCHHHHHHCCCCHHHHHHCCCHHEEECCCCCHHHHHEEEEECCCCCCCHHHHCHHHHH
VFICRYIAAQPASAREINFVWQGGEPLLAGIGFYKKVIALQQRYAPDDVTISNSLQTNAT
HHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHH
LLNDAWCRLFRDNNFTIGISLEGSEDLQNYHRPGKRGESSYPAVLRGITLLQHYRVDFNV
HHHHHHHHHEECCCEEEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
LIVVHDDMARHAAAIYDHVVSLGARYLQFQPLMNEGNALQQRYQLSADNWGRFMIDIWRQ
EEEEECCHHHHHHHHHHHHHHHHHHHHEECCHHCCCHHHHHHHHCCCCCCCHHHHHHHHH
WRKRGDMGRVFVINIEQAWAQYFTHISATCVHSARCGTNLVMEPDGKLYACDHLINSQHY
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHCCHHH
LGQLSNNMLAPAVDTATRLPFGIKKSQRRECQRCSVKIVCQGGCPAHINSAGYNRLCSGY
HHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHH
YSFFTEILAPLRAWPRNLNGLKAWRADVMGRFSG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNLNTLRQQQIPVMTEYRAQIPFHILAKPIGPACNLACRYCYYPQGETPVEKMNESTLE
CCCCHHHHHHCCCCHHHHHHCCCHHEEECCCCCHHHHHEEEEECCCCCCCHHHHCHHHHH
VFICRYIAAQPASAREINFVWQGGEPLLAGIGFYKKVIALQQRYAPDDVTISNSLQTNAT
HHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHH
LLNDAWCRLFRDNNFTIGISLEGSEDLQNYHRPGKRGESSYPAVLRGITLLQHYRVDFNV
HHHHHHHHHEECCCEEEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
LIVVHDDMARHAAAIYDHVVSLGARYLQFQPLMNEGNALQQRYQLSADNWGRFMIDIWRQ
EEEEECCHHHHHHHHHHHHHHHHHHHHEECCHHCCCHHHHHHHHCCCCCCCHHHHHHHHH
WRKRGDMGRVFVINIEQAWAQYFTHISATCVHSARCGTNLVMEPDGKLYACDHLINSQHY
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEHHHHCCHHH
LGQLSNNMLAPAVDTATRLPFGIKKSQRRECQRCSVKIVCQGGCPAHINSAGYNRLCSGY
HHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHH
YSFFTEILAPLRAWPRNLNGLKAWRADVMGRFSG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180918 [H]