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The map label for this gene is pykA [H]

Identifier: 194735271

GI number: 194735271

Start: 1961040

End: 1962482

Strand: Direct

Name: pykA [H]

Synonym: SeSA_A2042

Alternate gene names: 194735271

Gene position: 1961040-1962482 (Clockwise)

Preceding gene: 194736565

Following gene: 194738340

Centisome position: 41.64

GC content: 55.51

Gene sequence:

>1443_bases
ATGTCCAGAAGGCTTCGCAGAACCAAAATCGTTACCACGTTAGGCCCGGCAACTGACCGCGATAACAACCTTGAGAAGGT
TATCGCCGCGGGCGCAAACGTAGTACGTATGAACTTCTCTCACGGCTCGCCGGAAGATCATAAAATGCGTGCTGATAAAG
TCCGTGAGATTGCCGCCAAACTGGGGCGTCATGTGGCTATTTTAGGCGACCTGCAAGGGCCAAAAATTCGCGTTTCCACT
TTTAAAGAAGGCAAAGTGTTCCTCAACATCGGGGACAAATTTCTGTTAGACGCTAACCTGGGTAAAGGCGAAGGCGATAA
AGAAAAAGTCGGCATTGATTACAAAGGGTTGCCGGCAGACGTCGTTCCTGGCGATATCCTGCTGCTTGACGATGGTCGAG
TGCAGTTGAAAGTGCTTGAAGTCCAGGGCATGAAAGTCTTTACCGAGGTCACCGTCGGCGGCCCGCTGTCTAACAATAAA
GGCATTAACAAGCTCGGCGGCGGGCTTTCTGCTGAAGCGCTGACCGAAAAAGACAAAGCCGATATTCAAACCGCTGCGCT
GATAGGCGTTGACTATCTGGCTGTCTCCTTCCCGCGCTGCGGCGAAGATCTGAACTATGCACGCCGCCTGGCGCGCGACG
CCGGCTGCGACGCGAAAATTGTGGCTAAGGTCGAACGCGCCGAAGCGGTATGCGATCAAAACGCCATGGACGATATCATT
CTGGCCTCTGACGTTGTGATGGTCGCACGTGGCGACCTGGGCGTTGAAATCGGCGATCCGGAGCTGGTTGGTATCCAGAA
GGCGCTGATTCGCCGTGCGCGTCAGCTAAACCGCGCAGTCATCACCGCAACGCAGATGATGGAGTCGATGATCACCAACC
CGATGCCGACCCGTGCGGAAGTGATGGACGTGGCGAACGCCGTCCTGGATGGCACGGATGCGGTTATGCTGTCTGCCGAA
ACCGCAGCCGGTCAGTATCCTTCTGAAACCGTTGCCGCAATGGCGCGCGTCTGCCTGGGCGCAGAAAAAATCCCCAGCAT
CAATGTGTCTAAACACCGTCTCGACGTGCAGTTCGACAACGTTGAAGAAGCCATTGCCATGTCTGCGATGTATGCGGCAA
ACCATCTGAAAGGCGTTACCGCGATCATCACCATGACGGAATCCGGTCGTACCGCGCTAATGACTTCCCGTATCAGCTCC
GGCCTGCCGATTTTCGCCATGTCGCGCCATGAACGCACGCTGAACCTGACCGCGCTCTACCGCGGAGTAACGCCGGTGCA
TTTTGATAGCGCGGCTGATGGCGTTGTCGCGGCGCATGAAGCTGTTAATCTGCTGCGCGATAAAGGGTATCTGGTTTCCG
GCGACCTGGTGATCGTGACTCAGGGCGATGTCATGAGCACCGTCGGCTCAACCAATACCACGCGTATTTTGACCGTTGAG
TAA

Upstream 100 bases:

>100_bases
TTTCGTCATCCGGGGACGTTCATTTTATACCGTTAAGGTTTCAGAACGACCGGATCAATGTTCACGCAACACCAAGTTGT
TTTAGTCAACGGAGTATTAC

Downstream 100 bases:

>100_bases
AACTCCCCCACGCCTGTTCATCAGGTAGTACAGGGTTTGTCAGCATAAAGCCTCTCTTACGAGAGGCTTTATTTATTTGA
TGGGATAAAGATCTTTACGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCDQNAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSAADGVVAAHEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE

Sequences:

>Translated_480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCDQNAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSAADGVVAAHEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE
>Mature_479_residues
SRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTF
KEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKG
INKLGGGLSAEALTEKDKADIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCDQNAMDDIIL
ASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSG
LPIFAMSRHERTLNLTALYRGVTPVHFDSAADGVVAAHEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=36.8525896414343, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=36.8525896414343, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=36.969696969697, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=35.2226720647773, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=35.2226720647773, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=35.5172413793103, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=35.5172413793103, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.4728033472803, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.4728033472803, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.0232558139535, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=98.5416666666667, Blast_Score=955, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=487, Percent_Identity=36.9609856262834, Blast_Score=280, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=36.40081799591, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=38.0566801619433, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=38.0566801619433, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=38.0566801619433, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=38.0566801619433, Blast_Score=245, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=36.1224489795918, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=499, Percent_Identity=34.8697394789579, Blast_Score=249, Evalue=7e-67,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.4493927125506, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.4493927125506, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24648966, Length=418, Percent_Identity=35.8851674641148, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=32.1792260692464, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=35.6617647058824, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51388; Mature: 51257

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAK
CCCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHH
DIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCDQNAMDDII
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEE
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAE
ECCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEE
RGVTPVHFDSAADGVVAAHEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE
CCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAK
CCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHH
DIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCDQNAMDDII
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEE
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAE
ECCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEE
RGVTPVHFDSAADGVVAAHEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE
CCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]