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The map label for this gene is hemK

Identifier: 194735200

GI number: 194735200

Start: 1848523

End: 1849356

Strand: Reverse

Name: hemK

Synonym: SeSA_A1912

Alternate gene names: 194735200

Gene position: 1849356-1848523 (Counterclockwise)

Preceding gene: 194736546

Following gene: 194734672

Centisome position: 39.27

GC content: 58.51

Gene sequence:

>834_bases
ATGGATTTTCAGCACTGGCTGCATGAGGCGGTAAACCAGCTCCGGGACAGCGACAGCCCCCGGCGCGACGCCGAGATCCT
GCTTGAGTACGTTACGGGCAAGGGGCGGACGTATATCATGGCCTTTGGCGAAACGCCGCTTACTGACGTCCAGCAACAAC
AGCTCGCGGATCTGCTGCAGCGGCGTAAACAGGGCGAACCCATTGCGTACCTGACGGGCTTACGCGAATTCTGGTCGCTG
CCGCTCTTCGTTTCTCCCGCCACGCTAATCCCGCGCCCGGATACCGAATGTCTGGTTGAACAGGCGTTGGCGCGATTGCC
GGTCAAAACCTGTCGTATTCTGGATTTAGGCACCGGAACTGGCGCGATTGCGCTGGCGCTGGCCTGCGAGCGACCGGATT
GTGAGGTGACTGCGGTCGATCGTATGCCTGATGCGGTTGCGCTGGCGATACGCAACGCGGAACATTTGGCTATCCGGAAT
GTGCGTATACTGCAAAGTTGTTGGTTCAGCGCGCTATCGGGGCAGCAGTTCGACATGATTGTCAGCAATCCGCCCTACAT
TGACGCGCAGGACCCGCACCTTAGCGAAGGCGATGTACGCTTTGAACCGCGCTCGGCGTTGGTGGCGGACGAAAACGGTA
TGGCGGATCTTACGCATATTATCGATAATGCCCGCCAGATGTTAACGCCCGGCGGCTTCCTGCTGCTGGAACACGGCTGG
CAGCAGGGCGAAGCGGTAAGAGCGGTTTTCAGGCGGTCTGGTTACTCGGATGTAGAAACGTGTCGTGATTATGGTGGTAA
TGAACGCGTGACCTGCGGACGTTTTACGCCATGA

Upstream 100 bases:

>100_bases
GCCTTGATGAAACGATGGAAGGTAAGTTGGATATGCTGATTGAGCCGATTGTTCAGGAACATCAGGCTGACCTGTTAGCC
GCGTTGTCCGAGCAGGAATA

Downstream 100 bases:

>100_bases
CAACTGCAATGTTGCTCACGCTTCATCTTATCTGTGTTGCGCTCTCCGTCAGTTTGTTTGTCGCTCGTTACTGGTGGCGC
TATTGCGGCCATGCGTTGGC

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKGRTYIMAFGETPLTDVQQQQLADLLQRRKQGEPIAYLTGLREFWSL
PLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGTGAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRN
VRILQSCWFSALSGQQFDMIVSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
QQGEAVRAVFRRSGYSDVETCRDYGGNERVTCGRFTP

Sequences:

>Translated_277_residues
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKGRTYIMAFGETPLTDVQQQQLADLLQRRKQGEPIAYLTGLREFWSL
PLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGTGAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRN
VRILQSCWFSALSGQQFDMIVSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
QQGEAVRAVFRRSGYSDVETCRDYGGNERVTCGRFTP
>Mature_277_residues
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKGRTYIMAFGETPLTDVQQQQLADLLQRRKQGEPIAYLTGLREFWSL
PLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGTGAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRN
VRILQSCWFSALSGQQFDMIVSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
QQGEAVRAVFRRSGYSDVETCRDYGGNERVTCGRFTP

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252'

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=251, Percent_Identity=31.4741035856574, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=77.0909090909091, Blast_Score=442, Evalue=1e-126,
Organism=Escherichia coli, GI87082085, Length=196, Percent_Identity=36.734693877551, Blast_Score=108, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324266, Length=180, Percent_Identity=31.1111111111111, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582226, Length=225, Percent_Identity=28.8888888888889, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_SALTY (P40816)

Other databases:

- EMBL:   AE006468
- EMBL:   J04243
- PIR:   C32890
- RefSeq:   NP_460731.1
- ProteinModelPortal:   P40816
- SMR:   P40816
- PRIDE:   P40816
- GeneID:   1253294
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1775
- HOGENOM:   HBG732041
- OMA:   YVSEYTR
- ProtClustDB:   PRK09328
- BioCyc:   STYP99287:STM1775-MONOMER
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 30967; Mature: 30967

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 140-140 BINDING 168-168 BINDING 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKGRTYIMAFGETPLTDVQQQQLADLLQ
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
RRKQGEPIAYLTGLREFWSLPLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGT
HHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHEEEEECCCCC
GAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRNVRILQSCWFSALSGQQFDMI
CCEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
VSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
QQGEAVRAVFRRSGYSDVETCRDYGGNERVTCGRFTP
CCHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCCC
>Mature Secondary Structure
MDFQHWLHEAVNQLRDSDSPRRDAEILLEYVTGKGRTYIMAFGETPLTDVQQQQLADLLQ
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
RRKQGEPIAYLTGLREFWSLPLFVSPATLIPRPDTECLVEQALARLPVKTCRILDLGTGT
HHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHEEEEECCCCC
GAIALALACERPDCEVTAVDRMPDAVALAIRNAEHLAIRNVRILQSCWFSALSGQQFDMI
CCEEEEEECCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
VSNPPYIDAQDPHLSEGDVRFEPRSALVADENGMADLTHIIDNARQMLTPGGFLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
QQGEAVRAVFRRSGYSDVETCRDYGGNERVTCGRFTP
CCHHHHHHHHHHCCCCHHHHHHHCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564