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The map label for this gene is gdhA [H]

Identifier: 194734885

GI number: 194734885

Start: 1871589

End: 1872863

Strand: Direct

Name: gdhA [H]

Synonym: SeSA_A1936

Alternate gene names: 194734885

Gene position: 1871589-1872863 (Clockwise)

Preceding gene: 194737941

Following gene: 194737640

Centisome position: 39.74

GC content: 56.94

Gene sequence:

>1275_bases
ATGGATAAGTTATCTTACGCTTCAGATAGCAGCACATCTGCCTGGAATACCTACCTGCAACAAATCGAGCGTGTGGCCCC
TTATCTGGGCGAGTTATCCCCCTGGGTGGATACTCTGCGCCACCCAAAACGCGCTCTGATCGTCGATATTCCGGTACAAA
TGGATGATGGCACTATTCGTCATTTCGAAGGATATCGCGTGCAGCACAACCTCTCCCGAGGTCCGGGTAAAGGCGGTGTT
CGCTACCATCCTGACGTTGATCTCAATGAAGTGATGGCCCTGTCAGCATGGATGACCATCAAATGTGCCGCGCTGAATCT
GCCGTACGGCGGCGCCAAAGGCGGCATCCGCGTCGATCCGTTCTCGCTGTCGGAAGGTGAACTGGAGCGTTTGACCCGCC
GCTATACCAGCGAGATCGGCATTATCATCGGGCCGCAGAAAGATATTCCTGCGCCGGATGTCGGCACCAACGGTAAAGTG
ATGGCCTGGATGATGGATACGTATTCCATGAATCATGGCACGACGGTCACTGGCGTCGTCACCGGTAAGCCTATCCATCT
TGGCGGTTCGCTGGGCCGTGAAAAAGCGACGGGGCGCGGCGTTTTCGTCAGCGGGCTGGAAGCCGCGCGTCGGGCGAATA
TCGCCGTTGAAGGCGCTCGCGTTGCGGTTCAGGGTTTTGGTAACGTCGGCAGCGAAGCCGCGCGTCTGTTTGCAGGCGCT
GGCGCCCGTGTCGTGGCGATTCAGGATCATACCGCAACGCTGTTTAACGCCACCGGTATTGACATGAAGGCGCTCACGGC
ATGGCAGACAGAACACAAACAGATAGCCGGTTTCCCGGGCGCGGAAACTATCGCCAGCGATGCGTTCTGGCGTCTGGAGA
TGGATATTCTGATCCCGGCGGCGCTTGAAGGCCAGATAACTCGTCAGCGCGCGGAAGCCCTGACGTGTAAGCTGGTGCTG
GAAGGGGCTAACGGCCCGACCTATCCGGATGCTGATGATGTTCTGGCCAGCCGTGGTATCCTTGTGGTGCCTGACGTGGT
CTGTAACGCAGGCGGCGTAACCGTCAGCTACTTCGAATGGGTACAAGATATGGCCAGCTTCTTCTGGAGCGAAGAAGAGA
TCAACGCGCGCATGGACAAAATCATGACCGACGCTATCGTCCATGTCTGGGAGAAAGCGACTGAGAAATCCTGTTCTCTG
CGCACCGCAGCCTATATTGTCGCCTGTGAGCGCATTCTGCTGGCCCGTAAAGATCGCGGTATCTATCCAGGTTAA

Upstream 100 bases:

>100_bases
TAATTGTTTAAAAAAGTGTTAAATTTATCGCTACATGGTGTGATCTACTATGTACCACGGTCAATTAAAGAACATATTAC
TTTTATCACTAAGGTTTATC

Downstream 100 bases:

>100_bases
GGCGACAAATCATTGCGCTAAAATCCACGTCCCGGCGTGGATTTTTTGTCTGTATACCCCGTTTTCCACGCGCGCGCCGA
TAAATTCTGTCTCAATAAAA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MDKLSYASDSSTSAWNTYLQQIERVAPYLGELSPWVDTLRHPKRALIVDIPVQMDDGTIRHFEGYRVQHNLSRGPGKGGV
RYHPDVDLNEVMALSAWMTIKCAALNLPYGGAKGGIRVDPFSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKV
MAWMMDTYSMNHGTTVTGVVTGKPIHLGGSLGREKATGRGVFVSGLEAARRANIAVEGARVAVQGFGNVGSEAARLFAGA
GARVVAIQDHTATLFNATGIDMKALTAWQTEHKQIAGFPGAETIASDAFWRLEMDILIPAALEGQITRQRAEALTCKLVL
EGANGPTYPDADDVLASRGILVVPDVVCNAGGVTVSYFEWVQDMASFFWSEEEINARMDKIMTDAIVHVWEKATEKSCSL
RTAAYIVACERILLARKDRGIYPG

Sequences:

>Translated_424_residues
MDKLSYASDSSTSAWNTYLQQIERVAPYLGELSPWVDTLRHPKRALIVDIPVQMDDGTIRHFEGYRVQHNLSRGPGKGGV
RYHPDVDLNEVMALSAWMTIKCAALNLPYGGAKGGIRVDPFSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKV
MAWMMDTYSMNHGTTVTGVVTGKPIHLGGSLGREKATGRGVFVSGLEAARRANIAVEGARVAVQGFGNVGSEAARLFAGA
GARVVAIQDHTATLFNATGIDMKALTAWQTEHKQIAGFPGAETIASDAFWRLEMDILIPAALEGQITRQRAEALTCKLVL
EGANGPTYPDADDVLASRGILVVPDVVCNAGGVTVSYFEWVQDMASFFWSEEEINARMDKIMTDAIVHVWEKATEKSCSL
RTAAYIVACERILLARKDRGIYPG
>Mature_424_residues
MDKLSYASDSSTSAWNTYLQQIERVAPYLGELSPWVDTLRHPKRALIVDIPVQMDDGTIRHFEGYRVQHNLSRGPGKGGV
RYHPDVDLNEVMALSAWMTIKCAALNLPYGGAKGGIRVDPFSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKV
MAWMMDTYSMNHGTTVTGVVTGKPIHLGGSLGREKATGRGVFVSGLEAARRANIAVEGARVAVQGFGNVGSEAARLFAGA
GARVVAIQDHTATLFNATGIDMKALTAWQTEHKQIAGFPGAETIASDAFWRLEMDILIPAALEGQITRQRAEALTCKLVL
EGANGPTYPDADDVLASRGILVVPDVVCNAGGVTVSYFEWVQDMASFFWSEEEINARMDKIMTDAIVHVWEKATEKSCSL
RTAAYIVACERILLARKDRGIYPG

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=333, Percent_Identity=44.1441441441441, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI4885281, Length=333, Percent_Identity=42.6426426426426, Blast_Score=270, Evalue=1e-72,
Organism=Escherichia coli, GI1788059, Length=362, Percent_Identity=33.1491712707182, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17544676, Length=381, Percent_Identity=38.8451443569554, Blast_Score=262, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6319256, Length=416, Percent_Identity=29.3269230769231, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=375, Percent_Identity=29.0666666666667, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI45549226, Length=331, Percent_Identity=44.1087613293051, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI45553475, Length=331, Percent_Identity=44.1087613293051, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24649283, Length=330, Percent_Identity=44.5454545454545, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649460, Length=281, Percent_Identity=44.4839857651246, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46027; Mature: 46027

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00074 GLFV_DEHYDROGENASE ; PS00137 SUBTILASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLSYASDSSTSAWNTYLQQIERVAPYLGELSPWVDTLRHPKRALIVDIPVQMDDGTIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEEEECCCCEE
HFEGYRVQHNLSRGPGKGGVRYHPDVDLNEVMALSAWMTIKCAALNLPYGGAKGGIRVDP
ECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCCEEECC
FSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKVMAWMMDTYSMNHGTTVTGVV
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEE
TGKPIHLGGSLGREKATGRGVFVSGLEAARRANIAVEGARVAVQGFGNVGSEAARLFAGA
ECCEEEECCCCCCCCCCCCCEEEECHHHHHHCCEEEECCEEEEEECCCCCHHHHHHHHCC
GARVVAIQDHTATLFNATGIDMKALTAWQTEHKQIAGFPGAETIASDAFWRLEMDILIPA
CEEEEEEECCCEEEEECCCCCHHHHHHHHHCCHHHCCCCCHHHHHCCCEEEEEEEEEECC
ALEGQITRQRAEALTCKLVLEGANGPTYPDADDVLASRGILVVPDVVCNAGGVTVSYFEW
HHCCHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHCCEEEECHHHCCCCCCCHHHHHH
VQDMASFFWSEEEINARMDKIMTDAIVHVWEKATEKSCSLRTAAYIVACERILLARKDRG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
IYPG
CCCC
>Mature Secondary Structure
MDKLSYASDSSTSAWNTYLQQIERVAPYLGELSPWVDTLRHPKRALIVDIPVQMDDGTIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEEEECCCCEE
HFEGYRVQHNLSRGPGKGGVRYHPDVDLNEVMALSAWMTIKCAALNLPYGGAKGGIRVDP
ECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCCEEECC
FSLSEGELERLTRRYTSEIGIIIGPQKDIPAPDVGTNGKVMAWMMDTYSMNHGTTVTGVV
CCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEE
TGKPIHLGGSLGREKATGRGVFVSGLEAARRANIAVEGARVAVQGFGNVGSEAARLFAGA
ECCEEEECCCCCCCCCCCCCEEEECHHHHHHCCEEEECCEEEEEECCCCCHHHHHHHHCC
GARVVAIQDHTATLFNATGIDMKALTAWQTEHKQIAGFPGAETIASDAFWRLEMDILIPA
CEEEEEEECCCEEEEECCCCCHHHHHHHHHCCHHHCCCCCHHHHHCCCEEEEEEEEEECC
ALEGQITRQRAEALTCKLVLEGANGPTYPDADDVLASRGILVVPDVVCNAGGVTVSYFEW
HHCCHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHCCEEEECHHHCCCCCCCHHHHHH
VQDMASFFWSEEEINARMDKIMTDAIVHVWEKATEKSCSLRTAAYIVACERILLARKDRG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
IYPG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]