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The map label for this gene is mrdA [H]

Identifier: 194734378

GI number: 194734378

Start: 1982399

End: 1984270

Strand: Direct

Name: mrdA [H]

Synonym: SeSA_A2065

Alternate gene names: 194734378

Gene position: 1982399-1984270 (Clockwise)

Preceding gene: 194737429

Following gene: 194736100

Centisome position: 42.1

GC content: 52.14

Gene sequence:

>1872_bases
ATGACTTTTAAAGACTTTGATGCGGAAGAGAAACTCTTCCTGAGACGTGTGATTGTGGCGTTTGGTGTAGTGGTTGTCTG
CTTTGGTATCCTGATCTTCAACCTCTATAACTTGCAAATCCGCCAGCACCACTACTACACCACCCGTTCGAATGAGAACG
ATATCAAGATGCTACCCGTTGCGCCCACGCGCGGCATCATTTACGATCGCAACGGCATTCCGCTGGTGCGTAACGTAACC
TGGTATGATATCGCGGTTACGCCCTATAAAATTGCCGATATGGATGCGCTGCTAAAACAGCTCACCCCTATTGTTGATCT
CTCCCCCGATGATATTGCCGACTTTCGCCATGCGCTAAAATCCAGTAGCCGCTATCGTCCGGTGGTGCTCAAAAACGCGC
TAACGGATGTTGAAATCGCACGCTTTGCGGTCAATCAGTTTCATTTCAACGGCGTCACGATCAATAGCTATCAGGATCGG
CAGTATCCTTACGGCGCGGAGCTGGCGCATGTTCTCGGTTACGTGTCAAAGATAAACGATAACGACCTTAAAGCGCTGGA
TAAAAAAGGGCTGGCAGAGAATTACGCGGCGGACCATAACATCGGTAAACAGGGGATTGAGCGTTACTACGAAAACGATC
TTCATGGCAAAACCGGTTATCAGGAGGTCGAGGTGGATAATCACGGGCGTATTGTCCGCCTGCTGAAAGACGTTCCTCCC
ATCGCGGGTAAGAATATTCACCTGACGCTGGATCTCCATTTACAAGAGTACATTGAAAGTTTGCTGGCCGGGCAGCGTGC
TGCCGTACTGGTTGAAGATCCGCACGATGGTTCAGTGCTGGCGATGGTATCTATGCCCAGCTACGACCCGAACCCCTTTG
TAAAAGGCATTAGCTATCAGGATTACGGCAAACTGCTGCACGATAAAAATCTGCCGCTGATCAACCGCGTGACGCAAGGG
CTTTATCCCCCGGCGTCAACGGTTAAACCCTATATGGCGATGTCCGCGCTGTTATGCGGGATTATTACGCCGCAAACCAC
CTTCTTTGGCGCGCCAACCTGGACGCTTCCCGGCACGCAGCGGCATTACCGGGACTGGAAAAAAACCGGACACGGTATGC
TGGATGTCACTAAAGCGATTGAGGAATCTGCGGATACCTTTTTCTATCAGGTTGCTTATATGATGGGTATTGACCGTATC
GACACGATGTTGTCTCAGTTCGGCTATGGCAAACCGACGGGGATCGACCTTAATGAAGAGTATGACGGGCTGCTTCCCAG
CCGCGCATGGAAACAGCGGGTCCATAAAAAAGCCTGGTATCAGGGCGATACCATCTCTGTCGGCATCGGCCAGGGCTACT
GGATTGCCACCCCCATCCAGATGGTAAAAGCGATGGTGGCGCTCATCAACAACGGTAAGGTGATTGCCCCTCACCTGTTA
CTCAATGAAGAGAGCGGCAAAACGGTGGTTCCGTATCGCCCTTCAGGAACACCGGCACAGATAGCCGATCCGGCGTCGCC
ATACTGGGGACTGGTGCGTCAGGCGATGTACGGCATGGCGAATGCACCCAACGGAACGGGCTATAAGTTCTTTCACACCG
CGCCCTACGGCATTGCGGCCAAAAGCGGCACGTCGCAGGTGTTTAGCCTGAAAGAAAATCAGACCTACAATGCGAAAATG
ATCCCCATTCGCCTGCGCGATCATGTGTTTTATACCGCTTTTGCACCGTATAAAAACCCTAAGGTCGCTATTGCCTTGAT
TCTGGAAAACGGCGGAAGCGACGGCGTTACCGCCGCGCCCATCATGCGAAAAATCCTTGACCACCTGTTTGATCCACAGG
CTGATACCACACAGTCGGGTCAGGCGCCATAG

Upstream 100 bases:

>100_bases
CAAGGAATGACACATTTCTATAGAGAGATGGAACATCTTTTACCTGTGCTGGCGCTGACCGCCCCGTATCATTAGGCGGC
GGATTGTTCAGGTACAGGTA

Downstream 100 bases:

>100_bases
TCAAATAAGCAAAGCGCCATCAGACAAGGATACGACCGGAATATCCGCATACATCAGAAATAGTTACGCAGATATTCCGT
CAAACACAGCATAGCCATCG

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPVAPTRGIIYDRNGIPLVRNVT
WYDIAVTPYKIADMDALLKQLTPIVDLSPDDIADFRHALKSSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDR
QYPYGAELAHVLGYVSKINDNDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQDYGKLLHDKNLPLINRVTQG
LYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQRHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRI
DTMLSQFGYGKPTGIDLNEEYDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAAKSGTSQVFSLKENQTYNAKM
IPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAPIMRKILDHLFDPQADTTQSGQAP

Sequences:

>Translated_623_residues
MTFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPVAPTRGIIYDRNGIPLVRNVT
WYDIAVTPYKIADMDALLKQLTPIVDLSPDDIADFRHALKSSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDR
QYPYGAELAHVLGYVSKINDNDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQDYGKLLHDKNLPLINRVTQG
LYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQRHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRI
DTMLSQFGYGKPTGIDLNEEYDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAAKSGTSQVFSLKENQTYNAKM
IPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAPIMRKILDHLFDPQADTTQSGQAP
>Mature_622_residues
TFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPVAPTRGIIYDRNGIPLVRNVTW
YDIAVTPYKIADMDALLKQLTPIVDLSPDDIADFRHALKSSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDRQ
YPYGAELAHVLGYVSKINDNDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPPI
AGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQDYGKLLHDKNLPLINRVTQGL
YPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQRHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRID
TMLSQFGYGKPTGIDLNEEYDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLLL
NEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAAKSGTSQVFSLKENQTYNAKMI
PIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAPIMRKILDHLFDPQADTTQSGQAP

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=623, Percent_Identity=58.5874799357945, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=635, Percent_Identity=22.3622047244094, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69731; Mature: 69599

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEEC
APTRGIIYDRNGIPLVRNVTWYDIAVTPYKIADMDALLKQLTPIVDLSPDDIADFRHALK
CCCCCEEECCCCCCEEECCEEEEEEECCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
SSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDRQYPYGAELAHVLGYVSKIND
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCCCCCHHHHHHHHHHHHHCCC
NDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
CHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQ
CCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHCCCCHH
DYGKLLHDKNLPLINRVTQGLYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQ
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCH
RHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRIDTMLSQFGYGKPTGIDLNEE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCEEEEEEE
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAA
EECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEE
KSGTSQVFSLKENQTYNAKMIPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAP
CCCCCCEEEECCCCCCCEEEEEEEEECCEEEEEECCCCCCCEEEEEEEECCCCCCCCHHH
IMRKILDHLFDPQADTTQSGQAP
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEEC
APTRGIIYDRNGIPLVRNVTWYDIAVTPYKIADMDALLKQLTPIVDLSPDDIADFRHALK
CCCCCEEECCCCCCEEECCEEEEEEECCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
SSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDRQYPYGAELAHVLGYVSKIND
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCCCCCHHHHHHHHHHHHHCCC
NDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
CHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQ
CCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHCCCCHH
DYGKLLHDKNLPLINRVTQGLYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQ
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCH
RHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRIDTMLSQFGYGKPTGIDLNEE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCEEEEEEE
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAA
EECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEE
KSGTSQVFSLKENQTYNAKMIPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAP
CCCCCCEEEECCCCCCCEEEEEEEEECCEEEEEECCCCCCCEEEEEEEECCCCCCCCHHH
IMRKILDHLFDPQADTTQSGQAP
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]