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The map label for this gene is kdsB

Identifier: 194334665

GI number: 194334665

Start: 2040143

End: 2040892

Strand: Reverse

Name: kdsB

Synonym: Paes_1866

Alternate gene names: 194334665

Gene position: 2040892-2040143 (Counterclockwise)

Preceding gene: 194334666

Following gene: 194334663

Centisome position: 81.22

GC content: 52.93

Gene sequence:

>750_bases
ATGCCTCCAATCAATATCGTCATTCTGATTCCCGCAAGGCTCAATTCCAGCAGACTGCCCAATAAAATGCTTGCCGATAT
CGAGGGAGCTCCGCTGATTGTCAGAACGTGGCAGCAGGCGCAGCAGTCAAGCCTGACCCGGCAGGTGGTTATCGCTACAG
ACAGTGAGGAGATTGCCGGTGTGATGAAGGCGTGTGGCGCCGATGTCGTTATGACCTCCCCTGACGCGCGTTGCGGTACC
GAGCGGATTGCTGAAGCGGCCGAAAATATTGATGCAGACGTGTTTGTCAATCTGCAGGGCGACGAACCGCTGATCGATCC
GGGGAATATCGACCTGGTTATCAAACCGTTTCTCCAGGAGAACCCTCCGGACTGCTCGACGTTGGTCTATCGGCTTCTTG
CGGAGGATTATGCCGCATTGCATGATCCCAACATCGTCAAAGTGGTCATGGACAGTCAGTCCAATGCGCTTTATTTTTCC
AGGAGCCCTCTGCCGTATCAGCGAGAAACCTATGCGGCAACCCGTTGCTTTCGTCATATCGGTATTTATGCCTTTCGCGC
AGGTGTGCTGAGGATGTTTTCAGAACACGGGCCTTCAATGCTTGAGGAAGCGGAGTCTCTCGAACAGCTTCGTCTTGTCG
AAAATGGTTATAGCATTCGCTGTGTAGAGACCGATGTCGATATGCCGGGCGTCAATACTCATGAGGATCTTGAACTTGTT
CGTCGCCTTTATCGCTCCCGTCGGTCATGA

Upstream 100 bases:

>100_bases
CATGATTCTTGATACCCCATGATTTGAGCACATGACAAGCAGGCTCAGTTGTGCAGATAACCTGCTTCATATACCGGTCT
AACACGTAACAGGTTCCGCT

Downstream 100 bases:

>100_bases
TCGGGATTTCGCCGTCGTAAAATACTGCCGATGCCCGCTTTTTCCTGGCCATGTCACGGCTGGTCGTGATATAGCCGCTC
TCTGTTTTGATCAAGGCAAA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPPINIVILIPARLNSSRLPNKMLADIEGAPLIVRTWQQAQQSSLTRQVVIATDSEEIAGVMKACGADVVMTSPDARCGT
ERIAEAAENIDADVFVNLQGDEPLIDPGNIDLVIKPFLQENPPDCSTLVYRLLAEDYAALHDPNIVKVVMDSQSNALYFS
RSPLPYQRETYAATRCFRHIGIYAFRAGVLRMFSEHGPSMLEEAESLEQLRLVENGYSIRCVETDVDMPGVNTHEDLELV
RRLYRSRRS

Sequences:

>Translated_249_residues
MPPINIVILIPARLNSSRLPNKMLADIEGAPLIVRTWQQAQQSSLTRQVVIATDSEEIAGVMKACGADVVMTSPDARCGT
ERIAEAAENIDADVFVNLQGDEPLIDPGNIDLVIKPFLQENPPDCSTLVYRLLAEDYAALHDPNIVKVVMDSQSNALYFS
RSPLPYQRETYAATRCFRHIGIYAFRAGVLRMFSEHGPSMLEEAESLEQLRLVENGYSIRCVETDVDMPGVNTHEDLELV
RRLYRSRRS
>Mature_248_residues
PPINIVILIPARLNSSRLPNKMLADIEGAPLIVRTWQQAQQSSLTRQVVIATDSEEIAGVMKACGADVVMTSPDARCGTE
RIAEAAENIDADVFVNLQGDEPLIDPGNIDLVIKPFLQENPPDCSTLVYRLLAEDYAALHDPNIVKVVMDSQSNALYFSR
SPLPYQRETYAATRCFRHIGIYAFRAGVLRMFSEHGPSMLEEAESLEQLRLVENGYSIRCVETDVDMPGVNTHEDLELVR
RLYRSRRS

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=248, Percent_Identity=41.1290322580645, Blast_Score=168, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_PROA2 (B4S4I2)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016525.1
- ProteinModelPortal:   B4S4I2
- SMR:   B4S4I2
- GeneID:   6460149
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1866
- HOGENOM:   HBG637773
- OMA:   FSRAPLP
- ProtClustDB:   PRK05450
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27833; Mature: 27702

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPINIVILIPARLNSSRLPNKMLADIEGAPLIVRTWQQAQQSSLTRQVVIATDSEEIAG
CCCEEEEEEEECCCCCCCCCHHHHHCCCCCCEEHHHHHHHHHHCCCEEEEEEECHHHHHH
VMKACGADVVMTSPDARCGTERIAEAAENIDADVFVNLQGDEPLIDPGNIDLVIKPFLQE
HHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECHHCC
NPPDCSTLVYRLLAEDYAALHDPNIVKVVMDSQSNALYFSRSPLPYQRETYAATRCFRHI
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHH
GIYAFRAGVLRMFSEHGPSMLEEAESLEQLRLVENGYSIRCVETDVDMPGVNTHEDLELV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
RRLYRSRRS
HHHHHHHCC
>Mature Secondary Structure 
PPINIVILIPARLNSSRLPNKMLADIEGAPLIVRTWQQAQQSSLTRQVVIATDSEEIAG
CCEEEEEEEECCCCCCCCCHHHHHCCCCCCEEHHHHHHHHHHCCCEEEEEEECHHHHHH
VMKACGADVVMTSPDARCGTERIAEAAENIDADVFVNLQGDEPLIDPGNIDLVIKPFLQE
HHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECHHCC
NPPDCSTLVYRLLAEDYAALHDPNIVKVVMDSQSNALYFSRSPLPYQRETYAATRCFRHI
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHH
GIYAFRAGVLRMFSEHGPSMLEEAESLEQLRLVENGYSIRCVETDVDMPGVNTHEDLELV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
RRLYRSRRS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA