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The map label for this gene is pheA [H]

Identifier: 194333338

GI number: 194333338

Start: 535074

End: 535913

Strand: Reverse

Name: pheA [H]

Synonym: Paes_0495

Alternate gene names: 194333338

Gene position: 535913-535074 (Counterclockwise)

Preceding gene: 194333342

Following gene: 194333337

Centisome position: 21.33

GC content: 50.36

Gene sequence:

>840_bases
ATGACAAACCGCTTGGTTGCATATCAGGGCGAGCCCGGCGCATACAGCGAAATCGCGGCCCTTCGCTTCGGCCAACCAGA
ACCATTTGAATCGTTTGACGATGCATTCAATGCCGTCGAAAACAAACAGGTCGCTTGTGCGGTCATTCCTATCGAAAATT
CACTTGGGGGAAGCATACATCACAACTACGACCTCCTGATAGAGCACCCGGTACATATCGTCGCCGAAACATTTGTCAAG
GTCCAGCACTGCCTTCTCGGATTACCGGGATCATCAACGGCATCACCGGGCAATGTCCTCTCTCATCCACAGGCACTTGC
CCAATGCAGGAATTTCTTCAACCGCCACCCTCACCTCAAAGCTGAAGTCGCTTACGATACTGCGGGCAGCGCAAAAATCA
TCGCTTCGGAAGGCAAGAGCAGCAATCTCGCCATTGCTTCGAAACGTGCTGCCGAGCTCTACGGCCTCTCCATTCTCCAG
GAAAACCTGGCTGATGAAGAGTGGAACATCACCCGTTTTTTCTGCATCGCCCATGAACATCATAGAGAAAACCTCCCGTT
CCTGCAAACAGAGGAATCGGAAAGACAAAAAACCTCGATTGTCTTTACCCTTCCCAATGAACCGGGCTCTCTCTTCAAAG
CCCTGGCGACCTTTGCCCTGCGCAGCATCGATCTGACAAAAATTGAATCGCGCCCGTTCCGCAAAAAAGCGTTTGAATAC
CTTTTCTATGTCGATTGCATAGGCCATAGCGACGATCAGAACGTCCGGAACGCACTGGGACATCTCAAAGAATTTGCAAC
GATGGTAAAAGTATTAGGCAGTTATGGCATTGTCAAGTAA

Upstream 100 bases:

>100_bases
TGACCTCGTAAGATTGGCAAGGGCTCATCATTCTGACCCGCCAGAAGTAAATAAAATCAAATCAATCTGCTTTACCGCAA
ATTAATCACAAGAAAAAATA

Downstream 100 bases:

>100_bases
TGAAAATCAGTTCCAGCTCTCCCTGGACGAAACAACGCAAGGAGTTGCAGAACCCTCACTGCCAGACACACCGCGTACTG
AAAAACCCGAGCTTTTCCTT

Product: Prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTNRLVAYQGEPGAYSEIAALRFGQPEPFESFDDAFNAVENKQVACAVIPIENSLGGSIHHNYDLLIEHPVHIVAETFVK
VQHCLLGLPGSSTASPGNVLSHPQALAQCRNFFNRHPHLKAEVAYDTAGSAKIIASEGKSSNLAIASKRAAELYGLSILQ
ENLADEEWNITRFFCIAHEHHRENLPFLQTEESERQKTSIVFTLPNEPGSLFKALATFALRSIDLTKIESRPFRKKAFEY
LFYVDCIGHSDDQNVRNALGHLKEFATMVKVLGSYGIVK

Sequences:

>Translated_279_residues
MTNRLVAYQGEPGAYSEIAALRFGQPEPFESFDDAFNAVENKQVACAVIPIENSLGGSIHHNYDLLIEHPVHIVAETFVK
VQHCLLGLPGSSTASPGNVLSHPQALAQCRNFFNRHPHLKAEVAYDTAGSAKIIASEGKSSNLAIASKRAAELYGLSILQ
ENLADEEWNITRFFCIAHEHHRENLPFLQTEESERQKTSIVFTLPNEPGSLFKALATFALRSIDLTKIESRPFRKKAFEY
LFYVDCIGHSDDQNVRNALGHLKEFATMVKVLGSYGIVK
>Mature_278_residues
TNRLVAYQGEPGAYSEIAALRFGQPEPFESFDDAFNAVENKQVACAVIPIENSLGGSIHHNYDLLIEHPVHIVAETFVKV
QHCLLGLPGSSTASPGNVLSHPQALAQCRNFFNRHPHLKAEVAYDTAGSAKIIASEGKSSNLAIASKRAAELYGLSILQE
NLADEEWNITRFFCIAHEHHRENLPFLQTEESERQKTSIVFTLPNEPGSLFKALATFALRSIDLTKIESRPFRKKAFEYL
FYVDCIGHSDDQNVRNALGHLKEFATMVKVLGSYGIVK

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=278, Percent_Identity=31.6546762589928, Blast_Score=147, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6324013, Length=291, Percent_Identity=27.4914089347079, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 30982; Mature: 30851

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRLVAYQGEPGAYSEIAALRFGQPEPFESFDDAFNAVENKQVACAVIPIENSLGGSIH
CCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEE
HNYDLLIEHPVHIVAETFVKVQHCLLGLPGSSTASPGNVLSHPQALAQCRNFFNRHPHLK
CCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCE
AEVAYDTAGSAKIIASEGKSSNLAIASKRAAELYGLSILQENLADEEWNITRFFCIAHEH
EEEEECCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHH
HRENLPFLQTEESERQKTSIVFTLPNEPGSLFKALATFALRSIDLTKIESRPFRKKAFEY
HCCCCCCEECCCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHH
LFYVDCIGHSDDQNVRNALGHLKEFATMVKVLGSYGIVK
HHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNRLVAYQGEPGAYSEIAALRFGQPEPFESFDDAFNAVENKQVACAVIPIENSLGGSIH
CCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEE
HNYDLLIEHPVHIVAETFVKVQHCLLGLPGSSTASPGNVLSHPQALAQCRNFFNRHPHLK
CCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCE
AEVAYDTAGSAKIIASEGKSSNLAIASKRAAELYGLSILQENLADEEWNITRFFCIAHEH
EEEEECCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHH
HRENLPFLQTEESERQKTSIVFTLPNEPGSLFKALATFALRSIDLTKIESRPFRKKAFEY
HCCCCCCEECCCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHH
LFYVDCIGHSDDQNVRNALGHLKEFATMVKVLGSYGIVK
HHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1919506; 10368439 [H]