Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 194333300

GI number: 194333300

Start: 490548

End: 491183

Strand: Reverse

Name: nth [H]

Synonym: Paes_0457

Alternate gene names: 194333300

Gene position: 491183-490548 (Counterclockwise)

Preceding gene: 194333301

Following gene: 194333296

Centisome position: 19.55

GC content: 50.47

Gene sequence:

>636_bases
ATGAATGTTGCAGAAAAAATCGCCTTCATCAATGAAGCCCTGGGAAACAAATATCCCTCACCAAAAAGCGAGCTGCAGTA
CCAGAGCCCTTATCAGTTGCTCATCGCGACCATGCTTGCAGCGCAGTCTACCGATAAAAAGGTCAATATGATTACAGAGA
CCCTCTTCAAGGTCTGTCCCGATGCCGAGAGCATGAGCCGGACTGATCCGGAAGAGATCCGCTCCATGGTACGATCGATC
AATTACAATAATACCAAAGCAAAAAATATTCTTGCTGCCAGTTGCCTGCTGATGGAAAACTTCGGCGGCCAGGTTCCTGA
TTCACGAGAAGAACTTGAAACGTTGCCCGGAGTCGGACGCAAAACGGCAAATGTGGTTCTGAGCAACGCATTCGGAAAAC
CGGTCATGCCCGTCGACACCCATGTCCACCGGGTTTCTAACCGAATCGGACTGGTAGCCACCGATAACCCGAGAGATACC
GAAGACGGATTGATCGCAATTATTCCTGAAAACAGGGTGATAGACTTCCACCACTACCTGCTGCTCCATGGCCGCTACAC
CTGCAAGGCCCGCAAACCGCTCTGCAGCGAGTGTCCTCTTGTCCCTGCATGTGATTATCCGGCAAAGAATTACTGA

Upstream 100 bases:

>100_bases
TGCGTTTACAAGGACGATCCGTCTCTGGGAAAGCTTTCTGCCATAATACTCCCCTCCCGTCAACTGGTCCAGACAGTAAA
CATCGATTAAACCGACCATT

Downstream 100 bases:

>100_bases
GAAGCTTCTGATGGAAGCCATCCATGACGTGCTGAATTGATCAAATGCTGCAGCGTAGACAGAAGACCGGTTCAACACGT
CAGCAATTTGTCAGATCAGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNVAEKIAFINEALGNKYPSPKSELQYQSPYQLLIATMLAAQSTDKKVNMITETLFKVCPDAESMSRTDPEEIRSMVRSI
NYNNTKAKNILAASCLLMENFGGQVPDSREELETLPGVGRKTANVVLSNAFGKPVMPVDTHVHRVSNRIGLVATDNPRDT
EDGLIAIIPENRVIDFHHYLLLHGRYTCKARKPLCSECPLVPACDYPAKNY

Sequences:

>Translated_211_residues
MNVAEKIAFINEALGNKYPSPKSELQYQSPYQLLIATMLAAQSTDKKVNMITETLFKVCPDAESMSRTDPEEIRSMVRSI
NYNNTKAKNILAASCLLMENFGGQVPDSREELETLPGVGRKTANVVLSNAFGKPVMPVDTHVHRVSNRIGLVATDNPRDT
EDGLIAIIPENRVIDFHHYLLLHGRYTCKARKPLCSECPLVPACDYPAKNY
>Mature_211_residues
MNVAEKIAFINEALGNKYPSPKSELQYQSPYQLLIATMLAAQSTDKKVNMITETLFKVCPDAESMSRTDPEEIRSMVRSI
NYNNTKAKNILAASCLLMENFGGQVPDSREELETLPGVGRKTANVVLSNAFGKPVMPVDTHVHRVSNRIGLVATDNPRDT
EDGLIAIIPENRVIDFHHYLLLHGRYTCKARKPLCSECPLVPACDYPAKNY

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=171, Percent_Identity=30.9941520467836, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI115298654, Length=221, Percent_Identity=25.7918552036199, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298652, Length=221, Percent_Identity=25.7918552036199, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI190358497, Length=221, Percent_Identity=25.7918552036199, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298648, Length=221, Percent_Identity=25.7918552036199, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI6912520, Length=221, Percent_Identity=25.7918552036199, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298650, Length=221, Percent_Identity=25.7918552036199, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=42.5837320574163, Blast_Score=183, Evalue=7e-48,
Organism=Escherichia coli, GI1789331, Length=188, Percent_Identity=27.1276595744681, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=183, Percent_Identity=32.2404371584699, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=173, Percent_Identity=27.1676300578035, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550361, Length=171, Percent_Identity=29.2397660818713, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23496; Mature: 23496

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAEKIAFINEALGNKYPSPKSELQYQSPYQLLIATMLAAQSTDKKVNMITETLFKVCP
CCHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
DAESMSRTDPEEIRSMVRSINYNNTKAKNILAASCLLMENFGGQVPDSREELETLPGVGR
CHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
KTANVVLSNAFGKPVMPVDTHVHRVSNRIGLVATDNPRDTEDGLIAIIPENRVIDFHHYL
HHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCEEHHHHEE
LLHGRYTCKARKPLCSECPLVPACDYPAKNY
EHCCCEEECCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNVAEKIAFINEALGNKYPSPKSELQYQSPYQLLIATMLAAQSTDKKVNMITETLFKVCP
CCHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
DAESMSRTDPEEIRSMVRSINYNNTKAKNILAASCLLMENFGGQVPDSREELETLPGVGR
CHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
KTANVVLSNAFGKPVMPVDTHVHRVSNRIGLVATDNPRDTEDGLIAIIPENRVIDFHHYL
HHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCEEHHHHEE
LLHGRYTCKARKPLCSECPLVPACDYPAKNY
EHCCCEEECCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]