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The map label for this gene is mrcA [H]

Identifier: 194333160

GI number: 194333160

Start: 340982

End: 343234

Strand: Reverse

Name: mrcA [H]

Synonym: Paes_0316

Alternate gene names: 194333160

Gene position: 343234-340982 (Counterclockwise)

Preceding gene: 194333161

Following gene: 194333159

Centisome position: 13.66

GC content: 52.24

Gene sequence:

>2253_bases
GTGACGACGAAAAAGAAACATCAACCCGGCAAAAAAAACAGACTCCCTCTTCTTCCCGTAATCGGCGCGTTCATTGCAGC
GATCACGATCACGGGGATCATCTATATTTTCGGGATGACACGCTCGCTTCCCGGCATTGAAGAGCTTGAGAACCCCAATC
CCGAACTGGCATCGCTGGTCTACTCGAGGGACGGAAAGCTGCTGCACAAGTATTTCATGAAAAACCGCACCTACGTGCCG
CTGAAATCCATCTCGGAGTACCTCCCGAAAGCGCTTATTGCAACAGAAGACCTTGCGTTTTATGACCACTGGGGATTCGA
TGTAAGAAGATTTATCCTGGCCATGGGCGAAAACATGCTCAAGGGGCGTGACCGGTGGCATGGCGCAAGCACCATAACCC
AGCAGCTGGCCAAAAACCTGTTCCTCACCCAGGAACGGACTGTCGACCGAAAAATCAAGGAGTTCTTCACCTCGATCGAG
CTGGAAAAAACCTATACGAAAGAAGAAATTCTCGCACTCTACCTCAACACCGTCTACTTCGGATCCGGTGCCTATGGTGT
GGAAGCGGCCGCATGGACCTATTTCAACAAGCCCGCCAGCACATTGAACCTGCAGGAAAGCGCAACCCTGATAGCGGTCC
TCAAAAGCCCGAGAGCCTATGATCCGTCACGGAATCCCGAAAGTTCCATCAACCGCCGGAACCTCATCCTCAGCCTGATG
GCAAAAGAAGGGGTCATCACGGAAACAGAAGCCGAAAAGGCCAGAAAAAGCAAGCTCATCCTTGACTATACGCCAGTGAC
AAACCACGGCAAAGCGCCGTACTTCACCGAATATATCCGTCAGACGCTCAAACCGATCTCAAAACAGCACGACATCAATG
TCTACAAAGACGGTCTCGTGATCTACACCTCGCTCGACAGCCGCATGCAACGCTATGCGCAGGAGGCCGTCAAGGAACAT
CTTGCATGGGTACAGGAACGGTTCGACAAATCATGGCGCTGGCCAGCAAAACTCAAGGATCAGATCATCAGAGAAAGTCC
CCGTTACAGGGAACTGACTGAAAAAGGACGGTCAAAAGATCAGGCGATGAAAGAACTCAAAGCCGACGCACCATGGCTGA
AAAAACTGCTCTACGATAAAACACGTGTTCAGGCTGCCTTTGTAATAATCGAGCCTTCGACAGGAGAAATCCTCGCCTGG
GTAGGAGGCAACGACTTCACACCTGAAGAGTACCAGTACCAGTTCGATCATGTCTGGCAGGCTCGACGTCAGCCGGGCTC
GACCTTCAAACCGTTTGTCTATGCCGCCGCAATCGATAAAGGTATTCCCGCAAATTTCGAAATCCTCGACCAGCCGCTTG
TCCTCAAGACAGGCAACGATGTCTGGATACCCCAGAACGCTGACCAGAAATCAGGCGGACTGACGCCGTTGCGAAAAGCA
CTGAGCAATTCAATCAATCAGGTCACCGTCCGACTGCTTCACGAATTTCTCAGCCCGTCCGAGGTAATCCGCTACGCAAA
GAAAATGGGCATCACCTCGAAGCTTGAACCAAACATGTCGATCGCCCTCGGCACATCGGAGGTCTCTCCCCTCGAACTGG
CATCGGCATTCGGTACGTTTGCCAACAACGGCGTCTGGGTGGAGCCGACCAGCATTACCAGTATCGAGGATAAATTCCAC
CACAAGGTAATCGAATACGATCCGGTCAAACGCACCGCTCTTGATTCTACGACAAACTATGTCGTTGTCTCGATGCTGCA
GGACGTCATCAAAAGAGGAACAGGCATCGCCGTTCCATCGAGATACGGCATCAAGCTGGAAGCCGGAGGCAAAACCGGCA
CGACACAGAACCTGAAAGACGCATGGTTTGTCGGATTCACCCCCCAGATTGTCGGAGCCGTCTGGACAGGATTTGACGAC
GAACGCATCGCGTTCACCTCCATGAGTTATGGTCAGGGAGCTCGCGCGGCGCTTCCGATCTGGGCTAAATTCATGCAGAA
ATGCATTGCCGACACCACTATCGGCATTGAAAACCGATACTTCCACATGCCGGAAAACGTCATTGCCGTTCCGGTTTCAC
GATCAACGAACCGGCCAGCCCAGCTCTACACCGACGATGTCTACGTTGAGTACTTTACACCGAAAGGATTTAAAAAGTAC
CAGTCCGAGCTGGCTACTCCTTCAGAAGAGCAATTGATGCCGGCAAGCGACAACACCTATGAATCACCGGGATGGCAGGA
AGACCGGTACTGA

Upstream 100 bases:

>100_bases
ATGATGAAGTCACCAAAATCTCATGACTGACAGACCGAAGGAACACCCGGGCACAGACGAAAACGATTGCATTACTTTGT
AACTCATACTACGACCTATT

Downstream 100 bases:

>100_bases
TTTTCTATTCAACCACTTAATGTTCACGTTTATGCAGTCTGTTCTTGTACTCGATTTCGGCTCTCAATATACCCAGCTCA
TTGCCAGGAGAATCCGCGAA

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MTTKKKHQPGKKNRLPLLPVIGAFIAAITITGIIYIFGMTRSLPGIEELENPNPELASLVYSRDGKLLHKYFMKNRTYVP
LKSISEYLPKALIATEDLAFYDHWGFDVRRFILAMGENMLKGRDRWHGASTITQQLAKNLFLTQERTVDRKIKEFFTSIE
LEKTYTKEEILALYLNTVYFGSGAYGVEAAAWTYFNKPASTLNLQESATLIAVLKSPRAYDPSRNPESSINRRNLILSLM
AKEGVITETEAEKARKSKLILDYTPVTNHGKAPYFTEYIRQTLKPISKQHDINVYKDGLVIYTSLDSRMQRYAQEAVKEH
LAWVQERFDKSWRWPAKLKDQIIRESPRYRELTEKGRSKDQAMKELKADAPWLKKLLYDKTRVQAAFVIIEPSTGEILAW
VGGNDFTPEEYQYQFDHVWQARRQPGSTFKPFVYAAAIDKGIPANFEILDQPLVLKTGNDVWIPQNADQKSGGLTPLRKA
LSNSINQVTVRLLHEFLSPSEVIRYAKKMGITSKLEPNMSIALGTSEVSPLELASAFGTFANNGVWVEPTSITSIEDKFH
HKVIEYDPVKRTALDSTTNYVVVSMLQDVIKRGTGIAVPSRYGIKLEAGGKTGTTQNLKDAWFVGFTPQIVGAVWTGFDD
ERIAFTSMSYGQGARAALPIWAKFMQKCIADTTIGIENRYFHMPENVIAVPVSRSTNRPAQLYTDDVYVEYFTPKGFKKY
QSELATPSEEQLMPASDNTYESPGWQEDRY

Sequences:

>Translated_750_residues
MTTKKKHQPGKKNRLPLLPVIGAFIAAITITGIIYIFGMTRSLPGIEELENPNPELASLVYSRDGKLLHKYFMKNRTYVP
LKSISEYLPKALIATEDLAFYDHWGFDVRRFILAMGENMLKGRDRWHGASTITQQLAKNLFLTQERTVDRKIKEFFTSIE
LEKTYTKEEILALYLNTVYFGSGAYGVEAAAWTYFNKPASTLNLQESATLIAVLKSPRAYDPSRNPESSINRRNLILSLM
AKEGVITETEAEKARKSKLILDYTPVTNHGKAPYFTEYIRQTLKPISKQHDINVYKDGLVIYTSLDSRMQRYAQEAVKEH
LAWVQERFDKSWRWPAKLKDQIIRESPRYRELTEKGRSKDQAMKELKADAPWLKKLLYDKTRVQAAFVIIEPSTGEILAW
VGGNDFTPEEYQYQFDHVWQARRQPGSTFKPFVYAAAIDKGIPANFEILDQPLVLKTGNDVWIPQNADQKSGGLTPLRKA
LSNSINQVTVRLLHEFLSPSEVIRYAKKMGITSKLEPNMSIALGTSEVSPLELASAFGTFANNGVWVEPTSITSIEDKFH
HKVIEYDPVKRTALDSTTNYVVVSMLQDVIKRGTGIAVPSRYGIKLEAGGKTGTTQNLKDAWFVGFTPQIVGAVWTGFDD
ERIAFTSMSYGQGARAALPIWAKFMQKCIADTTIGIENRYFHMPENVIAVPVSRSTNRPAQLYTDDVYVEYFTPKGFKKY
QSELATPSEEQLMPASDNTYESPGWQEDRY
>Mature_749_residues
TTKKKHQPGKKNRLPLLPVIGAFIAAITITGIIYIFGMTRSLPGIEELENPNPELASLVYSRDGKLLHKYFMKNRTYVPL
KSISEYLPKALIATEDLAFYDHWGFDVRRFILAMGENMLKGRDRWHGASTITQQLAKNLFLTQERTVDRKIKEFFTSIEL
EKTYTKEEILALYLNTVYFGSGAYGVEAAAWTYFNKPASTLNLQESATLIAVLKSPRAYDPSRNPESSINRRNLILSLMA
KEGVITETEAEKARKSKLILDYTPVTNHGKAPYFTEYIRQTLKPISKQHDINVYKDGLVIYTSLDSRMQRYAQEAVKEHL
AWVQERFDKSWRWPAKLKDQIIRESPRYRELTEKGRSKDQAMKELKADAPWLKKLLYDKTRVQAAFVIIEPSTGEILAWV
GGNDFTPEEYQYQFDHVWQARRQPGSTFKPFVYAAAIDKGIPANFEILDQPLVLKTGNDVWIPQNADQKSGGLTPLRKAL
SNSINQVTVRLLHEFLSPSEVIRYAKKMGITSKLEPNMSIALGTSEVSPLELASAFGTFANNGVWVEPTSITSIEDKFHH
KVIEYDPVKRTALDSTTNYVVVSMLQDVIKRGTGIAVPSRYGIKLEAGGKTGTTQNLKDAWFVGFTPQIVGAVWTGFDDE
RIAFTSMSYGQGARAALPIWAKFMQKCIADTTIGIENRYFHMPENVIAVPVSRSTNRPAQLYTDDVYVEYFTPKGFKKYQ
SELATPSEEQLMPASDNTYESPGWQEDRY

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=610, Percent_Identity=31.3114754098361, Blast_Score=268, Evalue=1e-72,
Organism=Escherichia coli, GI1786343, Length=630, Percent_Identity=29.5238095238095, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1788867, Length=602, Percent_Identity=25.5813953488372, Blast_Score=149, Evalue=8e-37,
Organism=Escherichia coli, GI1789601, Length=236, Percent_Identity=36.0169491525424, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 85154; Mature: 85023

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKKKHQPGKKNRLPLLPVIGAFIAAITITGIIYIFGMTRSLPGIEELENPNPELASLV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHH
YSRDGKLLHKYFMKNRTYVPLKSISEYLPKALIATEDLAFYDHWGFDVRRFILAMGENML
HHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCHHH
KGRDRWHGASTITQQLAKNLFLTQERTVDRKIKEFFTSIELEKTYTKEEILALYLNTVYF
HCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GSGAYGVEAAAWTYFNKPASTLNLQESATLIAVLKSPRAYDPSRNPESSINRRNLILSLM
CCCCCCCCHHHEEEECCCCCCCCCHHCCEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHH
AKEGVITETEAEKARKSKLILDYTPVTNHGKAPYFTEYIRQTLKPISKQHDINVYKDGLV
HCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEE
IYTSLDSRMQRYAQEAVKEHLAWVQERFDKSWRWPAKLKDQIIRESPRYRELTEKGRSKD
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHH
QAMKELKADAPWLKKLLYDKTRVQAAFVIIEPSTGEILAWVGGNDFTPEEYQYQFDHVWQ
HHHHHHHCCCHHHHHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHH
ARRQPGSTFKPFVYAAAIDKGIPANFEILDQPLVLKTGNDVWIPQNADQKSGGLTPLRKA
HHCCCCCCCCCCEEEEHHCCCCCCCCEEECCCEEEECCCEEECCCCCCCCCCCCHHHHHH
LSNSINQVTVRLLHEFLSPSEVIRYAKKMGITSKLEPNMSIALGTSEVSPLELASAFGTF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
ANNGVWVEPTSITSIEDKFHHKVIEYDPVKRTALDSTTNYVVVSMLQDVIKRGTGIAVPS
CCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEECCC
RYGIKLEAGGKTGTTQNLKDAWFVGFTPQIVGAVWTGFDDERIAFTSMSYGQGARAALPI
CCCEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
WAKFMQKCIADTTIGIENRYFHMPENVIAVPVSRSTNRPAQLYTDDVYVEYFTPKGFKKY
HHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCCCCCCCEEEECCEEEEEECCCCHHHH
QSELATPSEEQLMPASDNTYESPGWQEDRY
HHHHCCCCHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTKKKHQPGKKNRLPLLPVIGAFIAAITITGIIYIFGMTRSLPGIEELENPNPELASLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHH
YSRDGKLLHKYFMKNRTYVPLKSISEYLPKALIATEDLAFYDHWGFDVRRFILAMGENML
HHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCHHH
KGRDRWHGASTITQQLAKNLFLTQERTVDRKIKEFFTSIELEKTYTKEEILALYLNTVYF
HCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GSGAYGVEAAAWTYFNKPASTLNLQESATLIAVLKSPRAYDPSRNPESSINRRNLILSLM
CCCCCCCCHHHEEEECCCCCCCCCHHCCEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHH
AKEGVITETEAEKARKSKLILDYTPVTNHGKAPYFTEYIRQTLKPISKQHDINVYKDGLV
HCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCEE
IYTSLDSRMQRYAQEAVKEHLAWVQERFDKSWRWPAKLKDQIIRESPRYRELTEKGRSKD
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCHH
QAMKELKADAPWLKKLLYDKTRVQAAFVIIEPSTGEILAWVGGNDFTPEEYQYQFDHVWQ
HHHHHHHCCCHHHHHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHH
ARRQPGSTFKPFVYAAAIDKGIPANFEILDQPLVLKTGNDVWIPQNADQKSGGLTPLRKA
HHCCCCCCCCCCEEEEHHCCCCCCCCEEECCCEEEECCCEEECCCCCCCCCCCCHHHHHH
LSNSINQVTVRLLHEFLSPSEVIRYAKKMGITSKLEPNMSIALGTSEVSPLELASAFGTF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
ANNGVWVEPTSITSIEDKFHHKVIEYDPVKRTALDSTTNYVVVSMLQDVIKRGTGIAVPS
CCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEECCC
RYGIKLEAGGKTGTTQNLKDAWFVGFTPQIVGAVWTGFDDERIAFTSMSYGQGARAALPI
CCCEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
WAKFMQKCIADTTIGIENRYFHMPENVIAVPVSRSTNRPAQLYTDDVYVEYFTPKGFKKY
HHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCCCCCCCEEEECCEEEEEECCCCHHHH
QSELATPSEEQLMPASDNTYESPGWQEDRY
HHHHCCCCHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]