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The map label for this gene is mazG [H]

Identifier: 194333059

GI number: 194333059

Start: 227903

End: 228724

Strand: Reverse

Name: mazG [H]

Synonym: Paes_0215

Alternate gene names: 194333059

Gene position: 228724-227903 (Counterclockwise)

Preceding gene: 194333064

Following gene: 194333058

Centisome position: 9.1

GC content: 51.46

Gene sequence:

>822_bases
ATGGAACCATCACAGAACCAAAATATCGCACGCCTCCGCGACGAGGTGCTTAACAACAAAGGCACCTCACTGCAGGAAAA
ATTTGACCGGGTGCTGAATCTGGTGAAAGTGCTGCGTTCCGAATGTCCGTGGGACCGGAAACAGACCCCGCAATCGCTTG
CCCACCTGCTGCTTGAAGAGAGTTATGAACTGGTGCACGCTATCGACGAAGGGGATCAGCAAGAACTCAAAAAAGAGCTG
GGCGACCTCTTTCTGCATATCTCTTTTCAGGTACTCATGGCCGAAGAGGCCAAAACATTCACCTATGCTGAGGTCTTCGA
CGCGCTCTGCGACAAATTGATCAACCGCCATCCGCATGTCTTCGGCGACACCACCGCAGAAAACGAGCAGGACGTACTCA
AAAACTGGGAGTCATTGAAACTCCTTGAAAAAAACCGCCGGAGCCTGCTCGACGGTGTTCCGAAAGCCATGTCCGAACTG
CTCCGGGCCTACCGGGTTCAGAAAAAAGTTGCCGGAGTCGGTTTCGACTGGCAAAGCGATGAAGGGGTTCTCGACAAACT
GACCGAGGAAATAGCAGAACTCAAAAACGCAAAAACCTCTGCAGAACAGGAAGAGGAGTTCGGCGACCTGCTCTTCACCA
TCGTCAACTATAGCCGCTTTATCAGCACCAATCCCGAAGACGCTCTGCGCAAAGCGACAAACAAGTTCATGAGCCAGTTT
ATGCATGTCGAAAAGATGGTGCAGGCATCTGAAAAAAGCTGGAGGGAGTTCTCCCCCGAAGAACTTGATGCACTGTGGAA
CAGCGCAAAAAAAGCGCTGTAA

Upstream 100 bases:

>100_bases
TGAGTTCAGGATATGCTGCTGAACAAAAGTACAGAAAATCAGACAGAAATATTAATTTGCCCCCACTTGCAATAACCATT
ATCAGTAGCGGCAACAACTC

Downstream 100 bases:

>100_bases
AGCCTATATTGGGGTAAGAGCTGCAAAACTCCTTCTATAGCCGGCTGTCGATCATCCATTCCCGACAAAACCAACAGGCC
CATGCAATATCTCAATGCTC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEPSQNQNIARLRDEVLNNKGTSLQEKFDRVLNLVKVLRSECPWDRKQTPQSLAHLLLEESYELVHAIDEGDQQELKKEL
GDLFLHISFQVLMAEEAKTFTYAEVFDALCDKLINRHPHVFGDTTAENEQDVLKNWESLKLLEKNRRSLLDGVPKAMSEL
LRAYRVQKKVAGVGFDWQSDEGVLDKLTEEIAELKNAKTSAEQEEEFGDLLFTIVNYSRFISTNPEDALRKATNKFMSQF
MHVEKMVQASEKSWREFSPEELDALWNSAKKAL

Sequences:

>Translated_273_residues
MEPSQNQNIARLRDEVLNNKGTSLQEKFDRVLNLVKVLRSECPWDRKQTPQSLAHLLLEESYELVHAIDEGDQQELKKEL
GDLFLHISFQVLMAEEAKTFTYAEVFDALCDKLINRHPHVFGDTTAENEQDVLKNWESLKLLEKNRRSLLDGVPKAMSEL
LRAYRVQKKVAGVGFDWQSDEGVLDKLTEEIAELKNAKTSAEQEEEFGDLLFTIVNYSRFISTNPEDALRKATNKFMSQF
MHVEKMVQASEKSWREFSPEELDALWNSAKKAL
>Mature_273_residues
MEPSQNQNIARLRDEVLNNKGTSLQEKFDRVLNLVKVLRSECPWDRKQTPQSLAHLLLEESYELVHAIDEGDQQELKKEL
GDLFLHISFQVLMAEEAKTFTYAEVFDALCDKLINRHPHVFGDTTAENEQDVLKNWESLKLLEKNRRSLLDGVPKAMSEL
LRAYRVQKKVAGVGFDWQSDEGVLDKLTEEIAELKNAKTSAEQEEEFGDLLFTIVNYSRFISTNPEDALRKATNKFMSQF
MHVEKMVQASEKSWREFSPEELDALWNSAKKAL

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=256, Percent_Identity=39.84375, Blast_Score=186, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 31522; Mature: 31522

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]