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The map label for this gene is asnB [H]

Identifier: 194332961

GI number: 194332961

Start: 117217

End: 118995

Strand: Reverse

Name: asnB [H]

Synonym: Paes_0114

Alternate gene names: 194332961

Gene position: 118995-117217 (Counterclockwise)

Preceding gene: 194332962

Following gene: 194332960

Centisome position: 4.74

GC content: 54.08

Gene sequence:

>1779_bases
ATGTGTGGAATTGCAGGAGAACTCCGATTCGATAGTGCAGCAGCCTCTTCAACGACTGTCGACGCAATGCTCGGCATTCT
TGAGCCAAGAGGACCTGACGCACAGGGAGTCTTCGCCCACAACAGGATCTGTTTCGGGCACCGGCGTCTGAAAATCATCG
ATCTTTCAGACTGCGCATCCCAACCGATGGTCGATAGTGCTCTCGGTCTGGTTCTCGTCTTCAACGGCGCCGTCTACAAC
TACCGGGAACTTTGTACAGAACTCGTCGGGAAAGGCTACAGCTTCTTCTCTCAATCAGATACAGAGATCATTATCAAGGC
GTACCACGCCTGGGGAGAAGATTGTGTCGGCCACCTGCAGGGAATGTTTGCGTTTGCCATCTGGGAACGCGATACAGGCA
GAACGTTTTTTGCCCGTGACCGCCTTGGAATCAAACCCCTCTACTTCACAGAGGATAACGCCTCGATGCGCTTCGCATCA
TCCCTTCCGGCGCTCCTGAAATCAGGCGCTGTCGATACGGCTATCGACCCGGCTGCGCTCGACTGCTATATGAGTTTTCA
CTCGGTCGTTCCAGCCCCCTGGACCATCTTTCGGGGCATACGCAAGCTGCCGCCGGCAACAACCATGATGGTCGAGCCTG
ACGGCAGGAAACAGCAGCGAACTTACTGGCAACCAGACTACTCACGATCGGATGAAGAACGCAGGCGAAGTGCCGGAGAG
TGGATTGATGCCGTCCATGATGCGCTGAAGCTTGCTGTACAACGCCGTTTGGTAGCAGACGTACCGGTAGGAGTTCTGCT
TTCCGGAGGTCTTGACTCATCTCTGGTTGTCGGACTGCTCAGTGAACTCGTGCAACGCCACCTCAGCACCTTCTCCGTCG
GCTTCGAGGATGTCGCCGAAGAGGAAGGCAACGAGTTCCGGTATTCCGACATCATCGCTGAACGCTACAGCACCGATCAC
CATAAAATCTTCGTTGACCACAGAGAACTGCAGACCCATCTCTCAGACTGCGTCAGAGCGATGTCGGAACCGATGGTAAG
CCACGATGTAATAGGATTCTACCTCCTCTCGCGCGAAGTCAGCCAACACGTCAGGGTAGTGCAGAGCGGCCAGGGCGCCG
ACGAGGTCTTCGCCGGGTACCACTGGTATCCGCCGATGATGAACACTCCGCCGACAGAGGCATACAGCACCTACTCAGGC
GTTTTCTTCGACCGCGACTTCAACGAATACCGTCAGGCTGTCAACAGTTCGTTTATCACCGAAGACCACGCTGCTGCATT
TGTCAGAAACCACTTTGCCATGCCGGGTGCCGAAGAACCCATCGACAAGGCGCTCCGGCTCGACAGTACCATCATGCTCG
TTGACGATCCGGTCAAAAGGGTAGACAACATGACCATGGCATGGGGGCTTGAGGCCAGAGTACCGTTTCTTGATCATGAA
CTGGTTGAACTGGCAGCAAAAATTCCCGCAGAATACAAAGTCAAGGACGGCGGCAAATACGTACTCAAGGAGGTAGGCCG
CAGGATCATTCCTCACGAGGTGATCGACCGCCCGAAAGGCTACTTTCCCGTCCCTGCGCTCAAGTATCTCGAAGGGGAAT
ACCTTGACATGGCAAAAAATATTCTCAACCAGGATGCAGCAAAACAACGCAAAATCTTCAACAGAGAGTATGTCGATATG
CTGCTCAATGCGCCTCAAGATCATATCACTCCGCTCAGAGGATCGAAACTCTGGCAGATAACACTCCTTGAATACTGGCT
GCAGGAGCAGGGACTATGA

Upstream 100 bases:

>100_bases
CGGGTAGATCCCGGCAGAGGAACGCAGGCTCCGCCAGACAATTCCTCCCCCGGAAAGACCCAGGCGTCATCTGAACGATA
CAGAAGAATACCAACCCGTT

Downstream 100 bases:

>100_bases
CAAAAAAACTCCGAAGCGAAGAGCATCTTGTACCGATGCAGTCCCCGAGCATGAAAAGCTGGGGGATGCCTGCCGATCTG
CCAAAACATAACGTTGTCAT

Product: asparagine synthase family amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MCGIAGELRFDSAAASSTTVDAMLGILEPRGPDAQGVFAHNRICFGHRRLKIIDLSDCASQPMVDSALGLVLVFNGAVYN
YRELCTELVGKGYSFFSQSDTEIIIKAYHAWGEDCVGHLQGMFAFAIWERDTGRTFFARDRLGIKPLYFTEDNASMRFAS
SLPALLKSGAVDTAIDPAALDCYMSFHSVVPAPWTIFRGIRKLPPATTMMVEPDGRKQQRTYWQPDYSRSDEERRRSAGE
WIDAVHDALKLAVQRRLVADVPVGVLLSGGLDSSLVVGLLSELVQRHLSTFSVGFEDVAEEEGNEFRYSDIIAERYSTDH
HKIFVDHRELQTHLSDCVRAMSEPMVSHDVIGFYLLSREVSQHVRVVQSGQGADEVFAGYHWYPPMMNTPPTEAYSTYSG
VFFDRDFNEYRQAVNSSFITEDHAAAFVRNHFAMPGAEEPIDKALRLDSTIMLVDDPVKRVDNMTMAWGLEARVPFLDHE
LVELAAKIPAEYKVKDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGEYLDMAKNILNQDAAKQRKIFNREYVDM
LLNAPQDHITPLRGSKLWQITLLEYWLQEQGL

Sequences:

>Translated_592_residues
MCGIAGELRFDSAAASSTTVDAMLGILEPRGPDAQGVFAHNRICFGHRRLKIIDLSDCASQPMVDSALGLVLVFNGAVYN
YRELCTELVGKGYSFFSQSDTEIIIKAYHAWGEDCVGHLQGMFAFAIWERDTGRTFFARDRLGIKPLYFTEDNASMRFAS
SLPALLKSGAVDTAIDPAALDCYMSFHSVVPAPWTIFRGIRKLPPATTMMVEPDGRKQQRTYWQPDYSRSDEERRRSAGE
WIDAVHDALKLAVQRRLVADVPVGVLLSGGLDSSLVVGLLSELVQRHLSTFSVGFEDVAEEEGNEFRYSDIIAERYSTDH
HKIFVDHRELQTHLSDCVRAMSEPMVSHDVIGFYLLSREVSQHVRVVQSGQGADEVFAGYHWYPPMMNTPPTEAYSTYSG
VFFDRDFNEYRQAVNSSFITEDHAAAFVRNHFAMPGAEEPIDKALRLDSTIMLVDDPVKRVDNMTMAWGLEARVPFLDHE
LVELAAKIPAEYKVKDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGEYLDMAKNILNQDAAKQRKIFNREYVDM
LLNAPQDHITPLRGSKLWQITLLEYWLQEQGL
>Mature_592_residues
MCGIAGELRFDSAAASSTTVDAMLGILEPRGPDAQGVFAHNRICFGHRRLKIIDLSDCASQPMVDSALGLVLVFNGAVYN
YRELCTELVGKGYSFFSQSDTEIIIKAYHAWGEDCVGHLQGMFAFAIWERDTGRTFFARDRLGIKPLYFTEDNASMRFAS
SLPALLKSGAVDTAIDPAALDCYMSFHSVVPAPWTIFRGIRKLPPATTMMVEPDGRKQQRTYWQPDYSRSDEERRRSAGE
WIDAVHDALKLAVQRRLVADVPVGVLLSGGLDSSLVVGLLSELVQRHLSTFSVGFEDVAEEEGNEFRYSDIIAERYSTDH
HKIFVDHRELQTHLSDCVRAMSEPMVSHDVIGFYLLSREVSQHVRVVQSGQGADEVFAGYHWYPPMMNTPPTEAYSTYSG
VFFDRDFNEYRQAVNSSFITEDHAAAFVRNHFAMPGAEEPIDKALRLDSTIMLVDDPVKRVDNMTMAWGLEARVPFLDHE
LVELAAKIPAEYKVKDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGEYLDMAKNILNQDAAKQRKIFNREYVDM
LLNAPQDHITPLRGSKLWQITLLEYWLQEQGL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=555, Percent_Identity=27.9279279279279, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI168229252, Length=555, Percent_Identity=27.9279279279279, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI168229250, Length=555, Percent_Identity=27.9279279279279, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI296010848, Length=526, Percent_Identity=27.9467680608365, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI296010852, Length=459, Percent_Identity=27.4509803921569, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI296010850, Length=459, Percent_Identity=27.4509803921569, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI1786889, Length=576, Percent_Identity=26.5625, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147557, Length=534, Percent_Identity=27.9026217228464, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71993933, Length=534, Percent_Identity=27.9026217228464, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17560178, Length=395, Percent_Identity=26.0759493670886, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=575, Percent_Identity=26.9565217391304, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325403, Length=583, Percent_Identity=27.2727272727273, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45553209, Length=521, Percent_Identity=26.679462571977, Blast_Score=145, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 66828; Mature: 66828

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGELRFDSAAASSTTVDAMLGILEPRGPDAQGVFAHNRICFGHRRLKIIDLSDCAS
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCEEECCCEEEEEECHHHCC
QPMVDSALGLVLVFNGAVYNYRELCTELVGKGYSFFSQSDTEIIIKAYHAWGEDCVGHLQ
CCCHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
GMFAFAIWERDTGRTFFARDRLGIKPLYFTEDNASMRFASSLPALLKSGAVDTAIDPAAL
HHHHHEEEECCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
DCYMSFHSVVPAPWTIFRGIRKLPPATTMMVEPDGRKQQRTYWQPDYSRSDEERRRSAGE
HHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
WIDAVHDALKLAVQRRLVADVPVGVLLSGGLDSSLVVGLLSELVQRHLSTFSVGFEDVAE
HHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EEGNEFRYSDIIAERYSTDHHKIFVDHRELQTHLSDCVRAMSEPMVSHDVIGFYLLSREV
HCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SQHVRVVQSGQGADEVFAGYHWYPPMMNTPPTEAYSTYSGVFFDRDFNEYRQAVNSSFIT
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCEEEECCHHHHHHHHHHHCCC
EDHAAAFVRNHFAMPGAEEPIDKALRLDSTIMLVDDPVKRVDNMTMAWGLEARVPFLDHE
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCHHHHHCCCEEEECCCCCCCCHHHH
LVELAAKIPAEYKVKDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGEYLDMAKN
HHHHHHHCCCCEEECCCCHHHHHHHCHHCCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHH
ILNQDAAKQRKIFNREYVDMLLNAPQDHITPLRGSKLWQITLLEYWLQEQGL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGELRFDSAAASSTTVDAMLGILEPRGPDAQGVFAHNRICFGHRRLKIIDLSDCAS
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCEEECCCEEEEEECHHHCC
QPMVDSALGLVLVFNGAVYNYRELCTELVGKGYSFFSQSDTEIIIKAYHAWGEDCVGHLQ
CCCHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHH
GMFAFAIWERDTGRTFFARDRLGIKPLYFTEDNASMRFASSLPALLKSGAVDTAIDPAAL
HHHHHEEEECCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
DCYMSFHSVVPAPWTIFRGIRKLPPATTMMVEPDGRKQQRTYWQPDYSRSDEERRRSAGE
HHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
WIDAVHDALKLAVQRRLVADVPVGVLLSGGLDSSLVVGLLSELVQRHLSTFSVGFEDVAE
HHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EEGNEFRYSDIIAERYSTDHHKIFVDHRELQTHLSDCVRAMSEPMVSHDVIGFYLLSREV
HCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SQHVRVVQSGQGADEVFAGYHWYPPMMNTPPTEAYSTYSGVFFDRDFNEYRQAVNSSFIT
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCEEEECCHHHHHHHHHHHCCC
EDHAAAFVRNHFAMPGAEEPIDKALRLDSTIMLVDDPVKRVDNMTMAWGLEARVPFLDHE
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCHHHHHCCCEEEECCCCCCCCHHHH
LVELAAKIPAEYKVKDGGKYVLKEVGRRIIPHEVIDRPKGYFPVPALKYLEGEYLDMAKN
HHHHHHHCCCCEEECCCCHHHHHHHCHHCCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHH
ILNQDAAKQRKIFNREYVDMLLNAPQDHITPLRGSKLWQITLLEYWLQEQGL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]