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The map label for this gene is carA [H]

Identifier: 193213612

GI number: 193213612

Start: 2178711

End: 2179811

Strand: Direct

Name: carA [H]

Synonym: Cpar_1976

Alternate gene names: 193213612

Gene position: 2178711-2179811 (Clockwise)

Preceding gene: 193213611

Following gene: 193213613

Centisome position: 95.17

GC content: 59.76

Gene sequence:

>1101_bases
ATGCAGGAAACACCCGCAATTCTGGTTCTCGAAAACGGTTCGATCTATCGCGGAACCGCCTTCGGGCACACCTCCGAGGC
CTCCGGTGAGGTGGTCTTCAACACTTCGCTGACCGGGTATCAGGAGATTCTTACCGATCCGTCCTACGCCGGGCAGATGG
TCGTTATGACCTACCCGCTCATCGGCAACTACGGCATCACGCCGAACGATAACGAGTCATCAAAAATCTGGGCATCAGCG
CTCATCGTGCGCGAAGCCTCGAACGTCTACAGCAACTTCGAATCGACCCGCAGCCTCGACGAAACGCTCAAAGAGGCCGG
GGTGATGGGTCTTGCTGGCATCGATACCCGCAAGCTGGTACGGGAGATTCGCGAGAAGGGCGCCATGCGCGGCGTCATCT
CAACCCTGACCGACGATGTCGAGGCGCTGAAAGCAAAAGCGCTCGCCATCCCCGAAATGACCGGTCTCGATCTGGTGAGC
ACCGTCACGACCGACGCGAGCTACACGGTGGACAACGAAGAGGCGAGCTACCACGTCGTCGCTTTCGACTACGGCATCAA
GACCAACATCCTTCGCCAGCTCAAAGCCGAGGGATGCCGCGTCACGGTGGTCACCGCTAAAACCACCGCCGAAGAGGTAC
TCGCACTGAATCCCGACGGGGTCTTCCTCTCCAACGGCCCCGGCGATCCGTTCGCCGTAACCTACGCCATCGACACCATC
CGAGAGCTGGCCGCTCGCAACAGCAAGCTGCCGATCTTCGGCATCTGCCTCGGCCACCAGCTGCTGTCGCTGGCCTTTGG
CGCGAAGACCTACAAGCTCAAGTTCGGCCACCACGGCGCGAACCATCCGGTCAAGAACCTCATGAACAGCACCATCGAAA
TCACCTCGCAGAACCACGGGTTTGCGGTCGAGATGGACACGCTTCCCGGCGACCTTGAACTGACCCACAAGAACCTCTAC
GACCTCACCGTCGAGGGAGTCAGGCATCGGGAGTTACCCTGCTTCTCGGTGCAATATCACCCCGAAGCCGCCCCCGGCCC
GCACGACTCGCACTACCTGTTCAAGGAGTTCACCGAACTGATGGACGCTGCAAAAAACTGA

Upstream 100 bases:

>100_bases
TGCCGATAATCTCAAGATCAAGTTCGAGCGTTCGGCAATCGCCAACACTGAAAAACAGGAAACCGGCGACAAGCTCGCCC
CAAAGGAGTAACAGGAATCG

Downstream 100 bases:

>100_bases
CCTGTACTTACCGGTGGGGATTCCATCCAGACAATCCCCACCGGTAAATCCCCCATATTCTTCTACGAACGCCCGGTTCC
GCATTTCAAGAGATTATTTT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQETPAILVLENGSIYRGTAFGHTSEASGEVVFNTSLTGYQEILTDPSYAGQMVVMTYPLIGNYGITPNDNESSKIWASA
LIVREASNVYSNFESTRSLDETLKEAGVMGLAGIDTRKLVREIREKGAMRGVISTLTDDVEALKAKALAIPEMTGLDLVS
TVTTDASYTVDNEEASYHVVAFDYGIKTNILRQLKAEGCRVTVVTAKTTAEEVLALNPDGVFLSNGPGDPFAVTYAIDTI
RELAARNSKLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLMNSTIEITSQNHGFAVEMDTLPGDLELTHKNLY
DLTVEGVRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTELMDAAKN

Sequences:

>Translated_366_residues
MQETPAILVLENGSIYRGTAFGHTSEASGEVVFNTSLTGYQEILTDPSYAGQMVVMTYPLIGNYGITPNDNESSKIWASA
LIVREASNVYSNFESTRSLDETLKEAGVMGLAGIDTRKLVREIREKGAMRGVISTLTDDVEALKAKALAIPEMTGLDLVS
TVTTDASYTVDNEEASYHVVAFDYGIKTNILRQLKAEGCRVTVVTAKTTAEEVLALNPDGVFLSNGPGDPFAVTYAIDTI
RELAARNSKLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLMNSTIEITSQNHGFAVEMDTLPGDLELTHKNLY
DLTVEGVRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTELMDAAKN
>Mature_366_residues
MQETPAILVLENGSIYRGTAFGHTSEASGEVVFNTSLTGYQEILTDPSYAGQMVVMTYPLIGNYGITPNDNESSKIWASA
LIVREASNVYSNFESTRSLDETLKEAGVMGLAGIDTRKLVREIREKGAMRGVISTLTDDVEALKAKALAIPEMTGLDLVS
TVTTDASYTVDNEEASYHVVAFDYGIKTNILRQLKAEGCRVTVVTAKTTAEEVLALNPDGVFLSNGPGDPFAVTYAIDTI
RELAARNSKLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLMNSTIEITSQNHGFAVEMDTLPGDLELTHKNLY
DLTVEGVRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTELMDAAKN

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=368, Percent_Identity=41.5760869565217, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI169790915, Length=371, Percent_Identity=37.1967654986523, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI21361331, Length=371, Percent_Identity=37.1967654986523, Blast_Score=235, Evalue=5e-62,
Organism=Escherichia coli, GI1786215, Length=373, Percent_Identity=50.6702412868633, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1789760, Length=143, Percent_Identity=32.8671328671329, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=373, Percent_Identity=41.2868632707775, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324878, Length=371, Percent_Identity=40.1617250673854, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=400, Percent_Identity=35.75, Blast_Score=235, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6322638, Length=178, Percent_Identity=32.0224719101124, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45555749, Length=379, Percent_Identity=37.2031662269129, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24642586, Length=379, Percent_Identity=37.2031662269129, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39924; Mature: 39924

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETPAILVLENGSIYRGTAFGHTSEASGEVVFNTSLTGYQEILTDPSYAGQMVVMTYPL
CCCCCEEEEEECCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEECE
IGNYGITPNDNESSKIWASALIVREASNVYSNFESTRSLDETLKEAGVMGLAGIDTRKLV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHH
REIREKGAMRGVISTLTDDVEALKAKALAIPEMTGLDLVSTVTTDASYTVDNEEASYHVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCEEEECCCCCCEEEE
AFDYGIKTNILRQLKAEGCRVTVVTAKTTAEEVLALNPDGVFLSNGPGDPFAVTYAIDTI
EEECCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCEEEECCCCCCEEEEHHHHHH
RELAARNSKLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLMNSTIEITSQNHG
HHHHHCCCCCCEEEEHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHCCCEEEEEECCCC
FAVEMDTLPGDLELTHKNLYDLTVEGVRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTEL
EEEEECCCCCCEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
MDAAKN
HHHHCC
>Mature Secondary Structure
MQETPAILVLENGSIYRGTAFGHTSEASGEVVFNTSLTGYQEILTDPSYAGQMVVMTYPL
CCCCCEEEEEECCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEECE
IGNYGITPNDNESSKIWASALIVREASNVYSNFESTRSLDETLKEAGVMGLAGIDTRKLV
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHH
REIREKGAMRGVISTLTDDVEALKAKALAIPEMTGLDLVSTVTTDASYTVDNEEASYHVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCEEEECCCCCCEEEE
AFDYGIKTNILRQLKAEGCRVTVVTAKTTAEEVLALNPDGVFLSNGPGDPFAVTYAIDTI
EEECCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCEEEECCCCCCEEEEHHHHHH
RELAARNSKLPIFGICLGHQLLSLAFGAKTYKLKFGHHGANHPVKNLMNSTIEITSQNHG
HHHHHCCCCCCEEEEHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHCCCEEEEEECCCC
FAVEMDTLPGDLELTHKNLYDLTVEGVRHRELPCFSVQYHPEAAPGPHDSHYLFKEFTEL
EEEEECCCCCCEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
MDAAKN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]