Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 193213362

GI number: 193213362

Start: 1884651

End: 1886144

Strand: Direct

Name: purF [H]

Synonym: Cpar_1723

Alternate gene names: 193213362

Gene position: 1884651-1886144 (Clockwise)

Preceding gene: 193213359

Following gene: 193213372

Centisome position: 82.33

GC content: 55.82

Gene sequence:

>1494_bases
ATGTGCGGAGTTTTCGGCATTTTCAACTCAAAAACTCCCGCCGAAGATACCTTTTACGGCCTCTACTCGCTACAACACCG
GGGACAGGAAGCAGCAGGTATCGTAGTTGCCGAATACAACAAGGCCAAGAAAAAGACCCTGTTCAAGCAGCATAAAGGGC
CGGGACTGGTATCCGAAGTTTTCAGGGACGAACAGATTTTCGAGAACCTGAGCGGATACGCAGCAATCGGCCATAACCGC
TACTCCACCACCGGCGCCTCCAAATCGAACAACAACATCCAGCCTTTCTCCCTCACCTACCGCTCCGGCAGCCTCGCCAT
CGCCCACAACGGAAACCTGACCAATTCGAGAGTTTTGCGCAAGGAGCTGACCGAAGAGGGCGTGATTTTCCAGGCCTCAT
CGGATACCGAAATCATCCCGCACCTTGCTGCGCGCAGCAAGGAAAAAGAGCCACTGCACCAGATTTACGATGCATTGCGG
CAGGTAGAAGGAGCTTTTTCGATCGTGATTCTGGCCAACAACCAGATGATTGCGGCGCGAGACCCTTACGGCGTGCGCCC
CTTGGCTCTCGGCAAGAAAATCGATCCCGCAACCGGTGAGCTGGCCTACGTGGTAGCAAGCGAAACCTGTGCTTTCGACA
TCATCAAGGCTGAGTACATCCGCGACATCGCACCGGGAGAGATTCTCTTGATCGACCACCTCTCAATAGAAAACGAAAAG
CCGATCTCGCTGTTCCTGCCGCCGGTCGAGCGCAAGGCTCGCTGCATCTTCGAGTATGTCTATTTCGCCCGCCCGGACAG
CTTCATCTTTCGGCACTCGGTTGACAAGGTGCGCAGAAACCTCGGCAAGAACCTGGCCAGGGAATCAACGATCGAACGAG
ACCCCAACGACAAGGAGCTGGCCGTAGTCAGCGTGCCGGACTCATCGAACACGGCTGCGCTCGGCTTCGTCAGAGAAAGC
AACAAACTCGGCAAGCCTGCGCGCTTCGAGCACGGCCTGATCCGCAACCACTATGTTGGCCGCACCTTTATTCAGCCGGG
CATTCAAAGCCGTGAAATCAAGGTTCGCTCGAAATACAATATCGTGCGCGGCGTCATGCAGGGCCGTCCGATCATCCTCG
TCGACGACTCGATCGTGCGCGGCACGACAGCCAAGATGCTCATCAAGCTCGTTCGCGAAGCCAATCCGAAAGAGATTCAT
CTGCACATCAGCTCGCCACCGATCACCAATCCCTGCTTTTACGGCATGGACTTCCCCTCCAAGCGACAGTTGCTGACCCA
CATGCTTGCCGACGCGGAGGACGAGCTGGGAAATATTGAAAAAATCAGGGACTACATCGGTGTGGATTCACTCCGCTACC
TGACCATGCAGGGGCTTCTGAACAGCGTGCCGGAATTCGAGGGCGAAAAGTGCAGCTACTGCACTGCCTGCTTCAGCGGC
GACTACCCTATCCCGATTACCGACGCCACGACCGACAAAGAGGAGAACGACTGA

Upstream 100 bases:

>100_bases
CCCTTTTTCTCTTTCATGGTTTTTATGTTTCCCCTTGATATATTAGCTTCACTTGCCGCTGGACGGTAAAGATTTTACAC
AATCTCAAGATTTGATCGAT

Downstream 100 bases:

>100_bases
TCACCTCCCCGGCAAAGCGCCACCTGAATTCAGCAGAAAAGCCGGTCGAATCCCAAAAGATTCGACCGGCTTTTTCCATT
CTTTAACGATCACCATCAGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGPGLVSEVFRDEQIFENLSGYAAIGHNR
YSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLRKELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALR
QVEGAFSIVILANNQMIAARDPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIAPGEILLIDHLSIENEK
PISLFLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKELAVVSVPDSSNTAALGFVRES
NKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYNIVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIH
LHISSPPITNPCFYGMDFPSKRQLLTHMLADAEDELGNIEKIRDYIGVDSLRYLTMQGLLNSVPEFEGEKCSYCTACFSG
DYPIPITDATTDKEEND

Sequences:

>Translated_497_residues
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGPGLVSEVFRDEQIFENLSGYAAIGHNR
YSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLRKELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALR
QVEGAFSIVILANNQMIAARDPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIAPGEILLIDHLSIENEK
PISLFLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKELAVVSVPDSSNTAALGFVRES
NKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYNIVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIH
LHISSPPITNPCFYGMDFPSKRQLLTHMLADAEDELGNIEKIRDYIGVDSLRYLTMQGLLNSVPEFEGEKCSYCTACFSG
DYPIPITDATTDKEEND
>Mature_497_residues
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGPGLVSEVFRDEQIFENLSGYAAIGHNR
YSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLRKELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALR
QVEGAFSIVILANNQMIAARDPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIAPGEILLIDHLSIENEK
PISLFLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKELAVVSVPDSSNTAALGFVRES
NKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYNIVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIH
LHISSPPITNPCFYGMDFPSKRQLLTHMLADAEDELGNIEKIRDYIGVDSLRYLTMQGLLNSVPEFEGEKCSYCTACFSG
DYPIPITDATTDKEEND

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=530, Percent_Identity=36.7924528301887, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI4826742, Length=189, Percent_Identity=27.5132275132275, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788651, Length=498, Percent_Identity=37.7510040160643, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790167, Length=198, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=505, Percent_Identity=36.2376237623762, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17532899, Length=217, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17532897, Length=217, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=32.0079522862823, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24659598, Length=508, Percent_Identity=39.5669291338583, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI24659604, Length=484, Percent_Identity=40.0826446280992, Blast_Score=322, Evalue=5e-88,
Organism=Drosophila melanogaster, GI28573187, Length=519, Percent_Identity=37.3795761078998, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI21357745, Length=185, Percent_Identity=26.4864864864865, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55560; Mature: 55560

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGPGLVSEV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCHHHHHHHHHHCCCCCHHHHH
FRDEQIFENLSGYAAIGHNRYSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLR
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHH
KELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALRQVEGAFSIVILANNQMIAAR
HHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEC
DPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIAPGEILLIDHLSIENEK
CCCCCCCHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
PISLFLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKEL
CEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AVVSVPDSSNTAALGFVRESNKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYN
EEEECCCCCCCEEEEEEECCCCCCCCCHHCCCCHHHCCCCHHHHCCCCCCCEEEEHHHHH
IVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIHLHISSPPITNPCFYGMDFPS
HHHHHHCCCCEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHMLADAEDELGNIEKIRDYIGVDSLRYLTMQGLLNSVPEFEGEKCSYCTACFSG
HHHHHHHHHHCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
DYPIPITDATTDKEEND
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGVFGIFNSKTPAEDTFYGLYSLQHRGQEAAGIVVAEYNKAKKKTLFKQHKGPGLVSEV
CCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCHHHHHHHHHHCCCCCHHHHH
FRDEQIFENLSGYAAIGHNRYSTTGASKSNNNIQPFSLTYRSGSLAIAHNGNLTNSRVLR
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHH
KELTEEGVIFQASSDTEIIPHLAARSKEKEPLHQIYDALRQVEGAFSIVILANNQMIAAR
HHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEC
DPYGVRPLALGKKIDPATGELAYVVASETCAFDIIKAEYIRDIAPGEILLIDHLSIENEK
CCCCCCCHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
PISLFLPPVERKARCIFEYVYFARPDSFIFRHSVDKVRRNLGKNLARESTIERDPNDKEL
CEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AVVSVPDSSNTAALGFVRESNKLGKPARFEHGLIRNHYVGRTFIQPGIQSREIKVRSKYN
EEEECCCCCCCEEEEEEECCCCCCCCCHHCCCCHHHCCCCHHHHCCCCCCCEEEEHHHHH
IVRGVMQGRPIILVDDSIVRGTTAKMLIKLVREANPKEIHLHISSPPITNPCFYGMDFPS
HHHHHHCCCCEEEECCCHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHMLADAEDELGNIEKIRDYIGVDSLRYLTMQGLLNSVPEFEGEKCSYCTACFSG
HHHHHHHHHHCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
DYPIPITDATTDKEEND
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]