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The map label for this gene is nfo

Identifier: 193213359

GI number: 193213359

Start: 1881886

End: 1882731

Strand: Direct

Name: nfo

Synonym: Cpar_1720

Alternate gene names: 193213359

Gene position: 1881886-1882731 (Clockwise)

Preceding gene: 193213358

Following gene: 193213362

Centisome position: 82.21

GC content: 60.52

Gene sequence:

>846_bases
ATGAAACGGGTCGGCGCCCATGTCAGTATTGCCGGAGGCGTCGAAAACGCCCCGCTGAACGCACAGAAAATCGGCGCGAA
AGCGTTCGCCATGTTCACTCGGAACCAACGCCAGTGGCACTCCGCACCGCTTTCGACGGCTTCGATCGAGGCATTCCGGC
GGAACTGCGAGGAGGCGGGATTCCTGCCCCAGCACATTTTGCCGCACGACAGCTACCTCATCAACCTCGGCGCTCCCGAA
GTTGAAAAGCTCGAAAAGTCGCGCAAGGCGTTCACCACCGAAATGCAGCGCGCCGAAGCGCTCGGCCTCACGATGCTGAA
CTTCCACCCCGGCAGCCATCTCAATCTCGTCAGTGAGAATGAGTGCCTCGCCACCATCGCCGAGTCGGTGAACCTGTCGC
TCAACGCGACGGCGGGCGTAACGGCGGTGATCGAAAACACGGCAGGACAAGGCAGTAATCTCGGCTGGCGCTTCGAGCAT
CTTGCGCGAATCATCGAGCTGGTCGAAGACAAATCGCGGGTCGGCGTCTGCCTCGACACCTGCCACCTGTTCGCCAGCGG
CTACGACCTGCGTACGCCCGAAGCGTTCGACGCCACGCTGGCGGAGTTCGACCGCGTGGTGGGCTTATCGTACCTGAGGG
GAATGCATCTGAACGATGCAAAACAGAAGCTTGGCAGCCGGGTTGACCGGCACGCCTGCATCGGAGAGGGAATGATCGGG
CGCGAGGCGTTCGTGCATCTCATGCAGCACCCGGCGATGGAGGAGATTCCGCTGATTCTCGAAACTCCGAACTCCGAAAG
CTGGGCAGAGGAGATCGAGATGCTTTACAGCTTCGAGAAAGAGTAA

Upstream 100 bases:

>100_bases
GCCTGACACCATCATCGACAAACTGAACGAGTGAACTTCCGGCAGCAGATCGCCGTTACGGTATGCTCGATTCACGAAAA
CATCCAAACCAGACGATAGT

Downstream 100 bases:

>100_bases
TTACGTCCGCCGCAGAAAGCGCGCCACCGAAAAGATGCTGCCAAAAACTCCGAGCACGAGGCCAAGCAGGGCCATCGCGG
GATAGACGTAGAGCGTGGAA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLSTASIEAFRRNCEEAGFLPQHILPHDSYLINLGAPE
VEKLEKSRKAFTTEMQRAEALGLTMLNFHPGSHLNLVSENECLATIAESVNLSLNATAGVTAVIENTAGQGSNLGWRFEH
LARIIELVEDKSRVGVCLDTCHLFASGYDLRTPEAFDATLAEFDRVVGLSYLRGMHLNDAKQKLGSRVDRHACIGEGMIG
REAFVHLMQHPAMEEIPLILETPNSESWAEEIEMLYSFEKE

Sequences:

>Translated_281_residues
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLSTASIEAFRRNCEEAGFLPQHILPHDSYLINLGAPE
VEKLEKSRKAFTTEMQRAEALGLTMLNFHPGSHLNLVSENECLATIAESVNLSLNATAGVTAVIENTAGQGSNLGWRFEH
LARIIELVEDKSRVGVCLDTCHLFASGYDLRTPEAFDATLAEFDRVVGLSYLRGMHLNDAKQKLGSRVDRHACIGEGMIG
REAFVHLMQHPAMEEIPLILETPNSESWAEEIEMLYSFEKE
>Mature_281_residues
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLSTASIEAFRRNCEEAGFLPQHILPHDSYLINLGAPE
VEKLEKSRKAFTTEMQRAEALGLTMLNFHPGSHLNLVSENECLATIAESVNLSLNATAGVTAVIENTAGQGSNLGWRFEH
LARIIELVEDKSRVGVCLDTCHLFASGYDLRTPEAFDATLAEFDRVVGLSYLRGMHLNDAKQKLGSRVDRHACIGEGMIG
REAFVHLMQHPAMEEIPLILETPNSESWAEEIEMLYSFEKE

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=281, Percent_Identity=63.3451957295374, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17531193, Length=279, Percent_Identity=48.0286738351254, Blast_Score=283, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6322735, Length=276, Percent_Identity=39.8550724637681, Blast_Score=225, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_CHLP8 (B3QQA9)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001999312.1
- ProteinModelPortal:   B3QQA9
- GeneID:   6420661
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_1720
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31070; Mature: 31070

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLSTASIEAFRRNCEEAG
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCC
FLPQHILPHDSYLINLGAPEVEKLEKSRKAFTTEMQRAEALGLTMLNFHPGSHLNLVSEN
CCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCC
ECLATIAESVNLSLNATAGVTAVIENTAGQGSNLGWRFEHLARIIELVEDKSRVGVCLDT
HHHHHHHHHHCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
CHLFASGYDLRTPEAFDATLAEFDRVVGLSYLRGMHLNDAKQKLGSRVDRHACIGEGMIG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCH
REAFVHLMQHPAMEEIPLILETPNSESWAEEIEMLYSFEKE
HHHHHHHHHCCCHHHCCEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLSTASIEAFRRNCEEAG
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCC
FLPQHILPHDSYLINLGAPEVEKLEKSRKAFTTEMQRAEALGLTMLNFHPGSHLNLVSEN
CCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCC
ECLATIAESVNLSLNATAGVTAVIENTAGQGSNLGWRFEHLARIIELVEDKSRVGVCLDT
HHHHHHHHHHCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
CHLFASGYDLRTPEAFDATLAEFDRVVGLSYLRGMHLNDAKQKLGSRVDRHACIGEGMIG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCH
REAFVHLMQHPAMEEIPLILETPNSESWAEEIEMLYSFEKE
HHHHHHHHHCCCHHHCCEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA