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The map label for this gene is queA [H]

Identifier: 193212984

GI number: 193212984

Start: 1449415

End: 1450455

Strand: Direct

Name: queA [H]

Synonym: Cpar_1334

Alternate gene names: 193212984

Gene position: 1449415-1450455 (Clockwise)

Preceding gene: 193212983

Following gene: 193212985

Centisome position: 63.31

GC content: 56.87

Gene sequence:

>1041_bases
ATGCGGCTCTCGAACTTTCGATATACCCTCCCCAAAACACGAATCGCCGACCACCCCGAATCGCCCCGCGACGCCTGCAA
GCTGATGGTGCTGAACCGGCGCAAAAAGGACATCGAGCACAAGGTTTTCACCGACATTGTCTCGTACTTCAAAAAAGGCG
ACCTGCTGGTGGTCAACAACAGCCGGGTTTTTCCGGCCAAAATCTTCGGCCAGAAAGAGAAAACCGACGCCAAGATCGAG
GTCTTTCTGCTCCGCGAGCTGAACAAAGAGGCCGGGCTCTGGGACGTGCTGGTTGATCCTGCGCGCAAGGTTCGCGTCGG
CAATAAAATCTATTTCGAGGACGACGTTGTGGCCGAAGTAGTAGACAACACCACCTCGAGAGGCCGGACGATCCGCTTCC
TCAACCCCGATATCGACGTCTTCAAGATGGTTGAAAAAATCGGCCAGGTACCGCTGCCACCCTACTTCACCAGAAAGCCA
AAGGATTCGGACCGGACCGACTACCAGACCGTCTACGCCAGCCAGACCGGTGCCGTGGTCGCCCCGATGGGCGGGCTGCA
CTTCACCATGCCGCTACTGGAACAGATCCAGAAAGTGGGCGTAAAGATTCTGCCCATCACCCTGCACCCGAGCCTGAGCA
CCTTCAACGCCATCGAGGTTGAAGATGTATCGAAGCACAAGATGGACTCCGAGTACTTCAACATCCCCTACCAGACGGCC
ATGGAGATCAACGAAACCAAGGTCAACAAGTCCGGACGGGTGTTCGTCGTCGGCACCACGACCTGCCGCGCACTGGAAGC
CAATGCCACCGTGGAGGGCAAGATCAAATTCGGCCAGGGATGGACCGACAAGTTCATCTATCCGCCCTACAACTTCAAGG
TCACCGACGCGCTCATCACCAACTTCCAGCAGCCCGAAACGACCCTGATGATGGTGGTCAGCGCTTTCGCCGAGCACCGC
CTGCTCATGGACGCCTACAAAACCGCACTCAAGAACAACTACCGGTTCCTGGCCTACGGGGACTCAATGCTCATCGTCTG
A

Upstream 100 bases:

>100_bases
GGAGCCCCAACCCTCCTCCGGATTTTGTATTTCACGCGGCACGCCCTATATTTAAGTTCGCCTATTCAGGGCTCATCACT
CACAGACCCCCAATAACGAT

Downstream 100 bases:

>100_bases
AGACCATGAAAACCGTTGCAATCATCGCCGCCAGCGGAGTCGGCAAACGCATGAAGCTCGATGGCGGCATGAGCAAGCAG
ATGATCGAAATCGGAGGCCA

Product: S-adenosylmethionine/tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRLSNFRYTLPKTRIADHPESPRDACKLMVLNRRKKDIEHKVFTDIVSYFKKGDLLVVNNSRVFPAKIFGQKEKTDAKIE
VFLLRELNKEAGLWDVLVDPARKVRVGNKIYFEDDVVAEVVDNTTSRGRTIRFLNPDIDVFKMVEKIGQVPLPPYFTRKP
KDSDRTDYQTVYASQTGAVVAPMGGLHFTMPLLEQIQKVGVKILPITLHPSLSTFNAIEVEDVSKHKMDSEYFNIPYQTA
MEINETKVNKSGRVFVVGTTTCRALEANATVEGKIKFGQGWTDKFIYPPYNFKVTDALITNFQQPETTLMMVVSAFAEHR
LLMDAYKTALKNNYRFLAYGDSMLIV

Sequences:

>Translated_346_residues
MRLSNFRYTLPKTRIADHPESPRDACKLMVLNRRKKDIEHKVFTDIVSYFKKGDLLVVNNSRVFPAKIFGQKEKTDAKIE
VFLLRELNKEAGLWDVLVDPARKVRVGNKIYFEDDVVAEVVDNTTSRGRTIRFLNPDIDVFKMVEKIGQVPLPPYFTRKP
KDSDRTDYQTVYASQTGAVVAPMGGLHFTMPLLEQIQKVGVKILPITLHPSLSTFNAIEVEDVSKHKMDSEYFNIPYQTA
MEINETKVNKSGRVFVVGTTTCRALEANATVEGKIKFGQGWTDKFIYPPYNFKVTDALITNFQQPETTLMMVVSAFAEHR
LLMDAYKTALKNNYRFLAYGDSMLIV
>Mature_346_residues
MRLSNFRYTLPKTRIADHPESPRDACKLMVLNRRKKDIEHKVFTDIVSYFKKGDLLVVNNSRVFPAKIFGQKEKTDAKIE
VFLLRELNKEAGLWDVLVDPARKVRVGNKIYFEDDVVAEVVDNTTSRGRTIRFLNPDIDVFKMVEKIGQVPLPPYFTRKP
KDSDRTDYQTVYASQTGAVVAPMGGLHFTMPLLEQIQKVGVKILPITLHPSLSTFNAIEVEDVSKHKMDSEYFNIPYQTA
MEINETKVNKSGRVFVVGTTTCRALEANATVEGKIKFGQGWTDKFIYPPYNFKVTDALITNFQQPETTLMMVVSAFAEHR
LLMDAYKTALKNNYRFLAYGDSMLIV

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=39.1428571428571, Blast_Score=247, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39524; Mature: 39524

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSNFRYTLPKTRIADHPESPRDACKLMVLNRRKKDIEHKVFTDIVSYFKKGDLLVVNN
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEC
SRVFPAKIFGQKEKTDAKIEVFLLRELNKEAGLWDVLVDPARKVRVGNKIYFEDDVVAEV
CEECCHHHCCCCCCCCCEEEEEEEECCCCCCCEEHHHHCCHHHEECCCEEEECCHHHHHH
VDNTTSRGRTIRFLNPDIDVFKMVEKIGQVPLPPYFTRKPKDSDRTDYQTVYASQTGAVV
HHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
APMGGLHFTMPLLEQIQKVGVKILPITLHPSLSTFNAIEVEDVSKHKMDSEYFNIPYQTA
ECCCCCEEHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEECHHHHHCCCCHHCCCCHHHH
MEINETKVNKSGRVFVVGTTTCRALEANATVEGKIKFGQGWTDKFIYPPYNFKVTDALIT
EEECHHEECCCCCEEEEECCEEEEEECCCEEEEEEEECCCCCCCEEECCCCCEEHHHHHC
NFQQPETTLMMVVSAFAEHRLLMDAYKTALKNNYRFLAYGDSMLIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEC
>Mature Secondary Structure
MRLSNFRYTLPKTRIADHPESPRDACKLMVLNRRKKDIEHKVFTDIVSYFKKGDLLVVNN
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEC
SRVFPAKIFGQKEKTDAKIEVFLLRELNKEAGLWDVLVDPARKVRVGNKIYFEDDVVAEV
CEECCHHHCCCCCCCCCEEEEEEEECCCCCCCEEHHHHCCHHHEECCCEEEECCHHHHHH
VDNTTSRGRTIRFLNPDIDVFKMVEKIGQVPLPPYFTRKPKDSDRTDYQTVYASQTGAVV
HHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
APMGGLHFTMPLLEQIQKVGVKILPITLHPSLSTFNAIEVEDVSKHKMDSEYFNIPYQTA
ECCCCCEEHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEECHHHHHCCCCHHCCCCHHHH
MEINETKVNKSGRVFVVGTTTCRALEANATVEGKIKFGQGWTDKFIYPPYNFKVTDALIT
EEECHHEECCCCCEEEEECCEEEEEECCCEEEEEEEECCCCCCCEEECCCCCEEHHHHHC
NFQQPETTLMMVVSAFAEHRLLMDAYKTALKNNYRFLAYGDSMLIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA