BacMap

Definition	Rhodopseudomonas palustris TIE-1 chromosome, complete genome.
Accession	NC_011004
Length	5,744,041

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The map label for this gene is fadB1 [H]

Identifier: 192289147

GI number: 192289147

Start: 751984

End: 752760

Strand: Reverse

Name: fadB1 [H]

Synonym: Rpal_0717

Alternate gene names: 192289147

Gene position: 752760-751984 (Counterclockwise)

Preceding gene: 192289148

Following gene: 192289135

Centisome position: 13.11

GC content: 67.05

Gene sequence:

>777_bases
TTGTCGTCCAATCCTATCCTCACCGAAACCCAGGGCCGGGTCGGCATCATCACGCTGAACCGGCCGGATGTGCTCAACGC
GCTCAACGATGCCCTGATGGATGCGCTCGGCGGCGCGCTGCTGGCGTTCGACGCCGACGACGGCATCGGCGCGATCGTGA
TCGCCGGCAATACCCGGGCCTTCGCGGCCGGCGCCGACATCGCCTCGATGGCAGCATGGAGCTATAGCGACGTCTATGGC
TCGAACTTCATCACCCGCAACTGGGAAACGATCCGCCAAGTGCGGAAGCCAGTGCTGGCCGCGGTTGCCGGCCTCGCCTA
TGGCGGCGGCTGCGAGCTGGCACTGGCCTGCGATATCGTGATCGCTGGCCGCAGTGCCAAGTTCGCGCTGCCCGAAATCA
AGCTCGGCCTGCTGCCCGGCGCCGGTGGCACGCAACGGCTGCCGCGCGCGATCGGTAAAGCCAAGGCGATGGACATGTGC
CTGTCGGCGCGGCCGCTCAACGCCGAAGAGGCCGATCGCTATGGTCTGGTCTCCCGCGTCGTCGATGACGACAGGCTGCG
TGACGAGACCGTCGCGCTGGCCACCACGATCGCGGCGTTCTCCGCTCCGGCGCTGATGGTGCTGAAGGAAAGCCTCAATC
GCGCATTCGAGAGCACGCTCGCCGAAGGCATCCTGTTCGAGCGCCGTGAACTGCACGCCCGCTTCGCCAGCGCCGACGCT
CGCGAGGGGATTCAGGCCTTCCTGGAGAAGCGCGCACCGCGCTTCTCCCACCGCTAG

Upstream 100 bases:

>100_bases
GATGCCGGCGACGCCGTCCGGCAAGATCCAGAAATTCCGGCTGCGCGAGATGCTGCAGCACAACGATCTGTAAAGCGCAC
ACCGATCACGGAGCACGACC

Downstream 100 bases:

>100_bases
CGTGCGCGGCGCTGCCTCGTCATTTTAGATAGCTGGCAAGATTGAGGCTGAAGATCTTGCCGATCGCCGAGTACGACGCG
GTCAATTGCGACTGGTAGGT

Product: Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSSNPILTETQGRVGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAGNTRAFAAGADIASMAAWSYSDVYG
SNFITRNWETIRQVRKPVLAAVAGLAYGGGCELALACDIVIAGRSAKFALPEIKLGLLPGAGGTQRLPRAIGKAKAMDMC
LSARPLNAEEADRYGLVSRVVDDDRLRDETVALATTIAAFSAPALMVLKESLNRAFESTLAEGILFERRELHARFASADA
REGIQAFLEKRAPRFSHR

Sequences:

>Translated_258_residues
MSSNPILTETQGRVGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAGNTRAFAAGADIASMAAWSYSDVYG
SNFITRNWETIRQVRKPVLAAVAGLAYGGGCELALACDIVIAGRSAKFALPEIKLGLLPGAGGTQRLPRAIGKAKAMDMC
LSARPLNAEEADRYGLVSRVVDDDRLRDETVALATTIAAFSAPALMVLKESLNRAFESTLAEGILFERRELHARFASADA
REGIQAFLEKRAPRFSHR
>Mature_257_residues
SSNPILTETQGRVGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAGNTRAFAAGADIASMAAWSYSDVYGS
NFITRNWETIRQVRKPVLAAVAGLAYGGGCELALACDIVIAGRSAKFALPEIKLGLLPGAGGTQRLPRAIGKAKAMDMCL
SARPLNAEEADRYGLVSRVVDDDRLRDETVALATTIAAFSAPALMVLKESLNRAFESTLAEGILFERRELHARFASADAR
EGIQAFLEKRAPRFSHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=48.046875, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=31.9672131147541, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=36.8217054263566, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI68989263, Length=244, Percent_Identity=32.7868852459016, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=31.9391634980989, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI20127408, Length=192, Percent_Identity=36.4583333333333, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI37594471, Length=223, Percent_Identity=29.5964125560538, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594469, Length=223, Percent_Identity=29.5964125560538, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI261878539, Length=147, Percent_Identity=34.0136054421769, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=30.7377049180328, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI213417737, Length=188, Percent_Identity=27.1276595744681, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694516, Length=188, Percent_Identity=27.1276595744681, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=260, Percent_Identity=25.3846153846154, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=260, Percent_Identity=25.3846153846154, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=260, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI51538573, Length=260, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=26.0700389105058, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI116235487, Length=228, Percent_Identity=28.5087719298246, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=26.0700389105058, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=26.0700389105058, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4757966, Length=215, Percent_Identity=26.9767441860465, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI51538581, Length=215, Percent_Identity=26.9767441860465, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136756, Length=258, Percent_Identity=25.968992248062, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI221136753, Length=258, Percent_Identity=25.968992248062, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI221307494, Length=182, Percent_Identity=30.2197802197802, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=44.3137254901961, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=38.6973180076628, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=35.9848484848485, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=29.9242424242424, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=33.0677290836653, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=29.8342541436464, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=32.3232323232323, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=47.7551020408163, Blast_Score=234, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=47.7366255144033, Blast_Score=223, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17540306, Length=235, Percent_Identity=34.8936170212766, Blast_Score=134, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17560910, Length=264, Percent_Identity=32.1969696969697, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=218, Percent_Identity=30.7339449541284, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=35.9116022099448, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=37.3626373626374, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=181, Percent_Identity=37.5690607734807, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=181, Percent_Identity=37.5690607734807, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=26.7716535433071, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=271, Percent_Identity=26.5682656826568, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=252, Percent_Identity=25.7936507936508, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=192, Percent_Identity=28.125, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=192, Percent_Identity=28.125, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=184, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=235, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=28.8659793814433, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=50, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=50, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=32.2709163346614, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=26.953125, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=28.6259541984733, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650670, Length=206, Percent_Identity=32.5242718446602, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=29.1479820627803, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24654903, Length=253, Percent_Identity=24.901185770751, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921000, Length=198, Percent_Identity=32.8282828282828, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583077, Length=198, Percent_Identity=32.8282828282828, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583079, Length=198, Percent_Identity=32.8282828282828, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571730, Length=242, Percent_Identity=26.0330578512397, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28571729, Length=242, Percent_Identity=26.0330578512397, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27448; Mature: 27317

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNPILTETQGRVGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAGNTRA
CCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCEE
FAAGADIASMAAWSYSDVYGSNFITRNWETIRQVRKPVLAAVAGLAYGGGCELALACDIV
EECCCCHHHHHHCCHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
IAGRSAKFALPEIKLGLLPGAGGTQRLPRAIGKAKAMDMCLSARPLNAEEADRYGLVSRV
EECCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VDDDRLRDETVALATTIAAFSAPALMVLKESLNRAFESTLAEGILFERRELHARFASADA
HCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
REGIQAFLEKRAPRFSHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSNPILTETQGRVGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAGNTRA
CCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCEE
FAAGADIASMAAWSYSDVYGSNFITRNWETIRQVRKPVLAAVAGLAYGGGCELALACDIV
EECCCCHHHHHHCCHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
IAGRSAKFALPEIKLGLLPGAGGTQRLPRAIGKAKAMDMCLSARPLNAEEADRYGLVSRV
EECCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VDDDRLRDETVALATTIAAFSAPALMVLKESLNRAFESTLAEGILFERRELHARFASADA
HCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
REGIQAFLEKRAPRFSHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]