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The map label for this gene is dapE [H]

Identifier: 192289058

GI number: 192289058

Start: 682049

End: 683212

Strand: Reverse

Name: dapE [H]

Synonym: Rpal_0628

Alternate gene names: 192289058

Gene position: 683212-682049 (Counterclockwise)

Preceding gene: 192289059

Following gene: 192289054

Centisome position: 11.89

GC content: 65.98

Gene sequence:

>1164_bases
ATGACCTCGACTCCGACTGCGCTTTCGATCGCCCAAGACCTGCTGCGCTGCCCGTCTGTGACTCCGGCGGACGCCGGCGC
ACTCGACGTGCTGGAGACGCTGCTCAAAGGGGCGGGCTTCACGGTGCATCGCGTCACCTTCTCAGAGCCGGGCACCGCCG
ACATCGACAACCTGTATGCGCGGATCGGCAACACCTCGCCGCATCTTTGCTTCGCCGGCCACACCGACGTGGTGCCGCCG
GGCGATGCCAGTGCCTGGACCCACGGCGCGTTCGCCGGCGACGTTGCGGACGGCCTGCTGTACGGTCGCGGTGCGGTCGA
CATGAAAGGTGGTATCGCCTGCGCGGTCGCGGCAACGCTGGACTACCTCGCGGCAAACGGCGGCCAGCCCAAGGGCTCGA
TCTCGTTCCTGATCACCGGCGACGAGGAAGACGTCGCGGTCAACGGCACCGTCAAGCTGCTGCAATGGGCCGCCGAGCGC
GGCGAGCAATTCGATCACTGCATCGTCGGTGAGCCGAGCAATGTCGAGACGATCGGCGACACCATCAAGATCGGCCGGCG
CGGCTCGCAATCCGGCGTGCTGATCGTCGACGGTGTGCAGGGCCACGTCGCCTATCCGCACCGCGCCGCCAATCCGGTGC
CGGATATCGCCCGGCTGATCACCACGCTGAACGACGAGCCGCTCGATCACGGCTCGGCGCAATTCCAGCCGTCCAACCTG
GAATTCACCTCGGTCGATGTCGGCAATCCGGCCACCAACGTGATCCCGGCGCAAGCGCGCGCCAAATTCAACATCCGCTT
CAACGATCACCACACTCAGGAAACGCTGAAGGCACTGGTCGAGCAGCGGCTGGCTGCGGCGTGTGGCAACCGCATCCGCG
CCCACATCGAATGGCTGCCGTCGAACGCCGATGTGTTCGTCACCAAGCCCGGCGCCTTCACCGATCTGGTCGGCGCCGCG
ATCGCCGAGGTCACCGGACGCACGCCCGAGCTCAACACCGGCGGCGGCACATCCGACGCTCGCTTCATCGCGAAATATTG
CCAGGTGGTCGAATTCGGCTTGGTCGGCCAGACCATGCACCAGATCGACGAGCGCACGCCGGTCAGCGACCTCGACAAAC
TCACCGCGATCTACCGCGGCGTGCTGCAGAGATATTTCGCCTGA

Upstream 100 bases:

>100_bases
TCGCGATCTGGGGCTTCGTCGAACTCGGCTGCCTGCGCGGCACCCCCGGTCCTAACCAATACGGCCCCGACCCGTTGGAG
CCGCAAGGCACGCTGACCTG

Downstream 100 bases:

>100_bases
CTCTCCCCGTCATTCCGGGGCGCGAGCACAGCTCGCGAAACCGGAATCCGAGATTCAGGGCACCACTGAATTCGCGACAA
CATCGGGATTCCGGGTTCGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTSTPTALSIAQDLLRCPSVTPADAGALDVLETLLKGAGFTVHRVTFSEPGTADIDNLYARIGNTSPHLCFAGHTDVVPP
GDASAWTHGAFAGDVADGLLYGRGAVDMKGGIACAVAATLDYLAANGGQPKGSISFLITGDEEDVAVNGTVKLLQWAAER
GEQFDHCIVGEPSNVETIGDTIKIGRRGSQSGVLIVDGVQGHVAYPHRAANPVPDIARLITTLNDEPLDHGSAQFQPSNL
EFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLKALVEQRLAAACGNRIRAHIEWLPSNADVFVTKPGAFTDLVGAA
IAEVTGRTPELNTGGGTSDARFIAKYCQVVEFGLVGQTMHQIDERTPVSDLDKLTAIYRGVLQRYFA

Sequences:

>Translated_387_residues
MTSTPTALSIAQDLLRCPSVTPADAGALDVLETLLKGAGFTVHRVTFSEPGTADIDNLYARIGNTSPHLCFAGHTDVVPP
GDASAWTHGAFAGDVADGLLYGRGAVDMKGGIACAVAATLDYLAANGGQPKGSISFLITGDEEDVAVNGTVKLLQWAAER
GEQFDHCIVGEPSNVETIGDTIKIGRRGSQSGVLIVDGVQGHVAYPHRAANPVPDIARLITTLNDEPLDHGSAQFQPSNL
EFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLKALVEQRLAAACGNRIRAHIEWLPSNADVFVTKPGAFTDLVGAA
IAEVTGRTPELNTGGGTSDARFIAKYCQVVEFGLVGQTMHQIDERTPVSDLDKLTAIYRGVLQRYFA
>Mature_386_residues
TSTPTALSIAQDLLRCPSVTPADAGALDVLETLLKGAGFTVHRVTFSEPGTADIDNLYARIGNTSPHLCFAGHTDVVPPG
DASAWTHGAFAGDVADGLLYGRGAVDMKGGIACAVAATLDYLAANGGQPKGSISFLITGDEEDVAVNGTVKLLQWAAERG
EQFDHCIVGEPSNVETIGDTIKIGRRGSQSGVLIVDGVQGHVAYPHRAANPVPDIARLITTLNDEPLDHGSAQFQPSNLE
FTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLKALVEQRLAAACGNRIRAHIEWLPSNADVFVTKPGAFTDLVGAAI
AEVTGRTPELNTGGGTSDARFIAKYCQVVEFGLVGQTMHQIDERTPVSDLDKLTAIYRGVLQRYFA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=381, Percent_Identity=41.2073490813648, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1790395, Length=216, Percent_Identity=30.0925925925926, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40949; Mature: 40818

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTPTALSIAQDLLRCPSVTPADAGALDVLETLLKGAGFTVHRVTFSEPGTADIDNLYA
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
RIGNTSPHLCFAGHTDVVPPGDASAWTHGAFAGDVADGLLYGRGAVDMKGGIACAVAATL
HHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHH
DYLAANGGQPKGSISFLITGDEEDVAVNGTVKLLQWAAERGEQFDHCIVGEPSNVETIGD
HHHHCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHCCCCCCEECCCCCCCHHHHH
TIKIGRRGSQSGVLIVDGVQGHVAYPHRAANPVPDIARLITTLNDEPLDHGSAQFQPSNL
HHHHCCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLKALVEQRLAAACGNRIRAHIEWLP
EEEEEECCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
SNADVFVTKPGAFTDLVGAAIAEVTGRTPELNTGGGTSDARFIAKYCQVVEFGLVGQTMH
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QIDERTPVSDLDKLTAIYRGVLQRYFA
HHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSTPTALSIAQDLLRCPSVTPADAGALDVLETLLKGAGFTVHRVTFSEPGTADIDNLYA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
RIGNTSPHLCFAGHTDVVPPGDASAWTHGAFAGDVADGLLYGRGAVDMKGGIACAVAATL
HHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHH
DYLAANGGQPKGSISFLITGDEEDVAVNGTVKLLQWAAERGEQFDHCIVGEPSNVETIGD
HHHHCCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHCCCCCCEECCCCCCCHHHHH
TIKIGRRGSQSGVLIVDGVQGHVAYPHRAANPVPDIARLITTLNDEPLDHGSAQFQPSNL
HHHHCCCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFTSVDVGNPATNVIPAQARAKFNIRFNDHHTQETLKALVEQRLAAACGNRIRAHIEWLP
EEEEEECCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
SNADVFVTKPGAFTDLVGAAIAEVTGRTPELNTGGGTSDARFIAKYCQVVEFGLVGQTMH
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QIDERTPVSDLDKLTAIYRGVLQRYFA
HHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA