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The map label for this gene is pbpG [H]

Identifier: 192289010

GI number: 192289010

Start: 629453

End: 631672

Strand: Reverse

Name: pbpG [H]

Synonym: Rpal_0580

Alternate gene names: 192289010

Gene position: 631672-629453 (Counterclockwise)

Preceding gene: 192289012

Following gene: 192289005

Centisome position: 11.0

GC content: 68.2

Gene sequence:

>2220_bases
ATGGCGTGGGGCAGGAAAAAAGCCGGCGAGAGGCGAGAGCCGAAATTCGGGTCCGGTGAATCGCTCGCCGACATGCGCCT
GACGCCGCAGGATCGGGTGAACGTGGCCGACGACAAGCCGAAGAAGAAGCCCGCGGGCAAGGCCGGCGGCGGAGCGCGCA
GCAAGCGCAAGCCGCGCAAGGGCGTGATCGCGTCGGTGCGCCAGGCGGTCGGCCGCGTGGTGTATTGGAGCGCGGTCGCC
GGCATCTGGGCGGTGCTGGCCGTGGTCGGCGTCATGGTCTGGGTCGGCGCGCACCTGCCGCCGATCCAGTCGCTGGAGAT
TCCGAAGCGGCCGCCGACCATCACCATCGTGGCCGACGACGGCTCGACCATGGCGGTGCGCGGCGAGCAGGCCGGCACCA
ACGTGGCGCTCAAGGAGCTGCCGCCGTACCTACCGAAAGCATTCATCGCGATCGAAGACCGCCGGTTCTATCAGCACTTC
GGCGTCGATCCGCTCGGCATCGCCCGCGCCGCGGTGGCCAACGTGCTGCACCGGGGCGTGTCGCAGGGCGGTTCGACGCT
GACCCAGCAGCTCGCCAAGAACCTGTTCCTAACGCAGGACCGCACGCTGACCCGCAAGCTGCAGGAGGTCGAGCTGGCGC
TGTGGCTGGAGCGCAAGCACTCCAAGGCCGAGATCCTCGAACTGTATCTGAACCGGGTCTATTTCGGCTCCGGCGCCTAC
GGGGTCGAGGCCGCGGCGCAGAAGTATTTCGGCAAGTCGGCCAAGGACGTCACGCTGGTCGAGGCGGCGATGCTGGCCGG
TCTCGTCAAATCGCCTTCGCGCCTTGCGCCGAACCGCAATCCCGAAGGCGCCGAAAAGCGCGCCCAGATCGTGTTGGCTG
CCATGGCCGACGCTGGCTTCGTCACCGACAAGCAGGCCCAGGCCGCGATCGCCAACCCGGTCTACACCGTCAAGCCGACC
GGTGCCGGCACCATCAACTACGTCGCCGACTGGATCACCGAGATCCTGGATGATCTGGTCGGCCAGATCGACCAGTCGAT
CATCGTCGAGACCTCGATCGATCCGAGGCTGCAGTCGGTCGCCGAGGCCGCGATCATCGACGAGCTGGCGGCCAAGAGCG
TCAAATACAAGGTGACGCAGGGGGCGCTGGTGGCGATGACGCCGGACGGCACGGTGCGGGCCATGATCGGCGGGCGCAAT
TACGCCGACAGCCAGTTCAACCGCGCCGTCACCGCCAAGCGGCAGCCGGGCTCTGCGTTCAAGCCGTTCGTGTATCTGAC
GGCGGTCGAAGCTGGGCTGACACCCGACACCATCCGCCAGGATGCGCCGCTCGATCTCAAAGGCTGGAAGCCGGAGAACT
ACACCCACGAATATTTCGGCTCGGTGACGCTGACCCAGGCGCTGGCGATGTCGCTGAACACGGTGGCGGTGCGGCTCGGC
CTCGAGGTCGGGCCGAAGAACGTGGTGCGGACCGCGCACCGGCTCGGCATCGCCTCCAAGCTCGATGCCAACCCGTCGAT
CGCGCTCGGCACCTCTGAAGTCACGCTGACCGAGCTGGTCGGCGCCTACTCCACCTTCGCCAATGGCGGGCTATTGGTGT
CGCCGCATGTGGTGAAACGGATCCGCACCTTGCAGGGCAACAAGGTGCTGTACGTCCGCAGCGACGAGCCGGCGAGCCGG
GTGGTCGATCCGCGTGCGGTGGCGATGATGAACACCATGATGCAGGAGACGCTGCGCTCCGGCACCGCCCACAAGGCGGA
CCTGCCGGGCTGGGTCGCGGCCGGCAAAACCGGCACCAGCCAGGATTTCCGCGACGCCTGGTTCCTCGGCTACACCTCCA
CGCTGGTCACCGGCGTCTGGCTCGGCAACGACGACAACTCGCCGACCAAGAAGGCGACCGGCGGCGGATTGCCGGTCGAA
GTGTGGACGCGGTTCATGCGCGAAGCGCTCAAGGGCGAGACTCCGCAGCCCTTGCCGGGCGACTGGAGCGGCGGCCCGAT
CGTCAGCAGCACGCCCTCCGCGCTCGGCGGGAGCCTAAGCGGCGCCCCGACGGGCGGCGGCACCATCGCGCAAGCCGGAC
CGCGCGGCGAAACCCAGGTGATCAACGCTCCGCCGCCCCCAGGGTCAGGCCGGATGCCTCCGCCGACCCGCGCCAATGTG
CGACCGGAGGCCGACGCCGGCCTGGATGGATGGCTGATGGATCGGCTGTTCGGGCGGTGA

Upstream 100 bases:

>100_bases
GCGGTTCCGTCAATTCGGGAACCCGACTTTAACCTTCGCTTTACCGGGCCTTAAACCGGCTTCGCTAGCGTCGCCGGTAA
CGGTCGGCGTGGACGATGCG

Downstream 100 bases:

>100_bases
TGCGTCGTGCAGATCTGAGCGGTTAGTAGCGGGCGTCACCCGCTTGGCTGCCCTCACCCCACCCCTCTCTCGCGAGCGGG
AGAGGGATCGCGCCGCACGC

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MAWGRKKAGERREPKFGSGESLADMRLTPQDRVNVADDKPKKKPAGKAGGGARSKRKPRKGVIASVRQAVGRVVYWSAVA
GIWAVLAVVGVMVWVGAHLPPIQSLEIPKRPPTITIVADDGSTMAVRGEQAGTNVALKELPPYLPKAFIAIEDRRFYQHF
GVDPLGIARAAVANVLHRGVSQGGSTLTQQLAKNLFLTQDRTLTRKLQEVELALWLERKHSKAEILELYLNRVYFGSGAY
GVEAAAQKYFGKSAKDVTLVEAAMLAGLVKSPSRLAPNRNPEGAEKRAQIVLAAMADAGFVTDKQAQAAIANPVYTVKPT
GAGTINYVADWITEILDDLVGQIDQSIIVETSIDPRLQSVAEAAIIDELAAKSVKYKVTQGALVAMTPDGTVRAMIGGRN
YADSQFNRAVTAKRQPGSAFKPFVYLTAVEAGLTPDTIRQDAPLDLKGWKPENYTHEYFGSVTLTQALAMSLNTVAVRLG
LEVGPKNVVRTAHRLGIASKLDANPSIALGTSEVTLTELVGAYSTFANGGLLVSPHVVKRIRTLQGNKVLYVRSDEPASR
VVDPRAVAMMNTMMQETLRSGTAHKADLPGWVAAGKTGTSQDFRDAWFLGYTSTLVTGVWLGNDDNSPTKKATGGGLPVE
VWTRFMREALKGETPQPLPGDWSGGPIVSSTPSALGGSLSGAPTGGGTIAQAGPRGETQVINAPPPPGSGRMPPPTRANV
RPEADAGLDGWLMDRLFGR

Sequences:

>Translated_739_residues
MAWGRKKAGERREPKFGSGESLADMRLTPQDRVNVADDKPKKKPAGKAGGGARSKRKPRKGVIASVRQAVGRVVYWSAVA
GIWAVLAVVGVMVWVGAHLPPIQSLEIPKRPPTITIVADDGSTMAVRGEQAGTNVALKELPPYLPKAFIAIEDRRFYQHF
GVDPLGIARAAVANVLHRGVSQGGSTLTQQLAKNLFLTQDRTLTRKLQEVELALWLERKHSKAEILELYLNRVYFGSGAY
GVEAAAQKYFGKSAKDVTLVEAAMLAGLVKSPSRLAPNRNPEGAEKRAQIVLAAMADAGFVTDKQAQAAIANPVYTVKPT
GAGTINYVADWITEILDDLVGQIDQSIIVETSIDPRLQSVAEAAIIDELAAKSVKYKVTQGALVAMTPDGTVRAMIGGRN
YADSQFNRAVTAKRQPGSAFKPFVYLTAVEAGLTPDTIRQDAPLDLKGWKPENYTHEYFGSVTLTQALAMSLNTVAVRLG
LEVGPKNVVRTAHRLGIASKLDANPSIALGTSEVTLTELVGAYSTFANGGLLVSPHVVKRIRTLQGNKVLYVRSDEPASR
VVDPRAVAMMNTMMQETLRSGTAHKADLPGWVAAGKTGTSQDFRDAWFLGYTSTLVTGVWLGNDDNSPTKKATGGGLPVE
VWTRFMREALKGETPQPLPGDWSGGPIVSSTPSALGGSLSGAPTGGGTIAQAGPRGETQVINAPPPPGSGRMPPPTRANV
RPEADAGLDGWLMDRLFGR
>Mature_738_residues
AWGRKKAGERREPKFGSGESLADMRLTPQDRVNVADDKPKKKPAGKAGGGARSKRKPRKGVIASVRQAVGRVVYWSAVAG
IWAVLAVVGVMVWVGAHLPPIQSLEIPKRPPTITIVADDGSTMAVRGEQAGTNVALKELPPYLPKAFIAIEDRRFYQHFG
VDPLGIARAAVANVLHRGVSQGGSTLTQQLAKNLFLTQDRTLTRKLQEVELALWLERKHSKAEILELYLNRVYFGSGAYG
VEAAAQKYFGKSAKDVTLVEAAMLAGLVKSPSRLAPNRNPEGAEKRAQIVLAAMADAGFVTDKQAQAAIANPVYTVKPTG
AGTINYVADWITEILDDLVGQIDQSIIVETSIDPRLQSVAEAAIIDELAAKSVKYKVTQGALVAMTPDGTVRAMIGGRNY
ADSQFNRAVTAKRQPGSAFKPFVYLTAVEAGLTPDTIRQDAPLDLKGWKPENYTHEYFGSVTLTQALAMSLNTVAVRLGL
EVGPKNVVRTAHRLGIASKLDANPSIALGTSEVTLTELVGAYSTFANGGLLVSPHVVKRIRTLQGNKVLYVRSDEPASRV
VDPRAVAMMNTMMQETLRSGTAHKADLPGWVAAGKTGTSQDFRDAWFLGYTSTLVTGVWLGNDDNSPTKKATGGGLPVEV
WTRFMREALKGETPQPLPGDWSGGPIVSSTPSALGGSLSGAPTGGGTIAQAGPRGETQVINAPPPPGSGRMPPPTRANVR
PEADAGLDGWLMDRLFGR

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=602, Percent_Identity=32.2259136212625, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1788867, Length=571, Percent_Identity=30.6479859894921, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87082258, Length=314, Percent_Identity=35.9872611464968, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=39.3939393939394, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 78865; Mature: 78734

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWGRKKAGERREPKFGSGESLADMRLTPQDRVNVADDKPKKKPAGKAGGGARSKRKPRK
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
GVIASVRQAVGRVVYWSAVAGIWAVLAVVGVMVWVGAHLPPIQSLEIPKRPPTITIVADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
GSTMAVRGEQAGTNVALKELPPYLPKAFIAIEDRRFYQHFGVDPLGIARAAVANVLHRGV
CCEEEEECCCCCCCEEHHHCCCCCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SQGGSTLTQQLAKNLFLTQDRTLTRKLQEVELALWLERKHSKAEILELYLNRVYFGSGAY
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCC
GVEAAAQKYFGKSAKDVTLVEAAMLAGLVKSPSRLAPNRNPEGAEKRAQIVLAAMADAGF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHEEEEEHHCCCC
VTDKQAQAAIANPVYTVKPTGAGTINYVADWITEILDDLVGQIDQSIIVETSIDPRLQSV
CCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
AEAAIIDELAAKSVKYKVTQGALVAMTPDGTVRAMIGGRNYADSQFNRAVTAKRQPGSAF
HHHHHHHHHHHHHEEEEEECCEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCC
KPFVYLTAVEAGLTPDTIRQDAPLDLKGWKPENYTHEYFGSVTLTQALAMSLNTVAVRLG
CCEEEEEEHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHEEEEEC
LEVGPKNVVRTAHRLGIASKLDANPSIALGTSEVTLTELVGAYSTFANGGLLVSPHVVKR
CCCCHHHHHHHHHHHCCHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECHHHHHH
IRTLQGNKVLYVRSDEPASRVVDPRAVAMMNTMMQETLRSGTAHKADLPGWVAAGKTGTS
HHHCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCC
QDFRDAWFLGYTSTLVTGVWLGNDDNSPTKKATGGGLPVEVWTRFMREALKGETPQPLPG
CHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
DWSGGPIVSSTPSALGGSLSGAPTGGGTIAQAGPRGETQVINAPPPPGSGRMPPPTRANV
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RPEADAGLDGWLMDRLFGR
CCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AWGRKKAGERREPKFGSGESLADMRLTPQDRVNVADDKPKKKPAGKAGGGARSKRKPRK
CCCCCCCCCCCCCCCCCCCCCHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
GVIASVRQAVGRVVYWSAVAGIWAVLAVVGVMVWVGAHLPPIQSLEIPKRPPTITIVADD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
GSTMAVRGEQAGTNVALKELPPYLPKAFIAIEDRRFYQHFGVDPLGIARAAVANVLHRGV
CCEEEEECCCCCCCEEHHHCCCCCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SQGGSTLTQQLAKNLFLTQDRTLTRKLQEVELALWLERKHSKAEILELYLNRVYFGSGAY
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCC
GVEAAAQKYFGKSAKDVTLVEAAMLAGLVKSPSRLAPNRNPEGAEKRAQIVLAAMADAGF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHEEEEEHHCCCC
VTDKQAQAAIANPVYTVKPTGAGTINYVADWITEILDDLVGQIDQSIIVETSIDPRLQSV
CCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
AEAAIIDELAAKSVKYKVTQGALVAMTPDGTVRAMIGGRNYADSQFNRAVTAKRQPGSAF
HHHHHHHHHHHHHEEEEEECCEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCC
KPFVYLTAVEAGLTPDTIRQDAPLDLKGWKPENYTHEYFGSVTLTQALAMSLNTVAVRLG
CCEEEEEEHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHEEEEEC
LEVGPKNVVRTAHRLGIASKLDANPSIALGTSEVTLTELVGAYSTFANGGLLVSPHVVKR
CCCCHHHHHHHHHHHCCHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECHHHHHH
IRTLQGNKVLYVRSDEPASRVVDPRAVAMMNTMMQETLRSGTAHKADLPGWVAAGKTGTS
HHHCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCC
QDFRDAWFLGYTSTLVTGVWLGNDDNSPTKKATGGGLPVEVWTRFMREALKGETPQPLPG
CHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
DWSGGPIVSSTPSALGGSLSGAPTGGGTIAQAGPRGETQVINAPPPPGSGRMPPPTRANV
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RPEADAGLDGWLMDRLFGR
CCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]