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The map label for this gene is purC2 [H]

Identifier: 192288990

GI number: 192288990

Start: 608997

End: 609914

Strand: Reverse

Name: purC2 [H]

Synonym: Rpal_0560

Alternate gene names: 192288990

Gene position: 609914-608997 (Counterclockwise)

Preceding gene: 192288991

Following gene: 192288988

Centisome position: 10.62

GC content: 66.88

Gene sequence:

>918_bases
ATGACCACGATGCTCTCGAGCGACCTGCCGCTGACCAAGATCGGCCGCGGCAAGGTGCGCGACATCTATGCCGTCGACGA
CGACCGGCTGCTGCTCGTCACCACCGACCGGATCAGCGCCTTCGACGTGGTGATGGGCGAGACCATTCCGATGAAGGGCG
CCGTGCTGACCCAGGTGAGCGCATTCTGGTTCGGCCAGCTCGGCGGCGTGGTGCCGCATCACATGATCAGCGCCGACATC
GACGAGATCATCGCCGCGGTCCCGGCACTGCAGGGCCTTCGCGCAGCACTGGCCGGCCGCACCATGCTGTGCCGACGCAC
CACCGTATTCCCGATCGAATGCGTGATCCGTGGCTACATCACCGGCTCGGCATGGAAGGAATACGCCGCCTCCGGCACGC
TGGCGGGCGAGAAGCTGGCCGCGGGGCTGACGGAGAGCGAGCGGCTGGAGCCGCCGATCTTCTCGCCGGCCACCAAGGCC
GAGACCGGTCACGACGAGAACATCACCATCGCACGGATGCGTGAGATCGTCGGCGACGAGGCCGCCTACACGCTCGAAAG
CATGACGCGCGCGATCTACACCCAGGGCGAAGAGGTCGCCCGCGAGCAGGGCATCATCATCGCCGACACCAAGTTCGAGT
TCGGCCGCGACAAGGACGGCCGCATCATCCTGATCGACGAGGTGATGACGCCGGATAGTTCGCGGTTCTGGGCGGCCGAT
ACGTACGCGCCGGGCCGGTCGCAGCCGTCATTCGACAAGCAGCCGCTGCGCGATTATCTCGACGCCGAACGCCGTGCCGG
CCGCTGGAACGGCGAAGCGCCGCCGCCGAAGCTGCCGGCCGAGATCGTCGAGGCGACCTCGAAGCGCTATCTCGATGCCT
ACCGCCGGCTCACCGGCACCGATCTGGTGGTGACTTAA

Upstream 100 bases:

>100_bases
GAGAGCGAGTTCATTCCGCTGCCGGAATGATAGACTGAGGGCCCGCAACGATCCCCGCCGCCGGCCGTGCCGGCGGCCGC
CTTTGCCTGGAACACCGCCC

Downstream 100 bases:

>100_bases
GGTCACTCCGTGCTGAGCCGGATCGCGGGCATGGCTCCCGCAATCCGGCTGTAGCCTCACGGCCTGGTCGATCAGACGCC
TTCGAACAGCACCGTCGACA

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase 2 [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTTMLSSDLPLTKIGRGKVRDIYAVDDDRLLLVTTDRISAFDVVMGETIPMKGAVLTQVSAFWFGQLGGVVPHHMISADI
DEIIAAVPALQGLRAALAGRTMLCRRTTVFPIECVIRGYITGSAWKEYAASGTLAGEKLAAGLTESERLEPPIFSPATKA
ETGHDENITIARMREIVGDEAAYTLESMTRAIYTQGEEVAREQGIIIADTKFEFGRDKDGRIILIDEVMTPDSSRFWAAD
TYAPGRSQPSFDKQPLRDYLDAERRAGRWNGEAPPPKLPAEIVEATSKRYLDAYRRLTGTDLVVT

Sequences:

>Translated_305_residues
MTTMLSSDLPLTKIGRGKVRDIYAVDDDRLLLVTTDRISAFDVVMGETIPMKGAVLTQVSAFWFGQLGGVVPHHMISADI
DEIIAAVPALQGLRAALAGRTMLCRRTTVFPIECVIRGYITGSAWKEYAASGTLAGEKLAAGLTESERLEPPIFSPATKA
ETGHDENITIARMREIVGDEAAYTLESMTRAIYTQGEEVAREQGIIIADTKFEFGRDKDGRIILIDEVMTPDSSRFWAAD
TYAPGRSQPSFDKQPLRDYLDAERRAGRWNGEAPPPKLPAEIVEATSKRYLDAYRRLTGTDLVVT
>Mature_304_residues
TTMLSSDLPLTKIGRGKVRDIYAVDDDRLLLVTTDRISAFDVVMGETIPMKGAVLTQVSAFWFGQLGGVVPHHMISADID
EIIAAVPALQGLRAALAGRTMLCRRTTVFPIECVIRGYITGSAWKEYAASGTLAGEKLAAGLTESERLEPPIFSPATKAE
TGHDENITIARMREIVGDEAAYTLESMTRAIYTQGEEVAREQGIIIADTKFEFGRDKDGRIILIDEVMTPDSSRFWAADT
YAPGRSQPSFDKQPLRDYLDAERRAGRWNGEAPPPKLPAEIVEATSKRYLDAYRRLTGTDLVVT

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788820, Length=230, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319326, Length=304, Percent_Identity=47.0394736842105, Blast_Score=265, Evalue=9e-72,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 33545; Mature: 33413

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMLSSDLPLTKIGRGKVRDIYAVDDDRLLLVTTDRISAFDVVMGETIPMKGAVLTQVS
CCCCCCCCCCHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHH
AFWFGQLGGVVPHHMISADIDEIIAAVPALQGLRAALAGRTMLCRRTTVFPIECVIRGYI
HHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
TGSAWKEYAASGTLAGEKLAAGLTESERLEPPIFSPATKAETGHDENITIARMREIVGDE
CCHHHHHHHCCCCCCHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCH
AAYTLESMTRAIYTQGEEVAREQGIIIADTKFEFGRDKDGRIILIDEVMTPDSSRFWAAD
HHHHHHHHHHHHHHCCHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEC
TYAPGRSQPSFDKQPLRDYLDAERRAGRWNGEAPPPKLPAEIVEATSKRYLDAYRRLTGT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DLVVT
CEEEC
>Mature Secondary Structure 
TTMLSSDLPLTKIGRGKVRDIYAVDDDRLLLVTTDRISAFDVVMGETIPMKGAVLTQVS
CCCCCCCCCHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHH
AFWFGQLGGVVPHHMISADIDEIIAAVPALQGLRAALAGRTMLCRRTTVFPIECVIRGYI
HHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
TGSAWKEYAASGTLAGEKLAAGLTESERLEPPIFSPATKAETGHDENITIARMREIVGDE
CCHHHHHHHCCCCCCHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCH
AAYTLESMTRAIYTQGEEVAREQGIIIADTKFEFGRDKDGRIILIDEVMTPDSSRFWAAD
HHHHHHHHHHHHHHCCHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEC
TYAPGRSQPSFDKQPLRDYLDAERRAGRWNGEAPPPKLPAEIVEATSKRYLDAYRRLTGT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DLVVT
CEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]