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The map label for this gene is braC [H]

Identifier: 190894960

GI number: 190894960

Start: 678229

End: 679422

Strand: Reverse

Name: braC [H]

Synonym: RHECIAT_PC0000625

Alternate gene names: 190894960

Gene position: 679422-678229 (Counterclockwise)

Preceding gene: 190894963

Following gene: 190894959

Centisome position: 62.25

GC content: 60.05

Gene sequence:

>1194_bases
ATGAACAAGACTTTGACGCGCCGGGCATTTTGCATTTCCACGGCAATCCTTTCCTTGACCGCGCTCATGACCGGCGTCTC
GCAACGGGCTCTCGCCGCAGATGAAACGATCAGGCTCGGCCTGGTATCGCCGATGAGCGGGCCGAATGCCCGTTACGGCG
CGTTTTCGCTGCATGGCGCCGAACTGGCCGTCAAAGAAATCAACGATGCCGGCGGTATTGACGGCCGCAAGATCGAAATC
TTTTCCGCCGACAGCCAGGGAACGCCGGTCGAGGGCGTTTCGGCCACGCGGCGCCTGATCGACCAGGACAAGGTCAACTA
CATCATCGGTGACGTTTCGAGCTCGGTCACTCTGGCGATGCAGCCGGTCGCCGAAGATGCGGGCGTGCTTCTGCTGAACG
CCGCATCGTCGAACCCGAAGATCACCTATGGCGCCGGCGTCGGCGGTTACAAGTGGACCTTCCGCAACTATCCCACAGAC
GAGAACCGGGCGCTGATTGTGGCGAAATATGCCGCCGAGCAGCGCGGCTTCACCAAATTCGCCGTTCTCTCGGTCGACAG
CGATTACGGCCGTTCGGCGATCACCTTCACCAAGAAATACCTGCCCGACTTCAAGGGCGAGATCGTCAGCGAAGACTATT
ACAAGGAAGGCGAGGTCGACTTCCGCAGCGTTCTCGCCAAGATCCGTGACAATGGCGCCCAGGCGATCATCATGTACGGC
CTTGCCGATACCACGCCAATTATCGCCCGTCAGATGCTCGAACTCGGGCTGGCCGGCAAGGTGACCCTGATTGGCAATGG
CGAGTTCAACACCGAAAAGACCATCAAATCAGCGCCGAAGGTCCTTGAAGGCGCGGTCGAAGCCGCGGCATGGCTGCCGG
CCTTCGACTCCCCCGAGAGCAAGGCTTTCGTGGAGAAGTTCACGGCTGCCTACAATGAGGCGCCGAACAACCACGCCTAT
GTCCATTGGGACACGGTTCACCTTCTCGCCCAGGCGATCAAGGAAGCCGGCAGCCCCGACCAGGCCAAGGTGCGTGATGC
GCTCTCCAAGATCAAATATAAGAGCGCCGTCGGAGAGGTGACCTTCGACGATCACAACCAGGCTCGCCTGCCGATGATCC
TGCTGCAGATCGAGGACGGCAAGCCGAGCATCAAAGGTGCCTACGCGGCGGATATCCAGTATCCTGCGAACTGA

Upstream 100 bases:

>100_bases
GTCAGCAGAAGACTGGCGCAACAAGAACCGCGGCAAAGCGGCATTTGAGCACATGACGGCAGGGGCCGATCAGACGTGTC
CAACAAACGGAGTGGGAACC

Downstream 100 bases:

>100_bases
TCCACCGTCGGGGATAGGACCATGTTCTCAACAATCCTTGAGCAGATCATCAACGGCATCGTGACGGGATCCGTCTACGC
CATCGTTGCCGTTGGCATGA

Product: putative amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKTLTRRAFCISTAILSLTALMTGVSQRALAADETIRLGLVSPMSGPNARYGAFSLHGAELAVKEINDAGGIDGRKIEI
FSADSQGTPVEGVSATRRLIDQDKVNYIIGDVSSSVTLAMQPVAEDAGVLLLNAASSNPKITYGAGVGGYKWTFRNYPTD
ENRALIVAKYAAEQRGFTKFAVLSVDSDYGRSAITFTKKYLPDFKGEIVSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
LADTTPIIARQMLELGLAGKVTLIGNGEFNTEKTIKSAPKVLEGAVEAAAWLPAFDSPESKAFVEKFTAAYNEAPNNHAY
VHWDTVHLLAQAIKEAGSPDQAKVRDALSKIKYKSAVGEVTFDDHNQARLPMILLQIEDGKPSIKGAYAADIQYPAN

Sequences:

>Translated_397_residues
MNKTLTRRAFCISTAILSLTALMTGVSQRALAADETIRLGLVSPMSGPNARYGAFSLHGAELAVKEINDAGGIDGRKIEI
FSADSQGTPVEGVSATRRLIDQDKVNYIIGDVSSSVTLAMQPVAEDAGVLLLNAASSNPKITYGAGVGGYKWTFRNYPTD
ENRALIVAKYAAEQRGFTKFAVLSVDSDYGRSAITFTKKYLPDFKGEIVSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
LADTTPIIARQMLELGLAGKVTLIGNGEFNTEKTIKSAPKVLEGAVEAAAWLPAFDSPESKAFVEKFTAAYNEAPNNHAY
VHWDTVHLLAQAIKEAGSPDQAKVRDALSKIKYKSAVGEVTFDDHNQARLPMILLQIEDGKPSIKGAYAADIQYPAN
>Mature_397_residues
MNKTLTRRAFCISTAILSLTALMTGVSQRALAADETIRLGLVSPMSGPNARYGAFSLHGAELAVKEINDAGGIDGRKIEI
FSADSQGTPVEGVSATRRLIDQDKVNYIIGDVSSSVTLAMQPVAEDAGVLLLNAASSNPKITYGAGVGGYKWTFRNYPTD
ENRALIVAKYAAEQRGFTKFAVLSVDSDYGRSAITFTKKYLPDFKGEIVSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
LADTTPIIARQMLELGLAGKVTLIGNGEFNTEKTIKSAPKVLEGAVEAAAWLPAFDSPESKAFVEKFTAAYNEAPNNHAY
VHWDTVHLLAQAIKEAGSPDQAKVRDALSKIKYKSAVGEVTFDDHNQARLPMILLQIEDGKPSIKGAYAADIQYPAN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=362, Percent_Identity=24.0331491712707, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994941, Length=242, Percent_Identity=29.7520661157025, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42858; Mature: 42858

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTLTRRAFCISTAILSLTALMTGVSQRALAADETIRLGLVSPMSGPNARYGAFSLHGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCC
ELAVKEINDAGGIDGRKIEIFSADSQGTPVEGVSATRRLIDQDKVNYIIGDVSSSVTLAM
CEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEE
QPVAEDAGVLLLNAASSNPKITYGAGVGGYKWTFRNYPTDENRALIVAKYAAEQRGFTKF
ECCCCCCCEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCCEEEEEEECHHHCCCEEE
AVLSVDSDYGRSAITFTKKYLPDFKGEIVSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
EEEEECCCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
LADTTPIIARQMLELGLAGKVTLIGNGEFNTEKTIKSAPKVLEGAVEAAAWLPAFDSPES
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
KAFVEKFTAAYNEAPNNHAYVHWDTVHLLAQAIKEAGSPDQAKVRDALSKIKYKSAVGEV
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCE
TFDDHNQARLPMILLQIEDGKPSIKGAYAADIQYPAN
EECCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MNKTLTRRAFCISTAILSLTALMTGVSQRALAADETIRLGLVSPMSGPNARYGAFSLHGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCC
ELAVKEINDAGGIDGRKIEIFSADSQGTPVEGVSATRRLIDQDKVNYIIGDVSSSVTLAM
CEEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEE
QPVAEDAGVLLLNAASSNPKITYGAGVGGYKWTFRNYPTDENRALIVAKYAAEQRGFTKF
ECCCCCCCEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCCEEEEEEECHHHCCCEEE
AVLSVDSDYGRSAITFTKKYLPDFKGEIVSEDYYKEGEVDFRSVLAKIRDNGAQAIIMYG
EEEEECCCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
LADTTPIIARQMLELGLAGKVTLIGNGEFNTEKTIKSAPKVLEGAVEAAAWLPAFDSPES
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
KAFVEKFTAAYNEAPNNHAYVHWDTVHLLAQAIKEAGSPDQAKVRDALSKIKYKSAVGEV
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCE
TFDDHNQARLPMILLQIEDGKPSIKGAYAADIQYPAN
EECCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]