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The map label for this gene is nac [H]

Identifier: 190894954

GI number: 190894954

Start: 672558

End: 673496

Strand: Reverse

Name: nac [H]

Synonym: RHECIAT_PC0000619

Alternate gene names: 190894954

Gene position: 673496-672558 (Counterclockwise)

Preceding gene: 190894955

Following gene: 190894953

Centisome position: 61.7

GC content: 63.15

Gene sequence:

>939_bases
ATGAGCGTAGATTTCAGAAAACTGCGAAGTTTCGTCAAGATCGTCGATACCGGCAGCGTTTCGCGCGCAGCCGCCCTGCT
TCGCACCGCTCAGCCTGCGCTCTCCCAGCAGATCGCCGCGCTGGAGGCCCATTTCAAGCACAAGCTTCTGATCCGAAGCA
ATGTCGGCATCACCCCGACCGAGGCCGGCCTGATCCTCTATCGGCATGCCCAGCTGATGCTGAAGCAGATCGATCAGGCG
CAGACCGATATCAACCAGTCCGCAAAGTCGGTGGCCGGACGGGTATCGATCGGGCTTGCGACCTATTCGACGTCGAGCGC
CTTGTCGCTGCCGATCCTGAAGGAAATGAAAGCCCGTCATCCCGACGTCGTCGTCCACATAAACGACAGCTTCGGCCATA
TCCTCAGCGAACTCATCATGACCGGCAAGATGGACATGGCGCTGATCTATGCCGCAGACCCCATCAAAGGGGTGACGCTC
CAGCCGCTGTTCCGGGAGCAGATGTTCCTGGTATCACCGCCGGGGGCGGAATTGCCCGGCGACCCGTCCGAGCCTCTGCC
GCTTGCATCGGTCGATGCCCTGCCGCTGCTGCTGCCCAGCAAAGGCCATCTGCTTCGCCGGCTCATCGACGAAGCGTTCG
CCCGCGCCCGCGCCCATCCGCAAGTGCTGTCGGAGATCGAGTCGGTGCCGGCGCTCGACGCCGCGGTACGGGAAGGGCTG
GGCTCCACCATCCTTCCGGCATCGGTGGTGACCGAAACCTCCTATTTCGCCGGCACGCAGGTGCGGGCGCTGACCAAGCC
CGTCATCGAAGCCACCGTCTCCCTCTGCGTCTCCGACCATCTGCCTCTGTCCGAGCCGGCGCTCGCGGCGCGGGCCGTCC
TGCTGGAGATCGTTGCGAAGCTGATGAGCAGCCAGCATCAGGGTATCAAGACGGTTTAG

Upstream 100 bases:

>100_bases
GCGGGCGGTCGGCGAATTGCGTTGAATTCTGCAGCAGGCGGGAGGGGCTTGCGCGTTTCCCGCCCGAGGTCTTAGGTTCC
GTAAACCGAAGCTTGAGGCC

Downstream 100 bases:

>100_bases
AGCCGACCCGTTTTTCAACCGCCTGCCATAAGCAAACCCTATGGCGGCAGACGGCAACTGTGTTAGCCAAGCCGGTCGCC
GATCACCTAACCTCCTCAGC

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSVDFRKLRSFVKIVDTGSVSRAAALLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPTEAGLILYRHAQLMLKQIDQA
QTDINQSAKSVAGRVSIGLATYSTSSALSLPILKEMKARHPDVVVHINDSFGHILSELIMTGKMDMALIYAADPIKGVTL
QPLFREQMFLVSPPGAELPGDPSEPLPLASVDALPLLLPSKGHLLRRLIDEAFARARAHPQVLSEIESVPALDAAVREGL
GSTILPASVVTETSYFAGTQVRALTKPVIEATVSLCVSDHLPLSEPALAARAVLLEIVAKLMSSQHQGIKTV

Sequences:

>Translated_312_residues
MSVDFRKLRSFVKIVDTGSVSRAAALLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPTEAGLILYRHAQLMLKQIDQA
QTDINQSAKSVAGRVSIGLATYSTSSALSLPILKEMKARHPDVVVHINDSFGHILSELIMTGKMDMALIYAADPIKGVTL
QPLFREQMFLVSPPGAELPGDPSEPLPLASVDALPLLLPSKGHLLRRLIDEAFARARAHPQVLSEIESVPALDAAVREGL
GSTILPASVVTETSYFAGTQVRALTKPVIEATVSLCVSDHLPLSEPALAARAVLLEIVAKLMSSQHQGIKTV
>Mature_311_residues
SVDFRKLRSFVKIVDTGSVSRAAALLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPTEAGLILYRHAQLMLKQIDQAQ
TDINQSAKSVAGRVSIGLATYSTSSALSLPILKEMKARHPDVVVHINDSFGHILSELIMTGKMDMALIYAADPIKGVTLQ
PLFREQMFLVSPPGAELPGDPSEPLPLASVDALPLLLPSKGHLLRRLIDEAFARARAHPQVLSEIESVPALDAAVREGLG
STILPASVVTETSYFAGTQVRALTKPVIEATVSLCVSDHLPLSEPALAARAVLLEIVAKLMSSQHQGIKTV

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=297, Percent_Identity=36.026936026936, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI157672245, Length=272, Percent_Identity=27.9411764705882, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787601, Length=179, Percent_Identity=31.2849162011173, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=25.1012145748988, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=26.4822134387352, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790399, Length=285, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787806, Length=252, Percent_Identity=24.2063492063492, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787530, Length=255, Percent_Identity=25.8823529411765, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789204, Length=286, Percent_Identity=26.5734265734266, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33485; Mature: 33353

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDFRKLRSFVKIVDTGSVSRAAALLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
EAGLILYRHAQLMLKQIDQAQTDINQSAKSVAGRVSIGLATYSTSSALSLPILKEMKARH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHCCC
PDVVVHINDSFGHILSELIMTGKMDMALIYAADPIKGVTLQPLFREQMFLVSPPGAELPG
CCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHCCEEEECCCCCCCCC
DPSEPLPLASVDALPLLLPSKGHLLRRLIDEAFARARAHPQVLSEIESVPALDAAVREGL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCC
GSTILPASVVTETSYFAGTQVRALTKPVIEATVSLCVSDHLPLSEPALAARAVLLEIVAK
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LMSSQHQGIKTV
HHHHHCCCCCCC
>Mature Secondary Structure 
SVDFRKLRSFVKIVDTGSVSRAAALLRTAQPALSQQIAALEAHFKHKLLIRSNVGITPT
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
EAGLILYRHAQLMLKQIDQAQTDINQSAKSVAGRVSIGLATYSTSSALSLPILKEMKARH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHCCC
PDVVVHINDSFGHILSELIMTGKMDMALIYAADPIKGVTLQPLFREQMFLVSPPGAELPG
CCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHCCEEEECCCCCCCCC
DPSEPLPLASVDALPLLLPSKGHLLRRLIDEAFARARAHPQVLSEIESVPALDAAVREGL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCC
GSTILPASVVTETSYFAGTQVRALTKPVIEATVSLCVSDHLPLSEPALAARAVLLEIVAK
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LMSSQHQGIKTV
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]