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The map label for this gene is nrdA [H]

Identifier: 190571531

GI number: 190571531

Start: 1232709

End: 1234493

Strand: Reverse

Name: nrdA [H]

Synonym: WPa_1151

Alternate gene names: 190571531

Gene position: 1234493-1232709 (Counterclockwise)

Preceding gene: 190571532

Following gene: 190571530

Centisome position: 83.27

GC content: 35.01

Gene sequence:

>1785_bases
ATGAATTACATTGCTATTAACTACGCAAAGGATAGTAAATTAACTGATTTTGGAAAAGCAGTTCTATTGGACAGGTATTT
AATAGAAAATGAAAGTTATCAAGATCTCTTTATACGTATTGCCAATTACTACTCTGATAATAAAGAGCATGCACAGCGTC
TTTATGATTACATGAGCAATTTATGGTTCATGCCTTCAACTCCAATACTTAGTAATGGTGGTACCAAGAGAGGATTGCCT
ATTTCCTGCTTTCTTAACGAAACTGAAGATAGCTTGCAGGGAATAGTTGATTTATGGAATGAAAATGTTTGGCTCGCTGC
ACGTGGTGGTGGAATAGGTAGTTATTGGGGAAATTTACGTTCAATTGGTGAAAGTGTCAAAGGTAGCGGTAAAACATCAG
GAATTGTGCCATTTATCGTAGTACAAAATGCTCTGACGCTTGCAATCAGCCAAGGATCCTTAAGAAGAGGAAGCTCAGCA
GTTTATCTTCCTGTATGGCATCCAGAAATAGAAGAGTTTCTAGATTTACGTAAGCCAACTGGTGGTGACCCAAATCGCAA
AGCATTAAATATACATCATGCTGTAATAGTAACAGATAAGTTTATGCAAGCTGTTGAAGATGATCAAGAATGGGATTTAA
TAAGCCCTCATAACAATAAAGTTATTTCAACAGTAAAAGCACGGGATATATGGATTAAAATATTAACAGCAAGAGTTGAA
ACTGGAGAACCTTACATTATTTTCCTTGATGCAACAAATAACAATAAGCCAGAATCTTACAAAAAACTCAACTTAGACAT
CAAAATGTCAAACCTATGCAGTGAAATAACTTTAACTACAGGTTACGATCACCTGAAAAAGTCACGCACTGCTGTATGTT
GTTTATCATCCGTAAATCTTGAATACTACGAAGAATGGAAAGATAATGAGCTTTTCATAGAAGATATAATGCGTTTTCTT
GATAATGTATTAGAGGATTTCATAAACCAAGCACCAAATGAAATACAGCGAGCAAAATATTCCGCAATAAGAGAGCGTAG
TATCGGTCTTGGCGTGATGGGTTTTCATTCATTTTTACAAAGTAAAATGGTTCCTTTTGAATCAGTAACAGCCCAACAAT
GGAATAAAAAAGTATTTAAGCATTTGCGCGAGCAAGCAGATGTAGTTTCTAAAAAATTAGCAGAAGAAAAAGGTCCATGT
CTTGATGCTAAAGAAATCGATCTAATGGAAAGGTTTACACATAAGCTCGCTATTGCTCCAACTGCCTCAATTTCGATTAT
CGCAGGCAACACTTCCCCTGGAATAGAGCCATATGCAGCAAACGTATTTATACAGAAGACACTTACAGGTTCATTTGTAG
TACGAAATAAATTCTTGCAAAAACTACTAGCAGAAAAAAATCAAGACAATGATGAAATATGGTCCTCAATTTCAACAAAT
GAGGGTTCTGTTCAGCATTTAGATTTTCTTAGCAAGCATGAAAAACTGACATTTAAAACAGCATACGAACTTGATCAACA
ATGGATCATAGAACATGCAAGTGATAGAACTCCTTATATTTGTCAATCTCAATCAGTAAATCTGTTCTTACCTGCCAATG
TGCATAAACGTTACTTACACAAAATACATATGCTTGCTTGGAAAAAAGGATTAAAAAGCTTGTATTATTGCAGATCACAA
TCAATGCAGAGAGCTGACAAGGTCTCACATGACATATTCAAAAAAAGTGAAATATTGCAACAAAAAACAGATATTAATTA
TGATGAATGTCTATCATGCCAGTAG

Upstream 100 bases:

>100_bases
TGATGGTATCTTGTTTGCATTACAGTATCCACAAAAAAATTTAACAACTTAATCGAGAAATCGCTCAAATAAATGTCTAT
ATTATCATAAAGGGAGTCTT

Downstream 100 bases:

>100_bases
ACTGTACATAACTTACTGTCACAAAAATACAATTGTACAAAAGTTATGTTTTCATAGATGCTACATAGAAATCCAGATGA
ATTTCTAAAGTCTATTCCAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MNYIAINYAKDSKLTDFGKAVLLDRYLIENESYQDLFIRIANYYSDNKEHAQRLYDYMSNLWFMPSTPILSNGGTKRGLP
ISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLRSIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSA
VYLPVWHPEIEEFLDLRKPTGGDPNRKALNIHHAVIVTDKFMQAVEDDQEWDLISPHNNKVISTVKARDIWIKILTARVE
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLKKSRTAVCCLSSVNLEYYEEWKDNELFIEDIMRFL
DNVLEDFINQAPNEIQRAKYSAIRERSIGLGVMGFHSFLQSKMVPFESVTAQQWNKKVFKHLREQADVVSKKLAEEKGPC
LDAKEIDLMERFTHKLAIAPTASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDEIWSSISTN
EGSVQHLDFLSKHEKLTFKTAYELDQQWIIEHASDRTPYICQSQSVNLFLPANVHKRYLHKIHMLAWKKGLKSLYYCRSQ
SMQRADKVSHDIFKKSEILQQKTDINYDECLSCQ

Sequences:

>Translated_594_residues
MNYIAINYAKDSKLTDFGKAVLLDRYLIENESYQDLFIRIANYYSDNKEHAQRLYDYMSNLWFMPSTPILSNGGTKRGLP
ISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLRSIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSA
VYLPVWHPEIEEFLDLRKPTGGDPNRKALNIHHAVIVTDKFMQAVEDDQEWDLISPHNNKVISTVKARDIWIKILTARVE
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLKKSRTAVCCLSSVNLEYYEEWKDNELFIEDIMRFL
DNVLEDFINQAPNEIQRAKYSAIRERSIGLGVMGFHSFLQSKMVPFESVTAQQWNKKVFKHLREQADVVSKKLAEEKGPC
LDAKEIDLMERFTHKLAIAPTASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDEIWSSISTN
EGSVQHLDFLSKHEKLTFKTAYELDQQWIIEHASDRTPYICQSQSVNLFLPANVHKRYLHKIHMLAWKKGLKSLYYCRSQ
SMQRADKVSHDIFKKSEILQQKTDINYDECLSCQ
>Mature_594_residues
MNYIAINYAKDSKLTDFGKAVLLDRYLIENESYQDLFIRIANYYSDNKEHAQRLYDYMSNLWFMPSTPILSNGGTKRGLP
ISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLRSIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSA
VYLPVWHPEIEEFLDLRKPTGGDPNRKALNIHHAVIVTDKFMQAVEDDQEWDLISPHNNKVISTVKARDIWIKILTARVE
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLKKSRTAVCCLSSVNLEYYEEWKDNELFIEDIMRFL
DNVLEDFINQAPNEIQRAKYSAIRERSIGLGVMGFHSFLQSKMVPFESVTAQQWNKKVFKHLREQADVVSKKLAEEKGPC
LDAKEIDLMERFTHKLAIAPTASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDEIWSSISTN
EGSVQHLDFLSKHEKLTFKTAYELDQQWIIEHASDRTPYICQSQSVNLFLPANVHKRYLHKIHMLAWKKGLKSLYYCRSQ
SMQRADKVSHDIFKKSEILQQKTDINYDECLSCQ

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 ATP-cone domains [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=625, Percent_Identity=30.4, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1789030, Length=598, Percent_Identity=26.4214046822742, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI2367133, Length=634, Percent_Identity=24.2902208201893, Blast_Score=118, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17555242, Length=659, Percent_Identity=31.1077389984825, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6320914, Length=635, Percent_Identity=31.1811023622047, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322123, Length=636, Percent_Identity=30.188679245283, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI17352481, Length=625, Percent_Identity=29.44, Blast_Score=242, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 67886; Mature: 67886

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIAINYAKDSKLTDFGKAVLLDRYLIENESYQDLFIRIANYYSDNKEHAQRLYDYMSN
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
LWFMPSTPILSNGGTKRGLPISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLR
CEECCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
SIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSAVYLPVWHPEIEEFLDLRKPT
HHHHHHCCCCCCCCCCHHEEHHCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHCCCC
GGDPNRKALNIHHAVIVTDKFMQAVEDDQEWDLISPHNNKVISTVKARDIWIKILTARVE
CCCCCCCEEEEEEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHEEEEEEEEEC
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLKKSRTAVCCLSSVNL
CCCCEEEEEECCCCCCCCCHHEEEEEEEHHHHHHHHEEECCHHHHHHCCHHEEEEECCCH
EYYEEWKDNELFIEDIMRFLDNVLEDFINQAPNEIQRAKYSAIRERSIGLGVMGFHSFLQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKMVPFESVTAQQWNKKVFKHLREQADVVSKKLAEEKGPCLDAKEIDLMERFTHKLAIAP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECC
TASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDEIWSSISTN
CCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
EGSVQHLDFLSKHEKLTFKTAYELDQQWIIEHASDRTPYICQSQSVNLFLPANVHKRYLH
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHHHHH
KIHMLAWKKGLKSLYYCRSQSMQRADKVSHDIFKKSEILQQKTDINYDECLSCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MNYIAINYAKDSKLTDFGKAVLLDRYLIENESYQDLFIRIANYYSDNKEHAQRLYDYMSN
CCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
LWFMPSTPILSNGGTKRGLPISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLR
CEECCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
SIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSAVYLPVWHPEIEEFLDLRKPT
HHHHHHCCCCCCCCCCHHEEHHCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHCCCC
GGDPNRKALNIHHAVIVTDKFMQAVEDDQEWDLISPHNNKVISTVKARDIWIKILTARVE
CCCCCCCEEEEEEEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHEEEEEEEEEC
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLKKSRTAVCCLSSVNL
CCCCEEEEEECCCCCCCCCHHEEEEEEEHHHHHHHHEEECCHHHHHHCCHHEEEEECCCH
EYYEEWKDNELFIEDIMRFLDNVLEDFINQAPNEIQRAKYSAIRERSIGLGVMGFHSFLQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKMVPFESVTAQQWNKKVFKHLREQADVVSKKLAEEKGPCLDAKEIDLMERFTHKLAIAP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECC
TASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDEIWSSISTN
CCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
EGSVQHLDFLSKHEKLTFKTAYELDQQWIIEHASDRTPYICQSQSVNLFLPANVHKRYLH
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHHHHH
KIHMLAWKKGLKSLYYCRSQSMQRADKVSHDIFKKSEILQQKTDINYDECLSCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]