Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 190571527

GI number: 190571527

Start: 1229140

End: 1229637

Strand: Direct

Name: ruvC

Synonym: WPa_1146

Alternate gene names: 190571527

Gene position: 1229140-1229637 (Clockwise)

Preceding gene: 190571526

Following gene: 190571529

Centisome position: 82.91

GC content: 32.13

Gene sequence:

>498_bases
GTGATTAAAATAATAGGCCTAGATCCAGGGATGAGTAAGACTGGTTGGGCCATTATTAGACTGGAAGAAAAGAATAATAT
TAAATTTTTGGGTGGCGGTACCATATCAACTGATGGTAAATTGGGTGTAGGTGAACGTCTACATATAATTTTTGAACAAT
TAAAGAAAGTAATTTTCCTATACTCTCCAAATGAAGCTGCTGTGGAAAAAATTTTTATTAATAAGAATCCAAAATCTTCG
CTAACTTTAGGATATGCAAGAGCAATTGCAATTTTAATATTACGAATGACGGATTTACCCATGAATGAATATGATGCAAA
CTATATAAAAAAGAGCATTACTGGAAATGGTCATGCTGATAAGGGTCAGATTATATTTATGGTCAAGCAAATAGTTAAAA
GCTCAAATATAAAATGTCACCATACTGCTGATGCTCTTGCTACAGCAATTTGTCATGCTTATACGAAAAATTTTTGTTTT
ATGGGAATTGGTAGTTAA

Upstream 100 bases:

>100_bases
AGATCATTTATGTTTTGAGTTTGCTTCTTGGTATTGGCACTTTGTCGATCTAGTATGGATATTTTTATTTCTGTTTATTT
ATTGGTTAAGCCTTTATTAA

Downstream 100 bases:

>100_bases
TTCAAATTTTAAACTTGTAAGGCGTGGATTTTTGAAATCTTGCATCTAATTATAAATACATATCAGTTATCAGATTTAGA
ATATAGCAGAAATTGCTGCA

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIKIIGLDPGMSKTGWAIIRLEEKNNIKFLGGGTISTDGKLGVGERLHIIFEQLKKVIFLYSPNEAAVEKIFINKNPKSS
LTLGYARAIAILILRMTDLPMNEYDANYIKKSITGNGHADKGQIIFMVKQIVKSSNIKCHHTADALATAICHAYTKNFCF
MGIGS

Sequences:

>Translated_165_residues
MIKIIGLDPGMSKTGWAIIRLEEKNNIKFLGGGTISTDGKLGVGERLHIIFEQLKKVIFLYSPNEAAVEKIFINKNPKSS
LTLGYARAIAILILRMTDLPMNEYDANYIKKSITGNGHADKGQIIFMVKQIVKSSNIKCHHTADALATAICHAYTKNFCF
MGIGS
>Mature_165_residues
MIKIIGLDPGMSKTGWAIIRLEEKNNIKFLGGGTISTDGKLGVGERLHIIFEQLKKVIFLYSPNEAAVEKIFINKNPKSS
LTLGYARAIAILILRMTDLPMNEYDANYIKKSITGNGHADKGQIIFMVKQIVKSSNIKCHHTADALATAICHAYTKNFCF
MGIGS

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=157, Percent_Identity=35.031847133758, Blast_Score=85, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_WOLPP (B3CN07)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975885.1
- EnsemblBacteria:   EBWOLT00000000598
- GeneID:   6385150
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_1146
- GeneTree:   EBGT00050000031077
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   CLSK749295
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18188; Mature: 18188

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIIGLDPGMSKTGWAIIRLEEKNNIKFLGGGTISTDGKLGVGERLHIIFEQLKKVIFL
CEEEEECCCCCCCCCEEEEEEECCCCEEEEECCEECCCCCCCCCHHHHHHHHHHHHEEEE
YSPNEAAVEKIFINKNPKSSLTLGYARAIAILILRMTDLPMNEYDANYIKKSITGNGHAD
ECCCHHHEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCC
KGQIIFMVKQIVKSSNIKCHHTADALATAICHAYTKNFCFMGIGS
CCCEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEEEEECC
>Mature Secondary Structure
MIKIIGLDPGMSKTGWAIIRLEEKNNIKFLGGGTISTDGKLGVGERLHIIFEQLKKVIFL
CEEEEECCCCCCCCCEEEEEEECCCCEEEEECCEECCCCCCCCCHHHHHHHHHHHHEEEE
YSPNEAAVEKIFINKNPKSSLTLGYARAIAILILRMTDLPMNEYDANYIKKSITGNGHAD
ECCCHHHEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCC
KGQIIFMVKQIVKSSNIKCHHTADALATAICHAYTKNFCFMGIGS
CCCEEHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA