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The map label for this gene is metK [H]

Identifier: 190571523

GI number: 190571523

Start: 1225624

End: 1226790

Strand: Direct

Name: metK [H]

Synonym: WPa_1142

Alternate gene names: 190571523

Gene position: 1225624-1226790 (Clockwise)

Preceding gene: 190571522

Following gene: 190571524

Centisome position: 82.68

GC content: 37.02

Gene sequence:

>1167_bases
ATGGTTTTACATAAGAATTCAGTAACCAGTGAATCAGTAGCAGCCGGTCATCCAGACAAAGTAGCAGACCAGATTTCAGA
TGCAATACTTGACGAATACCTTTTTAATGACTCTTCCTCTCGTACTGCAATAGAGACTTTAGTTACTAAAGATAATGTTA
TTATAGCTGGGGAAGTATTTGGGCCGAACATTGAAAATAGCAAGATTGAAAGTATTGTACGGAATACAATAAAAGATATT
GGTTATGAGCATGATGGTTTCCACTGGGAAACAGTGAAAGTAAATATATTACTACATGAACAATCAAATGACATTGCAAT
AGGTGTGGATCAAGGTGCTGGGGATCAGGGCATAATGTATGGCTATGCAACAATAGAGACAGAAAGCCTCATGCCAGCAC
CCATTTTTTATGCACACTCGATTCTGAGAAATATCATGAGTATGGTCAAAGAGGTAAAGCTTGGTCCGGATGCAAAATCG
CAAATCACTTTGGCGTATGAGAATAACCTTCCAGTGCGTGCTGAAAGTATTGTTGTTTCAATACAGCATCTTGAGGATCT
GAGTCAATCGAAAGTGAAGGAAATAATTTATCCTTACATAGCTTCATCTTTACCTAAAGGGTGGATGTGCCCTAAAGAAA
ATCTCTTAGTCAATCCAACAGGTAGATTTGTTATTGGTGGACCAGTTGGTGATTGTGGATTAACCGGACGAAAAATTATG
GTTGATACTTATGGAGGCTATATTCCACACGGTGGAGGAGCGTTTTCTGGAAAAGATGCAACAAAAGTTGATAGATCCGC
AGCTTATATGGCGAGATACCTTGCTAAAAATATTGTCTTTGCAGGTTTAGCTGAACGTTGTCTTGTGCAGCTTTCTTATG
CAATTGGTGTCTCAAAACCTACTTCATTTTACATTGATACATTTGGCACGAATACAATAGATGAAGAGAGGATTAAAGGG
TTTATTGAAGGTAACATAGATTTATCAACTAAAGGCATAATAAGACATTTATCACTTAATCGTCCTATATATAAACGTAC
AGCCTGCTATGGGCATTTTGGTAAAAAGTTAGAAAGTGATGGTGGATTTTCTTGGGAAGGTGTGGACTTATCTACTGATT
TTTGTAAAGAATTTAATATAGAAGAAGAAAAGAGAGAATTTCTTTAA

Upstream 100 bases:

>100_bases
TGGTTAGAGCGCACGCCTGATAAGCGTGAGGTCGGAGGTTCAACTCTTCCCAGACCCACCATTTAAATTTGCAATAGTTT
TGATATCGTGTAACTAAACG

Downstream 100 bases:

>100_bases
ACTATTAAACTCTTTTATTTCTAGCTTGATACTGATATAATAACTTATTATAAATTGTAAGTAACTTTTTAGTAACCATA
CTAGTTTTAATATTTATGAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase 2; MAT 2; Methionine adenosyltransferase 2 [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MVLHKNSVTSESVAAGHPDKVADQISDAILDEYLFNDSSSRTAIETLVTKDNVIIAGEVFGPNIENSKIESIVRNTIKDI
GYEHDGFHWETVKVNILLHEQSNDIAIGVDQGAGDQGIMYGYATIETESLMPAPIFYAHSILRNIMSMVKEVKLGPDAKS
QITLAYENNLPVRAESIVVSIQHLEDLSQSKVKEIIYPYIASSLPKGWMCPKENLLVNPTGRFVIGGPVGDCGLTGRKIM
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKPTSFYIDTFGTNTIDEERIKG
FIEGNIDLSTKGIIRHLSLNRPIYKRTACYGHFGKKLESDGGFSWEGVDLSTDFCKEFNIEEEKREFL

Sequences:

>Translated_388_residues
MVLHKNSVTSESVAAGHPDKVADQISDAILDEYLFNDSSSRTAIETLVTKDNVIIAGEVFGPNIENSKIESIVRNTIKDI
GYEHDGFHWETVKVNILLHEQSNDIAIGVDQGAGDQGIMYGYATIETESLMPAPIFYAHSILRNIMSMVKEVKLGPDAKS
QITLAYENNLPVRAESIVVSIQHLEDLSQSKVKEIIYPYIASSLPKGWMCPKENLLVNPTGRFVIGGPVGDCGLTGRKIM
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKPTSFYIDTFGTNTIDEERIKG
FIEGNIDLSTKGIIRHLSLNRPIYKRTACYGHFGKKLESDGGFSWEGVDLSTDFCKEFNIEEEKREFL
>Mature_388_residues
MVLHKNSVTSESVAAGHPDKVADQISDAILDEYLFNDSSSRTAIETLVTKDNVIIAGEVFGPNIENSKIESIVRNTIKDI
GYEHDGFHWETVKVNILLHEQSNDIAIGVDQGAGDQGIMYGYATIETESLMPAPIFYAHSILRNIMSMVKEVKLGPDAKS
QITLAYENNLPVRAESIVVSIQHLEDLSQSKVKEIIYPYIASSLPKGWMCPKENLLVNPTGRFVIGGPVGDCGLTGRKIM
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKPTSFYIDTFGTNTIDEERIKG
FIEGNIDLSTKGIIRHLSLNRPIYKRTACYGHFGKKLESDGGFSWEGVDLSTDFCKEFNIEEEKREFL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=375, Percent_Identity=45.8666666666667, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=45.3333333333333, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1789311, Length=379, Percent_Identity=50.131926121372, Blast_Score=356, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=46.2140992167102, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17538494, Length=383, Percent_Identity=45.4308093994778, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=45.0666666666667, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25145633, Length=375, Percent_Identity=45.3333333333333, Blast_Score=290, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=42.5196850393701, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI32565911, Length=331, Percent_Identity=45.619335347432, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI25145635, Length=331, Percent_Identity=44.4108761329305, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI32565903, Length=297, Percent_Identity=46.4646464646465, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=43.3734939759036, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=375, Percent_Identity=44, Blast_Score=281, Evalue=9e-77,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=43.7333333333333, Blast_Score=280, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24580527, Length=375, Percent_Identity=44.8, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24580525, Length=375, Percent_Identity=44.8, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45552159, Length=375, Percent_Identity=44.8, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24580529, Length=375, Percent_Identity=44.8, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24580537, Length=375, Percent_Identity=44.8, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24580533, Length=375, Percent_Identity=44.8, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24580535, Length=375, Percent_Identity=44.8, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24580531, Length=375, Percent_Identity=44.8, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24580541, Length=365, Percent_Identity=43.8356164383562, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=41.025641025641, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42765; Mature: 42765

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLHKNSVTSESVAAGHPDKVADQISDAILDEYLFNDSSSRTAIETLVTKDNVIIAGEVF
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEEEEEC
GPNIENSKIESIVRNTIKDIGYEHDGFHWETVKVNILLHEQSNDIAIGVDQGAGDQGIMY
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCEEEEECCCCCCCCEEE
GYATIETESLMPAPIFYAHSILRNIMSMVKEVKLGPDAKSQITLAYENNLPVRAESIVVS
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEHHHHEEE
IQHLEDLSQSKVKEIIYPYIASSLPKGWMCPKENLLVNPTGRFVIGGPVGDCGLTGRKIM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCEEE
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKP
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSFYIDTFGTNTIDEERIKGFIEGNIDLSTKGIIRHLSLNRPIYKRTACYGHFGKKLESD
CEEEEECCCCCCCCHHHHCEEEECCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
GGFSWEGVDLSTDFCKEFNIEEEKREFL
CCCEECCCCCCHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MVLHKNSVTSESVAAGHPDKVADQISDAILDEYLFNDSSSRTAIETLVTKDNVIIAGEVF
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEEEEEC
GPNIENSKIESIVRNTIKDIGYEHDGFHWETVKVNILLHEQSNDIAIGVDQGAGDQGIMY
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCEEEEECCCCCCCCEEE
GYATIETESLMPAPIFYAHSILRNIMSMVKEVKLGPDAKSQITLAYENNLPVRAESIVVS
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEHHHHEEE
IQHLEDLSQSKVKEIIYPYIASSLPKGWMCPKENLLVNPTGRFVIGGPVGDCGLTGRKIM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCEEE
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKP
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSFYIDTFGTNTIDEERIKGFIEGNIDLSTKGIIRHLSLNRPIYKRTACYGHFGKKLESD
CEEEEECCCCCCCCHHHHCEEEECCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
GGFSWEGVDLSTDFCKEFNIEEEKREFL
CCCEECCCCCCHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA