Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 190571516

GI number: 190571516

Start: 1220405

End: 1221448

Strand: Direct

Name: pheS

Synonym: WPa_1135

Alternate gene names: 190571516

Gene position: 1220405-1221448 (Clockwise)

Preceding gene: 190571512

Following gene: 190571517

Centisome position: 82.32

GC content: 35.44

Gene sequence:

>1044_bases
GTGAATAAAGAGCTAATAAGTGAAATACCTTTACTTGAAGATAAGGCAGTTTCTGAGATTGAAAATGCTGCTTCTTTGAA
GGATTTAGAAAAAGTTAGGTTGTCATACTTGGGGAGAAAGGGTGTAGTAAAAGCTTATTTCGATGACTTAAAGAATATAG
ATGATGCAGGAGAGAAACGCGACCTAGGTGCAGTGATTAATGTTTTACGCAATAAGATAGACCAGCTTATAACAAGCAAA
GAAAATGAACTGAAAGATAAAGAAGTTAAGTTAAAACTGCAAAATGAAGCAGTTGATATCACACTGCCTGTCAGACCAGA
AAGAATTGGCAAGATTCATCCACTCAGTAAAGTTATAAGTGAAGTGAAGCTCATTTTTGCGCATATGGGCTTTAAAGCAG
TTGATGGTCCTGATATTGAAGATGAATTTCACGTATTTGATGCATTAAATACTCCAAGTCACCACCCTGCACGAGAGGAG
CAAGATACCTTCTATTTAAGGAATAAAATAAATGATAAAAGAATGGTGCTGCGCACTCATACCTCATCTGTGCAGATTAG
AACCATGGAAAAGACAAAAACTTTTCCAATTAAAATAGTGGCTGCAGGCAGGGTATACAGAAATGACTTTGATGCAACTC
ACACTCCTATGTTTCATCAGATAGAAGGACTTTATGTTAATGAAAATGTCAATATGGGCCAATTAAAATTTACTATTCAT
TGCTTCCTTAGTAAATTTTTCGGAGATAAGGGTCTAAGAATACGCTTTCGTAATAGTTTTTTCCCTTTTACTGAACCTTC
TGCGGAAGTGGACATAAGCTATAAAGATAGTAAATGGATTGAAGTACTTGGATGTGGTATGGTGCATCCAAATGTCTTTA
AAAATGTTGGAATAGACCATACTAAATACAGTGGCTTTGCATTTGGGATTGGTATAGAAAGGCTAGCAATGCTGAAATAT
CAAATTAGTGATCTAAGGAGCTTTTACGACAATAGAGTTAGTTGGCTCAACCATTATGGTTTTCATTTTTCGTCTTTAAG
ATAA

Upstream 100 bases:

>100_bases
ATAGAGATCAGCTACTCTGATGATAACTTTTTATCCAAATAGTTTTATTGATGATATTAAAGATTGCACTATAATGAAAG
TTTAAGCTAGCGTTTTTAAG

Downstream 100 bases:

>100_bases
GTGACAAATAAATCTTATACATTTGTTGTACTTGCTGCTGGGCATAGTGAACGAATGAATTCAAGCTTGCCTAAGGTTCT
GCACAAAATAGGTAATTTCT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNKELISEIPLLEDKAVSEIENAASLKDLEKVRLSYLGRKGVVKAYFDDLKNIDDAGEKRDLGAVINVLRNKIDQLITSK
ENELKDKEVKLKLQNEAVDITLPVRPERIGKIHPLSKVISEVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREE
QDTFYLRNKINDKRMVLRTHTSSVQIRTMEKTKTFPIKIVAAGRVYRNDFDATHTPMFHQIEGLYVNENVNMGQLKFTIH
CFLSKFFGDKGLRIRFRNSFFPFTEPSAEVDISYKDSKWIEVLGCGMVHPNVFKNVGIDHTKYSGFAFGIGIERLAMLKY
QISDLRSFYDNRVSWLNHYGFHFSSLR

Sequences:

>Translated_347_residues
MNKELISEIPLLEDKAVSEIENAASLKDLEKVRLSYLGRKGVVKAYFDDLKNIDDAGEKRDLGAVINVLRNKIDQLITSK
ENELKDKEVKLKLQNEAVDITLPVRPERIGKIHPLSKVISEVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREE
QDTFYLRNKINDKRMVLRTHTSSVQIRTMEKTKTFPIKIVAAGRVYRNDFDATHTPMFHQIEGLYVNENVNMGQLKFTIH
CFLSKFFGDKGLRIRFRNSFFPFTEPSAEVDISYKDSKWIEVLGCGMVHPNVFKNVGIDHTKYSGFAFGIGIERLAMLKY
QISDLRSFYDNRVSWLNHYGFHFSSLR
>Mature_347_residues
MNKELISEIPLLEDKAVSEIENAASLKDLEKVRLSYLGRKGVVKAYFDDLKNIDDAGEKRDLGAVINVLRNKIDQLITSK
ENELKDKEVKLKLQNEAVDITLPVRPERIGKIHPLSKVISEVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREE
QDTFYLRNKINDKRMVLRTHTSSVQIRTMEKTKTFPIKIVAAGRVYRNDFDATHTPMFHQIEGLYVNENVNMGQLKFTIH
CFLSKFFGDKGLRIRFRNSFFPFTEPSAEVDISYKDSKWIEVLGCGMVHPNVFKNVGIDHTKYSGFAFGIGIERLAMLKY
QISDLRSFYDNRVSWLNHYGFHFSSLR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=268, Percent_Identity=33.5820895522388, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI5729820, Length=220, Percent_Identity=31.8181818181818, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788007, Length=334, Percent_Identity=47.9041916167665, Blast_Score=332, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=31.021897810219, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=31.021897810219, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=246, Percent_Identity=30.8943089430894, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=30.7116104868914, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=269, Percent_Identity=26.3940520446097, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18858079, Length=363, Percent_Identity=26.1707988980716, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17137424, Length=243, Percent_Identity=29.2181069958848, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_WOLPP (B3CMZ6)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975874.1
- EnsemblBacteria:   EBWOLT00000000105
- GeneID:   6385518
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_1135
- GeneTree:   EBGT00050000031096
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39986; Mature: 39986

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELISEIPLLEDKAVSEIENAASLKDLEKVRLSYLGRKGVVKAYFDDLKNIDDAGEKR
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
DLGAVINVLRNKIDQLITSKENELKDKEVKLKLQNEAVDITLPVRPERIGKIHPLSKVIS
HHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCEEEEEECCCHHHCCCCCHHHHHHH
EVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREEQDTFYLRNKINDKRMVLRTH
HHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEC
TSSVQIRTMEKTKTFPIKIVAAGRVYRNDFDATHTPMFHQIEGLYVNENVNMGQLKFTIH
CCCEEEEEECCCCCCCEEEEEECEEECCCCCCCCCCHHHEECCEEEECCCCCCCEEHHHH
CFLSKFFGDKGLRIRFRNSFFPFTEPSAEVDISYKDSKWIEVLGCGMVHPNVFKNVGIDH
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHCCCCC
TKYSGFAFGIGIERLAMLKYQISDLRSFYDNRVSWLNHYGFHFSSLR
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
>Mature Secondary Structure
MNKELISEIPLLEDKAVSEIENAASLKDLEKVRLSYLGRKGVVKAYFDDLKNIDDAGEKR
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHH
DLGAVINVLRNKIDQLITSKENELKDKEVKLKLQNEAVDITLPVRPERIGKIHPLSKVIS
HHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCEEEEEECCCHHHCCCCCHHHHHHH
EVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREEQDTFYLRNKINDKRMVLRTH
HHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEC
TSSVQIRTMEKTKTFPIKIVAAGRVYRNDFDATHTPMFHQIEGLYVNENVNMGQLKFTIH
CCCEEEEEECCCCCCCEEEEEECEEECCCCCCCCCCHHHEECCEEEECCCCCCCEEHHHH
CFLSKFFGDKGLRIRFRNSFFPFTEPSAEVDISYKDSKWIEVLGCGMVHPNVFKNVGIDH
HHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHCCCCC
TKYSGFAFGIGIERLAMLKYQISDLRSFYDNRVSWLNHYGFHFSSLR
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA