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The map label for this gene is glyS [H]

Identifier: 190571503

GI number: 190571503

Start: 1202879

End: 1204987

Strand: Reverse

Name: glyS [H]

Synonym: WPa_1121

Alternate gene names: 190571503

Gene position: 1204987-1202879 (Counterclockwise)

Preceding gene: 190571504

Following gene: 190571502

Centisome position: 81.28

GC content: 33.19

Gene sequence:

>2109_bases
ATGTTGTCACAATTATTGTTTGAATGTCTTTCAGAAGAAATTCCATCAAGAATGCAGAATTCATCTGCAGCTCAGGTTAA
GAGCTATATCACTAATGCTTTTAGTAAAAATAATGTGAAATTTGCATCTATAGAAGTTTATGTGACTGCACGTCGCATTG
CTCTTTTTATTGATGGCATAAGTGCTTTAGAGCTAAAAGATTCTAACAATGAAATTAAAGGACCAAGAGTTAATGCACCG
AAAAGTGCTATCGAAGGTTTTTTGAGAAAAAATCGGAAAGGTGAAGAAGATTTACTCATTCGCAAAGTAAATGATGAAGA
TTTTTACTTCATTAAGAGAGAAAGCTGCTTATTTAACATCAACGGGTTTCTCAAAAATCAACTGGAGGAAATGCTAAAAA
ACTTTTCTTGGCCAAAGAGTATGAGATGGAGTGAAAGAAAAGAGAGATGGGTTAGGCCAATTAAAAATATTCTATGTATT
TTAAATGACGAAATAATACCTATATCTTTTGCAGGGGTTACAGCATGCAACGTGACGTATGGTCATAGGTTTCTTTCAGG
TGATGCAGTACTTACTGTTAAAACGCCCAAAGACTATTTTGAATTGTTAGAAAAAAATAATGTCATTCTCCAGTTGGACA
AAAGAAAGCAATTTATACTAGATCAGATTAATAAGTTTACAAAAGAGGAGAACTTACAACTTGAAAAAAATGATTATTTA
CTTAATGAATTGGCAGGGCTTATAGAGTGGCCAATAGTATTATTTGGTAAAGTAAAATCATCAGAATTACCGAAGAAAGT
AATACTTAGTATAATTAATACGCAGCAAAAGTATCTTGCTTTAAGTGATGGACAGAAAATATCACATTTTGTTACTGTTG
TAAATGTTAACAACAATGAAGTTGTCAAAGGCCATGAAAGAATATTAGAAGCACGTCTTGCTGATGCCCAGTTTTTGATA
TCTCAAGACAAGAAGGAAAACCTAGATTACTATGTCAAAAAATTGAGCTCTATTTCATTTCATGCTTCGCTCGGTAGCGT
AGAAGAAAAAGTGAAGCGTATTATCGCTCTTTCAAAGTATATAGCGATATTTATTCCACATGCTTCATTAATTAAAGTTG
AACGTGCTGCATATTTAGCAAAGGCAGATCTTGCAACGTTGATAGTGAAAGAATTTCCGGGATTGCAAGGAGTAATGAGT
GGATACTACGCTTCTTATTTTCAAGAGGATAAAGAAGTAGTGGAAGCTATAACTGAGCACTATAAGCCAATTGGATCGGA
CCAAGAATGCCCTAGATCTCCTACTGCGATTGCTGTAGCTATTGCAGACAAAATGGATAGTTTGGTTGGCTTAATTGCAG
CAGGTGAGAAAATCTCTGGTTCGTATGATCAGTTTGGTTTGCGTAGAATGACAATTGGTATAATTAGAACAATACTTGAA
AATAATTTGCATATTCCAATTAGATTGCTGATAGATAAGTCAGTATCTTTATGTTCAAGGCTTCTATTGAATAAAAATAT
AACTCCAGTTGATAAGCCAAGTGAAAAACAAATTTTAGAACTAGTATTTAAATTCTGCTTAGAAAGGTTCAAGATTATTT
TAAAAAATAGAAATATAAGGCAAGATGTTGTAGATTCAATACTATATAAAGTCGATATTAATGATCTGCTGAAAGCGGAA
AAGCAAACTGTGATATTGGATCGTTATCTTAGTACAAAAGAAGGTGAACAGGTTCTAAGCACTTATAAAAGAGCCAGTAA
CATGATGAGCAAAGTGAGAAAAAGTGATGGCACTACTTATAATGCATCTTACAGTAAGAAGTTTTTGATTGAAGATGAGG
AGATTGCGCTATCAAATTGTGCTATAGCTGCTTGTAAAAACATCAAACAAGCGATAGAAAATAACGACTTTAATACAGCG
CTTGATGAACTTGCTCGTTTTGCTCCATTTATCAATCAATTTATGGACAGTGTAAAGATTAACTGTGATTCTAATGAGCT
TAGAATAAACAGGTTATCTTTGCTTGCAAATGTTGTTTCTACCTTTCATTTAGTAGCAGAGTTTAACCTTATACAGGTTA
AACAATTGATAAATGCTCAGGCAATATAA

Upstream 100 bases:

>100_bases
TGCAAGAGGTGTGCTTGGTGTGAATGAACGTACAGCCTATATTGGTAGAGTGAGAGAGTTAACAAAAAAATGCTGTGAAT
TATATAGAGCAAAGTAGAAT

Downstream 100 bases:

>100_bases
GGAACAAATCAAATCATCTCCACAATCCTGTGGCGTTTATAAGATGATTGGAGATAAGAGTAAGGTTTTATACATTGGTA
AAGCAAAAAACTTGAAGTCG

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MLSQLLFECLSEEIPSRMQNSSAAQVKSYITNAFSKNNVKFASIEVYVTARRIALFIDGISALELKDSNNEIKGPRVNAP
KSAIEGFLRKNRKGEEDLLIRKVNDEDFYFIKRESCLFNINGFLKNQLEEMLKNFSWPKSMRWSERKERWVRPIKNILCI
LNDEIIPISFAGVTACNVTYGHRFLSGDAVLTVKTPKDYFELLEKNNVILQLDKRKQFILDQINKFTKEENLQLEKNDYL
LNELAGLIEWPIVLFGKVKSSELPKKVILSIINTQQKYLALSDGQKISHFVTVVNVNNNEVVKGHERILEARLADAQFLI
SQDKKENLDYYVKKLSSISFHASLGSVEEKVKRIIALSKYIAIFIPHASLIKVERAAYLAKADLATLIVKEFPGLQGVMS
GYYASYFQEDKEVVEAITEHYKPIGSDQECPRSPTAIAVAIADKMDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRTILE
NNLHIPIRLLIDKSVSLCSRLLLNKNITPVDKPSEKQILELVFKFCLERFKIILKNRNIRQDVVDSILYKVDINDLLKAE
KQTVILDRYLSTKEGEQVLSTYKRASNMMSKVRKSDGTTYNASYSKKFLIEDEEIALSNCAIAACKNIKQAIENNDFNTA
LDELARFAPFINQFMDSVKINCDSNELRINRLSLLANVVSTFHLVAEFNLIQVKQLINAQAI

Sequences:

>Translated_702_residues
MLSQLLFECLSEEIPSRMQNSSAAQVKSYITNAFSKNNVKFASIEVYVTARRIALFIDGISALELKDSNNEIKGPRVNAP
KSAIEGFLRKNRKGEEDLLIRKVNDEDFYFIKRESCLFNINGFLKNQLEEMLKNFSWPKSMRWSERKERWVRPIKNILCI
LNDEIIPISFAGVTACNVTYGHRFLSGDAVLTVKTPKDYFELLEKNNVILQLDKRKQFILDQINKFTKEENLQLEKNDYL
LNELAGLIEWPIVLFGKVKSSELPKKVILSIINTQQKYLALSDGQKISHFVTVVNVNNNEVVKGHERILEARLADAQFLI
SQDKKENLDYYVKKLSSISFHASLGSVEEKVKRIIALSKYIAIFIPHASLIKVERAAYLAKADLATLIVKEFPGLQGVMS
GYYASYFQEDKEVVEAITEHYKPIGSDQECPRSPTAIAVAIADKMDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRTILE
NNLHIPIRLLIDKSVSLCSRLLLNKNITPVDKPSEKQILELVFKFCLERFKIILKNRNIRQDVVDSILYKVDINDLLKAE
KQTVILDRYLSTKEGEQVLSTYKRASNMMSKVRKSDGTTYNASYSKKFLIEDEEIALSNCAIAACKNIKQAIENNDFNTA
LDELARFAPFINQFMDSVKINCDSNELRINRLSLLANVVSTFHLVAEFNLIQVKQLINAQAI
>Mature_702_residues
MLSQLLFECLSEEIPSRMQNSSAAQVKSYITNAFSKNNVKFASIEVYVTARRIALFIDGISALELKDSNNEIKGPRVNAP
KSAIEGFLRKNRKGEEDLLIRKVNDEDFYFIKRESCLFNINGFLKNQLEEMLKNFSWPKSMRWSERKERWVRPIKNILCI
LNDEIIPISFAGVTACNVTYGHRFLSGDAVLTVKTPKDYFELLEKNNVILQLDKRKQFILDQINKFTKEENLQLEKNDYL
LNELAGLIEWPIVLFGKVKSSELPKKVILSIINTQQKYLALSDGQKISHFVTVVNVNNNEVVKGHERILEARLADAQFLI
SQDKKENLDYYVKKLSSISFHASLGSVEEKVKRIIALSKYIAIFIPHASLIKVERAAYLAKADLATLIVKEFPGLQGVMS
GYYASYFQEDKEVVEAITEHYKPIGSDQECPRSPTAIAVAIADKMDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRTILE
NNLHIPIRLLIDKSVSLCSRLLLNKNITPVDKPSEKQILELVFKFCLERFKIILKNRNIRQDVVDSILYKVDINDLLKAE
KQTVILDRYLSTKEGEQVLSTYKRASNMMSKVRKSDGTTYNASYSKKFLIEDEEIALSNCAIAACKNIKQAIENNDFNTA
LDELARFAPFINQFMDSVKINCDSNELRINRLSLLANVVSTFHLVAEFNLIQVKQLINAQAI

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=720, Percent_Identity=29.0277777777778, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 79904; Mature: 79904

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQLLFECLSEEIPSRMQNSSAAQVKSYITNAFSKNNVKFASIEVYVTARRIALFIDGI
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEHEEEEHEECCC
SALELKDSNNEIKGPRVNAPKSAIEGFLRKNRKGEEDLLIRKVNDEDFYFIKRESCLFNI
EEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCEEEEC
NGFLKNQLEEMLKNFSWPKSMRWSERKERWVRPIKNILCILNDEIIPISFAGVTACNVTY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEC
GHRFLSGDAVLTVKTPKDYFELLEKNNVILQLDKRKQFILDQINKFTKEENLQLEKNDYL
CCEEECCCEEEEEECCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCEECCCHHH
LNELAGLIEWPIVLFGKVKSSELPKKVILSIINTQQKYLALSDGQKISHFVTVVNVNNNE
HHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCHHHEEEECCCCEEEEEEEEEECCCCC
VVKGHERILEARLADAQFLISQDKKENLDYYVKKLSSISFHASLGSVEEKVKRIIALSKY
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IAIFIPHASLIKVERAAYLAKADLATLIVKEFPGLQGVMSGYYASYFQEDKEVVEAITEH
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YKPIGSDQECPRSPTAIAVAIADKMDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRTILE
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
NNLHIPIRLLIDKSVSLCSRLLLNKNITPVDKPSEKQILELVFKFCLERFKIILKNRNIR
CCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
QDVVDSILYKVDINDLLKAEKQTVILDRYLSTKEGEQVLSTYKRASNMMSKVRKSDGTTY
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
NASYSKKFLIEDEEIALSNCAIAACKNIKQAIENNDFNTALDELARFAPFINQFMDSVKI
ECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
NCDSNELRINRLSLLANVVSTFHLVAEFNLIQVKQLINAQAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSQLLFECLSEEIPSRMQNSSAAQVKSYITNAFSKNNVKFASIEVYVTARRIALFIDGI
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEHEEEEHEECCC
SALELKDSNNEIKGPRVNAPKSAIEGFLRKNRKGEEDLLIRKVNDEDFYFIKRESCLFNI
EEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCEEEEC
NGFLKNQLEEMLKNFSWPKSMRWSERKERWVRPIKNILCILNDEIIPISFAGVTACNVTY
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEC
GHRFLSGDAVLTVKTPKDYFELLEKNNVILQLDKRKQFILDQINKFTKEENLQLEKNDYL
CCEEECCCEEEEEECCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCEECCCHHH
LNELAGLIEWPIVLFGKVKSSELPKKVILSIINTQQKYLALSDGQKISHFVTVVNVNNNE
HHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCHHHEEEECCCCEEEEEEEEEECCCCC
VVKGHERILEARLADAQFLISQDKKENLDYYVKKLSSISFHASLGSVEEKVKRIIALSKY
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IAIFIPHASLIKVERAAYLAKADLATLIVKEFPGLQGVMSGYYASYFQEDKEVVEAITEH
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YKPIGSDQECPRSPTAIAVAIADKMDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRTILE
CCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
NNLHIPIRLLIDKSVSLCSRLLLNKNITPVDKPSEKQILELVFKFCLERFKIILKNRNIR
CCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
QDVVDSILYKVDINDLLKAEKQTVILDRYLSTKEGEQVLSTYKRASNMMSKVRKSDGTTY
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
NASYSKKFLIEDEEIALSNCAIAACKNIKQAIENNDFNTALDELARFAPFINQFMDSVKI
ECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
NCDSNELRINRLSLLANVVSTFHLVAEFNLIQVKQLINAQAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA