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The map label for this gene is rlmB [H]

Identifier: 190571500

GI number: 190571500

Start: 1199715

End: 1200482

Strand: Reverse

Name: rlmB [H]

Synonym: WPa_1118

Alternate gene names: 190571500

Gene position: 1200482-1199715 (Counterclockwise)

Preceding gene: 190571502

Following gene: 190571497

Centisome position: 80.98

GC content: 33.33

Gene sequence:

>768_bases
ATGAAATCATCAAAAGCTAATGAAAACTGTTGGATATATGGAAAGCACACTTGTATTTCAGCATTAAGAAACAAAAATAG
GCGGTGTATAGAACTGTTAGTAACAGAAAATTTCTACAGAGAATTTGAGAAAGAAATTAAACAATGTGCAGATAGTAAGG
GCATTAAACCTCGACTGGTAGAGAACAAAATGTTCAATGATGTTTTACCCAAAGGTGTCAATCATCAAGGAATTGCTTTA
AAAGTTGCTCCTATTTTTCACAGCTTAAATATCGAAGAAATAGCTGAAGGTTCAGGTGACAGCTCTACTATAGTCATTTT
AGATCAAATTACTGATACACACAATATAGGGTCGATTTTAAGAACCTCAGCTTGTTTCAACATTGATGCACTGGTTTTAC
CACATCACCATTCACCGAGCGAAAATGCATCTATCGCAAAAGCAGCAAGTGGAGCACTGGATATTGTTCCTCTAATATAC
GTTACAAACATAGTAAAAACGATGGAGTCTTTAAAAAAGATAGGCTACTGGTGTTATGGGTTTGATTGCAATGCAAAGGA
AAATATAGATGAAATAAAGAATTTTGGGCAAAAAAGAGTAATTATTTTTGGTTCTGAAGAGAAAGGAATGCGGAGATTAG
TGAAAGAAAGTTGTGATTATCTTTTAAAAATTCCAATGTCAAACGTAATTGATAGTCTAAATGTTTCAAACGCAGCAGCA
ATAGGACTATATTCCATTTACATTAAAACAAAAATCACTGCAAATTAG

Upstream 100 bases:

>100_bases
TCTGACATAAATTCATTAATTGAACTGCATAATTTTACAACAATATATAAACACAGTAAAATATTGCTTATTCTATAAAA
AACTGATTTTCAATAATGAG

Downstream 100 bases:

>100_bases
TTGCAATAAAACAAGGATGTCATTCCAGCATGTGATACTGGAATGACAACAGTGAATGACATCTTGCGATGTCATTCACT
AGCACTTACATTACCAATCC

Product: RNA methyltransferase, TrmH family, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKSSKANENCWIYGKHTCISALRNKNRRCIELLVTENFYREFEKEIKQCADSKGIKPRLVENKMFNDVLPKGVNHQGIAL
KVAPIFHSLNIEEIAEGSGDSSTIVILDQITDTHNIGSILRTSACFNIDALVLPHHHSPSENASIAKAASGALDIVPLIY
VTNIVKTMESLKKIGYWCYGFDCNAKENIDEIKNFGQKRVIIFGSEEKGMRRLVKESCDYLLKIPMSNVIDSLNVSNAAA
IGLYSIYIKTKITAN

Sequences:

>Translated_255_residues
MKSSKANENCWIYGKHTCISALRNKNRRCIELLVTENFYREFEKEIKQCADSKGIKPRLVENKMFNDVLPKGVNHQGIAL
KVAPIFHSLNIEEIAEGSGDSSTIVILDQITDTHNIGSILRTSACFNIDALVLPHHHSPSENASIAKAASGALDIVPLIY
VTNIVKTMESLKKIGYWCYGFDCNAKENIDEIKNFGQKRVIIFGSEEKGMRRLVKESCDYLLKIPMSNVIDSLNVSNAAA
IGLYSIYIKTKITAN
>Mature_255_residues
MKSSKANENCWIYGKHTCISALRNKNRRCIELLVTENFYREFEKEIKQCADSKGIKPRLVENKMFNDVLPKGVNHQGIAL
KVAPIFHSLNIEEIAEGSGDSSTIVILDQITDTHNIGSILRTSACFNIDALVLPHHHSPSENASIAKAASGALDIVPLIY
VTNIVKTMESLKKIGYWCYGFDCNAKENIDEIKNFGQKRVIIFGSEEKGMRRLVKESCDYLLKIPMSNVIDSLNVSNAAA
IGLYSIYIKTKITAN

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=235, Percent_Identity=32.7659574468085, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324775, Length=284, Percent_Identity=23.5915492957746, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28454; Mature: 28454

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSKANENCWIYGKHTCISALRNKNRRCIELLVTENFYREFEKEIKQCADSKGIKPRLV
CCCCCCCCCCEEEEHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ENKMFNDVLPKGVNHQGIALKVAPIFHSLNIEEIAEGSGDSSTIVILDQITDTHNIGSIL
HHHHHHHHCCCCCCCCCEEEEEEHHHHHCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
RTSACFNIDALVLPHHHSPSENASIAKAASGALDIVPLIYVTNIVKTMESLKKIGYWCYG
HHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
FDCNAKENIDEIKNFGQKRVIIFGSEEKGMRRLVKESCDYLLKIPMSNVIDSLNVSNAAA
ECCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHEEECCHHHHHHHCCCCCHHH
IGLYSIYIKTKITAN
HEEEEEEEEEEEECC
>Mature Secondary Structure
MKSSKANENCWIYGKHTCISALRNKNRRCIELLVTENFYREFEKEIKQCADSKGIKPRLV
CCCCCCCCCCEEEEHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ENKMFNDVLPKGVNHQGIALKVAPIFHSLNIEEIAEGSGDSSTIVILDQITDTHNIGSIL
HHHHHHHHCCCCCCCCCEEEEEEHHHHHCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
RTSACFNIDALVLPHHHSPSENASIAKAASGALDIVPLIYVTNIVKTMESLKKIGYWCYG
HHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
FDCNAKENIDEIKNFGQKRVIIFGSEEKGMRRLVKESCDYLLKIPMSNVIDSLNVSNAAA
ECCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHEEECCHHHHHHHCCCCCHHH
IGLYSIYIKTKITAN
HEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]