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The map label for this gene is dagA [H]

Identifier: 190571493

GI number: 190571493

Start: 1187617

End: 1189083

Strand: Direct

Name: dagA [H]

Synonym: WPa_1109

Alternate gene names: 190571493

Gene position: 1187617-1189083 (Clockwise)

Preceding gene: 190571492

Following gene: 190571494

Centisome position: 80.11

GC content: 37.15

Gene sequence:

>1467_bases
ATGATTTATAGATTGCTTTGCATGATGTTATTTTGCCTATCGTTTAACGATGTATACGCTGCTTCAGTTTTTCATAAAAG
TTATGACACTGCATTAAATGGAGTAAGCAACTTCATGAATGAAGTGTTGTTTTTCAAGATCTTTCACGTACCATTTATAA
TTCTTTTATTGGCCTTTGGTTATATATTTCTAACACTACGTTTTAGATTTCTCAACATAAGGATGTTTAAGCATGCGTTC
GCTATTTTATGTGGAAAATATGACACAGATCATCATGATGGTCACATTACGCATTTTCAGGCATTTGTAACCGCGCTATC
AAGCACAGTAGGTCTTGGAACTGTTGCTGGTGTTGCAATTGCAATTTCAATGGGAGGGCCAGGAGCGGTACCTTGGATGA
TGATCACAGGCTTTTTTGGTATGTCTGCAAAATTCGCTGAAGTGACTTTAGCATTCAGGCACAGAACAGAAGGTCAAGAG
CAGTTATTTAGTGGTCCTTTTCAATATATAAGAAATGGGCTAGAGGAATTTGGATTTAAAAAACTTGGTATCGTACTGGC
TGCAATATATGCAGCGGTTTTTGTATTATCAGGTCTTGGTGGAAGTGTAGCGTTTCAAACTAACCAAATGGTTTCAATAT
TATCTGGTTATTCAAATTGGATAGATGGTCATCCTTGGTTTCTCTCTTCCATAATTTCTACGTTACTTGCTCTGGTGATT
ATCGGTGGAATCAGGAGAATAGCTAAAGTCTCTTCTGCGCTGGTGCCTATTATGTCACTCATATATATTTTGAGTTGTTT
CACGATTATTGCATTCAATATTCACAATTTTAGCAATACATTAAAAATATTGTTTTCCAGTATGATAGACTTCAATGCTG
TTGGTGGTGGAGTAGTAGGAGCATTTGTCGCTGGGATTCAAAGGGCAATTTTTGCCAGTGAAGCAGGTGTTGGTTCTGCT
GCAATTACTCACGCTGTAGCTAAAGATGAGGAGCCGGTTAGGACTGGTCTAGTTGCTATGATAGAACCATGCTTCGATAC
AATGTTAATTTGTTGTTTGACAGGAATAACAATAGTAATAACAGGCGCATACCAGACTGGCATTGGCGAGGGAATATTGA
TCACCCAAAAAGCATTTGAAACAGTTTCTTCTTGGTTTCCTATACTTTTAACCATCGCTGCGCCTTTGTTTGCGTTTTCC
TCAGTAATTTCATTTGTATATTGTTGTGAAATGGGATGGCTGTATTTGTTTGGTGTAAAAAGTGTAGTAATATATCGTAT
AGCGGTGGTGGTAGTTGCATTTTTGTCAGGTTTGTCTAAAGATATAATGGCTATTGCTAATATTGGTGGGGCTTTATTCT
CTTGTTTAGCACTCATCAATATGACAGCACTTATACTGTTTAGCAATCAGATTAATGATGAAGTTCAATGCTATCTAAAA
AGACTGAGGAATAACAAGATCAACTAG

Upstream 100 bases:

>100_bases
GCCTGTTTATGTGCCTTATGATTCCAAATGCTGTTGCAATATATCTACTGAGAAGGGAAGTCTTAAATACAATAGATTCT
TATTACAAAAGGTATTAAAC

Downstream 100 bases:

>100_bases
ATTTTTAGTATAATGGAGAAATATATAATTCTTATATTATCTTACGTAATCGGTTCAATACCGTTTAGCTTAATTATTGC
AAAAATAAATGGTATAAATT

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MIYRLLCMMLFCLSFNDVYAASVFHKSYDTALNGVSNFMNEVLFFKIFHVPFIILLLAFGYIFLTLRFRFLNIRMFKHAF
AILCGKYDTDHHDGHITHFQAFVTALSSTVGLGTVAGVAIAISMGGPGAVPWMMITGFFGMSAKFAEVTLAFRHRTEGQE
QLFSGPFQYIRNGLEEFGFKKLGIVLAAIYAAVFVLSGLGGSVAFQTNQMVSILSGYSNWIDGHPWFLSSIISTLLALVI
IGGIRRIAKVSSALVPIMSLIYILSCFTIIAFNIHNFSNTLKILFSSMIDFNAVGGGVVGAFVAGIQRAIFASEAGVGSA
AITHAVAKDEEPVRTGLVAMIEPCFDTMLICCLTGITIVITGAYQTGIGEGILITQKAFETVSSWFPILLTIAAPLFAFS
SVISFVYCCEMGWLYLFGVKSVVIYRIAVVVVAFLSGLSKDIMAIANIGGALFSCLALINMTALILFSNQINDEVQCYLK
RLRNNKIN

Sequences:

>Translated_488_residues
MIYRLLCMMLFCLSFNDVYAASVFHKSYDTALNGVSNFMNEVLFFKIFHVPFIILLLAFGYIFLTLRFRFLNIRMFKHAF
AILCGKYDTDHHDGHITHFQAFVTALSSTVGLGTVAGVAIAISMGGPGAVPWMMITGFFGMSAKFAEVTLAFRHRTEGQE
QLFSGPFQYIRNGLEEFGFKKLGIVLAAIYAAVFVLSGLGGSVAFQTNQMVSILSGYSNWIDGHPWFLSSIISTLLALVI
IGGIRRIAKVSSALVPIMSLIYILSCFTIIAFNIHNFSNTLKILFSSMIDFNAVGGGVVGAFVAGIQRAIFASEAGVGSA
AITHAVAKDEEPVRTGLVAMIEPCFDTMLICCLTGITIVITGAYQTGIGEGILITQKAFETVSSWFPILLTIAAPLFAFS
SVISFVYCCEMGWLYLFGVKSVVIYRIAVVVVAFLSGLSKDIMAIANIGGALFSCLALINMTALILFSNQINDEVQCYLK
RLRNNKIN
>Mature_488_residues
MIYRLLCMMLFCLSFNDVYAASVFHKSYDTALNGVSNFMNEVLFFKIFHVPFIILLLAFGYIFLTLRFRFLNIRMFKHAF
AILCGKYDTDHHDGHITHFQAFVTALSSTVGLGTVAGVAIAISMGGPGAVPWMMITGFFGMSAKFAEVTLAFRHRTEGQE
QLFSGPFQYIRNGLEEFGFKKLGIVLAAIYAAVFVLSGLGGSVAFQTNQMVSILSGYSNWIDGHPWFLSSIISTLLALVI
IGGIRRIAKVSSALVPIMSLIYILSCFTIIAFNIHNFSNTLKILFSSMIDFNAVGGGVVGAFVAGIQRAIFASEAGVGSA
AITHAVAKDEEPVRTGLVAMIEPCFDTMLICCLTGITIVITGAYQTGIGEGILITQKAFETVSSWFPILLTIAAPLFAFS
SVISFVYCCEMGWLYLFGVKSVVIYRIAVVVVAFLSGLSKDIMAIANIGGALFSCLALINMTALILFSNQINDEVQCYLK
RLRNNKIN

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=462, Percent_Identity=29.8701298701299, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 53236; Mature: 53236

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCC
>Mature Secondary Structure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HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]