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The map label for this gene is nuoI [H]

Identifier: 190571400

GI number: 190571400

Start: 1074877

End: 1075359

Strand: Reverse

Name: nuoI [H]

Synonym: WPa_1008

Alternate gene names: 190571400

Gene position: 1075359-1074877 (Counterclockwise)

Preceding gene: 190571408

Following gene: 190571399

Centisome position: 72.54

GC content: 39.75

Gene sequence:

>483_bases
ATGCTCAAGAAATTAGCTTGGTATTGGTCTTCTGTAGAGTTAATTAAAGGGTTTGTTATTACATTAAAATATATGTTCAA
ACCAAAGGTTACTTTGAGGTATCCTATGGAGAAGGGCCCTTTAAGTCCAAGGTTTCGTGGTGAGCATGCGCTGCGTAGGT
ATCCAAATGGTGAAGAACGATGCATAGCTTGTAAGTTATGCGAAGTTATCTGCCCTGCTCAAGCAATAGTTATTGAAGCA
GAGGAAAGAGAAGACGGTAGTCGCCGCACCACACGCTATGATATTGACATGACAAAATGCATATATTGCGGACTTTGCCA
AGAAGCATGTCCAGTTGATGCAATTGTGGAGGGTCCTAACTTTGAATTTGCTACTGAAACAAGAGAGGAGCTAATGTACA
ATAAAGAAAAGCTATTGCGTAATGGTGAAGTTTGGGAAGAAGCAATTGCACTCAGGTTAAAAAAGAATAGGCCGTATTAT
TAA

Upstream 100 bases:

>100_bases
ATATATTGTATAGAAAAACTACAAAAGTCATCCAGATGAAAATATTTATTTTGAAAACTGATAATATGATTTATAATGAC
TGACACAAGTTTTAAATATT

Downstream 100 bases:

>100_bases
CTAGGTATGTCAACAATTAAAATTTCTTACAGCAAGTATGTAATAGATCTCTTGTACAACTGTTATTTAACTGAAAGATC
AGTAGCTCACCTTTTGGTGT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MLKKLAWYWSSVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEERCIACKLCEVICPAQAIVIEA
EEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEEAIALRLKKNRPYY

Sequences:

>Translated_160_residues
MLKKLAWYWSSVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEERCIACKLCEVICPAQAIVIEA
EEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEEAIALRLKKNRPYY
>Mature_160_residues
MLKKLAWYWSSVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEERCIACKLCEVICPAQAIVIEA
EEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPNFEFATETREELMYNKEKLLRNGEVWEEAIALRLKKNRPYY

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=148, Percent_Identity=75, Blast_Score=239, Evalue=7e-64,
Organism=Escherichia coli, GI1788617, Length=137, Percent_Identity=41.6058394160584, Blast_Score=109, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555194, Length=143, Percent_Identity=75.5244755244755, Blast_Score=233, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17864306, Length=147, Percent_Identity=77.5510204081633, Blast_Score=247, Evalue=2e-66,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18799; Mature: 18799

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLAWYWSSVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEER
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCHH
CIACKLCEVICPAQAIVIEAEEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPN
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEECCC
FEFATETREELMYNKEKLLRNGEVWEEAIALRLKKNRPYY
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLKKLAWYWSSVELIKGFVITLKYMFKPKVTLRYPMEKGPLSPRFRGEHALRRYPNGEER
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCHH
CIACKLCEVICPAQAIVIEAEEREDGSRRTTRYDIDMTKCIYCGLCQEACPVDAIVEGPN
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEECCC
FEFATETREELMYNKEKLLRNGEVWEEAIALRLKKNRPYY
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA