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The map label for this gene is rsmE [H]

Identifier: 190571358

GI number: 190571358

Start: 1030272

End: 1030967

Strand: Reverse

Name: rsmE [H]

Synonym: WPa_0963

Alternate gene names: 190571358

Gene position: 1030967-1030272 (Counterclockwise)

Preceding gene: 190571359

Following gene: 190571357

Centisome position: 69.54

GC content: 34.48

Gene sequence:

>696_bases
ATGAAAAAAATTAGGCTTTATGTTGAAGAAGCTTTATCACAAAATGTAAGTTTGGCGCTTCATCCACGACAAAGTCACTA
TATTTACAATGTAATGCGGCTTAAGAAGCATGATAACATCTATCTTTTTAATGGAAAGGATGGAGAATGGTTAGGAGAAG
TAGTGAATATATCAAGTAAATCGACAAAAATTATAATAAAAGAACGCACTAAACAACAACAACATGAAAAAAATCTATAT
TTGTATTGTGCTCTGGTAAAAAGCGGCGCCTTAAGCAACATAGTAAGACAAGCAACTGAAATGGGAGTAACCTGCATTCA
ATTTATTTCAACAGAACGTACAGTAGTAAAAAACATTAACCTAAGTAGAGCGAAATTACAGGCAATTGAAGCTGCGGAAC
AATCCGGTAGGACAAGTATACCACAGATTTTTCTTCCTATTAATTTTTGTGAATTATCCGATTCCCAGAATAAAAATTTT
GTTTTGTGTGATGAAACAGGTGAAGGACAACCTCCTAATAAAGTTCTAAAAGGCAAGAAAAACGTTGCTATTATTGTTGG
CCCCGAAGGTGGTTTTTCATCTTGCGAACTTGATTCTGCCAATAAATTTTGTCAGAAACTGAGTCTAGGAAAAAGAATTT
TGAGAGTTGATACTGCTGTAGTTGCTGCATTAACCTTCACCAATTGGTATAGCTAG

Upstream 100 bases:

>100_bases
CTTTCGTCTAGTGGTTAGGACACCACCCTTTCACGGTGGTAACACGGGTTCAAATCCCGTAAGAGTCACTCAAACAGAAT
GCAAATAACTAAAGTTTTGA

Downstream 100 bases:

>100_bases
ATTTTTTAATATGGCTAGTGCAACTTGACACTCTATATAAAGAAATCTGCATTATATTTTTCCTAAAAGAAATAAATGAA
ACTTGCATTTTTCCGAACTA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKKIRLYVEEALSQNVSLALHPRQSHYIYNVMRLKKHDNIYLFNGKDGEWLGEVVNISSKSTKIIIKERTKQQQHEKNLY
LYCALVKSGALSNIVRQATEMGVTCIQFISTERTVVKNINLSRAKLQAIEAAEQSGRTSIPQIFLPINFCELSDSQNKNF
VLCDETGEGQPPNKVLKGKKNVAIIVGPEGGFSSCELDSANKFCQKLSLGKRILRVDTAVVAALTFTNWYS

Sequences:

>Translated_231_residues
MKKIRLYVEEALSQNVSLALHPRQSHYIYNVMRLKKHDNIYLFNGKDGEWLGEVVNISSKSTKIIIKERTKQQQHEKNLY
LYCALVKSGALSNIVRQATEMGVTCIQFISTERTVVKNINLSRAKLQAIEAAEQSGRTSIPQIFLPINFCELSDSQNKNF
VLCDETGEGQPPNKVLKGKKNVAIIVGPEGGFSSCELDSANKFCQKLSLGKRILRVDTAVVAALTFTNWYS
>Mature_231_residues
MKKIRLYVEEALSQNVSLALHPRQSHYIYNVMRLKKHDNIYLFNGKDGEWLGEVVNISSKSTKIIIKERTKQQQHEKNLY
LYCALVKSGALSNIVRQATEMGVTCIQFISTERTVVKNINLSRAKLQAIEAAEQSGRTSIPQIFLPINFCELSDSQNKNF
VLCDETGEGQPPNKVLKGKKNVAIIVGPEGGFSSCELDSANKFCQKLSLGKRILRVDTAVVAALTFTNWYS

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=233, Percent_Identity=24.0343347639485, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25941; Mature: 25941

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIRLYVEEALSQNVSLALHPRQSHYIYNVMRLKKHDNIYLFNGKDGEWLGEVVNISSK
CCHHHHHHHHHHCCCCEEEECCCCCHHHHHEEEEECCCCEEEEECCCCHHHHHHHCCCCC
STKIIIKERTKQQQHEKNLYLYCALVKSGALSNIVRQATEMGVTCIQFISTERTVVKNIN
CEEEEEEHHHHHHHHHCCEEEEEEEHHCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHCCC
LSRAKLQAIEAAEQSGRTSIPQIFLPINFCELSDSQNKNFVLCDETGEGQPPNKVLKGKK
CCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHCCCC
NVAIIVGPEGGFSSCELDSANKFCQKLSLGKRILRVDTAVVAALTFTNWYS
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKIRLYVEEALSQNVSLALHPRQSHYIYNVMRLKKHDNIYLFNGKDGEWLGEVVNISSK
CCHHHHHHHHHHCCCCEEEECCCCCHHHHHEEEEECCCCEEEEECCCCHHHHHHHCCCCC
STKIIIKERTKQQQHEKNLYLYCALVKSGALSNIVRQATEMGVTCIQFISTERTVVKNIN
CEEEEEEHHHHHHHHHCCEEEEEEEHHCCHHHHHHHHHHHHCHHHHHHHHCCHHHHHCCC
LSRAKLQAIEAAEQSGRTSIPQIFLPINFCELSDSQNKNFVLCDETGEGQPPNKVLKGKK
CCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHCCCC
NVAIIVGPEGGFSSCELDSANKFCQKLSLGKRILRVDTAVVAALTFTNWYS
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]