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The map label for this gene is purF [H]

Identifier: 190571313

GI number: 190571313

Start: 987529

End: 988914

Strand: Reverse

Name: purF [H]

Synonym: WPa_0918

Alternate gene names: 190571313

Gene position: 988914-987529 (Counterclockwise)

Preceding gene: 190571316

Following gene: 190571310

Centisome position: 66.71

GC content: 36.08

Gene sequence:

>1386_bases
ATGCTTGATGAAGTACATGAGGAGTGTGGGGTATTTGGCATAAGTTGCAACCAAAGTGCTGCTTTTAACTCTATACTTGC
TCTCCACGCTTTGCAGCATAGAGGTCAAGAGTCTTTTGGTGTAGTAACCAGTAACAACGATAAGCTGCTCTCTTATCACT
TTCAAGGTCAAGTGAGCAGCGTATTTGATGATATAGATGAAATAAAGAAATCCTTACCTGGAGATTGTGCAATAGGTCAT
GTTCGATACTCAACCAGTGGTAGTAAATTTGGAGTGCAGCCCATGTTTGGCAAAAGTGGCAAATTTGGAGATTTCGCCAT
AGCACATAATGGTAATTTAATTAATATTTCTCCGATACGCGAACAATTAATTAAACAAGAGTGTGTTTTTCAGTCAGATA
TTGATACAGAAGTAGTAGTGCATCTGACAGCAAGCGGTGAAAAAGATAGCTTTTTAGAGAGTTTTATATATGCATTAAAG
CAAATACAAGGTGCTTATTCTTTTGTGGCAATCAATCAAGAAGTAGTTATTGGAGTACGTGATCCATCTGGAATCAGGCC
TCTTGTTTTAGGAAAGTTAAACGGTTCTTACGTTCTTGCATCTGAAACTTGTGCTCTTGATATAATAAATGCAGAGTTTA
TTAGAGAAATAGAACCAGGCGAATTAGTTACCATCAGTTCGGATAGTAAGCTAGCCTCAATGTTTCCTTTCCCAAAGCAA
AAATCAAGTTTTTGTATTTTTGAATACGTTTATTTTTCAAGACCAGACAGCATAATGGAGAATAGGTCTATATACGATAC
AAGAAAAGAAATAGGAAGAATACTTGCAAAGGAAAGTCCACCAAAGAACAATGTAGATATGATTGTACCAATACCTGATT
CTGGAATACCTGCAGCTATTGGATATGCAAAGCATTCAGGATTACCTATGGAACTGGGGATAATTCGTAATCATTATATA
GGAAGAACATTTATACAGCCAATCCCTGAAATACGTAAGGTTAGAATAAAATTAAAGTTCAATGCTAATAAGCATATTTT
AAAGGGTAAAAACATAATTTTAATAGACGATAGTATAGTGCGTGGTAGCACGCTCAGAAGTATAATAGTTATGTTAAAAG
ATGCAGGAGTAAAAGAAATTCACCTAAAGATTTCATGTCCACCAATTAAGCACTCTTGTTTTTATGGAATAGATACGCCA
GAGTGCAAAGATTTAATTGCTGCAAATAAATCTGTAGAGGAAATTAAGGAAGTTATAGGAATAGATAGCCTAGCCTTCTT
GAGTATTGATGGCCTATACAGGGCTGTTAAAGGAGAGGTGCGTAACAATGCTACACCGCAATATTGTGATGCTTGCTTCA
CTGGTGATTACCCAATTGGTAAGTAA

Upstream 100 bases:

>100_bases
AAACTACCGTAGAAAGCTTCAACTTTGAAAGGGATTAACAAATAAAATTCTTGTACTTTACCTCTACTAAAGTACAATCA
GGCTTTATTTAATATTTTTC

Downstream 100 bases:

>100_bases
TCTATTATTAATGAAGGTGAGGTTTTTTCTATGTTATATTAACAGTTTTAAAAATAGATTTTTGCATAACCATATAGTTT
TTGCCTGGGCGTCATTTGAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLDEVHEECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLLSYHFQGQVSSVFDDIDEIKKSLPGDCAIGH
VRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIREQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALK
QIQGAYSFVAINQEVVIGVRDPSGIRPLVLGKLNGSYVLASETCALDIINAEFIREIEPGELVTISSDSKLASMFPFPKQ
KSSFCIFEYVYFSRPDSIMENRSIYDTRKEIGRILAKESPPKNNVDMIVPIPDSGIPAAIGYAKHSGLPMELGIIRNHYI
GRTFIQPIPEIRKVRIKLKFNANKHILKGKNIILIDDSIVRGSTLRSIIVMLKDAGVKEIHLKISCPPIKHSCFYGIDTP
ECKDLIAANKSVEEIKEVIGIDSLAFLSIDGLYRAVKGEVRNNATPQYCDACFTGDYPIGK

Sequences:

>Translated_461_residues
MLDEVHEECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLLSYHFQGQVSSVFDDIDEIKKSLPGDCAIGH
VRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIREQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALK
QIQGAYSFVAINQEVVIGVRDPSGIRPLVLGKLNGSYVLASETCALDIINAEFIREIEPGELVTISSDSKLASMFPFPKQ
KSSFCIFEYVYFSRPDSIMENRSIYDTRKEIGRILAKESPPKNNVDMIVPIPDSGIPAAIGYAKHSGLPMELGIIRNHYI
GRTFIQPIPEIRKVRIKLKFNANKHILKGKNIILIDDSIVRGSTLRSIIVMLKDAGVKEIHLKISCPPIKHSCFYGIDTP
ECKDLIAANKSVEEIKEVIGIDSLAFLSIDGLYRAVKGEVRNNATPQYCDACFTGDYPIGK
>Mature_461_residues
MLDEVHEECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLLSYHFQGQVSSVFDDIDEIKKSLPGDCAIGH
VRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIREQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALK
QIQGAYSFVAINQEVVIGVRDPSGIRPLVLGKLNGSYVLASETCALDIINAEFIREIEPGELVTISSDSKLASMFPFPKQ
KSSFCIFEYVYFSRPDSIMENRSIYDTRKEIGRILAKESPPKNNVDMIVPIPDSGIPAAIGYAKHSGLPMELGIIRNHYI
GRTFIQPIPEIRKVRIKLKFNANKHILKGKNIILIDDSIVRGSTLRSIIVMLKDAGVKEIHLKISCPPIKHSCFYGIDTP
ECKDLIAANKSVEEIKEVIGIDSLAFLSIDGLYRAVKGEVRNNATPQYCDACFTGDYPIGK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=517, Percent_Identity=34.6228239845261, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1788651, Length=471, Percent_Identity=35.4564755838641, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1790167, Length=229, Percent_Identity=27.9475982532751, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=37.7083333333333, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=31.6770186335404, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=37.7551020408163, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=37.9828326180258, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI28573187, Length=499, Percent_Identity=36.2725450901804, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50887; Mature: 50887

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDEVHEECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLLSYHFQGQVSS
CCCHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCHHH
VFDDIDEIKKSLPGDCAIGHVRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIR
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHH
EQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALKQIQGAYSFVAINQEVVIGVR
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEC
DPSGIRPLVLGKLNGSYVLASETCALDIINAEFIREIEPGELVTISSDSKLASMFPFPKQ
CCCCCCCEEEEECCCCEEEEECCEEEEEECHHHHHCCCCCCEEEEECCCCHHHHCCCCCC
KSSFCIFEYVYFSRPDSIMENRSIYDTRKEIGRILAKESPPKNNVDMIVPIPDSGIPAAI
CCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHE
GYAKHSGLPMELGIIRNHYIGRTFIQPIPEIRKVRIKLKFNANKHILKGKNIILIDDSIV
EHHHCCCCCEEEHHHHHCCCCHHHHHCCCCEEEEEEEEEECCCCEEECCCCEEEECCCCC
RGSTLRSIIVMLKDAGVKEIHLKISCPPIKHSCFYGIDTPECKDLIAANKSVEEIKEVIG
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHC
IDSLAFLSIDGLYRAVKGEVRNNATPQYCDACFTGDYPIGK
CCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHEECCCCCCCC
>Mature Secondary Structure
MLDEVHEECGVFGISCNQSAAFNSILALHALQHRGQESFGVVTSNNDKLLSYHFQGQVSS
CCCHHHHHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCHHH
VFDDIDEIKKSLPGDCAIGHVRYSTSGSKFGVQPMFGKSGKFGDFAIAHNGNLINISPIR
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEHHHH
EQLIKQECVFQSDIDTEVVVHLTASGEKDSFLESFIYALKQIQGAYSFVAINQEVVIGVR
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEC
DPSGIRPLVLGKLNGSYVLASETCALDIINAEFIREIEPGELVTISSDSKLASMFPFPKQ
CCCCCCCEEEEECCCCEEEEECCEEEEEECHHHHHCCCCCCEEEEECCCCHHHHCCCCCC
KSSFCIFEYVYFSRPDSIMENRSIYDTRKEIGRILAKESPPKNNVDMIVPIPDSGIPAAI
CCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHE
GYAKHSGLPMELGIIRNHYIGRTFIQPIPEIRKVRIKLKFNANKHILKGKNIILIDDSIV
EHHHCCCCCEEEHHHHHCCCCHHHHHCCCCEEEEEEEEEECCCCEEECCCCEEEECCCCC
RGSTLRSIIVMLKDAGVKEIHLKISCPPIKHSCFYGIDTPECKDLIAANKSVEEIKEVIG
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHC
IDSLAFLSIDGLYRAVKGEVRNNATPQYCDACFTGDYPIGK
CCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]