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The map label for this gene is kefB [H]

Identifier: 190571300

GI number: 190571300

Start: 970009

End: 971727

Strand: Reverse

Name: kefB [H]

Synonym: WPa_0903

Alternate gene names: 190571300

Gene position: 971727-970009 (Counterclockwise)

Preceding gene: 190571301

Following gene: 190571299

Centisome position: 65.55

GC content: 36.3

Gene sequence:

>1719_bases
ATGCACAGCGGCTCTCAGCATTTGTTTGATATTATAATCTTACTTTCCGCTGCTGTGTTTATAGTCATAGCGTTTTGGAA
AATGAATATTAGTCCAGTGCTTGGTTACTTTGTTGCAGGTGCATTGATTGGTTCTCATGGATTTAATCTGATACATTCAG
TTGAAGTAATGGATAACTTTGCAGAGTTTGGCGTAGTTTTCCTATTGTTCATTATAGGTCTTGAATTGACATTTGAACGA
CTTATCGCTATGCGTAGGCATGTGTTTGGGTTTGGTTCCCTTCAAGTTATAGTTACCATGATAGCAATATGGTGCATTGC
TCTTGCCTTTGGAGTAAATACAAACATGGCAGCAGTTATTGGTGGTGGACTTGCACTATCCTCAACAGCAATAGTGTTGC
AGGTTCTGCAAGAAAAGGGTTCTCAAGCAAGCCAAGTTGGTAGATTGTCAATAGCAGTGCTACTAATGCAGGACTTTGCA
GTGGTACCTCTAATAGTACTAGTGCCCCTGCTTACTGGCAATTCTGAACACAGCTTAATAAGCTCGTTGGCAGGCTCATT
AGTGCAAGCAGCTATTGCATTGGTGTTGATATTTGTAACTGGTAGGTTATTACTGAGGCCATTGTTTTCGGTTATTGCCA
AAATGGAGAGTAATGAAATCTTTATATCAACAACACTTCTGATAATATTAGGATCAGCATTTATTACTGAACAATTTCAT
TTATCAATGGCATTAGGCGCATTTGTTGCTGGGTTACTAGTTGCAGAAACAGAACACAGGAATTCGGTAGAACATGCAGT
ATTGCCATTTAAAGACTTATTTCTTGGTTTATTCTTTATGACTGTTGGCATGTCTATTGATATTGACCTTTTACTCAATA
AGTTACCACTCGTTACTTTATTATCAATTATCCTTATCGTTTTAAAGACGTCCATTATATATACATTGTGTAGATTTTTT
GGATTTAGAAGTGCACCTGCAATACAAGCTGGATTACTGCTTGCACAAGGCGGTGAATTTGCATTTATTTTATTCCGTTT
AGCAGATGAATTAGATGTACTATCAAGTGAAATCGCTCAAGTGCTTATGATGGTAACTACAGTAACTATGGCTTTTACTC
CTCTTTTATCGGGAATTGGAGACTGGATAGCAAATTCATTTAGCACTGAAAAAATAATACTAGACGATGAAGCAATCGAA
ACAGACACACAAGATCTTTATAACCACGTAATAGTTGCCGGGTTTGGTAGAGTGGGATATATGATAACGAAAATGCTTAC
TGCAGAGCATTTAAGTTATGTTGTTGTAGATATTCAATCAAAAATAGTGAAAGAAGGAAAAAATGACAGTTTCCCTATAT
ATCTTGGAGATGCTACAAGATATGAAATTTTAAAATCAGTAGGAATAGAAAGAGCTCAAGCTCTTGTGGTTTCAATAAAG
AACGAAGTCACTATAAAGAAAATAGTTTCTCTAGTTGCAACAAACTTCCCACATGTAAATATTATAATACGTTTACCAGA
TTTGAATAATGTGGAGGTTTATAAAGATCTAGGGGCTAGTAAAATTATTCCCGAGACGTCTGAGATTGGATTGCAGCTAG
GTGGAGCAGCATTAAGCTTAAGTGGAATTAGCGAAAGCGGAGTTACATCTTTAAAAGGTAAATTTAGAAAAGGCAACTAC
AGTATGTTAAAAGACCTTGGTAGTGATAAAGATGAATAA

Upstream 100 bases:

>100_bases
AAGCGATGAAATTTATTGCAAAAACTAGCAATCTTCCAATTGACCAAAAATCAAGTATTGGTTGTTCAATAAAATGGTCT
AATTCTACAGGTTAAATATT

Downstream 100 bases:

>100_bases
TCCTCTATCACTTTAATTAGTGATCAGAAAAACACATTTAATTAAGCAACTATACTCTCTAGAAAGGTGAAATTGACCTT
ATTGTAACTATGAACAAAAT

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MHSGSQHLFDIIILLSAAVFIVIAFWKMNISPVLGYFVAGALIGSHGFNLIHSVEVMDNFAEFGVVFLLFIIGLELTFER
LIAMRRHVFGFGSLQVIVTMIAIWCIALAFGVNTNMAAVIGGGLALSSTAIVLQVLQEKGSQASQVGRLSIAVLLMQDFA
VVPLIVLVPLLTGNSEHSLISSLAGSLVQAAIALVLIFVTGRLLLRPLFSVIAKMESNEIFISTTLLIILGSAFITEQFH
LSMALGAFVAGLLVAETEHRNSVEHAVLPFKDLFLGLFFMTVGMSIDIDLLLNKLPLVTLLSIILIVLKTSIIYTLCRFF
GFRSAPAIQAGLLLAQGGEFAFILFRLADELDVLSSEIAQVLMMVTTVTMAFTPLLSGIGDWIANSFSTEKIILDDEAIE
TDTQDLYNHVIVAGFGRVGYMITKMLTAEHLSYVVVDIQSKIVKEGKNDSFPIYLGDATRYEILKSVGIERAQALVVSIK
NEVTIKKIVSLVATNFPHVNIIIRLPDLNNVEVYKDLGASKIIPETSEIGLQLGGAALSLSGISESGVTSLKGKFRKGNY
SMLKDLGSDKDE

Sequences:

>Translated_572_residues
MHSGSQHLFDIIILLSAAVFIVIAFWKMNISPVLGYFVAGALIGSHGFNLIHSVEVMDNFAEFGVVFLLFIIGLELTFER
LIAMRRHVFGFGSLQVIVTMIAIWCIALAFGVNTNMAAVIGGGLALSSTAIVLQVLQEKGSQASQVGRLSIAVLLMQDFA
VVPLIVLVPLLTGNSEHSLISSLAGSLVQAAIALVLIFVTGRLLLRPLFSVIAKMESNEIFISTTLLIILGSAFITEQFH
LSMALGAFVAGLLVAETEHRNSVEHAVLPFKDLFLGLFFMTVGMSIDIDLLLNKLPLVTLLSIILIVLKTSIIYTLCRFF
GFRSAPAIQAGLLLAQGGEFAFILFRLADELDVLSSEIAQVLMMVTTVTMAFTPLLSGIGDWIANSFSTEKIILDDEAIE
TDTQDLYNHVIVAGFGRVGYMITKMLTAEHLSYVVVDIQSKIVKEGKNDSFPIYLGDATRYEILKSVGIERAQALVVSIK
NEVTIKKIVSLVATNFPHVNIIIRLPDLNNVEVYKDLGASKIIPETSEIGLQLGGAALSLSGISESGVTSLKGKFRKGNY
SMLKDLGSDKDE
>Mature_572_residues
MHSGSQHLFDIIILLSAAVFIVIAFWKMNISPVLGYFVAGALIGSHGFNLIHSVEVMDNFAEFGVVFLLFIIGLELTFER
LIAMRRHVFGFGSLQVIVTMIAIWCIALAFGVNTNMAAVIGGGLALSSTAIVLQVLQEKGSQASQVGRLSIAVLLMQDFA
VVPLIVLVPLLTGNSEHSLISSLAGSLVQAAIALVLIFVTGRLLLRPLFSVIAKMESNEIFISTTLLIILGSAFITEQFH
LSMALGAFVAGLLVAETEHRNSVEHAVLPFKDLFLGLFFMTVGMSIDIDLLLNKLPLVTLLSIILIVLKTSIIYTLCRFF
GFRSAPAIQAGLLLAQGGEFAFILFRLADELDVLSSEIAQVLMMVTTVTMAFTPLLSGIGDWIANSFSTEKIILDDEAIE
TDTQDLYNHVIVAGFGRVGYMITKMLTAEHLSYVVVDIQSKIVKEGKNDSFPIYLGDATRYEILKSVGIERAQALVVSIK
NEVTIKKIVSLVATNFPHVNIIIRLPDLNNVEVYKDLGASKIIPETSEIGLQLGGAALSLSGISESGVTSLKGKFRKGNY
SMLKDLGSDKDE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=380, Percent_Identity=27.8947368421053, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1786232, Length=565, Percent_Identity=36.283185840708, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1789749, Length=568, Percent_Identity=36.7957746478873, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1786685, Length=514, Percent_Identity=29.7665369649805, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 61988; Mature: 61988

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCHHHCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure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CCCCCCCCHHHCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA