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The map label for this gene is folA [H]

Identifier: 190571098

GI number: 190571098

Start: 765369

End: 765848

Strand: Reverse

Name: folA [H]

Synonym: WPa_0694

Alternate gene names: 190571098

Gene position: 765848-765369 (Counterclockwise)

Preceding gene: 190571099

Following gene: 190571095

Centisome position: 51.66

GC content: 35.0

Gene sequence:

>480_bases
ATGAAGATTATTGGAATTATGGCTGTTGACCCTAATGGGGTAATTGGAATAAATAATGATTTGCCCTGGCGTTATCCAAG
TGAGTTTAAACATTTTTGTCAAGTAACTGACAAGCAAGTTATTGTGATGGGAAGAAAGACATTTGAAACCGTGCCTCAGA
GTATACTAAAGAATCGTACACCTATTGTTTTCTCCCGTAACAAGTGTTTTAATAGAGGCCCAAAATGTACTGTTGTCTCT
TCCATGCAAGAATTTCTGTCAATTCAAAGTAGTTCTCAAGTCTTTGTGATTGGTGGGGCACAAATAGTACACCTTTTTTT
AGAGCATAACCTAATCTCAGAATTTATTATGACTGAAATGCATAAAACTTATAAAGGTGATACGTATTTCAATTTAGCAC
TCCTTGATCAGTGGAATAAAACTATTCTCACTAAAACTAAAGACTATACTATATGCAATTGTGTCCGTTCAGCAGAGTAG

Upstream 100 bases:

>100_bases
TTGCTATATCTGCTATACTGCAAAATAAGGTTGATTTCCTTAGAGTGCATAATGTACGCGACCACATGAGATTTTTTGTA
GCCCAAGCTGTCTTAAAGTG

Downstream 100 bases:

>100_bases
TAAAATAAGGTAGGCGAGAAAAGACAACTATCAGTGCATGACACTGGCTCCTTTATGATGGTCAGTGCCCAGAAACTGGC
ATCCAGTTAAATTTATGGAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKIIGIMAVDPNGVIGINNDLPWRYPSEFKHFCQVTDKQVIVMGRKTFETVPQSILKNRTPIVFSRNKCFNRGPKCTVVS
SMQEFLSIQSSSQVFVIGGAQIVHLFLEHNLISEFIMTEMHKTYKGDTYFNLALLDQWNKTILTKTKDYTICNCVRSAE

Sequences:

>Translated_159_residues
MKIIGIMAVDPNGVIGINNDLPWRYPSEFKHFCQVTDKQVIVMGRKTFETVPQSILKNRTPIVFSRNKCFNRGPKCTVVS
SMQEFLSIQSSSQVFVIGGAQIVHLFLEHNLISEFIMTEMHKTYKGDTYFNLALLDQWNKTILTKTKDYTICNCVRSAE
>Mature_159_residues
MKIIGIMAVDPNGVIGINNDLPWRYPSEFKHFCQVTDKQVIVMGRKTFETVPQSILKNRTPIVFSRNKCFNRGPKCTVVS
SMQEFLSIQSSSQVFVIGGAQIVHLFLEHNLISEFIMTEMHKTYKGDTYFNLALLDQWNKTILTKTKDYTICNCVRSAE

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17505899, Length=141, Percent_Identity=30.4964539007092, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324810, Length=160, Percent_Identity=29.375, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18214; Mature: 18214

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIGIMAVDPNGVIGINNDLPWRYPSEFKHFCQVTDKQVIVMGRKTFETVPQSILKNRT
CEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCC
PIVFSRNKCFNRGPKCTVVSSMQEFLSIQSSSQVFVIGGAQIVHLFLEHNLISEFIMTEM
CEEEECCCCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
HKTYKGDTYFNLALLDQWNKTILTKTKDYTICNCVRSAE
HHHHCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MKIIGIMAVDPNGVIGINNDLPWRYPSEFKHFCQVTDKQVIVMGRKTFETVPQSILKNRT
CEEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCC
PIVFSRNKCFNRGPKCTVVSSMQEFLSIQSSSQVFVIGGAQIVHLFLEHNLISEFIMTEM
CEEEECCCCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHH
HKTYKGDTYFNLALLDQWNKTILTKTKDYTICNCVRSAE
HHHHCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7574597; 11337471 [H]