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The map label for this gene is dapE

Identifier: 190571095

GI number: 190571095

Start: 761165

End: 762361

Strand: Reverse

Name: dapE

Synonym: WPa_0691

Alternate gene names: 190571095

Gene position: 762361-761165 (Counterclockwise)

Preceding gene: 190571098

Following gene: 190571094

Centisome position: 51.43

GC content: 34.59

Gene sequence:

>1197_bases
ATGAAGATTGACCCTGTAGAACTAACTAAGAAATTAATTTCTTTTAAAAGTATAACACCAAAAGACGATGGGGCAATAGA
GCATATAGCAGCCATTCTCAAGAAAAGTGGTTTTGAATGTGAGATTTTAGAGTTTGGTGATAAAGTTAAAAATCTTTATG
CGAAATATATAAATGGAGTACCAAACTTGTGTTTTGCTGGACATGTTGATGTTGTACCACCAGGTGAGTTAAAAGATTGG
ATATCTGATCCATTTAAGCCAGAAATTAGAGATGGAATGCTATATGGAAGAGGAGCAGCTGATATGAAAAGTGGAATAGC
CGCATTTATTGCTGCTATCGTAGATTCAGTTGCAGGTAAGTTTCGATTCAGCGGTTCAATCAGTGCATTGATTACCAGCG
CTGAAGAAAGCACGGAAGAACATGGAACAAAGGCCGTTTTGGAATGGATGAAAAGCAAGCAAAAAAAGATAGATTTTTGT
ATAGTTGGAGAACCAACGAGTAGTGAGAAATTAGGTGATACCATAAAAATAGGTAGAAGAGGTTCTGCAACATTTAAATT
GATTTGCCATGGAAAACAAGGGCACGTTGCCTATCCAGAACTAGCGGATAATCCAATATATAAAATGATATCGATACTAA
GTAAGATAAAAGATACCACTTTTGATACTGGTAATAAATATTTTCAGCCTTCACATTGCGAAATTACTACTATCGACGTT
GGAAATAATACTAATAATCTAATACCTAGCTCAATAGCAGCAGGTTTCAATATTCGATATAACAACATACAGACGCTTGA
CGGCTTATATAAGCTGATTGATGAAATATGCTCTAGTGTAACTAACGATTATAAACTGTCTATGCAGAGCAGCAGAAACG
TTTTTCTCTCTATTCCTGATAGAAATACTGATATTATGCTTGATGCGATAAATAAAATTACCGGCATTGATGCTGTGCTG
AGTACAAATGGTGGCACATCTGATGCTGCGTTTATTAAGGATATTTGTCCAGTAATTGAATTTGGTATGATCAACAAAAC
CGCACATCAGGTAAATGAATGCGTATCAATAGACGATATACATAAATTAACAGCTATATATAAGGAGTTTATAAAAAATT
ATTTTTACCCCACTAATAAAATATTAAACCAGATCAATGTAATTGGTAATACGCCTGATGGTCCATTATTAGCTTGA

Upstream 100 bases:

>100_bases
CAACTTCCCTAATAAGAACTTGTTCTGTGTGCTGTAATATTTGTTTTAAATATCGCATAGAATGTTTACAATACGGTAAT
GTTAGTATTTCAAGATTGGT

Downstream 100 bases:

>100_bases
GGTGCAGTTGCATAGAGAAGAAAACTTACTAGACACATTTTACCAGCCCCTTATTATAATATTAAGGGTATTTTTGACTC
AAAACTTGTCTTTGATCTGT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKIDPVELTKKLISFKSITPKDDGAIEHIAAILKKSGFECEILEFGDKVKNLYAKYINGVPNLCFAGHVDVVPPGELKDW
ISDPFKPEIRDGMLYGRGAADMKSGIAAFIAAIVDSVAGKFRFSGSISALITSAEESTEEHGTKAVLEWMKSKQKKIDFC
IVGEPTSSEKLGDTIKIGRRGSATFKLICHGKQGHVAYPELADNPIYKMISILSKIKDTTFDTGNKYFQPSHCEITTIDV
GNNTNNLIPSSIAAGFNIRYNNIQTLDGLYKLIDEICSSVTNDYKLSMQSSRNVFLSIPDRNTDIMLDAINKITGIDAVL
STNGGTSDAAFIKDICPVIEFGMINKTAHQVNECVSIDDIHKLTAIYKEFIKNYFYPTNKILNQINVIGNTPDGPLLA

Sequences:

>Translated_398_residues
MKIDPVELTKKLISFKSITPKDDGAIEHIAAILKKSGFECEILEFGDKVKNLYAKYINGVPNLCFAGHVDVVPPGELKDW
ISDPFKPEIRDGMLYGRGAADMKSGIAAFIAAIVDSVAGKFRFSGSISALITSAEESTEEHGTKAVLEWMKSKQKKIDFC
IVGEPTSSEKLGDTIKIGRRGSATFKLICHGKQGHVAYPELADNPIYKMISILSKIKDTTFDTGNKYFQPSHCEITTIDV
GNNTNNLIPSSIAAGFNIRYNNIQTLDGLYKLIDEICSSVTNDYKLSMQSSRNVFLSIPDRNTDIMLDAINKITGIDAVL
STNGGTSDAAFIKDICPVIEFGMINKTAHQVNECVSIDDIHKLTAIYKEFIKNYFYPTNKILNQINVIGNTPDGPLLA
>Mature_398_residues
MKIDPVELTKKLISFKSITPKDDGAIEHIAAILKKSGFECEILEFGDKVKNLYAKYINGVPNLCFAGHVDVVPPGELKDW
ISDPFKPEIRDGMLYGRGAADMKSGIAAFIAAIVDSVAGKFRFSGSISALITSAEESTEEHGTKAVLEWMKSKQKKIDFC
IVGEPTSSEKLGDTIKIGRRGSATFKLICHGKQGHVAYPELADNPIYKMISILSKIKDTTFDTGNKYFQPSHCEITTIDV
GNNTNNLIPSSIAAGFNIRYNNIQTLDGLYKLIDEICSSVTNDYKLSMQSSRNVFLSIPDRNTDIMLDAINKITGIDAVL
STNGGTSDAAFIKDICPVIEFGMINKTAHQVNECVSIDDIHKLTAIYKEFIKNYFYPTNKILNQINVIGNTPDGPLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=379, Percent_Identity=38.7862796833773, Blast_Score=274, Evalue=8e-75,
Organism=Escherichia coli, GI1790395, Length=394, Percent_Identity=24.3654822335025, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789236, Length=281, Percent_Identity=22.4199288256228, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_WOLPP (B3CLN1)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975453.1
- ProteinModelPortal:   B3CLN1
- MEROPS:   M20.010
- EnsemblBacteria:   EBWOLT00000001055
- GeneID:   6384940
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0691
- GeneTree:   EBGT00050000031274
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 43708; Mature: 43708

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 70-70 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDPVELTKKLISFKSITPKDDGAIEHIAAILKKSGFECEILEFGDKVKNLYAKYINGV
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCC
PNLCFAGHVDVVPPGELKDWISDPFKPEIRDGMLYGRGAADMKSGIAAFIAAIVDSVAGK
CCCEECCCCCCCCCCHHHHHHCCCCCCCHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
FRFSGSISALITSAEESTEEHGTKAVLEWMKSKQKKIDFCIVGEPTSSEKLGDTIKIGRR
EEECCCHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCHHEECCC
GSATFKLICHGKQGHVAYPELADNPIYKMISILSKIKDTTFDTGNKYFQPSHCEITTIDV
CCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
GNNTNNLIPSSIAAGFNIRYNNIQTLDGLYKLIDEICSSVTNDYKLSMQSSRNVFLSIPD
CCCCCCCCCHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCC
RNTDIMLDAINKITGIDAVLSTNGGTSDAAFIKDICPVIEFGMINKTAHQVNECVSIDDI
CCCCHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
HKLTAIYKEFIKNYFYPTNKILNQINVIGNTPDGPLLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIDPVELTKKLISFKSITPKDDGAIEHIAAILKKSGFECEILEFGDKVKNLYAKYINGV
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCC
PNLCFAGHVDVVPPGELKDWISDPFKPEIRDGMLYGRGAADMKSGIAAFIAAIVDSVAGK
CCCEECCCCCCCCCCHHHHHHCCCCCCCHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
FRFSGSISALITSAEESTEEHGTKAVLEWMKSKQKKIDFCIVGEPTSSEKLGDTIKIGRR
EEECCCHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCHHEECCC
GSATFKLICHGKQGHVAYPELADNPIYKMISILSKIKDTTFDTGNKYFQPSHCEITTIDV
CCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
GNNTNNLIPSSIAAGFNIRYNNIQTLDGLYKLIDEICSSVTNDYKLSMQSSRNVFLSIPD
CCCCCCCCCHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCC
RNTDIMLDAINKITGIDAVLSTNGGTSDAAFIKDICPVIEFGMINKTAHQVNECVSIDDI
CCCCHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
HKLTAIYKEFIKNYFYPTNKILNQINVIGNTPDGPLLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA