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The map label for this gene is gidA

Identifier: 190571050

GI number: 190571050

Start: 704611

End: 706659

Strand: Reverse

Name: gidA

Synonym: WPa_0643

Alternate gene names: 190571050

Gene position: 706659-704611 (Counterclockwise)

Preceding gene: 190571051

Following gene: 190571049

Centisome position: 47.67

GC content: 38.6

Gene sequence:

>2049_bases
ATGCATAAGTACGACGTAGTGGTAGTAGGTGGCGGTCATGCCGGGTGTGAAGCAGCTACAGCTGCAGCACGTCTTGGTGC
AAGCACGCTACTTATAACTCACAAAATTTCAACTATAGGAGAGATGTCTTGTAATCCAGCAATTGGAGGAGTTGCAAAGG
GTGTTGTAGTTAGAGAAGTAGACGCCCTTGATGGAATAATGGGGAGAGCTATCGACCGAGCAAGCATACACTCGGTCGTT
TTAAATGGCAGCAGAGGCGCAGCAGTATGGGGCCCACGTGCACAAGCAGACCGAAAATTATACAAAAAAGCAATACAGGA
AATTATTCTAAATTATAACAACTTGACAGTAAAAGAAGAGTCAGTTGACGATTTTCTTATCGAAAGTGATAGCAATGGAG
AATCTTATATAAAAGCTGTAATAACAGACTCAGGTGAACGTATACTGACAAGCAGAGTTGTTTTGACTACAGGGACTTTT
TTACGTGGTGTGATTCATATAGGAGAGCAAACAACTCCTTCTGGAAGAATAGGAGATAAACCTGCAATAGAGCTTGCAAA
CACGCTAAAGAAGTATGATTTTAAACTGGGCAGGTTACGTACCGGAACTCCACCGAGGCTTGATCGTGGCACTATAAACT
GGTCGGTATTACAAGAACAAGTGGGTGACAATCCACCTACACCATTTTCTTATCTTACAGAGAAAATTAACCAGCCTCAG
GTTTCATGTTTTATTACTCATACTAATGAACATACACACAGAGTAATTCGAGAGAATCTTCACAGATCAGCTTCTTCATA
TTTAGATAACGTTATTGCACCAAGATACTGCCCATCAATTGAAACTAAAGTTAAAAAATTTGCAGAAAAAAATAATCACC
AAATATTTCTAGAACCAGAAGGGATTGATGATAACACTATATATCCAAATGGAATTTCAAATTCATTGCCCATCGAAGTG
CAGTATGAAATGATAAAGAGTATAAAAGGGCTTGAAAATGCAGAAATATTAAGACCTGGATATGCAGTTGAATACGATTA
TATCGATCCACGAGAGCTATTTCATACTCTCGAAACTAAGAAAGTTAAAGGACTATATTTTGCTGGTCAAATTAATGGTA
CCACTGGATATGAAGAAGCAGCTGGGCAGGGAATTATTGCCGGAATCAATGCAGCACTCTCTTTATCTCAAAAAAGTTTT
GTTCTTCATCGTACAGACTCATATATCGGTGTAATGATAGACGACTTGGTCACTAAGGGAATTACCGAGCCTTACAGATT
ATTTACCTCGCGTGCAGAATATAGACTGGCAATCAGGTCAGATAATGCAGATAGAAGATTGACACAAAAAGGTTATGACA
TCTCTCTTGTGTCGCATGAGAGATACTCTGTTTTGCAGGGTAAACTTAAATCCATTAAGCAACTTGAGGAGAAATTAGAG
AGCCTAACAATTACTCCTGAGCAGCTCAGGTCCTATGGCATTAAAATATCTTATGATGGAATAAGAAAAACAGCATTAGA
TTTGCTCGGTTATCCAAATATTGACTGGAATAAATTACGAGAAATATGGCCAGAGTTAAACATGGGTTCCAGTGTCAGCT
ACTTGCATAACGCCAAAGCACCTCTTCCTGTCATCCAAGTAGCTGACACTGGGATCCAGTACTTGAATGATGATGGAATG
GATGCAGTTGACACTGAAAAAAATGTAGATTCCAGCGTCATGTGCTGGAATAACACTATAACTGATAGCATAGCCAAGAA
TGAAATATATGAAGCAGTTGCAATTGAAGCAAAATACAAGCCTTATTTAGTAAGACAAGAAGCAGATATGAAGTTTCTAC
AAGAGGAAGTTAATACTCAAATTCCAACTAATTTCAACTACTCACAGATTAAAGGCTTGTCAACTGAAGTGATAGAAAAG
TTGCAGTCAATAAAGCCAGCAACAATTGGTATTGCGAAGCAAATACAAGGTATCACTCCAGCAGCGATAGTTAGCATATT
AGTGTACCTTAGGAATAAGAAAACGAAAATAGCTGCTAATTCTGCGTAA

Upstream 100 bases:

>100_bases
CGATAATTTGAGCTAAGTTAGTTTGTAACACTGGAATCTGTAAAATTTGACATCAATCACTGATAGTTGGAATATTATAG
ATTAGGAATTACTCAAGAAG

Downstream 100 bases:

>100_bases
CATGTCAAAAATCGAAGAATTTCGCTCTTTTATGCACGAAATAAATGTTGATACATTTATGTTGAATACTAAAGACGAGT
ATTTAAATGAATATTCAAAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MHKYDVVVVGGGHAGCEAATAAARLGASTLLITHKISTIGEMSCNPAIGGVAKGVVVREVDALDGIMGRAIDRASIHSVV
LNGSRGAAVWGPRAQADRKLYKKAIQEIILNYNNLTVKEESVDDFLIESDSNGESYIKAVITDSGERILTSRVVLTTGTF
LRGVIHIGEQTTPSGRIGDKPAIELANTLKKYDFKLGRLRTGTPPRLDRGTINWSVLQEQVGDNPPTPFSYLTEKINQPQ
VSCFITHTNEHTHRVIRENLHRSASSYLDNVIAPRYCPSIETKVKKFAEKNNHQIFLEPEGIDDNTIYPNGISNSLPIEV
QYEMIKSIKGLENAEILRPGYAVEYDYIDPRELFHTLETKKVKGLYFAGQINGTTGYEEAAGQGIIAGINAALSLSQKSF
VLHRTDSYIGVMIDDLVTKGITEPYRLFTSRAEYRLAIRSDNADRRLTQKGYDISLVSHERYSVLQGKLKSIKQLEEKLE
SLTITPEQLRSYGIKISYDGIRKTALDLLGYPNIDWNKLREIWPELNMGSSVSYLHNAKAPLPVIQVADTGIQYLNDDGM
DAVDTEKNVDSSVMCWNNTITDSIAKNEIYEAVAIEAKYKPYLVRQEADMKFLQEEVNTQIPTNFNYSQIKGLSTEVIEK
LQSIKPATIGIAKQIQGITPAAIVSILVYLRNKKTKIAANSA

Sequences:

>Translated_682_residues
MHKYDVVVVGGGHAGCEAATAAARLGASTLLITHKISTIGEMSCNPAIGGVAKGVVVREVDALDGIMGRAIDRASIHSVV
LNGSRGAAVWGPRAQADRKLYKKAIQEIILNYNNLTVKEESVDDFLIESDSNGESYIKAVITDSGERILTSRVVLTTGTF
LRGVIHIGEQTTPSGRIGDKPAIELANTLKKYDFKLGRLRTGTPPRLDRGTINWSVLQEQVGDNPPTPFSYLTEKINQPQ
VSCFITHTNEHTHRVIRENLHRSASSYLDNVIAPRYCPSIETKVKKFAEKNNHQIFLEPEGIDDNTIYPNGISNSLPIEV
QYEMIKSIKGLENAEILRPGYAVEYDYIDPRELFHTLETKKVKGLYFAGQINGTTGYEEAAGQGIIAGINAALSLSQKSF
VLHRTDSYIGVMIDDLVTKGITEPYRLFTSRAEYRLAIRSDNADRRLTQKGYDISLVSHERYSVLQGKLKSIKQLEEKLE
SLTITPEQLRSYGIKISYDGIRKTALDLLGYPNIDWNKLREIWPELNMGSSVSYLHNAKAPLPVIQVADTGIQYLNDDGM
DAVDTEKNVDSSVMCWNNTITDSIAKNEIYEAVAIEAKYKPYLVRQEADMKFLQEEVNTQIPTNFNYSQIKGLSTEVIEK
LQSIKPATIGIAKQIQGITPAAIVSILVYLRNKKTKIAANSA
>Mature_682_residues
MHKYDVVVVGGGHAGCEAATAAARLGASTLLITHKISTIGEMSCNPAIGGVAKGVVVREVDALDGIMGRAIDRASIHSVV
LNGSRGAAVWGPRAQADRKLYKKAIQEIILNYNNLTVKEESVDDFLIESDSNGESYIKAVITDSGERILTSRVVLTTGTF
LRGVIHIGEQTTPSGRIGDKPAIELANTLKKYDFKLGRLRTGTPPRLDRGTINWSVLQEQVGDNPPTPFSYLTEKINQPQ
VSCFITHTNEHTHRVIRENLHRSASSYLDNVIAPRYCPSIETKVKKFAEKNNHQIFLEPEGIDDNTIYPNGISNSLPIEV
QYEMIKSIKGLENAEILRPGYAVEYDYIDPRELFHTLETKKVKGLYFAGQINGTTGYEEAAGQGIIAGINAALSLSQKSF
VLHRTDSYIGVMIDDLVTKGITEPYRLFTSRAEYRLAIRSDNADRRLTQKGYDISLVSHERYSVLQGKLKSIKQLEEKLE
SLTITPEQLRSYGIKISYDGIRKTALDLLGYPNIDWNKLREIWPELNMGSSVSYLHNAKAPLPVIQVADTGIQYLNDDGM
DAVDTEKNVDSSVMCWNNTITDSIAKNEIYEAVAIEAKYKPYLVRQEADMKFLQEEVNTQIPTNFNYSQIKGLSTEVIEK
LQSIKPATIGIAKQIQGITPAAIVSILVYLRNKKTKIAANSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=688, Percent_Identity=44.3313953488372, Blast_Score=547, Evalue=1e-155,
Organism=Homo sapiens, GI19882217, Length=713, Percent_Identity=42.7769985974755, Blast_Score=533, Evalue=1e-151,
Organism=Homo sapiens, GI183227703, Length=728, Percent_Identity=41.8956043956044, Blast_Score=528, Evalue=1e-150,
Organism=Escherichia coli, GI2367273, Length=677, Percent_Identity=45.642540620384, Blast_Score=580, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI17534255, Length=676, Percent_Identity=39.792899408284, Blast_Score=472, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321202, Length=684, Percent_Identity=41.812865497076, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24658174, Length=693, Percent_Identity=44.011544011544, Blast_Score=540, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_WOLPP (B3CLI3)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975408.1
- ProteinModelPortal:   B3CLI3
- EnsemblBacteria:   EBWOLT00000000399
- GeneID:   6384500
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0643
- GeneTree:   EBGT00050000031046
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 75584; Mature: 75584

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKYDVVVVGGGHAGCEAATAAARLGASTLLITHKISTIGEMSCNPAIGGVAKGVVVREV
CCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCCCCCHHHCCHHEEHH
DALDGIMGRAIDRASIHSVVLNGSRGAAVWGPRAQADRKLYKKAIQEIILNYNNLTVKEE
HHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
SVDDFLIESDSNGESYIKAVITDSGERILTSRVVLTTGTFLRGVIHIGEQTTPSGRIGDK
CCCCEEEECCCCCHHEEEEEEECCCCEEEHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
PAIELANTLKKYDFKLGRLRTGTPPRLDRGTINWSVLQEQVGDNPPTPFSYLTEKINQPQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCC
VSCFITHTNEHTHRVIRENLHRSASSYLDNVIAPRYCPSIETKVKKFAEKNNHQIFLEPE
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECC
GIDDNTIYPNGISNSLPIEVQYEMIKSIKGLENAEILRPGYAVEYDYIDPRELFHTLETK
CCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCEECCCCEEEECCCCHHHHHHHHHHH
KVKGLYFAGQINGTTGYEEAAGQGIIAGINAALSLSQKSFVLHRTDSYIGVMIDDLVTKG
HCCEEEEEEEECCCCCCHHHCCCCEEEECHHHHCCCCCEEEEEECCCCHHHHHHHHHHHC
ITEPYRLFTSRAEYRLAIRSDNADRRLTQKGYDISLVSHERYSVLQGKLKSIKQLEEKLE
CCHHHHHHHCCCEEEEEEECCCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
SLTITPEQLRSYGIKISYDGIRKTALDLLGYPNIDWNKLREIWPELNMGSSVSYLHNAKA
HCCCCHHHHHHCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHCCCC
PLPVIQVADTGIQYLNDDGMDAVDTEKNVDSSVMCWNNTITDSIAKNEIYEAVAIEAKYK
CCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEECCC
PYLVRQEADMKFLQEEVNTQIPTNFNYSQIKGLSTEVIEKLQSIKPATIGIAKQIQGITP
CEEEECHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCH
AAIVSILVYLRNKKTKIAANSA
HHHHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure
MHKYDVVVVGGGHAGCEAATAAARLGASTLLITHKISTIGEMSCNPAIGGVAKGVVVREV
CCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEHHHHHCCCCCCCCCHHHCCHHEEHH
DALDGIMGRAIDRASIHSVVLNGSRGAAVWGPRAQADRKLYKKAIQEIILNYNNLTVKEE
HHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
SVDDFLIESDSNGESYIKAVITDSGERILTSRVVLTTGTFLRGVIHIGEQTTPSGRIGDK
CCCCEEEECCCCCHHEEEEEEECCCCEEEHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
PAIELANTLKKYDFKLGRLRTGTPPRLDRGTINWSVLQEQVGDNPPTPFSYLTEKINQPQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCC
VSCFITHTNEHTHRVIRENLHRSASSYLDNVIAPRYCPSIETKVKKFAEKNNHQIFLEPE
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECC
GIDDNTIYPNGISNSLPIEVQYEMIKSIKGLENAEILRPGYAVEYDYIDPRELFHTLETK
CCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCEECCCCEEEECCCCHHHHHHHHHHH
KVKGLYFAGQINGTTGYEEAAGQGIIAGINAALSLSQKSFVLHRTDSYIGVMIDDLVTKG
HCCEEEEEEEECCCCCCHHHCCCCEEEECHHHHCCCCCEEEEEECCCCHHHHHHHHHHHC
ITEPYRLFTSRAEYRLAIRSDNADRRLTQKGYDISLVSHERYSVLQGKLKSIKQLEEKLE
CCHHHHHHHCCCEEEEEEECCCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
SLTITPEQLRSYGIKISYDGIRKTALDLLGYPNIDWNKLREIWPELNMGSSVSYLHNAKA
HCCCCHHHHHHCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHCCCC
PLPVIQVADTGIQYLNDDGMDAVDTEKNVDSSVMCWNNTITDSIAKNEIYEAVAIEAKYK
CCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEECCC
PYLVRQEADMKFLQEEVNTQIPTNFNYSQIKGLSTEVIEKLQSIKPATIGIAKQIQGITP
CEEEECHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCH
AAIVSILVYLRNKKTKIAANSA
HHHHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA