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The map label for this gene is miaB

Identifier: 190571036

GI number: 190571036

Start: 685368

End: 686690

Strand: Reverse

Name: miaB

Synonym: WPa_0629

Alternate gene names: 190571036

Gene position: 686690-685368 (Counterclockwise)

Preceding gene: 190571038

Following gene: 190571034

Centisome position: 46.32

GC content: 37.94

Gene sequence:

>1323_bases
ATGAAAGGCCTATATATTAAAACTTACGGCTGTCAGATGAACGTTTATGACTCCATTTTAATGGAGAATATAGTTAAGCC
TTTAGGGTTCAACGTTGTTAGTGATGCAGAACAAGCTGATTTGGTGATATTAAATACTTGTCATATTAGAGAAAAGGCAG
CAGAAAAGCTTTACTCAGAGCTTGGAAGAATCCATTCATCACAAAAAAATAAGGAAATGACCATAGTGGTGGCTGGATGT
GTAGCGCAAGCAGAGGGAGAAGAAGTGTTCAGAAGGGCTCCTTTTGTTGATATTGTTGTTGGTCCGCAGAGTATTCCTGC
TTTACCGGAGTTGATAGTCAAAGCAAGCAGAAGTAAAGGTCATGTAATAAATACTGATTTTCCAGAAGTTGCAAAGTTTG
ATAAATTGCCGGATGAATGCTATGGCAATAGCCAGGGGTCTTCTGCGTTTCTTGCCATACAAGAAGGTTGTGATAAATTT
TGTACGTTTTGTGTGGTACCATATACACGCGGGGCTGAGTATTCACGACCAGTAAATGAAATATTTCGTGAAGCATTGAA
TTTGGTTAAAAACGGAGCAAAGGAAATTACTTTACTTGGTCAGAACGTCAATGCTTATCATGGAGAGTGCGAAGGAGAGG
TGTGGGATTTAGGAAAATTAATTAGTCACATTGCTAAAATTGAAAAGCTAGAAAGAATCCGCTACACAACTTCTCATCCA
AGAGATATGCACGAATCTCTCTACTTGGTACATGCGGAGGAATCAAAACTCATGCCGTTTGTTCACCTACCTGTGCAGTC
AGGTTCGAATAAAATATTGCATGCAATGAACAGGAAACACACTGCAGAGGAGTATTTGGGAATAATAGACAGATTGCGCA
AATTGAAACCTGAAATCGAATTTTCTTCTGATTTTATTGTTGGTTTTCCTGGAGAAACTGAAAAAGATTTTGAGGAAACT
ATGAAGTTAGTAGAAAAAGTAAAGTACGCTCAGGCTTATAGTTTTAAATATAGCCCAAGGCCAGGAACGCCAGGGGCGGA
AAGAAAAGATCAAGTACCAGAAGAGGTTAAAACAGAGCGTCTTCTTCGTTTACAGGAATTAATTAGCAAGCAACAACTTG
AATTTAATCAGAGTATGATAGGAAAAACTATTCCTGTTCTATTCAGTGATAAAAAAGGTAAACACCAAAATCAAATTATT
GGTAAAAGCCCATACATGCAATCAGTATGCGTTGATGATCCTGACGATAAATACAAAGATAAAATAGTAAATGTAAGGAT
ATTGGAAGCTCGGCAAAATAGTTTGTTGGGATGCGCTGCCTAA

Upstream 100 bases:

>100_bases
AAGGAAAGCGAGAAGGTTTGTCAAGCAATCTTTATTGGCTGTTGTACTAGAATTCAGTTTTTCGTATAATTTGCTAGAAT
TTGTGTGAAGAATTTTAAAT

Downstream 100 bases:

>100_bases
TTAGAGAAAAGTGCTCCCATGGTCTCATGTAGAAGCACTTTGTGTGTTTTTATAAGTTTATAAGCTATCTAGATTTAATC
CTTAAGGGACTACAACTCTC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKGLYIKTYGCQMNVYDSILMENIVKPLGFNVVSDAEQADLVILNTCHIREKAAEKLYSELGRIHSSQKNKEMTIVVAGC
VAQAEGEEVFRRAPFVDIVVGPQSIPALPELIVKASRSKGHVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKF
CTFCVVPYTRGAEYSRPVNEIFREALNLVKNGAKEITLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHP
RDMHESLYLVHAEESKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLGIIDRLRKLKPEIEFSSDFIVGFPGETEKDFEET
MKLVEKVKYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQELISKQQLEFNQSMIGKTIPVLFSDKKGKHQNQII
GKSPYMQSVCVDDPDDKYKDKIVNVRILEARQNSLLGCAA

Sequences:

>Translated_440_residues
MKGLYIKTYGCQMNVYDSILMENIVKPLGFNVVSDAEQADLVILNTCHIREKAAEKLYSELGRIHSSQKNKEMTIVVAGC
VAQAEGEEVFRRAPFVDIVVGPQSIPALPELIVKASRSKGHVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKF
CTFCVVPYTRGAEYSRPVNEIFREALNLVKNGAKEITLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHP
RDMHESLYLVHAEESKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLGIIDRLRKLKPEIEFSSDFIVGFPGETEKDFEET
MKLVEKVKYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQELISKQQLEFNQSMIGKTIPVLFSDKKGKHQNQII
GKSPYMQSVCVDDPDDKYKDKIVNVRILEARQNSLLGCAA
>Mature_440_residues
MKGLYIKTYGCQMNVYDSILMENIVKPLGFNVVSDAEQADLVILNTCHIREKAAEKLYSELGRIHSSQKNKEMTIVVAGC
VAQAEGEEVFRRAPFVDIVVGPQSIPALPELIVKASRSKGHVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKF
CTFCVVPYTRGAEYSRPVNEIFREALNLVKNGAKEITLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHP
RDMHESLYLVHAEESKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLGIIDRLRKLKPEIEFSSDFIVGFPGETEKDFEET
MKLVEKVKYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQELISKQQLEFNQSMIGKTIPVLFSDKKGKHQNQII
GKSPYMQSVCVDDPDDKYKDKIVNVRILEARQNSLLGCAA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=438, Percent_Identity=33.1050228310502, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI28872782, Length=438, Percent_Identity=33.1050228310502, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI93277076, Length=458, Percent_Identity=26.2008733624454, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1786882, Length=439, Percent_Identity=45.3302961275626, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787057, Length=393, Percent_Identity=30.7888040712468, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=414, Percent_Identity=35.024154589372, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=283, Percent_Identity=27.9151943462898, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356207, Length=432, Percent_Identity=32.1759259259259, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19922432, Length=471, Percent_Identity=26.963906581741, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_WOLPP (B3CLG9)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975394.1
- EnsemblBacteria:   EBWOLT00000000412
- GeneID:   6385595
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0629
- GeneTree:   EBGT00050000030823
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49678; Mature: 49678

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLYIKTYGCQMNVYDSILMENIVKPLGFNVVSDAEQADLVILNTCHIREKAAEKLYSE
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHH
LGRIHSSQKNKEMTIVVAGCVAQAEGEEVFRRAPFVDIVVGPQSIPALPELIVKASRSKG
HHHHHCCCCCCEEEEEEEECHHHCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCC
HVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCTFCVVPYTRGAEYSRPVNE
CEECCCCHHHHHHHCCCHHHCCCCCCCCEEEEHHHHHHHHHHEEECCCCCCCCCCCCHHH
IFREALNLVKNGAKEITLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHP
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
RDMHESLYLVHAEESKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLGIIDRLRKLKPEIE
HHHHHCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
FSSDFIVGFPGETEKDFEETMKLVEKVKYAQAYSFKYSPRPGTPGAERKDQVPEEVKTER
ECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHCCHHHHHHHH
LLRLQELISKQQLEFNQSMIGKTIPVLFSDKKGKHQNQIIGKSPYMQSVCVDDPDDKYKD
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
KIVNVRILEARQNSLLGCAA
HEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKGLYIKTYGCQMNVYDSILMENIVKPLGFNVVSDAEQADLVILNTCHIREKAAEKLYSE
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHH
LGRIHSSQKNKEMTIVVAGCVAQAEGEEVFRRAPFVDIVVGPQSIPALPELIVKASRSKG
HHHHHCCCCCCEEEEEEEECHHHCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCC
HVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCTFCVVPYTRGAEYSRPVNE
CEECCCCHHHHHHHCCCHHHCCCCCCCCEEEEHHHHHHHHHHEEECCCCCCCCCCCCHHH
IFREALNLVKNGAKEITLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHP
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
RDMHESLYLVHAEESKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLGIIDRLRKLKPEIE
HHHHHCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
FSSDFIVGFPGETEKDFEETMKLVEKVKYAQAYSFKYSPRPGTPGAERKDQVPEEVKTER
ECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHCCHHHHHHHH
LLRLQELISKQQLEFNQSMIGKTIPVLFSDKKGKHQNQIIGKSPYMQSVCVDDPDDKYKD
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
KIVNVRILEARQNSLLGCAA
HEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA