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The map label for this gene is gpsA

Identifier: 190570996

GI number: 190570996

Start: 629166

End: 630143

Strand: Reverse

Name: gpsA

Synonym: WPa_0584

Alternate gene names: 190570996

Gene position: 630143-629166 (Counterclockwise)

Preceding gene: 190571000

Following gene: 190570995

Centisome position: 42.51

GC content: 33.84

Gene sequence:

>978_bases
GTGGTAATATCAGTTTTAGGTGCTGGCGCATGGGGTACAGCAATTGCGATTTCATTAAGTGGTAAGAAAAATGTAATTTT
GTGGACTCGCAATAAGGCCATATTTGAATCAATTAAAGAGAAAAGAGAAAGTGACAAGCTACCTGATTGTCCAATTTCTG
ATAATGTATCAGTAAAATTAGCTATTGAAGATGCAGTGAATGCTTCAGTAATAATTCTAGCTGTTCCCACTCAGTCTCTA
AGGGAGATATGTCATCAATTGAATGATTGTAACCTAAAAAAAAATGTAGCAATAATTTTGGCTTGTAAGGGAATAGAAAA
ATCTACATTAAAATTACCTAGTGAAGTAGTCAATGAAGTTTTACCTAATAATCCTCTTGCTGTTTTTTCTGGTCCTAGCT
TTGCTATAGAAGTTGCAAGAAAATTACCATATTCGATGGTTCTTGCATGTCAAAATGAAGTATTAGGTTTAAAATTAGTA
TCAGAGCTACAGCAAGAAAATATTAAATTGTACCTTAGTAGCGATGTTATAGGAATACAGGTTTGTGCAGCGTTAAAGAA
TGTTTTTGCTATAGCATGTGGGGTTGTTCTAGGTAGGTTTGGGTTTAATGCTCATGCAGCATTAATTACGAAAAGTATGA
GTGAAATTAAGGCTTTATACTCAGCAAAAGTTGGTGACGGCAATGTAGATATAAATACACTACTTGGACCAGCATGCTTA
GGCGATTTGGTTGTGACATGCACATCATTAAATTCAAGAAATCTATCTTTTGGATCTAAAGTAGCTAATAGTAATGGTTC
TAGTGCTCAGCAGATTTTATCAGAAGGTAAGTCGGTAATTGAAGGTTTTAACACTGCTAAGTCTGCATTTGATTTAGCAG
AAAAGCTAAAGATAAAAATGCCCATATGTGAAGCGATCTATAGATTGCTATATGAAAATACTTCTATAGAAGATACTATT
TCTGTTCTTGTAAATTAG

Upstream 100 bases:

>100_bases
AGGTTAATAGATATTTAAACTATTTTATACTTTTATGTTATATTTTTAATTTACTTATTCTACAGTTTTAAATTATAAGT
AATACTTTCGGGAATATTGA

Downstream 100 bases:

>100_bases
TTTTATATTTCGGTATATTGTGAAATTTTTATATAAATTAATATCAACATGGTGGCTATCTGGCACGGTAAAACACATGC
CAGGTACTATAGGCAGCTTG

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MVISVLGAGAWGTAIAISLSGKKNVILWTRNKAIFESIKEKRESDKLPDCPISDNVSVKLAIEDAVNASVIILAVPTQSL
REICHQLNDCNLKKNVAIILACKGIEKSTLKLPSEVVNEVLPNNPLAVFSGPSFAIEVARKLPYSMVLACQNEVLGLKLV
SELQQENIKLYLSSDVIGIQVCAALKNVFAIACGVVLGRFGFNAHAALITKSMSEIKALYSAKVGDGNVDINTLLGPACL
GDLVVTCTSLNSRNLSFGSKVANSNGSSAQQILSEGKSVIEGFNTAKSAFDLAEKLKIKMPICEAIYRLLYENTSIEDTI
SVLVN

Sequences:

>Translated_325_residues
MVISVLGAGAWGTAIAISLSGKKNVILWTRNKAIFESIKEKRESDKLPDCPISDNVSVKLAIEDAVNASVIILAVPTQSL
REICHQLNDCNLKKNVAIILACKGIEKSTLKLPSEVVNEVLPNNPLAVFSGPSFAIEVARKLPYSMVLACQNEVLGLKLV
SELQQENIKLYLSSDVIGIQVCAALKNVFAIACGVVLGRFGFNAHAALITKSMSEIKALYSAKVGDGNVDINTLLGPACL
GDLVVTCTSLNSRNLSFGSKVANSNGSSAQQILSEGKSVIEGFNTAKSAFDLAEKLKIKMPICEAIYRLLYENTSIEDTI
SVLVN
>Mature_325_residues
MVISVLGAGAWGTAIAISLSGKKNVILWTRNKAIFESIKEKRESDKLPDCPISDNVSVKLAIEDAVNASVIILAVPTQSL
REICHQLNDCNLKKNVAIILACKGIEKSTLKLPSEVVNEVLPNNPLAVFSGPSFAIEVARKLPYSMVLACQNEVLGLKLV
SELQQENIKLYLSSDVIGIQVCAALKNVFAIACGVVLGRFGFNAHAALITKSMSEIKALYSAKVGDGNVDINTLLGPACL
GDLVVTCTSLNSRNLSFGSKVANSNGSSAQQILSEGKSVIEGFNTAKSAFDLAEKLKIKMPICEAIYRLLYENTSIEDTI
SVLVN

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=345, Percent_Identity=29.5652173913043, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI24307999, Length=343, Percent_Identity=25.9475218658892, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790037, Length=320, Percent_Identity=35, Blast_Score=178, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17507425, Length=355, Percent_Identity=25.3521126760563, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32564399, Length=330, Percent_Identity=25.4545454545455, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI193210136, Length=338, Percent_Identity=24.8520710059172, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32564403, Length=338, Percent_Identity=24.8520710059172, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324513, Length=351, Percent_Identity=26.2108262108262, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362270, Length=297, Percent_Identity=24.9158249158249, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45551945, Length=297, Percent_Identity=24.9158249158249, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136200, Length=348, Percent_Identity=25.5747126436782, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136204, Length=348, Percent_Identity=25.5747126436782, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17136202, Length=348, Percent_Identity=25.5747126436782, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648969, Length=258, Percent_Identity=24.4186046511628, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_WOLPP (B3CLC5)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975354.1
- ProteinModelPortal:   B3CLC5
- EnsemblBacteria:   EBWOLT00000001147
- GeneID:   6385651
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0584
- GeneTree:   EBGT00050000030863
- HOGENOM:   HBG586392
- OMA:   NVAKGIE
- ProtClustDB:   PRK14620
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 34741; Mature: 34741

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 186-186 BINDING 103-103 BINDING 103-103 BINDING 135-135 BINDING 253-253 BINDING 281-281

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISVLGAGAWGTAIAISLSGKKNVILWTRNKAIFESIKEKRESDKLPDCPISDNVSVKL
CEEEEEECCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
AIEDAVNASVIILAVPTQSLREICHQLNDCNLKKNVAIILACKGIEKSTLKLPSEVVNEV
EEECCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCHHHHHHH
LPNNPLAVFSGPSFAIEVARKLPYSMVLACQNEVLGLKLVSELQQENIKLYLSSDVIGIQ
CCCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCHHHH
VCAALKNVFAIACGVVLGRFGFNAHAALITKSMSEIKALYSAKVGDGNVDINTLLGPACL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCHHHH
GDLVVTCTSLNSRNLSFGSKVANSNGSSAQQILSEGKSVIEGFNTAKSAFDLAEKLKIKM
HHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
PICEAIYRLLYENTSIEDTISVLVN
HHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MVISVLGAGAWGTAIAISLSGKKNVILWTRNKAIFESIKEKRESDKLPDCPISDNVSVKL
CEEEEEECCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
AIEDAVNASVIILAVPTQSLREICHQLNDCNLKKNVAIILACKGIEKSTLKLPSEVVNEV
EEECCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCHHHHHHH
LPNNPLAVFSGPSFAIEVARKLPYSMVLACQNEVLGLKLVSELQQENIKLYLSSDVIGIQ
CCCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEEECCCHHHH
VCAALKNVFAIACGVVLGRFGFNAHAALITKSMSEIKALYSAKVGDGNVDINTLLGPACL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCHHHH
GDLVVTCTSLNSRNLSFGSKVANSNGSSAQQILSEGKSVIEGFNTAKSAFDLAEKLKIKM
HHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
PICEAIYRLLYENTSIEDTISVLVN
HHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA