Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 190570964

GI number: 190570964

Start: 589072

End: 590343

Strand: Reverse

Name: purD [H]

Synonym: WPa_0550

Alternate gene names: 190570964

Gene position: 590343-589072 (Counterclockwise)

Preceding gene: 190570965

Following gene: 190570963

Centisome position: 39.82

GC content: 36.4

Gene sequence:

>1272_bases
ATGAAGGTTCTGGTTATAGGTTCTGGTGGGCGTGAGCATGCTTTACTTTGGGCTTTAAAGAAGTCTCCTACCTTGACTAA
GTTATATGTTACTCCTGGTCGCCCGGCCATGGAAAATTTTGGAGTTCTTGTGAATATAAATATTCAAGATTCAGTGGATG
TTACACAATTTTGCAAGAAAGAAAATATAGAGCTAGTGATTATTGGTCCAGAGCAGCCGATAATTAATGGGCTTGCTGAT
GATTTAACTGCGGAGGGGATAAACGTTTTTGCTCCAAGTCAAGCAGCTGCAAAACTTGAGGCATCAAAGTCTTTTACGAA
GGAACTATGTAAACAATATGGTATACCGACCGCCAAGTATGAACGTTTTATTGATGAAAGGTTAGCTAAAAATTTTGTCC
GTAGTAATAAAATAAAATTGCCGCTTGTAATTAAAGCAAATGGAATTGCAGCAGGGAAAGGTGTCATAATATGCCACACA
GAAAATGAGGCTTTTTCAGCAATAGATTCAATGCTAGTGGAGAAAAATCTTGGTGAGTCAGGTGAAGAAATAATCATAGA
AGAGTTTTTAATTGGAGAGGAAGTAAGTTTTTTTGCTCTTATTGATGGGTTGAAAGTAGTAACCTTTGGTTGTGCAAGAG
ATTATAAGAAAGTTGATGAAAATAACGAGGGCCAAAATACTGGAGGCATGGGGTCATATTCATCACCTTCGATTATAAGT
AAGGACATGGAACAAAAAATTATACAAAAAATAATATATCCTACAATTCAAGCACTGACTAACATGGGAACATCCTATAA
AGGAGTACTCTTTGCTGGTTTAATGATTTGCAAAGATAGTCCAAAGCTTCTTGAGTATAATGTTAGGTTTGGGGATCCGG
AAATACAATCTATTTTACCTAGACTTGATCCAAATTGTGATTTGTTAAAGTTGATGTTGTCAGTTGCAGAAGGCAAGTTA
AATACAAAAATGGTGGAGTTTAATCACAAAACTACAGTTTGTGTAGTTGTTGCAAGTAAAGGCTATCCTGGTGATTATCA
AAAAGGAGAGGTAATTAAAGGATTGGATAAAATTGAAAACATTCCTGGTGTGCTAGTTTTTCATGCTGGTACTAAGTTGG
ATGAAAGTGGTAATTGGATTTCCGATGGCGGAAGAGTGCTAAATATAGTAGGAGAAGGAAACACTGTGGAAGAAGCTAAA
AGTAAAGTGTACTCAGCGTTAAATTTTCTAGAATGGTCAGGTGGGTTCTTCAGGTATGATATTGGTAGTTAA

Upstream 100 bases:

>100_bases
GGGTCAGTTACAATACCAAACGTGTTGCAAAGATACATGAGTAATGATACTGTTATAAGCAAACAATAATTTTGACATAT
AGAAAAGCGAGGAAATAAAG

Downstream 100 bases:

>100_bases
TTTAGCTCATGCAGAAACAAAAAAAGTCACTTTGTAAGCAGTAAACTAAAGAAAAACATGATATCTGTTCAGATTCATGT
GAAACCAGTGCTTCTTTTCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVLVIGSGGREHALLWALKKSPTLTKLYVTPGRPAMENFGVLVNINIQDSVDVTQFCKKENIELVIIGPEQPIINGLAD
DLTAEGINVFAPSQAAAKLEASKSFTKELCKQYGIPTAKYERFIDERLAKNFVRSNKIKLPLVIKANGIAAGKGVIICHT
ENEAFSAIDSMLVEKNLGESGEEIIIEEFLIGEEVSFFALIDGLKVVTFGCARDYKKVDENNEGQNTGGMGSYSSPSIIS
KDMEQKIIQKIIYPTIQALTNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPEIQSILPRLDPNCDLLKLMLSVAEGKL
NTKMVEFNHKTTVCVVVASKGYPGDYQKGEVIKGLDKIENIPGVLVFHAGTKLDESGNWISDGGRVLNIVGEGNTVEEAK
SKVYSALNFLEWSGGFFRYDIGS

Sequences:

>Translated_423_residues
MKVLVIGSGGREHALLWALKKSPTLTKLYVTPGRPAMENFGVLVNINIQDSVDVTQFCKKENIELVIIGPEQPIINGLAD
DLTAEGINVFAPSQAAAKLEASKSFTKELCKQYGIPTAKYERFIDERLAKNFVRSNKIKLPLVIKANGIAAGKGVIICHT
ENEAFSAIDSMLVEKNLGESGEEIIIEEFLIGEEVSFFALIDGLKVVTFGCARDYKKVDENNEGQNTGGMGSYSSPSIIS
KDMEQKIIQKIIYPTIQALTNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPEIQSILPRLDPNCDLLKLMLSVAEGKL
NTKMVEFNHKTTVCVVVASKGYPGDYQKGEVIKGLDKIENIPGVLVFHAGTKLDESGNWISDGGRVLNIVGEGNTVEEAK
SKVYSALNFLEWSGGFFRYDIGS
>Mature_423_residues
MKVLVIGSGGREHALLWALKKSPTLTKLYVTPGRPAMENFGVLVNINIQDSVDVTQFCKKENIELVIIGPEQPIINGLAD
DLTAEGINVFAPSQAAAKLEASKSFTKELCKQYGIPTAKYERFIDERLAKNFVRSNKIKLPLVIKANGIAAGKGVIICHT
ENEAFSAIDSMLVEKNLGESGEEIIIEEFLIGEEVSFFALIDGLKVVTFGCARDYKKVDENNEGQNTGGMGSYSSPSIIS
KDMEQKIIQKIIYPTIQALTNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPEIQSILPRLDPNCDLLKLMLSVAEGKL
NTKMVEFNHKTTVCVVVASKGYPGDYQKGEVIKGLDKIENIPGVLVFHAGTKLDESGNWISDGGRVLNIVGEGNTVEEAK
SKVYSALNFLEWSGGFFRYDIGS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=40.8018867924528, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=40.8018867924528, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=40.8018867924528, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=40.8018867924528, Blast_Score=318, Evalue=5e-87,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=42.8904428904429, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=38.1733021077283, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321203, Length=436, Percent_Identity=38.0733944954128, Blast_Score=302, Evalue=6e-83,
Organism=Drosophila melanogaster, GI62471619, Length=428, Percent_Identity=38.3177570093458, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=38.2284382284382, Blast_Score=277, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46292; Mature: 46292

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGSGGREHALLWALKKSPTLTKLYVTPGRPAMENFGVLVNINIQDSVDVTQFCKK
CEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCHHHCCEEEEEEECCCCCHHHHHHH
ENIELVIIGPEQPIINGLADDLTAEGINVFAPSQAAAKLEASKSFTKELCKQYGIPTAKY
CCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
ERFIDERLAKNFVRSNKIKLPLVIKANGIAAGKGVIICHTENEAFSAIDSMLVEKNLGES
HHHHHHHHHHHHHHCCCEEEEEEEEECCEECCCCEEEEECCCHHHHHHHHHHHHHCCCCC
GEEIIIEEFLIGEEVSFFALIDGLKVVTFGCARDYKKVDENNEGQNTGGMGSYSSPSIIS
CCCEEHHHHHCCCCCHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KDMEQKIIQKIIYPTIQALTNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPEIQSILP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCEEEEEEECCCHHHHHHHH
RLDPNCDLLKLMLSVAEGKLNTKMVEFNHKTTVCVVVASKGYPGDYQKGEVIKGLDKIEN
CCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHC
IPGVLVFHAGTKLDESGNWISDGGRVLNIVGEGNTVEEAKSKVYSALNFLEWSGGFFRYD
CCCEEEEECCCCCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEE
IGS
CCC
>Mature Secondary Structure
MKVLVIGSGGREHALLWALKKSPTLTKLYVTPGRPAMENFGVLVNINIQDSVDVTQFCKK
CEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCHHHCCEEEEEEECCCCCHHHHHHH
ENIELVIIGPEQPIINGLADDLTAEGINVFAPSQAAAKLEASKSFTKELCKQYGIPTAKY
CCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
ERFIDERLAKNFVRSNKIKLPLVIKANGIAAGKGVIICHTENEAFSAIDSMLVEKNLGES
HHHHHHHHHHHHHHCCCEEEEEEEEECCEECCCCEEEEECCCHHHHHHHHHHHHHCCCCC
GEEIIIEEFLIGEEVSFFALIDGLKVVTFGCARDYKKVDENNEGQNTGGMGSYSSPSIIS
CCCEEHHHHHCCCCCHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KDMEQKIIQKIIYPTIQALTNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPEIQSILP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCEEEEEEECCCHHHHHHHH
RLDPNCDLLKLMLSVAEGKLNTKMVEFNHKTTVCVVVASKGYPGDYQKGEVIKGLDKIEN
CCCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHC
IPGVLVFHAGTKLDESGNWISDGGRVLNIVGEGNTVEEAKSKVYSALNFLEWSGGFFRYD
CCCEEEEECCCCCCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEE
IGS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]