Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 190570889

GI number: 190570889

Start: 502720

End: 504675

Strand: Reverse

Name: ligA

Synonym: WPa_0466

Alternate gene names: 190570889

Gene position: 504675-502720 (Counterclockwise)

Preceding gene: 190570891

Following gene: 190570886

Centisome position: 34.04

GC content: 34.46

Gene sequence:

>1956_bases
ATGACTGACTTGGAAAAGATGAGAGAAAAACTGCAAAATCAAATTAATTATCATAATATCTTATATTATCAAAAAAGTAA
GCCAGAGATAAGTGATGCTGAATATGATGAACTGAAAAAAAAGTTAGCTGCAATAGAGCCAGAAGCTTATGCAACACAAG
ATAGTGTTGGTGCTCCGCCTGATGAGAGATTTTCTAAGGTGGAACATCAAGAACCTATGCTTTCTCTTGAGAATGCTTAT
GATGAACAAGGTGTAGAAAAATTTTTGTCTAAAATAAAGAGATTTTTAATTGCAGATGAAATAGAGATATTATGTGAGCC
AAAAATTGATGGATTGTCATTTTCTGCAATTTATGAAGATGGGAAATTTGTTAAAGCTGCAACTCGAGGTGACGGTTTTG
TAGGAGAGGATGTAACTCACAATGTTGCAACAATAAAAGGCTTTCCTAAGTTTTTACAAGATGTGCAAGGAAGACTAGAA
GTAAGGGGTGAAATATATATCAGTAACAGCGACTTTTTAAAGTTAAATGAAAATAATGAATTCGCTAATCCTCGAAATGC
AGCTGCTGGTTCTTTAAAGCAATTGAATGCGAACATTACAGCAAAAAGGCCTCTTAGATATTTTGCTTATTCTTTAATTG
GTGGAATAGAGAAAAGTCAAAGTGAAGTACTAGAAAAACTTGAGAAACTTGGTTTTTGCGTAAATGAGCATCGTTCCTTA
ACAAGTAGTTTGAATGGAATGCTAAAGTTCTATAACGAGATCTATGATTGTCGTTATAACTTGGATTATGATATCGATGG
GATAGTCTATAAAGTGAATGATTTGGTGCTACAAAGTCGTCTGGGAAGTACACACAAAGCGCCACGCTCAGCGCTTGCAT
ATAAGTTTTCTGCGATTTATGCGAAGACAAAGTTAAATAAAATATTTATACAGGTGGGTAGAACGGGGGTTTTAACTCCA
GTTGCAGATTTGGTACCAGTTAATATTGGCGGAGTGCTAGTTAGCAGAGCAAGTCTACATAACCAAGATGAGATAAAACG
TAAAGATATAAGAGAAGGAGATGTTGTAACAATTAAAAGAGCTGGAGATGTAATTCCTCAAATTGTCAAAGTAGATGAAG
GTTCTCGTCATACAAATATGCCTGAATTTGTTTTTCCTGACATATGTCCTGAATGTGGTAGTAAAGTGCAGATTGAAGGA
GTGGCAGTAAGATGTCCTGAAGAATTTAATTGCAAGGCTCAAATAGTAGAAAAACTAAAACATTTTGTGTCAAAAGATGC
ATTTGACATTGTTGGCCTTGGTGAAAAACAAATAAAGTTTTTTTATGATCTGGAACTAATAAGACAAATTCCAGACATTT
TTATTTTAGAGGAAAGATTAAAAGAGTTTTCCTTAAAAGAGCATCATGGTTGGGGCGAGAAGTCAATAGCTAATCTATTA
AGTGCTATACAAAGCAGAAGAGTAATCTCTCTAGATAGGTTCATATTTTCTTTGGGTATCAGATTTATCGGCCAAGTTGT
AGCAGAATTGCTCGCAAATTATTATGTATCTTATGATAACTGGTATAATTCGATGTCATCAAATGATGTTGAGTTGGTAG
GTATAGATGGTATAGGAGAAAAAGTAGCTGAATCTTTAAAATCGTTTTTTTCTCAGGAACGTAACATTAAAATGTTAAAT
GATCTTACTGCATATCTGCAAATTCTTCCCGTGAGCTCCAACTCTAGCGATTCTTTTTTGAATAACAAAATTATAGTGTT
TACTGGTAAGCTACGTGCTATGAGTAGAGGGGAGGCAAAAGTAAGAGCTAAAGTTTTAGGGGCAAAGATCAGTTCAAGCC
TTTCTACTAAAACTGACTATTTAATTGCAGGAGAAGATCCAGGTTCAAAATATAAAAAAGCAATGGAATTGGGTGTGGAA
ATTTTAGATGAAGAACAGTGGAACAGATTGTGCTAA

Upstream 100 bases:

>100_bases
TATATTAATATTAGTATTGCTTTTCAGGGCAGTAGTATAAATTTATAGTACCTTTTGAAAAAATTCATGACTTGACGGCA
AAGAGCATTATATTGGTATC

Downstream 100 bases:

>100_bases
ACACAACTTAATCTGACGGGAATGAATTAACTGATAGAAGAATTGAAAACTAATATCAGACTCTTATTTAATACAATTTA
TAGATGCGACAGAACCCTAA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MTDLEKMREKLQNQINYHNILYYQKSKPEISDAEYDELKKKLAAIEPEAYATQDSVGAPPDERFSKVEHQEPMLSLENAY
DEQGVEKFLSKIKRFLIADEIEILCEPKIDGLSFSAIYEDGKFVKAATRGDGFVGEDVTHNVATIKGFPKFLQDVQGRLE
VRGEIYISNSDFLKLNENNEFANPRNAAAGSLKQLNANITAKRPLRYFAYSLIGGIEKSQSEVLEKLEKLGFCVNEHRSL
TSSLNGMLKFYNEIYDCRYNLDYDIDGIVYKVNDLVLQSRLGSTHKAPRSALAYKFSAIYAKTKLNKIFIQVGRTGVLTP
VADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDVVTIKRAGDVIPQIVKVDEGSRHTNMPEFVFPDICPECGSKVQIEG
VAVRCPEEFNCKAQIVEKLKHFVSKDAFDIVGLGEKQIKFFYDLELIRQIPDIFILEERLKEFSLKEHHGWGEKSIANLL
SAIQSRRVISLDRFIFSLGIRFIGQVVAELLANYYVSYDNWYNSMSSNDVELVGIDGIGEKVAESLKSFFSQERNIKMLN
DLTAYLQILPVSSNSSDSFLNNKIIVFTGKLRAMSRGEAKVRAKVLGAKISSSLSTKTDYLIAGEDPGSKYKKAMELGVE
ILDEEQWNRLC

Sequences:

>Translated_651_residues
MTDLEKMREKLQNQINYHNILYYQKSKPEISDAEYDELKKKLAAIEPEAYATQDSVGAPPDERFSKVEHQEPMLSLENAY
DEQGVEKFLSKIKRFLIADEIEILCEPKIDGLSFSAIYEDGKFVKAATRGDGFVGEDVTHNVATIKGFPKFLQDVQGRLE
VRGEIYISNSDFLKLNENNEFANPRNAAAGSLKQLNANITAKRPLRYFAYSLIGGIEKSQSEVLEKLEKLGFCVNEHRSL
TSSLNGMLKFYNEIYDCRYNLDYDIDGIVYKVNDLVLQSRLGSTHKAPRSALAYKFSAIYAKTKLNKIFIQVGRTGVLTP
VADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDVVTIKRAGDVIPQIVKVDEGSRHTNMPEFVFPDICPECGSKVQIEG
VAVRCPEEFNCKAQIVEKLKHFVSKDAFDIVGLGEKQIKFFYDLELIRQIPDIFILEERLKEFSLKEHHGWGEKSIANLL
SAIQSRRVISLDRFIFSLGIRFIGQVVAELLANYYVSYDNWYNSMSSNDVELVGIDGIGEKVAESLKSFFSQERNIKMLN
DLTAYLQILPVSSNSSDSFLNNKIIVFTGKLRAMSRGEAKVRAKVLGAKISSSLSTKTDYLIAGEDPGSKYKKAMELGVE
ILDEEQWNRLC
>Mature_650_residues
TDLEKMREKLQNQINYHNILYYQKSKPEISDAEYDELKKKLAAIEPEAYATQDSVGAPPDERFSKVEHQEPMLSLENAYD
EQGVEKFLSKIKRFLIADEIEILCEPKIDGLSFSAIYEDGKFVKAATRGDGFVGEDVTHNVATIKGFPKFLQDVQGRLEV
RGEIYISNSDFLKLNENNEFANPRNAAAGSLKQLNANITAKRPLRYFAYSLIGGIEKSQSEVLEKLEKLGFCVNEHRSLT
SSLNGMLKFYNEIYDCRYNLDYDIDGIVYKVNDLVLQSRLGSTHKAPRSALAYKFSAIYAKTKLNKIFIQVGRTGVLTPV
ADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDVVTIKRAGDVIPQIVKVDEGSRHTNMPEFVFPDICPECGSKVQIEGV
AVRCPEEFNCKAQIVEKLKHFVSKDAFDIVGLGEKQIKFFYDLELIRQIPDIFILEERLKEFSLKEHHGWGEKSIANLLS
AIQSRRVISLDRFIFSLGIRFIGQVVAELLANYYVSYDNWYNSMSSNDVELVGIDGIGEKVAESLKSFFSQERNIKMLND
LTAYLQILPVSSNSSDSFLNNKIIVFTGKLRAMSRGEAKVRAKVLGAKISSSLSTKTDYLIAGEDPGSKYKKAMELGVEI
LDEEQWNRLC

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=676, Percent_Identity=41.7159763313609, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI87082305, Length=501, Percent_Identity=22.7544910179641, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_WOLPP (B3CPS1)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975247.1
- EnsemblBacteria:   EBWOLT00000000461
- GeneID:   6385443
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0466
- GeneTree:   EBGT00050000031316
- HOGENOM:   HBG620317
- OMA:   FHELKAL
- ProtClustDB:   CLSK739957
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73490; Mature: 73359

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 108-108 BINDING 106-106 BINDING 129-129 BINDING 164-164 BINDING 271-271 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLEKMREKLQNQINYHNILYYQKSKPEISDAEYDELKKKLAAIEPEAYATQDSVGAPP
CCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCC
DERFSKVEHQEPMLSLENAYDEQGVEKFLSKIKRFLIADEIEILCEPKIDGLSFSAIYED
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCEEEEEEECC
GKFVKAATRGDGFVGEDVTHNVATIKGFPKFLQDVQGRLEVRGEIYISNSDFLKLNENNE
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEECCCCC
FANPRNAAAGSLKQLNANITAKRPLRYFAYSLIGGIEKSQSEVLEKLEKLGFCVNEHRSL
CCCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
TSSLNGMLKFYNEIYDCRYNLDYDIDGIVYKVNDLVLQSRLGSTHKAPRSALAYKFSAIY
HHHHHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AKTKLNKIFIQVGRTGVLTPVADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDVVTIKR
HHHHHHHEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEC
AGDVIPQIVKVDEGSRHTNMPEFVFPDICPECGSKVQIEGVAVRCPEEFNCKAQIVEKLK
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEEEEEECCCCCCCHHHHHHHHH
HFVSKDAFDIVGLGEKQIKFFYDLELIRQIPDIFILEERLKEFSLKEHHGWGEKSIANLL
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHH
SAIQSRRVISLDRFIFSLGIRFIGQVVAELLANYYVSYDNWYNSMSSNDVELVGIDGIGE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCH
KVAESLKSFFSQERNIKMLNDLTAYLQILPVSSNSSDSFLNNKIIVFTGKLRAMSRGEAK
HHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCHHCCEEEEEECCHHHCCCCCHH
VRAKVLGAKISSSLSTKTDYLIAGEDPGSKYKKAMELGVEILDEEQWNRLC
HHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
TDLEKMREKLQNQINYHNILYYQKSKPEISDAEYDELKKKLAAIEPEAYATQDSVGAPP
CCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCC
DERFSKVEHQEPMLSLENAYDEQGVEKFLSKIKRFLIADEIEILCEPKIDGLSFSAIYED
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCEEEEEEECC
GKFVKAATRGDGFVGEDVTHNVATIKGFPKFLQDVQGRLEVRGEIYISNSDFLKLNENNE
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEECCCCC
FANPRNAAAGSLKQLNANITAKRPLRYFAYSLIGGIEKSQSEVLEKLEKLGFCVNEHRSL
CCCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
TSSLNGMLKFYNEIYDCRYNLDYDIDGIVYKVNDLVLQSRLGSTHKAPRSALAYKFSAIY
HHHHHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AKTKLNKIFIQVGRTGVLTPVADLVPVNIGGVLVSRASLHNQDEIKRKDIREGDVVTIKR
HHHHHHHEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEEEEC
AGDVIPQIVKVDEGSRHTNMPEFVFPDICPECGSKVQIEGVAVRCPEEFNCKAQIVEKLK
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEEEEEECCCCCCCHHHHHHHHH
HFVSKDAFDIVGLGEKQIKFFYDLELIRQIPDIFILEERLKEFSLKEHHGWGEKSIANLL
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHH
SAIQSRRVISLDRFIFSLGIRFIGQVVAELLANYYVSYDNWYNSMSSNDVELVGIDGIGE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCH
KVAESLKSFFSQERNIKMLNDLTAYLQILPVSSNSSDSFLNNKIIVFTGKLRAMSRGEAK
HHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCHHCCEEEEEECCHHHCCCCCHH
VRAKVLGAKISSSLSTKTDYLIAGEDPGSKYKKAMELGVEILDEEQWNRLC
HHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA