Click here to switch to the map view.

The map label for this gene is clpB [H]

Identifier: 190570794

GI number: 190570794

Start: 375604

End: 378165

Strand: Direct

Name: clpB [H]

Synonym: WPa_0355

Alternate gene names: 190570794

Gene position: 375604-378165 (Clockwise)

Preceding gene: 190570793

Following gene: 190570795

Centisome position: 25.34

GC content: 37.43

Gene sequence:

>2562_bases
ATGGACTTGAATAAATTTACCGAAAAAGCAAAAAGCCTAATCCAGAGAGCTCAAATGAAAGCATTGGGAGCTGGGCATCA
GATTTTTATGCCTGAACATTTACTTAAAGTAATGCTTGAAGATGAGTCAGGTTTAGTTCAGGATTTGATAGGCACCTGTG
GTGGAAATGTTCAGAATATTTCTGATGCTGTGAGTAGTACAATTAAAAAGTTTCCAGTGGTTGGAGGACCAGGTAGTGTT
GGACTTCAGCTTTCAAGAGAGGTAGCAAAAGTTTTTGATGATTCAATTGGTATTGCGAGGAAAAATAAAGACACATTTGT
TACTGTTGAGCGGTTGTTGCAGGGCCTTGCTGCACAAAAAGATGAAATTGTAGGTAAAATTTTAGCAGAAGGTGGTGTAA
CACCGCAAAAATTAAATTCAGTTATTGCAGAAATGAGAAAAGGTGGCAGTGCAGACTCTCCAAATAGCGAGGAAAAATTA
AATGCGGCAAAGAGGTATACAAAAGATATTACAGAGCTTGCTATGCAAGGTAAGCTTGATCCTGTAATTGGTCGTGATGA
GGAAATCAGAAGAACTATGCAGGTATCGTTGAGGCGAACAAAAAATAATCCAGTACTGATAGGTGAACCTGGTGTTGGAA
AAACTGCAATAGTTGAAGGTTTAGCAAACAGAATTGTTGCAAATGATGTACCACTTGGTTTGCGTGATGCAAAAGTTTTA
GCTTTGGATCTTGGTGCATTAATTGCTGGAACAAAATTTAGAGGGGAGTTTGAAGAGAGACTAAAAGCAGTGATTAATGA
GATTTTAAAAGCAGAGGGCAAAGTTATTTTGTTTATAGATGAGCTTCATACTTTAGTGGGAGCAGGAGCGACAAGTGGTG
CGATGGATGCTTCAAATTTGCTCAAGCCTGCTCTTGCGCGTGGAGAAATTCGCTGTATAGGGGCTACAACTTTGGATGAG
TATCGTCAACATATAGAGAAAGACCCTGCGCTTGCAAGGCGTTTTCAGCCTGTGTTTATTTCTCAACCGACTGAGACTGA
TACTATTTCAATACTGAGGGGTTTGAAGGAGAGGTATGAGGTGCACCATGGTATTAGAATCACAGATGGTGCAATAATCG
CTGCTGCAACGCTTTCTAATAGATATATAACAGATAGGTTTTTACCTGATAAAGCGATCGATTTAATTGATGAAGCAGCA
AGTAGGGTTAGAATTGAAATGGACAGCAAGCCTGAGGTTGTTGATGAGCTTGAAAGAAAGATTATACAGCTGAAAATTGA
GTCAGAGGCTTTGAAAAAAGAAAGTGATGAAAACTCTAAGCAACGTTTAAAAAAGATAAATGAGGAAATTGAGAGTCTAA
ACAGTAAATTTGCTGACTTAAGCAGTAAATGGCAGATGGAAAAGAATAAAATAGCTAAAATACAAGAAACTGCTGAAAAA
TTGGATAATGCGAGGAAAGAGCTAGAATTGGCTCAGCGGAATGGAAATTTAGGAAGGGCAGGGGAATTGATGTATGGTGT
GATTCCTCAACTTGAAAATGAGTTAAAAAATCAGGAAAAAGTTACTGATAGTTTCCTAAAGAAAGAAGTTACTGAGAGTG
ATATTGCAAATATTGTTTCAAAATGGACAGGCATTCCAGTTGATAACATGATGCATAGTGAAAAGGAAAAGCTCCTTAAC
ATGGAAAATGAAATAGGAAAAAGAGTAATAGGGCAGAGAGATGCAATTGAAGCGATAAGTAATGCAGTAAGGCGCTCCCG
TTCTGGTGTTCAGGATACTAATCGACCTTTTGGTTCATTTTTATTCTTAGGTCCAACCGGAGTTGGAAAAACAGAGTTAG
CAAAATCTCTTGCCGAATTTTTGTTTGATGATCAATCAGCACTTCTACGCTTTGATATGTCAGAGTATATGGAAAAACAC
TCCGTTTCAAAGTTAATTGGTGCGCCTCCAGGATATGTTGGTTATGAACAAGGTGGCAGATTAACCGAAGCAGTAAGGAG
AAGACCATATCAGGTAATTTTGTTTGATGAGATAGAAAAGGCAAATCCAGATATATTTAATATACTACTGCAGATTTTGG
ATGAAGGTAGACTAACCGATAGCCATGGCAAATTAATTGATTTTCGTAATACCATACTAATTTTAACTTCTAACCTTGGA
GCTGAGATGATGCTAAGAGGAGCTACCGAATCTGTGAAAGATGAGGTGATGAAAATAGTTAAGTCAGCGTTTCGTCCAGA
ATTTTTAAATAGACTGGATGAAATTATTATATTCCACAGCTTAACCAAAGATGACATTGATAAAATTATAGATGTTCAAT
TCTCTTATTTACAAAAGATGCTTGCTAAGCGTAAACTGAGTATTAGTTTATCGCAAGAAGCTAAAGGACTAATAGCACAA
ACTGGATATGACCCTGAATATGGAGCGCGGCCTCTGAAAAGGGTAATACAAGAATGCATTCAAAATAATCTAGCTAAGTT
AGTTTTATCTGGGGAAATAGTAGAAGGAGATGAGCTAATAGTGTATACTTCTGGTAACGAAATCTTAGTTAAAAAGATCT
AG

Upstream 100 bases:

>100_bases
TGGATTTTAATCCATAGTGGTTGAATGCTGAATGACTTTAAGTTTAAAATTGAGAAAGTAGTTGTTATATATATCAGCAA
GTATGAGTTAAATTTGTATT

Downstream 100 bases:

>100_bases
ATTCGCTGTGTATTTTTGTTTTGAAAAATGTGTATAAATCATAATGTTAATGTTGAGTCTTTATTTAAGCTAGTTGATCA
AGGGAATAAAAAAGAGAAAG

Product: ATP-dependent Clp protease, ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 853; Mature: 853

Protein sequence:

>853_residues
MDLNKFTEKAKSLIQRAQMKALGAGHQIFMPEHLLKVMLEDESGLVQDLIGTCGGNVQNISDAVSSTIKKFPVVGGPGSV
GLQLSREVAKVFDDSIGIARKNKDTFVTVERLLQGLAAQKDEIVGKILAEGGVTPQKLNSVIAEMRKGGSADSPNSEEKL
NAAKRYTKDITELAMQGKLDPVIGRDEEIRRTMQVSLRRTKNNPVLIGEPGVGKTAIVEGLANRIVANDVPLGLRDAKVL
ALDLGALIAGTKFRGEFEERLKAVINEILKAEGKVILFIDELHTLVGAGATSGAMDASNLLKPALARGEIRCIGATTLDE
YRQHIEKDPALARRFQPVFISQPTETDTISILRGLKERYEVHHGIRITDGAIIAAATLSNRYITDRFLPDKAIDLIDEAA
SRVRIEMDSKPEVVDELERKIIQLKIESEALKKESDENSKQRLKKINEEIESLNSKFADLSSKWQMEKNKIAKIQETAEK
LDNARKELELAQRNGNLGRAGELMYGVIPQLENELKNQEKVTDSFLKKEVTESDIANIVSKWTGIPVDNMMHSEKEKLLN
MENEIGKRVIGQRDAIEAISNAVRRSRSGVQDTNRPFGSFLFLGPTGVGKTELAKSLAEFLFDDQSALLRFDMSEYMEKH
SVSKLIGAPPGYVGYEQGGRLTEAVRRRPYQVILFDEIEKANPDIFNILLQILDEGRLTDSHGKLIDFRNTILILTSNLG
AEMMLRGATESVKDEVMKIVKSAFRPEFLNRLDEIIIFHSLTKDDIDKIIDVQFSYLQKMLAKRKLSISLSQEAKGLIAQ
TGYDPEYGARPLKRVIQECIQNNLAKLVLSGEIVEGDELIVYTSGNEILVKKI

Sequences:

>Translated_853_residues
MDLNKFTEKAKSLIQRAQMKALGAGHQIFMPEHLLKVMLEDESGLVQDLIGTCGGNVQNISDAVSSTIKKFPVVGGPGSV
GLQLSREVAKVFDDSIGIARKNKDTFVTVERLLQGLAAQKDEIVGKILAEGGVTPQKLNSVIAEMRKGGSADSPNSEEKL
NAAKRYTKDITELAMQGKLDPVIGRDEEIRRTMQVSLRRTKNNPVLIGEPGVGKTAIVEGLANRIVANDVPLGLRDAKVL
ALDLGALIAGTKFRGEFEERLKAVINEILKAEGKVILFIDELHTLVGAGATSGAMDASNLLKPALARGEIRCIGATTLDE
YRQHIEKDPALARRFQPVFISQPTETDTISILRGLKERYEVHHGIRITDGAIIAAATLSNRYITDRFLPDKAIDLIDEAA
SRVRIEMDSKPEVVDELERKIIQLKIESEALKKESDENSKQRLKKINEEIESLNSKFADLSSKWQMEKNKIAKIQETAEK
LDNARKELELAQRNGNLGRAGELMYGVIPQLENELKNQEKVTDSFLKKEVTESDIANIVSKWTGIPVDNMMHSEKEKLLN
MENEIGKRVIGQRDAIEAISNAVRRSRSGVQDTNRPFGSFLFLGPTGVGKTELAKSLAEFLFDDQSALLRFDMSEYMEKH
SVSKLIGAPPGYVGYEQGGRLTEAVRRRPYQVILFDEIEKANPDIFNILLQILDEGRLTDSHGKLIDFRNTILILTSNLG
AEMMLRGATESVKDEVMKIVKSAFRPEFLNRLDEIIIFHSLTKDDIDKIIDVQFSYLQKMLAKRKLSISLSQEAKGLIAQ
TGYDPEYGARPLKRVIQECIQNNLAKLVLSGEIVEGDELIVYTSGNEILVKKI
>Mature_853_residues
MDLNKFTEKAKSLIQRAQMKALGAGHQIFMPEHLLKVMLEDESGLVQDLIGTCGGNVQNISDAVSSTIKKFPVVGGPGSV
GLQLSREVAKVFDDSIGIARKNKDTFVTVERLLQGLAAQKDEIVGKILAEGGVTPQKLNSVIAEMRKGGSADSPNSEEKL
NAAKRYTKDITELAMQGKLDPVIGRDEEIRRTMQVSLRRTKNNPVLIGEPGVGKTAIVEGLANRIVANDVPLGLRDAKVL
ALDLGALIAGTKFRGEFEERLKAVINEILKAEGKVILFIDELHTLVGAGATSGAMDASNLLKPALARGEIRCIGATTLDE
YRQHIEKDPALARRFQPVFISQPTETDTISILRGLKERYEVHHGIRITDGAIIAAATLSNRYITDRFLPDKAIDLIDEAA
SRVRIEMDSKPEVVDELERKIIQLKIESEALKKESDENSKQRLKKINEEIESLNSKFADLSSKWQMEKNKIAKIQETAEK
LDNARKELELAQRNGNLGRAGELMYGVIPQLENELKNQEKVTDSFLKKEVTESDIANIVSKWTGIPVDNMMHSEKEKLLN
MENEIGKRVIGQRDAIEAISNAVRRSRSGVQDTNRPFGSFLFLGPTGVGKTELAKSLAEFLFDDQSALLRFDMSEYMEKH
SVSKLIGAPPGYVGYEQGGRLTEAVRRRPYQVILFDEIEKANPDIFNILLQILDEGRLTDSHGKLIDFRNTILILTSNLG
AEMMLRGATESVKDEVMKIVKSAFRPEFLNRLDEIIIFHSLTKDDIDKIIDVQFSYLQKMLAKRKLSISLSQEAKGLIAQ
TGYDPEYGARPLKRVIQECIQNNLAKLVLSGEIVEGDELIVYTSGNEILVKKI

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=300, Percent_Identity=36, Blast_Score=199, Evalue=8e-51,
Organism=Escherichia coli, GI1788943, Length=843, Percent_Identity=54.9228944246738, Blast_Score=936, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=319, Percent_Identity=45.4545454545455, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=52.8913963328632, Blast_Score=765, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=855, Percent_Identity=41.6374269005848, Blast_Score=637, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 94719; Mature: 94719

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNKFTEKAKSLIQRAQMKALGAGHQIFMPEHLLKVMLEDESGLVQDLIGTCGGNVQNI
CCHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCHHHHHHHHCCCCHHHH
SDAVSSTIKKFPVVGGPGSVGLQLSREVAKVFDDSIGIARKNKDTFVTVERLLQGLAAQK
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DEIVGKILAEGGVTPQKLNSVIAEMRKGGSADSPNSEEKLNAAKRYTKDITELAMQGKLD
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PVIGRDEEIRRTMQVSLRRTKNNPVLIGEPGVGKTAIVEGLANRIVANDVPLGLRDAKVL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
ALDLGALIAGTKFRGEFEERLKAVINEILKAEGKVILFIDELHTLVGAGATSGAMDASNL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPALARGEIRCIGATTLDEYRQHIEKDPALARRFQPVFISQPTETDTISILRGLKERYE
HHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHH
VHHGIRITDGAIIAAATLSNRYITDRFLPDKAIDLIDEAASRVRIEMDSKPEVVDELERK
HHCCEEEECCCEEEEEHHCCCCHHHHCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHH
IIQLKIESEALKKESDENSKQRLKKINEEIESLNSKFADLSSKWQMEKNKIAKIQETAEK
HHHHEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDNARKELELAQRNGNLGRAGELMYGVIPQLENELKNQEKVTDSFLKKEVTESDIANIVS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KWTGIPVDNMMHSEKEKLLNMENEIGKRVIGQRDAIEAISNAVRRSRSGVQDTNRPFGSF
HHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LFLGPTGVGKTELAKSLAEFLFDDQSALLRFDMSEYMEKHSVSKLIGAPPGYVGYEQGGR
EEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LTEAVRRRPYQVILFDEIEKANPDIFNILLQILDEGRLTDSHGKLIDFRNTILILTSNLG
HHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCC
AEMMLRGATESVKDEVMKIVKSAFRPEFLNRLDEIIIFHSLTKDDIDKIIDVQFSYLQKM
HHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAKRKLSISLSQEAKGLIAQTGYDPEYGARPLKRVIQECIQNNLAKLVLSGEIVEGDELI
HHHHHHCEEECHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCEEE
VYTSGNEILVKKI
EEECCCEEEEEEC
>Mature Secondary Structure
MDLNKFTEKAKSLIQRAQMKALGAGHQIFMPEHLLKVMLEDESGLVQDLIGTCGGNVQNI
CCHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCHHHHHHHHCCCCHHHH
SDAVSSTIKKFPVVGGPGSVGLQLSREVAKVFDDSIGIARKNKDTFVTVERLLQGLAAQK
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DEIVGKILAEGGVTPQKLNSVIAEMRKGGSADSPNSEEKLNAAKRYTKDITELAMQGKLD
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
PVIGRDEEIRRTMQVSLRRTKNNPVLIGEPGVGKTAIVEGLANRIVANDVPLGLRDAKVL
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHH
ALDLGALIAGTKFRGEFEERLKAVINEILKAEGKVILFIDELHTLVGAGATSGAMDASNL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPALARGEIRCIGATTLDEYRQHIEKDPALARRFQPVFISQPTETDTISILRGLKERYE
HHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHH
VHHGIRITDGAIIAAATLSNRYITDRFLPDKAIDLIDEAASRVRIEMDSKPEVVDELERK
HHCCEEEECCCEEEEEHHCCCCHHHHCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHH
IIQLKIESEALKKESDENSKQRLKKINEEIESLNSKFADLSSKWQMEKNKIAKIQETAEK
HHHHEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDNARKELELAQRNGNLGRAGELMYGVIPQLENELKNQEKVTDSFLKKEVTESDIANIVS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KWTGIPVDNMMHSEKEKLLNMENEIGKRVIGQRDAIEAISNAVRRSRSGVQDTNRPFGSF
HHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LFLGPTGVGKTELAKSLAEFLFDDQSALLRFDMSEYMEKHSVSKLIGAPPGYVGYEQGGR
EEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LTEAVRRRPYQVILFDEIEKANPDIFNILLQILDEGRLTDSHGKLIDFRNTILILTSNLG
HHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCC
AEMMLRGATESVKDEVMKIVKSAFRPEFLNRLDEIIIFHSLTKDDIDKIIDVQFSYLQKM
HHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAKRKLSISLSQEAKGLIAQTGYDPEYGARPLKRVIQECIQNNLAKLVLSGEIVEGDELI
HHHHHHCEEECHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCEEE
VYTSGNEILVKKI
EEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA