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The map label for this gene is rnhA [H]

Identifier: 190570726

GI number: 190570726

Start: 283821

End: 284354

Strand: Reverse

Name: rnhA [H]

Synonym: WPa_0279

Alternate gene names: 190570726

Gene position: 284354-283821 (Counterclockwise)

Preceding gene: 190570727

Following gene: 190570725

Centisome position: 19.18

GC content: 36.52

Gene sequence:

>534_bases
ATGGACAAAAAGAAGGTTATAATATATACAGATGGAGCTTGTTCTGGAAACCCTGGTCCCGGTGGATGGGCAGCCGTGGT
AATGTATGAAAATAAAAGTGTCTTTATCAAAAAACGTATCTCTGGAGGTGAAGAAAACACAACAAATAACAAGATGGAGC
TAAAGGCTGTAATTAATGGACTAAAGATGTTAAAAATTTCCTGCAAAGTTATTGTACACACTGATAGTCAGTATATTAAA
CAAGGTATAACAGAGTGGATCAACAAGTGGAAAACAAATGGTTGGAAGACAGCCGATAAAAAGCCAGTGAAGAACAGAGA
ATTATGGCAGGAACTAGATGAAGTTGCTTTGCAGCATGATATTAATTGGAAGTGGGTTAGAGCTCACAACGGTAATATGT
ACAATGAGGAGGCAGATAGACTTGCTAGAAAGGAATCTAAAAATCTAAAATATAGAGATTGTGAGGTCAAGAAATCACCA
AAAAATAGAGGGAACTCTAAGTTTCATAGATTGGGTGGAGTATTATGGCAATAA

Upstream 100 bases:

>100_bases
ACAGGTTCATTAATCTCCCACACCTTTACAGGATAAAGATCTTGAAACTTAGTGTCAACATTTCAGTAGCAAATTTATGT
TAAATCTATATCCTGTCAAT

Downstream 100 bases:

>100_bases
TTCTTTTAGATACAAAAACAATAAATCGTATAGCAGCAGGTGAAGTAATCGAAAGGCCAGCAAGTGTAGTAAAGGAATTA
GTAGAAAATGCAATAGATGC

Product: ribonuclease h

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MDKKKVIIYTDGACSGNPGPGGWAAVVMYENKSVFIKKRISGGEENTTNNKMELKAVINGLKMLKISCKVIVHTDSQYIK
QGITEWINKWKTNGWKTADKKPVKNRELWQELDEVALQHDINWKWVRAHNGNMYNEEADRLARKESKNLKYRDCEVKKSP
KNRGNSKFHRLGGVLWQ

Sequences:

>Translated_177_residues
MDKKKVIIYTDGACSGNPGPGGWAAVVMYENKSVFIKKRISGGEENTTNNKMELKAVINGLKMLKISCKVIVHTDSQYIK
QGITEWINKWKTNGWKTADKKPVKNRELWQELDEVALQHDINWKWVRAHNGNMYNEEADRLARKESKNLKYRDCEVKKSP
KNRGNSKFHRLGGVLWQ
>Mature_177_residues
MDKKKVIIYTDGACSGNPGPGGWAAVVMYENKSVFIKKRISGGEENTTNNKMELKAVINGLKMLKISCKVIVHTDSQYIK
QGITEWINKWKTNGWKTADKKPVKNRELWQELDEVALQHDINWKWVRAHNGNMYNEEADRLARKESKNLKYRDCEVKKSP
KNRGNSKFHRLGGVLWQ

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=36.0544217687075, Blast_Score=90, Evalue=9e-19,
Organism=Escherichia coli, GI1786408, Length=150, Percent_Identity=53.3333333333333, Blast_Score=166, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17505482, Length=136, Percent_Identity=33.0882352941176, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115532542, Length=151, Percent_Identity=33.7748344370861, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45552509, Length=148, Percent_Identity=38.5135135135135, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17137476, Length=148, Percent_Identity=38.5135135135135, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 20448; Mature: 20448

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKVIIYTDGACSGNPGPGGWAAVVMYENKSVFIKKRISGGEENTTNNKMELKAVING
CCCCEEEEEECCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
LKMLKISCKVIVHTDSQYIKQGITEWINKWKTNGWKTADKKPVKNRELWQELDEVALQHD
HHEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
INWKWVRAHNGNMYNEEADRLARKESKNLKYRDCEVKKSPKNRGNSKFHRLGGVLWQ
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MDKKKVIIYTDGACSGNPGPGGWAAVVMYENKSVFIKKRISGGEENTTNNKMELKAVING
CCCCEEEEEECCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
LKMLKISCKVIVHTDSQYIKQGITEWINKWKTNGWKTADKKPVKNRELWQELDEVALQHD
HHEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
INWKWVRAHNGNMYNEEADRLARKESKNLKYRDCEVKKSPKNRGNSKFHRLGGVLWQ
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA