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The map label for this gene is tolB [H]

Identifier: 190570675

GI number: 190570675

Start: 235266

End: 236522

Strand: Direct

Name: tolB [H]

Synonym: WPa_0225

Alternate gene names: 190570675

Gene position: 235266-236522 (Clockwise)

Preceding gene: 190570674

Following gene: 190570678

Centisome position: 15.87

GC content: 32.06

Gene sequence:

>1257_bases
ATGAAGCTATTCGTTCAACTGGTGTTATTTATTTCGTTATTTATTCCTTATTCCACAAAAGCTGTTTTATATGTTGATAT
AAAAAAAAGCAATATTGGTAATATTGATCTTGTTGTATCTAAATGTGCATGCAAGACAGAAGTGGAAAATGAGCTAAGTG
AAAGCATCACAAAAGTAATTGAAACAAATCTATCTAATTGTGGCTTATTTAATGTGAAACGTGACGCGAAAGTTGAGTCT
TGGAAAAGCGATACTGTAGTCACAATAAGTTTAAGTGAAGTATCAAACAGAAATTTAGAGCTATCTTTTCGTTTATCTGA
CAGCTTTACAAACAGAGAATTGCTTACTCAGTCGGTTGTTTTTCTAGCAAAAGACTGGAGGAAAATTAGTCATCTTGTTT
CAGATCTGATACATGATAGATTGATTGGTGAGAAAGGGCATTTCAATACAAAAATTACATATATCGCTGAAGAAAAAGAT
AGCAATTACAAATCCGTCCGTAAAATTGCTGTGATGAATCAAGATGGAAGTAATATAAAGTACTTAACAAATGGTGAGAA
ATTTGTGTCAACACCAAGATTTTCACCAAATGGAAAGGGTATTGTTTATATATCATACACAAATGGTAAAAGTTATATAA
TATTAAAAAATTTAAAAGATAACACTGAATCAATAATCAGCGCATTTGAAGGAGTTATTTCTGCACCAAGATTTTCTCCT
GATGGTAAATCTCTTTTTATTTCCCACTCATTGAGTGGTGAAACAAATATATTATCTCTAGACTTAAGCAGTAAACGAAC
AAAAAAAATTACTAAAGGTTCAGCTATAAGTACTTCTCCCTCTTTGTCTCCAGATCAAAAGTACATGGTTTTTAGTTCTG
ATATAAGTGGAAGTCAGCAACTATATATCATAGATTTCACTAAAAAAAGCAAAAAACCCAAGAGAATTAGTTTTGGAAAT
GGAAGATATGCTACACCTGTTTGGTCGCCAAAAGGAGATCTGATAGCTTTTACAAAAATTCAGTCAGGAAAATTTTACAT
AGGAGTTATGAAGCCAGATGGCAAAGAAGAGCGCTTGCTTTCAGAGGGACATAAAATTGAATCTCCGGCATGGCTACCAA
ATGGCAGAGAAATTATCTTTACTAACGCAGAATCACCAAGCAACTCTAAATTATATTTAGTAGATTTAGTAAAGAAAAAT
CAAAAAATGGTTTTTACTCCAACGAATGCTTCTTTACCAGATTGGTCTTATTTTTGA

Upstream 100 bases:

>100_bases
GTGTCAAGTACTGCAGAAATTGATTTACTCTACTGTCATGCAGTTTAACTTCAAAAGTAAGCTATTTGCTGAGATGAAAG
GAGGTAATGCAAGAAATCTA

Downstream 100 bases:

>100_bases
AAGTAGATCTATAAAATCGGTATAAATCTTGCTTTATCTTAATCGTTTATAACCTTCGGTACTACGAAATACCCATGCTC
TGATTTTGTATTGGATAATA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKLFVQLVLFISLFIPYSTKAVLYVDIKKSNIGNIDLVVSKCACKTEVENELSESITKVIETNLSNCGLFNVKRDAKVES
WKSDTVVTISLSEVSNRNLELSFRLSDSFTNRELLTQSVVFLAKDWRKISHLVSDLIHDRLIGEKGHFNTKITYIAEEKD
SNYKSVRKIAVMNQDGSNIKYLTNGEKFVSTPRFSPNGKGIVYISYTNGKSYIILKNLKDNTESIISAFEGVISAPRFSP
DGKSLFISHSLSGETNILSLDLSSKRTKKITKGSAISTSPSLSPDQKYMVFSSDISGSQQLYIIDFTKKSKKPKRISFGN
GRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLLSEGHKIESPAWLPNGREIIFTNAESPSNSKLYLVDLVKKN
QKMVFTPTNASLPDWSYF

Sequences:

>Translated_418_residues
MKLFVQLVLFISLFIPYSTKAVLYVDIKKSNIGNIDLVVSKCACKTEVENELSESITKVIETNLSNCGLFNVKRDAKVES
WKSDTVVTISLSEVSNRNLELSFRLSDSFTNRELLTQSVVFLAKDWRKISHLVSDLIHDRLIGEKGHFNTKITYIAEEKD
SNYKSVRKIAVMNQDGSNIKYLTNGEKFVSTPRFSPNGKGIVYISYTNGKSYIILKNLKDNTESIISAFEGVISAPRFSP
DGKSLFISHSLSGETNILSLDLSSKRTKKITKGSAISTSPSLSPDQKYMVFSSDISGSQQLYIIDFTKKSKKPKRISFGN
GRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLLSEGHKIESPAWLPNGREIIFTNAESPSNSKLYLVDLVKKN
QKMVFTPTNASLPDWSYF
>Mature_418_residues
MKLFVQLVLFISLFIPYSTKAVLYVDIKKSNIGNIDLVVSKCACKTEVENELSESITKVIETNLSNCGLFNVKRDAKVES
WKSDTVVTISLSEVSNRNLELSFRLSDSFTNRELLTQSVVFLAKDWRKISHLVSDLIHDRLIGEKGHFNTKITYIAEEKD
SNYKSVRKIAVMNQDGSNIKYLTNGEKFVSTPRFSPNGKGIVYISYTNGKSYIILKNLKDNTESIISAFEGVISAPRFSP
DGKSLFISHSLSGETNILSLDLSSKRTKKITKGSAISTSPSLSPDQKYMVFSSDISGSQQLYIIDFTKKSKKPKRISFGN
GRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLLSEGHKIESPAWLPNGREIIFTNAESPSNSKLYLVDLVKKN
QKMVFTPTNASLPDWSYF

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=383, Percent_Identity=24.0208877284595, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 46866; Mature: 46866

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFVQLVLFISLFIPYSTKAVLYVDIKKSNIGNIDLVVSKCACKTEVENELSESITKVI
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHH
ETNLSNCGLFNVKRDAKVESWKSDTVVTISLSEVSNRNLELSFRLSDSFTNRELLTQSVV
HCCCCCCCEEEECCCCCCCCCCCCEEEEEEEHHCCCCCEEEEEEECCCCCCHHHHHHHHH
FLAKDWRKISHLVSDLIHDRLIGEKGHFNTKITYIAEEKDSNYKSVRKIAVMNQDGSNIK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHEEEEEEECCCCCCEE
YLTNGEKFVSTPRFSPNGKGIVYISYTNGKSYIILKNLKDNTESIISAFEGVISAPRFSP
EEECCCCEEECCCCCCCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
DGKSLFISHSLSGETNILSLDLSSKRTKKITKGSAISTSPSLSPDQKYMVFSSDISGSQQ
CCCEEEEEECCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEE
LYIIDFTKKSKKPKRISFGNGRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLL
EEEEEECCCCCCCCEEECCCCEEECCEECCCCCEEEEEEEECCEEEEEEECCCCHHHHHH
SEGHKIESPAWLPNGREIIFTNAESPSNSKLYLVDLVKKNQKMVFTPTNASLPDWSYF
HCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKLFVQLVLFISLFIPYSTKAVLYVDIKKSNIGNIDLVVSKCACKTEVENELSESITKVI
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHH
ETNLSNCGLFNVKRDAKVESWKSDTVVTISLSEVSNRNLELSFRLSDSFTNRELLTQSVV
HCCCCCCCEEEECCCCCCCCCCCCEEEEEEEHHCCCCCEEEEEEECCCCCCHHHHHHHHH
FLAKDWRKISHLVSDLIHDRLIGEKGHFNTKITYIAEEKDSNYKSVRKIAVMNQDGSNIK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHEEEEEEECCCCCCEE
YLTNGEKFVSTPRFSPNGKGIVYISYTNGKSYIILKNLKDNTESIISAFEGVISAPRFSP
EEECCCCEEECCCCCCCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
DGKSLFISHSLSGETNILSLDLSSKRTKKITKGSAISTSPSLSPDQKYMVFSSDISGSQQ
CCCEEEEEECCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEE
LYIIDFTKKSKKPKRISFGNGRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLL
EEEEEECCCCCCCCEEECCCCEEECCEECCCCCEEEEEEEECCEEEEEEECCCCHHHHHH
SEGHKIESPAWLPNGREIIFTNAESPSNSKLYLVDLVKKNQKMVFTPTNASLPDWSYF
HCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA